aNational Biobank of Korea, Center for Genome Sciences, Korea National Institute of Health, Korea Centers for Disease Control and Prevention, Cheongju, Korea
bDepartment of Laboratory Medicine, Pusan National University School of Medicine, Busan, Korea
Copyright © 2016 Korea Centers for Disease Control and Prevention. Published by Elsevier Korea LLC.
This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
Sample type | No. of freeze–thaw cycles |
proGRP concentrations (pg/mL) |
|||
---|---|---|---|---|---|
Range | Mean (±SD) | Change (%)∗ | p† | ||
Plasma | 1 | 27.6–91.6 | 41.3 (±14.9) | — | |
2 | 28.4–84.8 | 40.1 (±13.6) | −2.9 | 0.006 | |
3 | 28.6–85.6 | 40.5 (±13.9) | −1.9 | 0.014 | |
4 | 26.2–87.8 | 40.0 (±14.1) | −3.1 | 0.003 | |
Serum | 1 | 24.3–83.4 | 36.5 (±13.6) | — | |
2 | 21.9–79.9 | 34.9 (±13.6) | −4.4 | <0.001 | |
3 | 19.5–77.2 | 34.0 (±13.1) | −6.8 | <0.001 | |
4 | 18.2–77.9 | 32.8 (±13.5) | −10.1 | <0.001 |
Sample type | No. of freeze–thaw cycles | proGRP concentrations (pg/mL) | |||
---|---|---|---|---|---|
Range | Mean (±SD) | Change (%) | p | ||
Plasma | 1 | 27.6–91.6 | 41.3 (±14.9) | — | |
2 | 28.4–84.8 | 40.1 (±13.6) | −2.9 | 0.006 | |
3 | 28.6–85.6 | 40.5 (±13.9) | −1.9 | 0.014 | |
4 | 26.2–87.8 | 40.0 (±14.1) | −3.1 | 0.003 | |
Serum | 1 | 24.3–83.4 | 36.5 (±13.6) | — | |
2 | 21.9–79.9 | 34.9 (±13.6) | −4.4 | <0.001 | |
3 | 19.5–77.2 | 34.0 (±13.1) | −6.8 | <0.001 | |
4 | 18.2–77.9 | 32.8 (±13.5) | −10.1 | <0.001 |
Sample processing time (min) | Sample type | No. of freeze–thaw cycles | |||
---|---|---|---|---|---|
1 | 2 | 3 | 4 | ||
Thawing time at 37°C | Plasma | 2.2 | 4.2 | 6.5 | 8.5 |
(0.4) | (0.4) | (0.8) | (0.8) | ||
Serum | 2.3 | 4.3 | 6.7 | 8.7 | |
(0.8) | (0.8) | (1.0) | (1.0) | ||
Exposure time at room temperature | Plasma | 1.3 | 1.9 | 2.4 | 3.2 |
(0.2) | (0.6) | (0.8) | (0.9) | ||
Serum | 1.1 | 1.9 | 2.2 | 2.9 | |
(0.1) | (0.4) | (0.7) | (1.0) |
proGRP = progastrin-releasing peptide; SD = standard deviation. Percentage change, with a “+” for an increase and a “−” for a decrease relative to baseline (1 cycle). The
Data are presented as mean ±SD.