Skip Navigation
Skip to contents

PHRP : Osong Public Health and Research Perspectives

OPEN ACCESS
SEARCH
Search

Search

Page Path
HOME > Search
2 "agglutination test"
Filter
Filter
Article category
Keywords
Publication year
Authors
Original Article
Study on Seroprevalence and Leptospiral Antibody Distribution among High-risk Planters in Malaysia
J. Mohd Ridzuan, B.D. Aziah, W.M. Zahiruddin
Osong Public Health Res Perspect. 2016;7(3):168-171.   Published online June 30, 2016
DOI: https://doi.org/10.1016/j.phrp.2016.04.006
  • 2,620 View
  • 20 Download
  • 12 Crossref
AbstractAbstract PDF
Objectives
To determine the leptospirosis seroprevalence and to identify the predominant infecting serovars among oil palm plantation workers.
Methods
The cross-sectional study involved 350 asymptomatic oil palm plantation workers in Melaka and Johor. A serological test using the microscopic agglutination test was conducted in the Institute of Medical Research with a cut-off titre for seropositivity of ≥1:100.
Results
The overall seroprevalence of leptospiral antibodies was 28.6%. The job category with the highest seroprevalence was the fruit collector with 59.2%. The predominant serovar identified was serovar Sarawak (Lepto 175) (62%).
Conclusion
A high seroprevalence of leptospiral antibodies was detected among oil palm plantation workers and specifically among fruit collectors. The predominant infecting serovar among the workers was serovar Sarawak (Lepto 175). The findings suggest that more studies are needed to determine the reasons for the high seroprevalence and the transmission and pathogenicity of the local serovar Sarawak (Lepto 175).

Citations

Citations to this article as recorded by  
  • Leptospirosis in Vietnam
    N. K. Tokarevich, O. V. Blinova
    Russian Journal of Infection and Immunity.2022; 12(6): 1019.     CrossRef
  • Seroprevalence and serovar distribution of Leptospirosis among healthy people in Vietnam: Results from a multi-center study
    Van Dinh Tran, Le Thi Phuong Mai, Nguyen Thi Thu, Bui Kim Linh, Phan Dang Than, Nguyen Tu Quyet, Luu Phuong Dung, Tran Ngoc Phuong Mai, Nguyen Thi My Hanh, Hoang Hai, Tran Nhu Duong, Dang Duc Anh
    Clinical Epidemiology and Global Health.2021; 10: 100700.     CrossRef
  • Cross-sectional survey for sheep leptospirosis in the northeast region of Brazil
    José Dêvede da Silva, Maira Porto Viana, Lucas Gonzales Lima Pereira Calado, Ana Milena César Lima, Francisco Selmo Fernandes Alves, Raimundo Rizaldo Pinheiro, Diego Figueiredo da Costa, Glaucenyra Cecília Pinheiro da Silva, Sérgio Santos de Azevedo, Cleb
    Preventive Veterinary Medicine.2021; 197: 105525.     CrossRef
  • Seroprevalence of leptospirosis in an endemic mixed urban and semi-urban setting—A community-based study in the district of Colombo, Sri Lanka
    Senaka Rajapakse, Praveen N. Weeratunga, Krishan Balaji, Kyra Charmaine Ramchandani, Udani Savbhagya de Silva, Shenali Avishka Ranasinghe, Dinesh Gunarathne, Pasindu P. B. Wijerathne, Narmada Fernando, Shiroma M. Handunnetti, Sumadhya Deepika Fernando, Me
    PLOS Neglected Tropical Diseases.2020; 14(5): e0008309.     CrossRef
  • An historical view of the experimental leptospiral infection in ruminants
    Bruno Ribeiro Rocha, Gabriel Martins, Walter Lilenbaum
    Comparative Immunology, Microbiology and Infectiou.2020; 73: 101532.     CrossRef
  • Detection of Leptospira kmetyi at recreational areas in Peninsular Malaysia
    Aznida Mohamad Zaki, Rozita Hod, Nadia Aqilla Shamsusah, Zaleha Md Isa, Siti Khairani Bejo, Hani Kartini Agustar
    Environmental Monitoring and Assessment.2020;[Epub]     CrossRef
  • High incidence of asymptomatic leptospirosis among urban sanitation workers from Kota Kinabalu, Sabah, Malaysian Borneo
    Mohammad Saffree Jeffree, Daisuke Mori, Nur Athirah Yusof, Azman Bin Atil, Khamisah Awang Lukman, Rafidah Othman, Mohd Rohaizat Hassan, Lela Suut, Kamruddin Ahmed
    Scientific Reports.2020;[Epub]     CrossRef
  • Association of rodent-borne Leptospira spp. with urban environments in Malaysian Borneo
    Kim R. Blasdell, Serge Morand, David Perera, Cadhla Firth, Mathieu Picardeau
    PLOS Neglected Tropical Diseases.2019; 13(2): e0007141.     CrossRef
  • Seroprevalence of leptospiral antibodies among market workers and food handlers in the central state of Malaysia
    S. Samsudin, S. N. S. Sakinah, O. Malina, B. A. Norliza, M. A. Noh, A. Fairuz, T. Z. M. T. Jamaluddin, R. A. Hamat, W. M. Zahiruddin, S. Mohd Nazri, S. Sukeri, B. D. Aziah, I. Zawaha, A. W. Zainudin, N. A. Munirah, M. N. Desa, V. Neela, S. N. Masri
    Tropical Medicine & International Health.2018; 23(3): 327.     CrossRef
  • Seroprevalence and distribution of leptospirosis serovars among wet market workers in northeastern, Malaysia: a cross sectional study
    Mas Harithulfadhli Agus Ab Rahman, Suhaily Mohd Hairon, Rukman Awang Hamat, Tengku Zetty Maztura Tengku Jamaluddin, Mohd Nazri Shafei, Norazlin Idris, Malina Osman, Surianti Sukeri, Zainudin A. Wahab, Wan Mohd Zahiruddin Wan Mohammad, Zawaha Idris, Aziah
    BMC Infectious Diseases.2018;[Epub]     CrossRef
  • Chronic experimental genital leptospirosis with autochthonous Leptospira santarosai strains of serogroup Sejroe
    Bruno Ribeiro Rocha, Mário Balaro, Paulo Victor Pereira, Gabriel Martins, Walter Lilenbaum
    Small Ruminant Research.2018; 164: 28.     CrossRef
  • Occurrence of Leptospira Species from Rodents, Soil and Water from an Oil Palm Plantation in Northern Sarawak
    Lesley Maurice Bilung, Chai Fung Pui, Ahmad Syatir Tahar, Kasing Apun, Lela Su`ut, Yee Ling Chong, Jayasilan Mohd-Azlan
    Asian Journal of Animal and Veterinary Advances.2018; 13(4): 332.     CrossRef
Article
Application of the Microagglutination Test for Serologic Diagnosis of Human Brucellosis
Sang-Hee Park, Yoo-Hoon Lee, Hyuk Chu, Seon-Do Hwang, Kyu-Jam Hwang, Hee-Yeol Choi, Mi-Yeoun Park
Osong Public Health Res Perspect. 2012;3(1):19-23.   Published online December 31, 2011
DOI: https://doi.org/10.1016/j.phrp.2012.01.003
  • 2,815 View
  • 16 Download
  • 15 Crossref
AbstractAbstract PDF
Objectives
Brucellosis is one of the most common zoonoses in the world, and occurs mainly in farmers, slaughterhouse workers, and veterinarians via direct or indirect contact with infected animals or their products. The clinical symptoms of human brucellosis are nonspecific, such as fever, headache, chills, and sweating. Diagnosis and treatment of brucellosis requires laboratory tests. Although the serum tube agglutination test (SAT) is the standardized gold method, it is laborious, time consuming, and requires a number of reagents. A microagglutination test (MAT) variant of the SAT or enzyme-linked immunosorbent assay (ELISA) is recommended for serological diagnoses. For the simple and rapid diagnosis of brucellosis, the MAT was standardized using samples for the SAT to define positive and negative categories, and we then compared the sensitivity and specificity of the MAT and ELISA.
Methods
Thirty SAT-positive sera and 60 SAT-negative sera were used in this study. Antibody titers of ≥1:160 were considered positive readings in both the SAT and MAT. Brucella abortus antigens and Brucella-positive control antiserum were used in the SAT and MAT. ELISAs of IgM and IgG were performed according to the manufacturers’ instructions.
Results
The titers of the MAT differed according to antigen concentration. The optimal concentration of B abortus antigen was determined to compare the sensitivity and specificity between the MAT and SAT. The sensitivity and specificity of the MAT were 93.3% and 96.7%, respectively, for IgG with reference to ELISA, and 96.7% and 98.3%, respectively, for IgM.
Conclusions
The optimal concentration of antigen for the MAT was 1:10. The MAT is less time consuming and requires less antigen and serum than the SAT. The results of the MAT showed good agreement with those of ELISA. The results of this study suggest that the MAT could be useful for diagnosis of brucellosis.

Citations

Citations to this article as recorded by  
  • The Development of Diagnostic and Vaccine Strategies for Early Detection and Control of Human Brucellosis, Particularly in Endemic Areas
    Ayman Elbehiry, Musaad Aldubaib, Eman Marzouk, Adil Abalkhail, Abdulaziz M. Almuzaini, Mohammed Rawway, Ali Alghamdi, Abdullah Alqarni, Mohammed Aldawsari, Abdelmaged Draz
    Vaccines.2023; 11(3): 654.     CrossRef
  • New insights into the genetic predisposition of brucellosis and its effect on the gut and vaginal microbiota in goats
    Ahmed M. Sallam, Ibrahim Abou-souliman, Henry Reyer, Klaus Wimmers, Alaa Emara Rabee
    Scientific Reports.2023;[Epub]     CrossRef
  • Bovine brucellosis – a comprehensive review
    Sandip Kumar Khurana, Anju Sehrawat, Ruchi Tiwari, Minakshi Prasad, Baldev Gulati, Muhammad Zubair Shabbir, Rajesh Chhabra, Kumaragurubaran Karthik, Shailesh Kumar Patel, Mamta Pathak, Mohd. Iqbal Yatoo, Vivek Kumar Gupta, Kuldeep Dhama, Ranjit Sah, Wanpe
    Veterinary Quarterly.2021; 41(1): 61.     CrossRef
  • Microbiological Laboratory Diagnosis of Human Brucellosis: An Overview
    Giovanni Di Bonaventura, Silvia Angeletti, Andrea Ianni, Tommasangelo Petitti, Giovanni Gherardi
    Pathogens.2021; 10(12): 1623.     CrossRef
  • The spatiotemporal distribution of human brucellosis in mainland China from 2007-2016
    Peifeng Liang, Yuan Zhao, Jianhua Zhao, Dongfeng Pan, Zhongqin Guo
    BMC Infectious Diseases.2020;[Epub]     CrossRef
  • Brucellar spondylodiscitis: A case series with focus on histopathological features
    Soumaya Rammeh, Emna Romdhane, Hend Riahi, Meriem Ksentini, Mouna Chelli Bouaziz, Rahma Ayadi, Aida Berriche, Yosra Chebbi, Mohamed Fethi Ladeb
    Journal of Clinical Neuroscience.2020; 78: 360.     CrossRef
  • A case report of neurobrucellosis mimicking Guillain–Barré syndrome
    Parastoo Paydarnia, Soroush Moradi, Arman Habibi, Ladan Abbasian, Mojdeh Ghabaee
    Neurology, Psychiatry and Brain Research.2019; 31: 27.     CrossRef
  • A compact laser diode based photoacoustic spectral response technique to differentiate Brucellosis infected goat tissue from normal tissues
    A. Gorey, S. Shukla, J.G. Prasad, S. Verma, A. Sharma, S. Vasudevan
    Journal of Instrumentation.2019; 14(05): P05017.     CrossRef
  • Laboratory Diagnosis of Human Brucellosis
    Pablo Yagupsky, Pilar Morata, Juan D. Colmenero
    Clinical Microbiology Reviews.2019;[Epub]     CrossRef
  • Real-time PCR assays for diagnosing brucellar spondylitis using formalin-fixed paraffin-embedded tissues
    Man Li, Xingang Zhou, Jingjing Li, Lei Sun, Xiangmei Chen, Peng Wang
    Medicine.2018; 97(9): e0062.     CrossRef
  • Incidence of human brucellosis in the Kilimanjaro Region of Tanzania in the periods 2007–2008 and 2012–2014
    Manuela Carugati, Holly M Biggs, Michael J Maze, Robyn A Stoddard, Shama Cash-Goldwasser, Julian T Hertz, Jo E B Halliday, Wilbrod Saganda, Bingileki F Lwezaula, Rudovick R Kazwala, Sarah Cleaveland, Venance P Maro, Matthew P Rubach, John A Crump
    Transactions of The Royal Society of Tropical Medi.2018; 112(3): 136.     CrossRef
  • Seroreactivity and Risk Factors Associated with Human Brucellosis among Cattle Slaughterhouse Workers in South Korea
    Dilaram Acharya, Seon Hwang, Ji-Hyuk Park
    International Journal of Environmental Research an.2018; 15(11): 2396.     CrossRef
  • Outbreak of human brucellosis in Southern Brazil and historical review of data from 2009 to 2018
    Tamilly Silva Lemos, Juliana Clelia Cequinel, Tania Portela Costa, Amanda Boni Navarro, Andressa Sprada, Flávia Kazumi Shibata, Regina Gondolfo, Felipe Francisco Tuon, Stephen Baker
    PLOS Neglected Tropical Diseases.2018; 12(9): e0006770.     CrossRef
  • Brucellosis in Immunocompromised Hosts
    KA Al-Anazi, AM Al-Jasser
    Archives of Organ Transplantation.2016; 1(1): 001.     CrossRef
  • Evaluation and Selection of Multilocus Variable-Number Tandem-Repeat Analysis Primers for Genotyping Brucella abortus Biovar 1 Isolated from Human Patients
    Subok Lee, Kyu-Jam Hwang, Mi-Yeoun Park, Seon-Do Hwang, Hee-Youl Chai, Hyuk Chu, Sang-Hee Park
    Osong Public Health and Research Perspectives.2013; 4(5): 265.     CrossRef

PHRP : Osong Public Health and Research Perspectives