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- Molecular Typing of
Mycobacterium intracellulare Using Pulsed-Field Gel Electrophoresis, Variable-Number Tandem-Repeat Analysis, Mycobacteria Interspersed Repetitive-Unit-Variable-Number Tandem Repeat Typing, and Multilocus Sequence Typing: Molecular Characterization and Comparison of Each Typing Methods - Semi Jeon, Nara Lim, Seungjik Kwon, Taesun Shim, Misun Park, Bum-Joon Kim, Seonghan Kim
- Osong Public Health Res Perspect. 2014;5(3):119-130. Published online June 30, 2014
- DOI: https://doi.org/10.1016/j.phrp.2014.04.003
- 3,074 View
- 16 Download
- 8 Crossref
-
Abstract
PDF - Objectives
Mycobacterium intracellulare is the major causative agent of nontuberculous mycobacteria-related pulmonary infections. The strain typing of M. intracellulare is important for the treatment and control of its infections. We compared the discrimination capacity and effective value of four different molecular typing methods.
Methods
Antibiotic susceptibility testing, hsp65 and rpoB sequencing, pulsed-field gel electrophoresis (PFGE), multilocus sequence typing (MLST), mycobacteria interspersed repetitive-unit-variable-number tandem-repeat analysis (MIRU-VNTR), and VNTR assay targeting 44 M. intracellulare isolates obtained from patients with pulmonary infections were performed.
Results
All the antibiotic susceptibility patterns had no association with the molecular and sequence types tested in this study; however, the molecular and sequence types were related with each other. PFGE gave best results for discriminatory capacity, followed by VNTR, MLST, and MIRU-VNTR.
Conclusion
The high discriminatory power of PFGE, VNTR, and MLST is enough for differentiating between reinfection and relapse, as well as for other molecular epidemiological usages. The MLST could be regarded as a representative classification method, because it showed the clearest relation with the sequence types. -
Citations
Citations to this article as recorded by
- Differential Genotyping of Mycobacterium avium Complex and Its Implications in Clinical and Environmental Epidemiology
Jeong-Ih Shin, Sung Jae Shin, Min-Kyoung Shin
Microorganisms.2020; 8(1): 98. CrossRef - A strategy based on Amplified Fragment Length Polymorphism (AFLP) for routine genotyping of nontuberculous mycobacteria at the clinical laboratory
Sara Blanco-Conde, Carolina González-Cortés, Ramiro López-Medrano, Juan José Palacios-Gutiérrez, Cristina Diez-Tascón, Teresa Nebreda-Mayoral, María Josefa Sierra-García, Octavio Miguel Rivero-Lezcano
Molecular Biology Reports.2020; 47(5): 3397. CrossRef - Comparative Evaluation of Band-Based Genotyping Methods for Mycobacterium intracellulare and Its Application for Epidemiological Analysis
Jeong-Ih Shin, Jong-Hun Ha, Dong-Hae Lee, Jeong-Gyu Choi, Kyu-Min Kim, Seung Jun Lee, Yi Yeong Jeong, Jong Deog Lee, Myunghwan Jung, Seung-Chul Baik, Woo Kon Lee, Hyung-Lyun Kang, Min-Kyoung Shin, Jung-Wan Yoo
Microorganisms.2020; 8(9): 1315. CrossRef - Pulsed Field Gel Electrophoresis: Past, present, and future
Lilia Lopez-Canovas, Maximo B. Martinez Benitez, Jose A. Herrera Isidron, Eduardo Flores Soto
Analytical Biochemistry.2019; 573: 17. CrossRef - Molecular typing of Mycobacterium kansasii using pulsed-field gel electrophoresis and a newly designed variable-number tandem repeat analysis
Zofia Bakuła, Anna Brzostek, Paulina Borówka, Anna Żaczek, Izabela Szulc-Kiełbik, Agata Podpora, Paweł Parniewski, Dominik Strapagiel, Jarosław Dziadek, Małgorzata Proboszcz, Jacek Bielecki, Jakko van Ingen, Tomasz Jagielski
Scientific Reports.2018;[Epub] CrossRef -
Mycobacterium paraintracellulare sp. nov., for the genotype INT-1 of Mycobacterium intracellulare
So-Young Lee, Byoung-Jun Kim, Hong Kim, Yu-Seop Won, Che Ok Jeon, Joseph Jeong, Seon Ho Lee, Ji-Hun Lim, Seung-Heon Lee, Chang Ki Kim, Yoon-Hoh Kook, Bum-Joon Kim
International Journal of Systematic and Evolution.2016; 66(8): 3132. CrossRef - Methodological and Clinical Aspects of the Molecular Epidemiology of Mycobacterium tuberculosis and Other Mycobacteria
Tomasz Jagielski, Alina Minias, Jakko van Ingen, Nalin Rastogi, Anna Brzostek, Anna Żaczek, Jarosław Dziadek
Clinical Microbiology Reviews.2016; 29(2): 239. CrossRef - Genetic diversity of clinical Mycobacterium avium subsp. hominissuis and Mycobacterium intracellulare isolates causing pulmonary diseases recovered from different geographical regions
Kazuya Ichikawa, Jakko van Ingen, Won-Jung Koh, Dirk Wagner, Max Salfinger, Takayuki Inagaki, Kei-ichi Uchiya, Taku Nakagawa, Kenji Ogawa, Kiyofumi Yamada, Tetsuya Yagi
Infection, Genetics and Evolution.2015; 36: 250. CrossRef
- Differential Genotyping of Mycobacterium avium Complex and Its Implications in Clinical and Environmental Epidemiology
- Multiplex Real-time Polymerase Chain Reaction Assays for Simultaneous Detection of
Vibrio cholerae ,Vibrio parahaemolyticus , andVibrio vulnificus - Jie Yeun Park, Semi Jeon, Jun Young Kim, Misun Park, Seonghan Kim
- Osong Public Health Res Perspect. 2013;4(3):133-139. Published online June 30, 2013
- DOI: https://doi.org/10.1016/j.phrp.2013.04.004
- 3,185 View
- 32 Download
- 19 Crossref
-
Abstract
PDF
- Objectives
A multiplex real-time polymerase chain reaction (RT-PCR) method was developed for the identification of three Vibrio species: Vibrio cholerae, Vibrio parahaemolyticus, and Vibrio vulnificus.
Methods
Specific primers and probes targeting the hlyA, tlh, and vvhA genes were selected and used for multiplex real-time PCR to confirm the identification of V. cholerae, V. parahaemolyticus, and V. vulnificus, respectively. This method was applied to screen Vibrio species from environmental samples and combining it with a culture-based method, its effectiveness was evaluated in comparison with culture-based methods alone.
Results
Specific PCR fragments were obtained from isolates belonging to the target species, indicating a high specificity of this multiplex real-time PCR. No cross-reactivity with the assay was observed between the tested bacteria. The sensitivity of the multiplex real-time PCR was found to have a lower limit of 104 colony-forming units/reaction for all three Vibrio species. The combination strategy raised the isolation ratio of all three Vibrio species 1.26- to 2.75-fold.
Conclusion
This assay provides a rapid, sensitive, and specific technique to detect these three Vibrio species in the environment. -
Citations
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Microbiological Research.2019; 222: 43. CrossRef - Application of digital PCR and next generation sequencing in the etiology investigation of a foodborne disease outbreak caused by Vibrio parahaemolyticus
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The American Journal of Tropical Medicine and Hygi.2018; 99(1): 168. CrossRef - Detection and differentiation of Vibrio parahaemolyticus by multiplexed real-time PCR
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Canadian Journal of Microbiology.2018; 64(11): 809. CrossRef - Development of a rapid PCR protocol to detect Vibrio parahaemolyticus in clams
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Article
- Multiplex Real-Time Polymerase Chain Reaction-Based Method for the Rapid Detection of
gyrA andparC Mutations in Quinolone-ResistantEscherichia coli andShigella spp. - Junyoung Kim, Semi Jeon, Hyungjun Kim, Misun Park, Soobok Kim, Seonghan Kim
- Osong Public Health Res Perspect. 2012;3(2):113-117. Published online June 30, 2012
- DOI: https://doi.org/10.1016/j.phrp.2012.04.004
- 3,057 View
- 15 Download
- 6 Crossref
-
Abstract
PDF
- Two real-time polymerase chain reaction assays were developed to detect mutations in codons 83 and 87 in gyrA and in codons 80 and 91 in parC, the main sites that causes quinolone resistance in pathogenic Escherichia coli and Shigella spp. isolates. These assays can be employed as a useful method for controlling infections caused by quinolone-resistant E coli and Shigella isolates.
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Citations
Citations to this article as recorded by- Various Techniques for Molecular and Rapid Detection of Infectious and
Epidemic Diseases
Mohamad Hesam Shahrajabian, Wenli Sun
Letters in Organic Chemistry.2023; 20(9): 779. CrossRef - Molecular detection and Frequency of Fluoroquinolone-Resistant Escherichia coli by Multiplex Allele Specific Polymerase Chain Reaction (MAS-PCR)
Noha Tharwat Abou El-Khier, Maysaa El Sayed Zaki
Egyptian Journal of Basic and Applied Sciences.2020; 7(1): 1. CrossRef - Identification of new DNA gyrase inhibitors based on bioactive compounds from streptomyces: structure-based virtual screening and molecular dynamics simulations approaches
Hourieh Kalhor, Solmaz Sadeghi, Mahya Marashiyan, Reyhaneh Kalhor, Sanaz Aghaei Gharehbolagh, Mohammad Reza Akbari Eidgahi, Hamzeh Rahimi
Journal of Biomolecular Structure and Dynamics.2020; 38(3): 791. CrossRef - Soft sweep development of resistance in Escherichia coli under fluoroquinolone stress
Xianxing Xie, Ruichen Lv, Chao Yang, Yajun Song, Yanfeng Yan, Yujun Cui, Ruifu Yang
Journal of Microbiology.2019; 57(12): 1056. CrossRef - Rapid Detection of Genomic Mutations in gyrA and parC Genes of Escherichia coli by Multiplex Allele Specific Polymerase Chain Reaction
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Journal of Clinical Laboratory Analysis.2016; 30(6): 947. CrossRef - New concepts in diagnostics for infectious diarrhea
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Mucosal Immunology.2013; 6(5): 876. CrossRef
- Various Techniques for Molecular and Rapid Detection of Infectious and
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