Objectives
Production of carbapenemase, especially <i>Klebsiella pneumoniae</i> carbapenemases (KPC), is one of the antibiotic resistance mechanisms of Enterobacteriaceae such as <i>Klebsiella oxytoca</i>. This study aimed to investigate and identify KPC-producing <i>K. oxytoca</i> isolates using molecular and phenotypic methods. Methods
A total of 75 isolates of <i>K. oxytoca</i> were isolated from various clinical samples, and were verified as <i>K. oxytoca</i> after performing standard microbiological tests and using a polymerase chain reaction (PCR) method. An antibiotic susceptibility test was performed using a disc diffusion method according to the Clinical and Laboratory Standards Institute guidelines. CHROMagar KPC chromogenic culture media was used to examine and confirm the production of the carbapenemase enzyme in <i>K. oxytoca</i> isolates; in addition, PCR was used to evaluate the presence of <i>bla</i><sub>KPC</sub> gene in <i>K. oxytoca</i> strains. Results
Of a total of 75 <i>K. oxytoca</i> isolates, one multidrug resistant strain was isolated from the urine of a hospitalized woman. This strain was examined to assess its ability to produce carbapenemase enzyme; it produced a colony with a blue metallic color on the CHROMagar KPC chromogenic culture media. In addition, the <i>bla</i><sub>KPC</sub> gene was confirmed by PCR. After sequencing, it was confirmed and deposited in GenBank. Conclusion
To date, many cases of KPC-producing Enterobacteriaceae, in particular <i>K. pneumoniae</i>, have been reported in different countries; there are also some reports on the identification of KPC-producing <i>K. oxytoca</i>. Therefore, to prevent the outbreak of nosocomial infections, the early detection, control, and prevention of the spread of these strains are of great importance.
Citations
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