<sec>
<title>Objectives</title>
<p>The <italic>CYP2B6</italic> is one of the most polymorphic <italic>CYP</italic> genes in humans that has the potential to modify the pharmacological and toxicological responses to clinically important drugs such as antimalarial artemisinin and its derivatives. The aim of the study was to determine the frequency of <italic>CYP2B6</italic> polymorphisms in Timor malaria endemic area, East Nusa Tenggara, Indonesia where Artemisin-based Combination Therapy (ACT) has been used to treat uncomplicated malaria.</p></sec>
<sec>
<title>Methods</title>
<p>A total of 109 healthy subjects were participated in this study. <italic>CYP2B6*4, *6</italic> and <italic>*9</italic> polymorphisms were analyzed using PCR-RFLP to confirm the SNPs prevalence of 516G>T and 785A>G in exon 4 and 5.</p></sec>
<sec>
<title>Results</title>
<p>There were 96 subjects included in the analysis. In the exon 4 of <italic>CYP2B6</italic> 516G>T, the frequency of the T mutation was 37.5% (39/96), and the wildtype 27.1% (26/96). In the exon 5, <italic>CYP2B6</italic> 785A>G mutant was detected in 29.2% (28/96) of individuals, and the wildtype allele in 35.4% (34/96). The frequency of <italic>CYP2B6*9</italic> (516G>T), <italic>CYP2B6*4</italic> (785A>G) and <italic>CYP2B6*6</italic> (516G>T and 785A>G) were 40.6%, 29.2% and 22.9%, respectively. The prevalence of these <italic>CYP2B6</italic> gene polymorphisms in Timorian ethnic were higher than that in Malay, Han Chinese, Indian, and Egyptian populations.</p></sec>
<sec>
<title>Conclusion</title>
<p>The prevalence of these <italic>CYP2B6</italic> 516G>T and 785A>G polymorphisms in Timorian ethnic is higher than that in other populations. These polymorphisms may affect the metabolism of artemisinin and its derivatives.</p></sec>
Citations
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