Figure 1Registration procedure for hESC lines in Korea. KCDC runs the registry and the advisory committee reviews the submitted data for the registry.
Figure 2Characteristics of three hESC lines with abnormal karyotypes. (A) Normal karyotype of H9. (B) Abnormal karyotype (70, XXY,+12) of CHA-hES 16. (C) Abnormal karyotype (47, XX, t(2;4)(q11.2;p15.2),+5) of CHA-hES 19. (D) Abnormal karyotype (90, XXXX,-6,-18,add(20)) of CHA-hES 26. (E) The relative quantities of pluripotency marker genes were detected by real-time PCR using Taqman probe. CHA-hES 16 showed lower levels of expression of several pluripotency markers, whereas other abnormal lines showed normal expression patterns. H9 was used as a positive control; mock and human fibroblasts were used as negative controls.
TableSummary of analyses of hESC lines submitted in 2010
a
|
Names of hESC linesb
|
Year |
Submitted data
|
KCDC-analyzed data
|
Registered |
Karyotype |
STR |
Immuno-staining |
RT-PCR |
Mycoplasma |
EB or teratoma |
Copies of informed consents |
List of surplus embryos |
Karyotype |
STR |
Immuno-staining |
RT-PCR |
Mycoplasma |
1 |
SNUhES1
|
2001 |
46, XY or 47, XY,+12 |
√ |
+ |
+ |
− |
Teratoma |
|
|
|
√c
|
|
|
|
Yes |
2 |
SNUhES2
|
2001 |
46, XX |
√ |
+ |
+ |
− |
Teratoma |
|
|
|
√c
|
|
|
|
Yes |
3 |
SNUhES3
|
2001 |
46, XY |
√ |
+ |
+ |
− |
Teratoma |
|
|
|
√c
|
+c
|
+c
|
|
Yes |
4 |
SNUhES4
|
2003 |
46, XY |
√ |
+ |
+ |
− |
Teratoma |
|
|
|
√c
|
+c
|
+c
|
|
Yes |
5 |
SNUhES5
|
2004 |
47, XY,+16 |
√ |
+ |
+ |
− |
Teratoma |
|
|
|
√c
|
|
|
|
Yes |
6 |
SNUhES6
|
2004 |
46, XY |
√ |
+ |
+ |
− |
EB |
|
|
|
√c
|
|
|
|
Yes |
7 |
SNUhES7
|
2004 |
46, XY |
√ |
+ |
+ |
− |
Teratoma |
|
|
|
√c
|
|
|
|
Yes |
8 |
SNUhES10
|
2004 |
69, XXX |
√ |
+ |
+ |
− |
EB |
|
|
|
√c
|
|
|
|
Yes |
9 |
SNUhES11
|
2004 |
46, XY |
√ |
+ |
+ |
− |
Teratoma |
|
|
|
√c
|
+c
|
+c
|
|
Yes |
10 |
SNUhES12
|
2004 |
46, XX |
√ |
+ |
+ |
− |
EB |
|
|
|
√c
|
|
|
|
Yes |
11 |
SNUhES14
|
2004 |
46, XY |
√ |
+ |
+ |
− |
EB |
|
|
|
√c
|
|
|
|
Yes |
12 |
SNUhES16
|
2004 |
46, XY |
√ |
+ |
+ |
− |
Teratoma |
|
|
|
√c
|
+c
|
+c
|
|
Yes |
13 |
SNUhES18
|
2004 |
46, XY or 47, XY,+12 |
√ |
+ |
+ |
− |
Teratoma |
|
|
|
√c
|
|
|
|
Yes |
14 |
SNUhES19
|
2004 |
46, XY or 47, XY,+12 |
√ |
+ |
+ |
− |
Teratoma |
|
|
|
√c
|
|
|
|
Yes |
15 |
CHA-hES 3
|
2005 |
46, XY |
√ |
+ |
+ |
− |
Teratoma |
√ |
√ |
|
√c
|
+c
|
+c
|
|
Yes |
16 |
CHA-hES 4
|
2004 |
46, XY |
√ |
+ |
+ |
− |
Teratoma |
√ |
√ |
|
√c
|
+c
|
+c
|
|
Yes |
17 |
CHA-hES 5
|
2004 |
46, XY, inv(9) |
√ |
+ |
+ |
− |
Teratoma |
√ |
√ |
|
√c
|
|
|
|
Yes |
18 |
CHA-hES 6
|
2004 |
46, XX |
√ |
+ |
+ |
− |
Teratoma |
√ |
√ |
|
√c
|
|
|
|
Yes |
19 |
CHA-hES 7
|
2004 |
46, XX |
√ |
+ |
+ |
− |
Teratoma |
√ |
√ |
|
√c
|
|
|
|
Yes |
20 |
CHA-hES 8
|
2004 |
46, XX |
√ |
+ |
+ |
− |
Teratoma |
√ |
√ |
|
√c
|
|
|
|
Yes |
21 |
CHA-hES 9
|
2004 |
46, XX |
√ |
+ |
+ |
− |
Teratoma |
√ |
√ |
|
√c
|
+c
|
+c
|
|
Yes |
22 |
CHA-hES 10
|
2005 |
46, XY |
√ |
+ |
+ |
− |
Teratoma |
√ |
√ |
|
√c
|
|
|
|
Yes |
23 |
CHA-hES 11
|
2005 |
46, XY |
√ |
+ |
+ |
− |
Teratoma |
√ |
√ |
|
√c
|
|
|
|
Yes |
24 |
CHA-hES 12
|
2005 |
46, XY |
√ |
+ |
+ |
− |
Teratoma |
√ |
√ |
|
√c
|
|
|
|
Yes |
25 |
Miz-hES4
|
2003 |
46, XY |
√ |
+ |
+ |
|
EB |
|
|
|
√c
|
+c
|
+c
|
|
Yes |
26 |
CHA-hES 13
|
2007 |
46, XX |
√ |
+ |
+ |
− |
Teratoma |
√ |
√ |
46, XX |
√ |
|
+ |
−d
|
Yes |
27 |
CHA-hES 14
|
2007 |
46, XY |
√ |
+ |
+ |
− |
Teratoma |
√ |
√ |
46, XY |
√ |
|
+ |
−d
|
Yes |
28 |
CHA-hES 15
|
2007 |
46, XY |
√ |
+ |
+ |
− |
Teratoma |
√ |
√ |
46, XY |
√ |
|
+ |
− |
Yes |
29 |
CHA-hES 16
|
2007 |
71, XXY |
√ |
+ |
+ |
− |
|
√ |
√ |
70, XXY,+12 |
√ |
|
+ |
−d
|
Yes |
30 |
CHA-hES 17
|
2007 |
46, XY |
√ |
+ |
+ |
− |
Teratoma |
√ |
√ |
46, XY |
√ |
|
+ |
−d
|
Yes |
31 |
CHA-hES 19
|
2007 |
47, XX, der(2), -4,+5,+mar |
√ |
+ |
+ |
− |
|
√ |
√ |
47, XX, t(2;4) q(11.2;p15.2),+5 |
√ |
|
+ |
−d
|
Yes |
32 |
CHA-hES 20
|
2007 |
46, XY |
√ |
+ |
+ |
− |
Teratoma |
√ |
√ |
46, XYe
|
√ |
|
+ |
− |
Yes |
33 |
CHA-hES 21
|
2008 |
46, XX |
√ |
+ |
+ |
− |
Teratoma |
√ |
√ |
46, XX |
√ |
|
+ |
− |
Yes |
34 |
CHA-hES 23
|
2008 |
46, XX |
√ |
+ |
|
− |
Teratoma |
√ |
√ |
46, XX |
√ |
|
+ |
− |
Yes |
35 |
CHA-hES 24
|
2008 |
46, XY |
√ |
+ |
+ |
− |
Teratoma |
√ |
√ |
46, XY |
√ |
|
+ |
− |
Yes |
36 |
CHA-hES 26
|
2008 |
Tri- or tetraploidy |
√ |
+ |
|
− |
|
√ |
√ |
90, XXXX,-6, -18,add(20) |
√ |
|
+ |
− |
Yes |
37 |
SNUhES31
|
2009 |
46, XY |
√ |
+ |
+ |
|
EB |
√ |
√ |
46, XY |
√ |
|
+ |
−d
|
Yes |
38 |
CHA-hES 18
|
2007 |
46, XX |
√ |
+ |
+ |
− |
Teratoma |
√ |
√ |
46, XX |
√ |
|
+ |
− |
Yes |
39 |
CHA-hES 22
|
2008 |
46, XX |
√ |
+ |
+ |
− |
Teratoma |
√ |
√ |
46, XX |
√ |
|
+ |
− |
Yes |
40 |
CHA-hES 25
|
2008 |
46, XX |
√ |
+ |
|
− |
Teratoma |
√ |
√ |
46, XX |
√ |
|
+ |
− |
Yes |
41 |
CHA-hES M1
|
2008 |
46, XX |
√ |
+ |
+ |
− |
Teratoma |
√ |
√ |
46, XX |
√ |
|
+ |
− |
Yes |
42 |
CHA-hES R1
|
2008 |
46, XY |
√ |
+ |
+ |
− |
Teratoma |
√ |
√ |
46, XY |
√ |
|
+ |
− |
Yes |
43 |
CHA-hES R2
|
2008 |
46, XX |
√ |
+ |
+ |
− |
Teratoma |
√ |
√ |
46, XX |
√ |
|
+ |
− |
Yes |
44 |
CHA-hES R3
|
2008 |
46, XX |
√ |
+ |
+ |
− |
Teratoma |
√ |
√ |
46, XX |
√ |
|
+ |
− |
Yes |
45 |
CHA-hES R4
|
2008 |
46, XY |
√ |
+ |
+ |
− |
Teratoma |
√ |
√ |
46, XY |
√ |
|
+ |
− |
yes |
46 |
CHA-hES B1
|
2009 |
46, XY |
√ |
+ |
+ |
− |
Teratoma |
√ |
√ |
46, XY |
√ |
|
+ |
− |
Yes |
47 |
CHA-hES B2
|
2009 |
46, XX |
√ |
+ |
+ |
− |
Teratoma |
√ |
√ |
46, XX |
√ |
|
+ |
− |
Yes |
48 |
CHA-hES B3
|
2009 |
46, XY |
√ |
+ |
+ |
− |
Teratoma |
√ |
√ |
46, XY |
√ |
|
+ |
− |
Yes |
49 |
AMC-1 |
2006 |
46, XY |
|
+ |
+ |
|
Teratoma |
√ |
√ |
46, XY |
√f
|
|
+ |
− |
No |
50 |
JNU-hES-01
|
2009 |
46, XY |
√ |
+ |
+ |
|
Difh
|
√ |
√ |
46, XY |
√ |
|
+ |
+g
|
Yes |
51 |
JNU-hES-02
|
2009 |
47, XY,+12 |
√ |
+ |
+ |
|
Difh
|
√ |
√ |
46, XY |
√ |
|
+ |
+g
|
Yes |
52 |
MB01 |
2002 |
46, XY |
|
+ |
+ |
|
|
|
|
|
|
|
|
|
Under review |
53 |
MB06 |
2004 |
46, XY |
|
+ |
+ |
|
|
|
|
|
|
|
|
|
Under review |
54 |
Sooam-hES1 |
2003 |
46, XX |
√ |
+ |
+ |
− |
Teratoma |
|
|
|
|
|
|
|
No |
55 |
SNUhES32
|
2010 |
46, XY |
√ |
+ |
+ |
− |
EB |
√ |
√ |
46, XY |
√ |
|
+ |
− |
Yes |