| Farhad Nikkhahi | 1 Article |
Objectives
Gastrointestinal tract infection is still one of the serious public health problems in many geographic areas and is endemic in most countries including Iran. Early detection of the gastrointestinal tract pathogens can be extremely important. The aim of the current study was to apply a shortened time-multiplex polymerase chain reaction (PCR) for rapid and simultaneous detection of <i>Salmonella</i> spp., <i>Shigella</i> spp., and <i>Vibrio cholera</i>. Methods The standard and clinical strains of <i>Salmonella</i> spp., <i>Shigella</i> spp., and <i>V. cholerae</i> were used in the assay. Multiplex PCR was performed and optimized based on amplification of <i>invA</i>, putative integrase, and <i>ompW</i> genes for detecting <i>Salmonella</i> spp., <i>Shigella</i> spp., and <i>V. cholerae</i>, respectively. The specificity of the assay was evaluated by testing 12 different bacterial species. Results Only <i>Salmonella</i> spp., <i>Shigella</i> spp., and <i>V. cholerae</i> strains had positive results when subjected to the assay using multiplex PCR. The assay showed a high sensitivity, and no amplification products were observed in multiplex PCR with any of the other microorganisms. Conclusion Our study indicated that the <i>invA</i>, putative integrase, and <i>ompW</i>-based multiplex PCR assay appears to be an efficient method for rapid and simultaneous detection of <i>Salmonella</i> spp., <i>Shigella</i> spp., and <i>V. cholerae.</i> Citations
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