- Development of a Diagnostic Kit to Detect Cryptosporidium parvum and Giardia lamblia
-
Hyeng-Il Cheun, Byung-Suk Chung, Da-Won Ma, Bo-La Goo, Shin-Hyeong Cho, Mi-jung Ji, Won-Ja Lee
-
Osong Public Health Res Perspect. 2013;4(3):146-151. Published online June 30, 2013
-
DOI: https://doi.org/10.1016/j.phrp.2013.04.006
-
-
3,563
View
-
22
Download
-
7
Crossref
-
 Abstract
 PDF
- Objectives
This study aims to develop a high-sensitivity antibody diagnostic kit that will enable a rapid and accurate detection of Cryptospofidium parvum and Giardia lamblia in patients with diarrhea.
Methods
The cultivated C. parvum oocysts and G. lamblia cysts in each calf and dog were injected to mice to obtain antibodies, which were titrated. Spleen cells of the immunized mouse were separated and blended with myelomas to produce hybrid cell lines that form monoclonal antibodies. Using ELISA method, antibodies that specifically respond to C. parvum and G.lamblia were then selected. The cells were injected into the abdominal cavity of a BALB/c mouse to isolate hydrops abdominis containing high level of antibodies. The IgG antibody was purified using protein G gel.
Results
The detection limit of monoclonal antibodies for Cryptosporidium parvum and Giardia lamblia was 125 oocysts/mL and 1250 cysts/mL, respectively. In addition, during testing they did not show cross-reactivity to viruses (n = 15), bacteria (n =17), and parasites (n = 9).
Conclusion
The rapid diagnostic antibody kit developed in this study, which specifically responds to C. parvum and G. lamblia, will be useful in detecting and monitoring diarrheal infections.
-
Citations
Citations to this article as recorded by  - Comprehensive review of conventional and state-of-the-art detection methods of Cryptosporidium
George Luka, Ehsan Samiei, Nishat Tasnim, Arash Dalili, Homayoun Najjaran, Mina Hoorfar
Journal of Hazardous Materials.2022; 421: 126714. CrossRef - A review ofCryptosporidiumspp. and their detection in water
Eman M. Hassan, Banu Örmeci, Maria C. DeRosa, Brent R. Dixon, Syed A. Sattar, Asma Iqbal
Water Science and Technology.2021; 83(1): 1. CrossRef - Portable on-chip colorimetric biosensing platform integrated with a smartphone for label/PCR-free detection of Cryptosporidium RNA
George S. Luka, Ephraim Nowak, Quin Robert Toyata, Nishat Tasnim, Homayoun Najjaran, Mina Hoorfar
Scientific Reports.2021;[Epub] CrossRef - Nanomaterials‐based biosensors for detection of microorganisms and microbial toxins
Laura Sutarlie, Sian Yang Ow, Xiaodi Su
Biotechnology Journal.2017;[Epub] CrossRef - ACG Clinical Guideline: Diagnosis, Treatment, and Prevention of Acute Diarrheal Infections in Adults
Mark S Riddle, Herbert L DuPont, Bradley A Connor
American Journal of Gastroenterology.2016; 111(5): 602. CrossRef - Multiplex-Touchdown PCR to Simultaneously Detect Cryptosporidium parvum, Giardia lamblia, and Cyclospora cayetanensis, the Major Causes of Traveler’s Diarrhea
Ji-Hun Shin, Sang-Eun Lee, Tong Soo Kim, Da-Won Ma, Jong-Yil Chai, Eun-Hee Shin
The Korean Journal of Parasitology.2016; 54(5): 631. CrossRef - Advancing Cryptosporidium Diagnostics from Bench to Bedside
Raul V. Destura, Rohani B. Cena, Ma. Jowina H. Galarion, Coleen M. Pangilinan, Geraldine M. Arevalo, Ryan Oliver C. Alba, Joy Ann G. Petronio, Gielenny M. Salem, Brian Schwem, Jesus Emmanuel A. D. Sevilleja
Current Tropical Medicine Reports.2015; 2(3): 150. CrossRef
- The First Outbreak of Giardiasis with Drinking Water in Korea
-
Hyeng-Il Cheun, Cheon-Hyeon Kim, Shin-Hyeong Cho, Da-Won Ma, Bo-La Goo, Mun-Su Na, Seung-Ki Youn, Won-Ja Lee
-
Osong Public Health Res Perspect. 2013;4(2):89-92. Published online April 30, 2013
-
DOI: https://doi.org/10.1016/j.phrp.2013.03.003
-
-
2,888
View
-
20
Download
-
24
Crossref
-
Abstract
PDF
- Objectives
To identify the pathogen of the diarrhea outbreak in a village in Jeollabuk province in Korea in April 2010.
Methods
DNA extraction was performed from the 120 L of collected water, which was centrifuged at 10,000 x g for 30 min. PCR reactions were conducted in a total of 25 ul, which included PCR premix (GenDEPOT, Barker, TX, USA), 2 ul (∼100 ng) of extracted DNA, and 10 pmol of each primer.
Results
Nine people out of 25 had a symptom of abdominal pain accompanied by diarrhea after they used stored valley water in a water tank as a provisional water supply source without chlorine sterilization. Among them Giardia lamblia was detected in fecal samples of 7 people using the polymerase chain reaction method. Although G. lamblia was also detected from water provided by the provisional water supply system stored in the water tank and used as drinking water, it was not detected in the water tank itself. This water-borne outbreak is considered to have occurred when the provisional water supply tube was destroyed under a building construction and contaminated by G. lamblia, but its precise cause has not been clarified.
Conclusion
This outbreak resulting from G. lamblia is very meaningful as the first outbreak of an infection by a water-borne parasite in Korea.
-
Citations
Citations to this article as recorded by - Molecular detection and assemblage analysis of the intestinal protozoan Giardia duodenalis in wild boars in Korea
Haeseung Lee, Dongmi Kwak
Frontiers in Veterinary Science.2023;[Epub] CrossRef - A scoping review of risk factors and transmission routes associated with human giardiasis outbreaks in high-income settings
Sarah Krumrie, Paul Capewell, Alison Smith-Palmer, Dominic Mellor, Willie Weir, Claire L. Alexander
Current Research in Parasitology & Vector-Borne Di.2022; 2: 100084. CrossRef - Prevalence and molecular characterization of Giardia duodenalis in small ruminants of Shiraz, southwestern Iran: A zoonotic concern
Ali Asghari, Farzad Mahdavi, Laya Shamsi, Mohammad Hossein Motazedian, Qasem Asgari, Saeed Shahabi, Behnam Mohammadi-Ghalehbin, Alireza Sadrebazzaz
Comparative Immunology, Microbiology and Infectiou.2022; 86: 101819. CrossRef - Genotypic and Epidemiologic Profiles of Giardia duodenalis in Four Brazilian Biogeographic Regions
Deiviane A. Calegar, Beatriz C. Nunes, Kerla J. L. Monteiro, Polyanna A. A. Bacelar, Brenda B. C. Evangelista, Mayron M. Almeida, Jurecir Silva, Jéssica P. Santos, Márcio N. Boia, Lauren H. Jaeger, Filipe A. Carvalho-Costa
Microorganisms.2022; 10(5): 940. CrossRef - Molecular Identification of Parasitic Protozoa Sarcocystis in Water Samples
Živilė Strazdaitė-Žielienė, Agnė Baranauskaitė, Dalius Butkauskas, Elena Servienė, Petras Prakas
Veterinary Sciences.2022; 9(8): 412. CrossRef - Assessment microbial risks for the presence of Cryptosporidium spp. and Giardia spp. based on the surveillance of the water supply systems in Colombia, 2014–2018
Blanca Lisseth Guzmán Barragán, Marcelo de Souza Lauretto, Maria Tereza Pepe Razzolini, Adelaide Cássia Nardocci, Karen Vanessa Marimón Sibaja
Water Environment Research.2022;[Epub] CrossRef - A Giardiasis Case Diagnosed by Wright’s Stain and BioFire FilmArray Gastrointestinal Panel Assay Using Stool Sample
Kui Hyun Yoon
Laboratory Medicine Online.2022; 12(4): 315. CrossRef - Diarrhoea among Children Aged 5 Years and Microbial Drinking Water Quality Compliance: Trends Analysis Study in South Africa (2008–2018)
Mohora Feida Malebatja, Mpata Mathildah Mokgatle
International Journal of Environmental Research an.2022; 20(1): 598. CrossRef - DNA-based detection of Leptospira wolffii, Giardia intestinalis and Toxoplasma gondii in environmental feces of wild animals in Korea
Priyanka KUMARI, Kyung Yeon EO, Woo-Shin LEE, Junpei KIMURA, Naomichi YAMAMOTO
Journal of Veterinary Medical Science.2021; 83(5): 850. CrossRef - Multilocus Genotyping of Giardia duodenalis Occurring in Korean Native Calves
Sang-Ik Oh, Suk-Han Jung, Han-Kyoung Lee, Changyong Choe, Tai-Young Hur, Kyoung-Min So
Veterinary Sciences.2021; 8(7): 118. CrossRef - Development of a simple, rapid multiplex PCR tool kit by using the 16S rRNA gene for the identification of faecal and non-faecal coliforms in drinking water
A. Shiva Shanker, N. Rajesh, Pavan Kumar Pindi
Water Supply.2021; 21(7): 3319. CrossRef - Use of the bivalve Dreissena polymorpha as a biomonitoring tool to reflect the protozoan load in freshwater bodies
Elodie Géba, Dominique Aubert, Loïc Durand, Sandy Escotte, Stéphanie La Carbona, Catherine Cazeaux, Isabelle Bonnard, Fanny Bastien, Mélissa Palos Ladeiro, Jitender P. Dubey, Isabelle Villena, Alain Geffard, Aurélie Bigot-Clivot
Water Research.2020; 170: 115297. CrossRef - Multilocus genotyping of Giardia duodenalis from pigs in Korea
Haeseung Lee, ByeongYeal Jung, Jun-Sik Lim, Min-Goo Seo, Seung-Hun Lee, Kwang-Ho Choi, Mi-Hye Hwang, Tae-Hwan Kim, Oh-Deog Kwon, Dongmi Kwak
Parasitology International.2020; 78: 102154. CrossRef - Multilocus genotyping and risk factor analysis of Giardia duodenalis in dogs in Korea
Ha-Young Kim, Haeseung Lee, Seung-Hun Lee, Min-Goo Seo, Seonju Yi, Jong Wan Kim, Chung Hyun Kim, Yu-Ran Lee, ByungJae So, Oh-Deog Kwon, Dongmi Kwak
Acta Tropica.2019; 199: 105113. CrossRef - Molecular Prevalence and Genotypes of Cryptosporidium parvum and Giardia duodenalis in Patients with Acute Diarrhea in Korea, 2013-2016
Da-Won Ma, Myoung-Ro Lee, Sung-Hee Hong, Shin-Hyeong Cho, Sang-Eun Lee
The Korean Journal of Parasitology.2019; 57(5): 531. CrossRef - The effect of water source and soil supplementation on parasite contamination in organic vegetable gardens
Fernanda Pinto Ferreira, Eloiza Teles Caldart, Roberta Lemos Freire, Regina Mitsuka-Breganó, Felipe Machado de Freitas, Ana Carolina Miura, Marcelle Mareze, Felippe Danyel Cardoso Martins, Mariana Ragassi Urbano, Adilson Luiz Seifert, Italmar Teodorico Na
Revista Brasileira de Parasitologia Veterinária.2018;[Epub] CrossRef - Identification of zoonotic Giardia duodenalis in Korean native calves with normal feces
Yoon-Joo Lee, Du-Gyeong Han, Ji-Hyoung Ryu, Jeong-Byoung Chae, Joon-Seok Chae, Do-Hyeon Yu, Jinho Park, Bae-Keun Park, Hyeon-Cheol Kim, Kyoung-Seong Choi
Parasitology Research.2018; 117(6): 1969. CrossRef - Waterborne transmission of protozoan parasites: Review of worldwide outbreaks - An update 2011–2016
Artemis Efstratiou, Jerry E. Ongerth, Panagiotis Karanis
Water Research.2017; 114: 14. CrossRef - Prevalence and molecular characterisation of Giardia duodenalis in calves with diarrhoea
S. H. Lee, D. VanBik, H. Y. Kim, A. Cho, J. W. Kim, J. W. Byun, J. K. Oem, S. I. Oh, D. Kwak
Veterinary Record.2016; 178(25): 633. CrossRef - Occurrence of Cryptosporidium spp. and Giardia spp. in a public water-treatment system, Paraná, Southern Brazil
Jonatas Campos Almeida, Felippe Danyel Cardoso Martins, José Maurício Ferreira Neto, Maíra Moreira dos Santos, João Luis Garcia, Italmar Teodorico Navarro, Emília Kiyomi Kuroda, Roberta Lemos Freire
Revista Brasileira de Parasitologia Veterinária.2015; 24(3): 303. CrossRef - A Case of Colitis by <i>Giardia lamblia</i> Infection with Atypical Presentation
Soo Kyoung Choi, Youn Hee Lee, Jinhee Lee, Seon Ho Park, Mi Hee Kim, Ok-Ran Shin, Young-Seok Cho
The Korean Journal of Medicine.2015; 89(3): 312. CrossRef - On the track for an efficient detection of Escherichia coli in water: A review on PCR-based methods
Diana Mendes Silva, Lucília Domingues
Ecotoxicology and Environmental Safety.2015; 113: 400. CrossRef - Genotyping and subtyping Cryptosporidium parvum and Giardia duodenalis carried by flies on dairy farms in Henan, China
Zifang Zhao, Haiju Dong, Rongjun Wang, Wei Zhao, Gongyi Chen, Shouyi Li, Meng Qi, Sumei Zhang, Fuchun Jian, Jinfeng Zhao, Longxian Zhang, Haiyan Wang, Aiqin Liu
Parasites & Vectors.2014;[Epub] CrossRef - Consistency of direct microscopic examination and ELISA in detection of Giardia in stool specimen among children
Zohreh Torabi, Ali Niksirat, Saeideh Mazloomzadeh, Akefeh Ahmadiafshar
Asian Pacific Journal of Tropical Disease.2014; 4: S725. CrossRef
|
