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Brief Report
Replication kinetics and infectivity of SARS-CoV-2 Omicron variant sublineages recovered in the Republic of Korea
Jeong-Min Kim, Dongju Kim, Jee Eun Rhee, Cheon Kwon Yoo, Eun-Jin Kim
Osong Public Health Res Perspect. 2024;15(3):260-264.   Published online June 27, 2024
DOI: https://doi.org/10.24171/j.phrp.2023.0216
  • 1,191 View
  • 29 Download
  • 1 Crossref
Graphical AbstractGraphical Abstract AbstractAbstract PDFSupplementary Material
Objectives
We analyzed the correlation between the infectivity and transmissibility of the severe acute respiratory syndrome coronavirus 2 Omicron sublineages BA.1, BA. 2, BA.4, and BA.5. Methods: We assessed viral replication kinetics and infectivity at the cellular level. Nasopharyngeal and oropharyngeal specimens were obtained from patients with coronavirus disease 2019, confirmed using whole-genome sequencing to be caused by the Omicron sublineages BA.1, BA.2, BA.4, or BA.5. These specimens were used to infect Vero E6 cells, derived from monkey kidneys, for the purpose of viral isolation. Viral stocks were then passaged in Vero E6 cells at a multiplicity of infection of 0.01, and culture supernatants were harvested at 12-hour intervals for 72 hours. To evaluate viral replication kinetics, we determined the cycle threshold values of the supernatants using real-time reverse transcription polymerase chain reaction and converted these values to genome copy numbers. Results: The viral load was comparable between BA.2, BA.4, and BA.5, whereas BA.1 exhibited a lower value. The peak infectious load of BA.4 was approximately 3 times lower than that of BA.2 and BA.5, while the peak load of BA.2 and BA.5 was about 7 times higher than that of BA.1. Notably, BA.1 demonstrated the lowest infectivity over the entire study period. Conclusion: Our results suggest that the global BA.5 wave may have been amplified by the higher viral replication and infectivity of BA.5 compared to other Omicron sublineages. These analyses could support the rapid assessment of the impact of novel variants on case incidence.

Citations

Citations to this article as recorded by  
  • 최근 코로나바이러스감염증-19 변이 바이러스 유행 현황 및 세포기반의 오미크론 세부계통 KP.3 감염성 분석
    정민 김, 진선 노, 동주 김, 지영 노, 채영 이, 상희 우, 남주 이, 지은 이, 일환 김, 은진 김
    Public Health Weekly Report.2024; 17(39): 1671.     CrossRef
Original Article
Increased viral load in patients infected with severe acute respiratory syndrome coronavirus 2 Omicron variant in the Republic of Korea
Jeong-Min Kim, Dongju Kim, Nam-Joo Lee, Sang Hee Woo, Jaehee Lee, Hyeokjin Lee, Ae Kyung Park, Jeong-Ah Kim, Chae Young Lee, Il-Hwan Kim, Cheon Kwon Yoo, Eun-Jin Kim
Osong Public Health Res Perspect. 2023;14(4):272-278.   Published online July 27, 2023
DOI: https://doi.org/10.24171/j.phrp.2023.0024
  • 2,071 View
  • 119 Download
  • 2 Web of Science
  • 2 Crossref
Graphical AbstractGraphical Abstract AbstractAbstract PDF
Objectives
Coronavirus disease 2019 (COVID-19) has been declared a global pandemic owing to the rapid spread of the causative agent, severe acute respiratory syndrome coronavirus 2. Its Delta and Omicron variants are more transmissible and pathogenic than other variants. Some debates have emerged on the mechanism of variants of concern. In the COVID-19 wave that began in December 2021, the Omicron variant, first reported in South Africa, became identifiable in most cases globally. The aim of this study was to provide data to inform effective responses to the transmission of the Omicron variant.
Methods
The Delta variant and the spike protein D614G mutant were compared with the Omicron variant. Viral loads from 5 days after symptom onset were compared using epidemiological data collected at the time of diagnosis.
Results
The Omicron variant exhibited a higher viral load than other variants, resulting in greater transmissibility within 5 days of symptom onset.
Conclusion
Future research should focus on vaccine efficacy against the Omicron variant and compare trends in disease severity associated with its high viral load.

Citations

Citations to this article as recorded by  
  • Replication kinetics and infectivity of SARS-CoV-2 Omicron variant sublineages recovered in the Republic of Korea
    Jeong-Min Kim, Dongju Kim, Jee Eun Rhee, Cheon Kwon Yoo, Eun-Jin Kim
    Osong Public Health and Research Perspectives.2024; 15(3): 260.     CrossRef
  • Diagnostic Accuracy of the Abbott BinaxNOW COVID‐19 Antigen Card Test, Puerto Rico
    Zachary J. Madewell, Chelsea G. Major, Nathan Graff, Cameron Adams, Dania M. Rodriguez, Tatiana Morales, Nicole A. Medina Lopes, Rafael Tosado, Liliana Sánchez‐González, Janice Perez‐Padilla, Hannah R. Volkman, Jorge Bertrán‐Pasarell, Diego Sainz de la Pe
    Influenza and Other Respiratory Viruses.2024;[Epub]     CrossRef

PHRP : Osong Public Health and Research Perspectives
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