| Mohammad Reza Arabestani | 2 Articles |
<sec>
<title>Objectives</title>
<p>The purpose of this study was to determine the presence of <italic>IMP</italic> and <italic>OXA</italic> genes in clinical strains of <italic>Pseudomonas aeruginosa</italic> (<italic>P. aeruginosa</italic>) that are carriers of the <italic>ampC</italic> gene.</p></sec>
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<title>Methods</title>
<p>In this study, 105 clinical isolates of <italic>P. aeruginosa</italic> were collected. Antibiotic resistance patterns were determined using the disk diffusion method. The strains carrying AmpC enzymes were characterized by a combination disk method. Multiplex-PCR was used to identify resistance and virulence genes, chi-square test was used to determine the relationship between variables.</p></sec>
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<title>Results</title>
<p>Among 105 isolates of <italic>P. aeruginosa</italic>, the highest antibiotic resistance was to cefotaxime and aztreonam, and the least resistance was to colictin and ceftazidime. There were 49 isolates (46.66%) that showed an AmpC phenotype. In addition, the frequencies of the resistance genes were; <italic>OXA48</italic> gene 85.2%, <italic>OXA199, 139</italic> 3.8%, <italic>OXA23</italic> 3.8%, <italic>OXA2</italic> 66.6%, <italic>OXA10</italic> 3.8%, <italic>OXA51</italic> 85.2% and <italic>OXA58</italic> 3.8%. The <italic>IMP27</italic> gene was detected in 9 isolates (8.57%) and the <italic>IMP3.34</italic> was detected in 11 isolates (10.47%). Other genes detected included; <italic>lasR</italic> (17.1%), <italic>lasB</italic> (18%) and <italic>lasA</italic> (26.6%). There was a significant relationship between virulence factors and the <italic>OX</italic> and <italic>IMP</italic> genes (<italic>p</italic> ≤ 0.05).</p></sec>
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<title>Conclusion</title>
<p>The relationship between antibiotic resistance and virulence factors observed in this study could play an important role in outbreaks associated with <italic>P. aeruginosa</italic> infections.</p></sec>
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<sec><title>Objectives</title><p>Integrons are thought to play an important role in the spread of antibiotic resistance. This study investigates class 1 and 2 integron-positive methicillin-resistant coagulase-negative staphylococci strains isolated in Iran and characterizes their patterns of antimicrobial resistance.</p></sec><sec><title>Methods</title><p>Hundred clinical isolates of coagulase-negative staphylococci were characterized for integron content and staphylococcal cassette chromosome <italic>mec</italic> (SCCmec) type.</p></sec><sec><title>Results</title><p>Sixteen isolates carried class 1 (<italic>intI1</italic>) integrons and four isolates carried class 2 (<italic>intI2</italic>) integrons. One resistance gene array was identified among the class 1 integrons (<italic>aadA1</italic> cassette). The distribution of SCCmec types in 50 methicillin-resistant coagulase-negative staphylococci strains showed that SCCmec types III and V dominated among the tested strains.</p></sec><sec><title>Conclusion</title><p>This is the first report of methicillin-resistant coagulase-negative staphylococci strains that carry two mobile genetic elements, including class 1 and 2 integrons and SCCmec, in Iran.</p></sec>
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