Due to the global public health crisis caused by the coronavirus disease 2019 (COVID-19) pandemic, the importance of vaccine development has increased. In particular, a rapid supply of vaccines and prompt deployment of vaccination programs are essential to prevent and overcome the spread of COVID-19. As a part of the vaccine regulations, national lot release is regulated by the responsible authorities, and this process involves the assessment of the lot before a vaccine is marketed. A lot can be released for use when both summary protocol (SP) review and quality control testing are complete. Accelerated lot release is required to distribute COVID-19 vaccines in a timely manner. In order to expedite the process by simultaneously undertaking the verification of quality assessment and application for approval, it is necessary to prepare the test methods before marketing authorization. With the prolonged pandemic and controversies regarding the effectiveness of the COVID-19 vaccine against new variants, public interest for the development of a new vaccine are increasing. Domestic developers have raised the need to establish standard guidance on the requirements for developing COVID-19 vaccine. This paper presents considerations for quality control in the manufacturing process, test items, and SP content of viral vector vaccines.
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Objectives
In this paper we present an age-structured epidemiological model for Chagas disease. This model includes the interactions between human and vector populations that transmit Chagas disease. Methods
The human population is divided into age groups since the proportion of infected individuals in this population changes with age as shown by real prevalence data. Moreover, the age-structured model allows more accurate information regarding the prevalence, which can help to design more specific control programs. We apply this proposed model to data from the country of Venezuela for two periods, 1961–1971, and 1961–1991 taking into account real demographic data for these periods. Results
Numerical computer simulations are presented to show the suitability of the age-structured model to explain the real data regarding prevalence of Chagas disease in each of the age groups. In addition, a numerical simulation varying the death rate of the vector is done to illustrate prevention and control strategies against Chagas disease. Conclusion
The proposed model can be used to determine the effect of control strategies in different age groups.
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Objectives
Bacteriophage-encoded endolysins are a group of enzymes that act by digesting the peptidoglycan of bacterial cell walls. LysK has been reported to lyse live staphylococcal cultures. LysK proteins containing only the cysteine/histidine-dependent amidohydrolase/peptidase (CHAP) domain has the capability to show lytic activity against live clinical staphylococcal isolates, including methicillin-resistant <i>Staphylococcus aureus</i> (MRSA). The aim of this study was to clone and express LysK-CHAP domain in <i>Escherichia coli BL21 (DE3)</i> using pET22b as a secretion vector. The pET22b plasmid was used, which encoded a pelB secretion signal under the control of the strong bacteriophage T7 promoter. Methods
The <i>E. coli</i> cloning strains DH5α and BL21 (DE3) were grown at 37°C with aeration in the Luria-Bertani medium. A plasmid encoding LysK-CHAP in a pET22b backbone was constructed. The pET22b vector containing LysK-CHAP sequences were digested with NcoI and HindIII restriction enzymes. Cloning accuracy was confirmed by electrophoresis. The pET22b-LysK plasmid was used to transform the <i>E. coli</i> strain BL21. Isopropyl β-<sc>d</sc>-1-thiogalactopyranoside (IPTG) was added to a final concentration of 1mM to induce T7 RNA polymerase-based expression. Finally, western blot confirmed the expression of target protein. Results
In this study, after double digestion of pEX and pET22b vectors with HindIII and NcoI, LysK gene was cloned into two HindIII and NcoI sites in pET22b vector, and then transformed to <i>E. coli DH5α</i>. Cloning was confirmed with double digestion and analyzed with agarose gel. The recombinant pET22b-LysK plasmid was transformed to <i>E. coli BL21</i> and the expression was induced by IPTG. The expression was confirmed by Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and western blotting method. Observation of a 28.5 kDa band confirmed LysK protein expression. Conclusion
In the present study, LysK-CHAP domain was successfully cloned and expressed at the pET22b vector and <i>E. coli BL21 (DE3)</i>.
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Objectives
Tuberculosis (TB) is the leading infectious disease in the developing world. Delayed-type hypersensitivity skin test diagnoses TB using tuberculin purified protein derivative (PPD), but this test is incapable of distinguishing <i>Mycobacterium tuberculosis</i> (MTB) infection from bacillus Calmette–Guérin (BCG) vaccination or an infection caused by nontuberculous mycobacteria (NTM). This study was performed to evaluate the use of recombinant early secretory antigenic target 6 (rESAT-6), a secretory protein found only in MTB, <i>Mycobacterium bovis</i>, and few other mycobacterial species, as a skin marker for MTB in guinea pigs. Methods
We prepared recombinant MTB ESAT-6 and evaluated its use as a specific antigen for MTB in guinea pigs. Results
Our results show that the purified MTB rESAT-6 antigen is capable of inducing a positive reaction only in guinea pigs sensitized to MTB. No such reaction was observed in the animals sensitized to <i>M. bovis</i>, BCG vaccination, or NTM (<i>Mycobacterium avium</i>). Conclusion
Our study results confirm that the ESAT-6 antigen is more specific to MTB infection than PPD and could be used in more specific skin tests for detection of MTB in large animals and in humans.
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Objectives
To evaluate the ovicidal and oviposition deterrent activities of five medicinal plant extracts namely <i>Aegle marmelos</i> (Linn.), <i>Limonia acidissima</i> (Linn.), <i>Sphaeranthus indicus</i> (Linn.), <i>Sphaeranthus amaranthoides</i> (burm.f), and <i>Chromolaena odorata</i> (Linn.) against <i>Culex quinquefasciatus</i> and <i>Aedes aegypti</i> mosquitoes. Three solvents, namely hexane, ethyl acetate, and methanol, were used for the preparation of extracts from each plant. Methods
Four different concentrations—62.5 parts per million (ppm), 125 ppm, 250 ppm, and 500 ppm—were prepared using acetone and tested for ovicidal and oviposition deterrent activities. One-way analysis of variance (ANOVA) was used to determine the significance of the treatments and means were separated by Tukey's test of comparison. Results
Among the different extracts of the five plants screened, the hexane extract of <i>L. acidissima</i> recorded the highest ovicidal activity of 79.2% and 60% at 500 ppm concentration against the eggs of <i>Cx. quinquefasciatus</i> and <i>Ae. aegypti</i>, respectively. Similarly, the same hexane extract of <i>L. acidissima</i> showed 100% oviposition deterrent activity at all the tested concentrations against <i>Cx. quinquefasciatus</i> and <i>Ae. aegypti</i> adult females. Conclusion
It is concluded that the hexane extract of <i>L. acidissima</i> could be used in an integrated mosquito management program.
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Objectives
Classification of breast cancer patients into different risk classes is very important in clinical applications. It is estimated that the advent of high-dimensional gene expression data could improve patient classification. In this study, a new method for transforming the high-dimensional gene expression data in a low-dimensional space based on wavelet transform (WT) is presented. Methods
The proposed method was applied to three publicly available microarray data sets. After dimensionality reduction using supervised wavelet, a predictive support vector machine (SVM) model was built upon the reduced dimensional space. In addition, the proposed method was compared with the supervised principal component analysis (PCA). Results
The performance of supervised wavelet and supervised PCA based on selected genes were better than the signature genes identified in the other studies. Furthermore, the supervised wavelet method generally performed better than the supervised PCA for predicting the 5-year survival status of patients with breast cancer based on microarray data. In addition, the proposed method had a relatively acceptable performance compared with the other studies. Conclusion
The results suggest the possibility of developing a new tool using wavelets for the dimension reduction of microarray data sets in the classification framework.
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Objectives
Until the early 2000s, lymphatic filariasis would commonly break out in the coastal areas in Korea. Through steady efforts combining investigation and treatment, filariasis was officially declared eradicated in 2008. This study surveyed the density of vector species of filariasis in past endemic areas, and inspected filariasis DNA from collected mosquitoes for protection against the reemergence of filariasis. Methods
Between May and October 2009, mosquitoes were caught using the black night trap in past endemic coastal areas: Gyeongsangnam-do, Jeollanamdo, and Jeju-do. The collected mosquitoes were identified, and the extracted DNA from the collected vector mosquitoes was tested by polymerase chain reaction for <i>Brugia malayi</i> filariasis. Results
<i>Ochletotatus togoi, Anophel es (Hyrcanus)</i> group and <i>Culex pipiens</i> were most frequently caught in Jeollanam-do (Geomun Island, Bogil Island, Heuksan Island), Jeju-do (Namone-ri, Wimi-ri). and Gyeongsangnam-do (Maemul Island). DNA of <i>B malayi</i> was not found in <i>Och Togoi</i> and <i>An (Hyrcanus)</i> group as main vectors of filariasis. Conclusion
Lymphatic filariasis was not found in the vector mosquitoes collected in past endemic areas. However, considering that the proportion of vector species is quite high, there is a potential risk that filariasis could be reemerging through overseas travel or trade. Thus, there is a need to continuously monitor vector mosquitoes of lymphatic filariasis.
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Global warming has various effects on human health. The main indirect effects are on infectious diseases. Although the effects on infectious diseases will be detected worldwide, the degree and types of the effect are different, depending on the location of the respective countries and socioeconomical situations.Among infectious diseases, water- and foodborne infectious diseases and vector-borne infectious diseases are two main categories that are forecasted to be most affected. The effect on vector-borne infectious diseases such as malaria and dengue fever is mainly because of the expansion of the infested areas of vector mosquitoes and increase in the number and feeding activity of infected mosquitoes. There will be increase in the number of cases with water- and foodborne diarrhoeal diseases.Even with the strongest mitigation procedures, global warming cannot be avoided for decades. Therefore, implementation of adaptation measures to the effect of global warming is the most practical action we can take. It is generally accepted that the impacts of global warming on infectious diseases have not been apparent at this point yet in East Asia. However, these impacts will appear in one form or another if global warming continues to progress in future. Further research on the impacts of global warming on infectious diseases and on future prospects should be conducted.
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