Figure 1Isolation rates of Bacillus cereus by (A) year of isolation, (B) patient age, and (C) patient gender in Korea, 2012–2014.
Table 1Primers used to amplify the target genes
Target gene |
Sequence (5′ to 3′) |
Size (bp) |
Reference |
entFM
|
CAAAGACTTCGTAACAAAAGGTGGT |
290 |
Yang et al, 2005 [7] |
|
TGTTTACTCCGCCTTTTACAAACTT |
|
|
nheA
|
ATTACAGGGTTATTGGTTACAGCAGT |
475 |
Yang et al, 2005 [7] |
|
AATCTTGCTCCATACTCTCTTGGATGCT |
|
|
cytK2
|
CAATCCCTGGCGCTAGTGCA |
585 |
Guinebretirere et al, 2006 [12] |
|
GTGTAGCCTGGACGAAGTTGG |
|
|
hblC
|
CCTATCAATACTCTCGCAACACCAAT |
386 |
Yang et al, 2005 [7] |
|
TTTTCTTGATTCGTCATAGCCATTTCT |
|
|
bceT
|
AGCTTGGAGCGGAGCAGACTATGT |
701 |
Yang et al, 2005 [7] |
|
GTATTTCTTTCCCGCTTGCCTTTT |
|
|
cer
|
ATCATAAAGGTGCGAACAAGA |
188 |
Kim et al, 2010 [22] |
|
AAGATCAACCGAATGCAACTG |
|
|
Table 2Presence of enterotoxin genes in emetic and non-emetic Bacillus cereus
Target gene |
entFM*
|
nheA*
|
cytK2*
|
hblC*
|
bceT*
|
cer*
|
Total strains (n = 667) |
567 (85.0) |
524 (78.6) |
297 (44.5) |
244 (36.6) |
198 (29.7) |
135 (20.2) |
Non-emetic strains (n = 532) |
462 (86.8) |
444 (83.5) |
273 (51.3) |
228 (42.9) |
181 (34.0) |
0 (0) |
Emetic strains (n = 135) |
105 (77.8) |
80 (59.3) |
24 (17.8) |
16 (11.9) |
17 (12.6) |
135 (100) |
Table 3Enterotoxin gene profiles of non-emetic and emetic toxin strains
Strains |
Group |
entFM
|
nheA
|
cytK2
|
hblC
|
bceT
|
cer
|
Strains, n (%) |
Non-emetic strains (n = 532) |
A |
+ |
+ |
+ |
+ |
+ |
− |
77 (14.5) |
|
B |
+ |
+ |
+ |
+ |
− |
− |
71 (13.3) |
|
C |
+ |
+ |
+ |
− |
− |
− |
35 (6.6) |
|
D |
+ |
+ |
+ |
− |
+ |
− |
33 (6.2) |
|
E |
+ |
+ |
− |
+ |
+ |
− |
9 (1.7) |
|
F |
+ |
+ |
− |
− |
+ |
− |
20 (3.8) |
|
G |
+ |
+ |
− |
+ |
− |
− |
25 (4.7) |
|
H |
+ |
+ |
− |
− |
− |
− |
125 (23.5) |
|
I |
+ |
− |
+ |
+ |
+ |
− |
6 (1.1) |
|
J |
+ |
− |
+ |
− |
+ |
− |
10 (1.9) |
|
K |
+ |
− |
+ |
+ |
− |
− |
6 (1.1) |
|
L |
+ |
− |
+ |
− |
− |
− |
10 (1.9) |
|
M |
+ |
− |
− |
+ |
+ |
− |
1 (0.2) |
|
N |
+ |
− |
− |
− |
+ |
− |
7 (1.3) |
|
O |
+ |
− |
− |
+ |
− |
− |
5 (0.9) |
|
P |
+ |
− |
− |
− |
− |
− |
22 (4.1) |
|
Q |
− |
+ |
+ |
+ |
+ |
− |
3 (0.6) |
|
R |
− |
+ |
+ |
+ |
− |
− |
6 (1.1) |
|
S |
− |
+ |
+ |
− |
− |
− |
9 (1.7) |
|
T |
− |
+ |
− |
+ |
+ |
− |
2 (0.4) |
|
U |
− |
+ |
− |
− |
+ |
− |
1 (0.2) |
|
V |
− |
+ |
− |
+ |
− |
− |
3 (0.6) |
|
W |
− |
+ |
− |
− |
− |
− |
25 (4.7) |
|
X |
− |
− |
+ |
− |
+ |
− |
2 (0.4) |
|
Y |
− |
− |
+ |
+ |
− |
− |
1 (0.2) |
|
a |
− |
− |
+ |
− |
− |
− |
4 (0.8) |
|
b |
− |
− |
− |
+ |
+ |
− |
9 (1.7) |
|
c |
− |
− |
− |
+ |
− |
− |
4 (0.8) |
|
d |
− |
− |
− |
− |
+ |
− |
1 (0.2) |
Emetic strains (n = 135) |
A |
+ |
+ |
+ |
+ |
+ |
+ |
3 (2.2) |
|
B |
+ |
+ |
+ |
+ |
− |
+ |
4 (3.0) |
|
C |
+ |
+ |
+ |
− |
+ |
+ |
4 (3.0) |
|
D |
+ |
+ |
+ |
− |
− |
+ |
1 (0.7) |
|
E |
+ |
+ |
− |
+ |
− |
+ |
1 (0.7) |
|
F |
+ |
+ |
− |
− |
− |
+ |
56 (41.5) |
|
G |
+ |
+ |
− |
− |
+ |
+ |
4 (3.0) |
|
H |
+ |
− |
+ |
+ |
− |
+ |
4 (3.0) |
|
I |
+ |
− |
+ |
+ |
+ |
+ |
2 (1.5) |
|
J |
+ |
− |
+ |
− |
− |
+ |
1 (0.7) |
|
K |
+ |
− |
+ |
− |
+ |
+ |
1 (0.7) |
|
L |
+ |
− |
− |
+ |
+ |
+ |
1 (0.7) |
|
M |
+ |
− |
− |
− |
+ |
+ |
1 (0.7) |
|
N |
+ |
− |
− |
− |
− |
+ |
22 (16.3) |
|
O |
− |
+ |
+ |
− |
− |
+ |
1 (0.7) |
|
P |
− |
+ |
− |
+ |
− |
+ |
1 (0.7) |
|
Q |
− |
+ |
− |
− |
− |
+ |
5 (3.7) |
|
R |
− |
− |
+ |
− |
+ |
+ |
1 (0.7) |
|
S |
− |
− |
+ |
− |
− |
+ |
2 (1.5) |
|
T |
− |
− |
− |
− |
− |
+ |
20 (14.8) |