Division of Enteric Bacterial Infections, Korea National Institute of Health, Osong, Korea
© 2013 Published by Elsevier B.V. on behalf of Korea Centers for Disease Control and Prevention.
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Species (No. isolates) | Gene target | ||
---|---|---|---|
hlyA | tlh | vvhA | |
Vibrio cholerae (10) | + | – | – |
Vibrio parahaemolyticus (10) | – | + | – |
Vibrio vulnificus (10) | – | – | + |
Vibrio mimicus (2) | – | – | – |
Vibrio alginolyticus (1) | – | – | – |
ETEC ATCC 43896 | – | – | – |
ETEC (5) | – | – | – |
EHEC ATCC 43895 | – | – | – |
EAEC (3) | – | – | – |
Shigella spp. (2) | – | – | – |
Shigella flexneri (3) | – | – | – |
Shigella sonnei (2) | – | – | – |
Salmonella spp. (2) | – | – | – |
Salmonella enteritidis (2) | – | – | – |
Salmonella typhimurium (2) | – | – | – |
Vibrio spp. | Primer or probe sequence (5′ to 3′) | Target gene | Amplicon size (bp) | |
---|---|---|---|---|
V. cholerae | VCF | GCGTTGGGAGTGGCGTAA | hlyA | 57 |
VCR | GGACTCGCCGCTGTAGACA | |||
VCP | FAM-AGCACAGATGAATTGACCAMG-BNHQ | |||
V. parahaemolyticus | VPF | AACCGTGGCGTTCCAGAA | tlh | 58p |
VPR | CCGTCAAACGAATCAGTGCTT | |||
VPP | VIC-TGAAAGCGGATTATGC-MGB | |||
V. vulnificus | VVF | GATCGTTGTTTGACCGTAAACG | vvhA | 79p |
VVR | TGCTAAGTTCGCACCACACTGT | |||
VVP | NED-CAAAACGCTCACAGTCG-MGB |
Vibrio spp. | No. positive (%) | ||
---|---|---|---|
2007 | 2006 | ||
RT-PCR (n = 2,729) | Isolates (n = 2,769) | Isolates (n = 5,445) | |
V. cholerae | 330 (10.3) | 228 (5.6) | 213 (3.3) |
V. parahaemolyticus | 2085 (64.7) | 1501 (37) | 1893 (29.3) |
V. vulnificus | 621 (19.3) | 180 (4.4) | 106 (1.6) |
EAEC = enteroaggregative
F = forward primer; P = probe; R = reverse primer.
Data are presented as