Figure 1Selection of optimal pair for anti-Cryptosporidium parvum by the detection limit test.
Figure 2Selection of optimal pair for anti-Giardia lamblia by the detection limit test.
Figure 3Test procedure for Cryptosporidium parvus and Giardia lamblia rapid test kit.
Table 1Comparison of imported detection kits and antibody developed in this study
|
CORIS BioConcept Crypto-Strip
|
CORIS BioConcept Giardia-Strip
|
Cryptosporidium antigen detection ELISA
|
Giardia lamblia antigen detection ELISA
|
Positive |
Negative |
Positive |
Negative |
Positive |
Negative |
Positive |
Negative |
Antibody developed in this study |
Positive |
8 |
0 |
9 |
0 |
8 |
0 |
9 |
0 |
Negative |
0 |
30 |
0 |
30 |
0 |
30 |
0 |
30 |
Table 2Results of cross-reactivity testing for Cryptosporidium parvum and Giardia lamblia antigen rapid kit against 15 viruses
Panel |
Culture |
Strain |
Concentration (mg/mL) |
Result |
Reference |
Virus |
Adenovirus 3 |
GB |
1 |
—
|
ATCC VR3 |
Adenovirus 6 |
Tonsil 99 |
1.2 |
—
|
ATCC VR6 |
Adenovirus 21 |
AV-1645/128 |
1 |
—
|
ATCC VR256 |
Cytomegalovirus |
Towne |
1.5 |
—
|
ATCC VR977 |
Echovirus 2 |
Cornelis |
1.3 |
—
|
ATCC VR1039 |
Echovirus 5 |
Noyce |
0.9 |
—
|
ATCC VR1043 |
Echovirus 11 |
Gregory |
1 |
—
|
ATCC VR41 |
Herpes simplex virus 1 |
F |
0.25 |
—
|
ATCC VR733 |
Herpes simplex virus 2 |
G |
0.115 |
—
|
ATCC VR734 |
Mumps virus |
Enders |
1.8 |
—
|
ATCC VR1379 |
Parainfluenza virus 1 |
Sendai |
1.5 |
—
|
ATCC VR105 |
Parainfluenza virus 2 |
CA/Greer |
3.9 |
—
|
ATCC VR92 |
Parainfluenza virus 3 |
C243 |
1.93 |
—
|
ATCC VR93 |
Respiratory syncytial virus |
A-2 |
1 |
—
|
ATCC VR1540 |
Respiratory syncytial virus |
Long |
0.42 |
—
|
ATCC VR26 |
Table 3Results of cross-reactivity testing for Cryptosporidium parvum and Giardia lamblia Ag rapid test kit against 17 different bacteria
Panel |
Culture |
Concentration (cells/mL) |
Result (dilution ratio 1:100) |
Reference |
Bacteria |
Bordetella pertussis
|
1 × 108
|
—
|
ATCC 8467 |
Enterococcus faecalis
|
1 × 108
|
—
|
ATCC 4079 |
Escherichia coli
|
1 × 108
|
—
|
ATCC 25922 |
Haemophilus influenzae
|
1 × 108
|
—
|
ATCC 8142 |
Klebsiella pneumoniae
|
1 × 108
|
—
|
ATCC 4208 |
Legionella pneumophila
|
1 × 108
|
—
|
ATCC 33153 |
Mycobacterium avium
|
1 × 108
|
—
|
ATCC 15769 |
Mycobacterium intracellulare
|
1 × 108
|
—
|
KTCC 9514 |
Mycobacterium tuberculosis
|
1 × 108
|
—
|
ATCC 27294 |
Mycoplasma pneumoniae
|
1 × 108
|
—
|
ATCC 15492 |
Neisseria gonorrhoeae
|
1 × 108
|
—
|
ATCC 31953 |
Neisseria meningitidis
|
1 × 108
|
—
|
ATCC 6250 |
Proteus vulgaris
|
1 × 108
|
—
|
ATCC 6361 |
Pseudomonas aeruginosa
|
1 × 108
|
—
|
ATCC 27853 |
Staphylococcus aureus
|
1 × 108
|
—
|
ATCC 29213 |
Streptococcus pneumoniae
|
1 × 108
|
—
|
ATCC 19615 |
Streptococcus pyogenes
|
1 × 108
|
—
|
ATCC 4012 |
Table 4Results of cross-reactivity testing for the Cryptosporidium parvum and Giardia lamblia Ag rapid test kit against nine different parasites
Panel |
Culture |
Concentration (parasite/μL) |
Result (dilution ratio 1:100) |
Reference |
Parasite |
Entamoeba histolytica
|
1 × 104
|
—
|
ATCC 30190 |
Microsporidia
|
1 × 104
|
—
|
ATCC 50040 |
Cyclospora cayetanensis
|
1 × 104
|
—
|
Positive sample in this study |
Plasmodium vivax
|
1 × 104
|
—
|
Positive sample in this study |
Plasmodium falciparum
|
1 × 104
|
—
|
Positive sample in this study |
Toxoplasma gondii
|
1 × 104
|
—
|
ATCC 50174 |
Trypanosoma cruzi
|
1 × 104
|
—
|
ATCC 50791 |
Leishmania donovani
|
1 × 104
|
—
|
ATCC 50127 |
Leishmania infantum
|
1 × 104
|
—
|
ATCC 50134 |