<sec>
<title>Objectives</title>
<p>This study aimed to identify virulent and antimicrobial resistant genes in fecal <italic>E. coli</italic> in Mbouda, Cameroon.</p></sec>
<sec>
<title>Methods</title>
<p>A total of 599 fecal samples were collected from patients with enteric infections who were ≥ 20 years old. <italic>E. coli</italic> was isolated on the MacConkey agar and virulent genes were detected by multiplex/simplex PCR. Isolates in which ≥ 1 virulent gene was detected were subjected to antibiotic susceptibility testing. The resulting resistant isolates were subjected to PCR, followed by sequencing for resistant genes detection.</p></sec>
<sec>
<title>Results</title>
<p>There were 119 enterovirulent <italic>E. coli</italic> identified, amongst which 47.05% were atypical enteropathogenic <italic>E. coli</italic> (EPEC), 36.97% enterotoxigenic <italic>E. coli</italic>, 10.08% Shiga toxin producing <italic>E. coli</italic> (STEC) and 5.88% were enteroinvasive <italic>E. coli</italic> (EIEC). The occurrence of the <italic>eae</italic> gene (47.06%) was higher compared with <italic>CVD432</italic> (33.61%), <italic>aaic</italic> (13.45%), <italic>stx2</italic> (10.08%) and <italic>stx1</italic> (0.84%). High resistance rates were noted for ampicillin (94.64% EPEC, 91.67% STEC, 59.09% EAEC, and 57.14% EIEC) and sulfamethoxazole-trimethoprim (100% EPEC and 83.33% STEC, 81.82% EAEC and 71.43% EIEC). <italic>sul2</italic> (71.43%), <italic>tetB</italic> (64.71%), <italic>tetA</italic> (59.94%) and <italic>blaTEM</italic> (52.10%) were detected. A double mutation (S83L; D87N) was seen in <italic>gyrA</italic> and a single mutation (S80I) was observed in <italic>parC</italic>.</p></sec>
<sec>
<title>Conclusion</title>
<p>These findings suggested that measures should be taken to reduce the harm of <italic>E. coli</italic> to public health.</p></sec>
Citations
Citations to this article as recorded by
Anti-virulence activity and immunomodulation of Oreoch-1 against enteroinvasive Escherichia coli infection Francesca Palma, Rosa Giugliano, Annalisa Chianese, Roberta Della Marca, Alessandra Monti, Nunzianna Doti, Carla Zannella, Massimiliano Galdiero, Anna De Filippis Microbial Pathogenesis.2026; 211: 108253. CrossRef
Detection of blaTEM, blaOXA, blaCTX-M, and blaSHV genes of antibiotic resistance in diarrheagenic E. coli causing enteric infection in hypertensive patients at Laquintinie Hospital, Littoral Region of Cameroon Ornella Djiolieu Tsobeng, Armelle T. Mbaveng, Michael F. Kengne, Ballue S.T. Dadjo, Delano G.T. Fonjou, Victor Kuete Journal of Infection and Public Health.2025; 18(1): 102617. CrossRef
Antibiotic Resistance Profile of Enterovirulent E. coli Isolates Harboring Broad‐Spectrum Beta‐Lactamase Genes in Cancer Patients at the Laquintinie Hospital in Douala, Littoral Region, Cameroon Michael F. Kengne, Armelle T. Mbaveng, Wiliane J. T. Marbou, Ousenu Karimo, Ballue S. T. Dadjo, Delano G. T. Fonjou, Ornella D. Tsobeng, Victor Kuete, Mohamed Salah Abbassi International Journal of Microbiology.2025;[Epub] CrossRef
Role of gyrase A/B double mutations along with Qnr genes in development of higher ciprofloxacin resistance among pathogenic Escherichia coli and Klebsiella pneumoniae Lena Dhara, Anusri Tripathi Microbial Pathogenesis.2025; 208: 107946. CrossRef
Resistance Profiles and Virulence Factors of Enteric Escherichia coli in Chronic Kidney Disease Patients at Laquintinie Hospital in Douala, Cameroon Ballue Serges T. Dadjo, Armelle T. Mbaveng, Jean W. Marbou Takougoum, Ornella D. Tsobeng, Michael F. Kengne, Victor Kuete, Graciela Russomando International Journal of Microbiology.2025;[Epub] CrossRef
Antimicrobial resistance of Enterobacterales in Central Africa: a systematic review and meta-analysis Mathilde Garé, Cédric B. Chesnais, Jérémy T. Campillo, Marie-Paule Ngogang, Marie-Christine Fonkoua, Vincent Le Moing, Charlotte Boullé Communications Medicine.2025;[Epub] CrossRef
Multidrug Resistant Enteric Bacteria from Cancer Patients Admitted in Douala Laquintinie Hospital, Littoral Region of Cameroon Michael F. Kengne, Ornella D. Tsobeng, Ballue S. T. Dadjo, Victor Kuete, Armelle T. Mbaveng, Tingtao Chen Canadian Journal of Infectious Diseases and Medica.2024;[Epub] CrossRef
Molecular Epidemiology and AMR Perspective of Diarrhoeagenic Escherichia coli in Africa: A Systematic Review and Meta-analysis John Bosco Kalule, Linda A. Bester, Daniel L. Banda, Firehiwot Abera Derra, Chisomo Msefula, Anthony M. Smith, Abraham Ajayi, Happiness Kumburu, Geoffrey Kwenda, Kaunda Yamba, John Mwaba, Yasmina J. Fakim, Nyasha Sithole, Aquillah M. Kanzi, Patrick M. K. Journal of Epidemiology and Global Health.2024; 14(4): 1381. CrossRef
Molecular epidemiology of antimicrobial resistance in central africa: A systematic review Annicet-Clotaire Dikoumba, Richard Onanga, Laurette G. Mangouka, Larson Boundenga, Edgard-Brice Ngoungou, Sylvain Godreuil
Access Microbiology
.2023;[Epub] CrossRef
Diarrheic <i>Escherichia coli</i>: A Predominant Etiological Agent of Gastroenteritis, a Case Study in Douala, Cameroon Amandine Plidikoua, Olivier Ziem, Justice T. Ngom, Afsa Mamboune, Balbine Adande Clémence, Francioli Koro Koro, Rosalie Annie Ngono Ngane Journal of Biosciences and Medicines.2023; 11(06): 1. CrossRef
Methanol extract from the seeds of Persea americana displays antibacterial and wound healing activities in rat model Steve E. Ekom, Jean-De-Dieu Tamokou, Victor Kuete Journal of Ethnopharmacology.2022; 282: 114573. CrossRef
Characterization of diarrhoeagenic Escherichia coli with special reference to antimicrobial resistance isolated from hospitalized diarrhoeal patients in Kolkata (2012–2019), India Debjani Ghosh, Goutam Chowdhury, Prosenjit Samanta, Sreeja Shaw, Alok K. Deb, Mainak Bardhan, Asis Manna, Shin-ichi Miyoshi, Thandavarayan Ramamurthy, Shanta Dutta, Asish K. Mukhopadhyay Journal of Applied Microbiology.2022; 132(6): 4544. CrossRef
Antibiotic resistomes and their chemical residues in aquatic environments in Africa Aemere Ogunlaja, Olumuyiwa O. Ogunlaja, Olumide D. Olukanni, Gloria O. Taylor, Chidinma G. Olorunnisola, Victorien T. Dougnon, Wassiyath Mousse, Despo Fatta-Kassinos, Titus A.M. Msagati, Emmanuel I. Unuabonah Environmental Pollution.2022; 312: 119783. CrossRef
Antibacterial and Therapeutic Potentials of the Capsicum annuum Extract against Infected Wound in a Rat Model with Its Mechanisms of Antibacterial Action Steve Endeguele Ekom, Jean-De-Dieu Tamokou, Victor Kuete, Dorota Formanowicz BioMed Research International.2021;[Epub] CrossRef
First
Prev
