Osong Public Health and Research Perspectives

Original Article

Study on Seroprevalence and Leptospiral Antibody Distribution among High-risk Planters in Malaysia: J. Mohd Ridzuan, B.D. Aziah, W.M. Zahiruddin; Osong Public Health Res Perspect. 2016;7(3):168-171. Published online June 30, 2016; DOI: https://doi.org/10.1016/j.phrp.2016.04.006

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Objectives
To determine the leptospirosis seroprevalence and to identify the predominant infecting serovars among oil palm plantation workers.
Methods
The cross-sectional study involved 350 asymptomatic oil palm plantation workers in Melaka and Johor. A serological test using the microscopic agglutination test was conducted in the Institute of Medical Research with a cut-off titre for seropositivity of ≥1:100.
Results
The overall seroprevalence of leptospiral antibodies was 28.6%. The job category with the highest seroprevalence was the fruit collector with 59.2%. The predominant serovar identified was serovar Sarawak (Lepto 175) (62%).
Conclusion
A high seroprevalence of leptospiral antibodies was detected among oil palm plantation workers and specifically among fruit collectors. The predominant infecting serovar among the workers was serovar Sarawak (Lepto 175). The findings suggest that more studies are needed to determine the reasons for the high seroprevalence and the transmission and pathogenicity of the local serovar Sarawak (Lepto 175).

Citations

Citations to this article as recorded by

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Article

Application of the Microagglutination Test for Serologic Diagnosis of Human Brucellosis: Sang-Hee Park, Yoo-Hoon Lee, Hyuk Chu, Seon-Do Hwang, Kyu-Jam Hwang, Hee-Yeol Choi, Mi-Yeoun Park; Osong Public Health Res Perspect. 2012;3(1):19-23. Published online December 31, 2011; DOI: https://doi.org/10.1016/j.phrp.2012.01.003

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Abstract PDF

Objectives
Brucellosis is one of the most common zoonoses in the world, and occurs mainly in farmers, slaughterhouse workers, and veterinarians via direct or indirect contact with infected animals or their products. The clinical symptoms of human brucellosis are nonspecific, such as fever, headache, chills, and sweating. Diagnosis and treatment of brucellosis requires laboratory tests. Although the serum tube agglutination test (SAT) is the standardized gold method, it is laborious, time consuming, and requires a number of reagents. A microagglutination test (MAT) variant of the SAT or enzyme-linked immunosorbent assay (ELISA) is recommended for serological diagnoses. For the simple and rapid diagnosis of brucellosis, the MAT was standardized using samples for the SAT to define positive and negative categories, and we then compared the sensitivity and specificity of the MAT and ELISA.
Methods
Thirty SAT-positive sera and 60 SAT-negative sera were used in this study. Antibody titers of ≥1:160 were considered positive readings in both the SAT and MAT. Brucella abortus antigens and Brucella-positive control antiserum were used in the SAT and MAT. ELISAs of IgM and IgG were performed according to the manufacturers’ instructions.
Results
The titers of the MAT differed according to antigen concentration. The optimal concentration of B abortus antigen was determined to compare the sensitivity and specificity between the MAT and SAT. The sensitivity and specificity of the MAT were 93.3% and 96.7%, respectively, for IgG with reference to ELISA, and 96.7% and 98.3%, respectively, for IgM.
Conclusions
The optimal concentration of antigen for the MAT was 1:10. The MAT is less time consuming and requires less antigen and serum than the SAT. The results of the MAT showed good agreement with those of ELISA. The results of this study suggest that the MAT could be useful for diagnosis of brucellosis.

Citations

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