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Original Article
Proteomic Analysis of Intracellular and Membrane Proteins From Voriconazole-Resistant Candida glabrata
Jae Il Yoo, Hwa Su Kim, Chi Won Choi, Jung Sik Yoo, Jae Yon Yu, Yeong Seon Lee
Osong Public Health Res Perspect. 2013;4(6):293-300.   Published online December 31, 2013
DOI: https://doi.org/10.1016/j.phrp.2013.10.001
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AbstractAbstract PDF
Objectives
The proteomic analysis of voriconazole resistant Candida glabrata strain has not yet been investigated. In this study, differentially expressed proteins of intracellular and membrane fraction from voriconazole-susceptible, susceptible dose-dependent (S-DD), resistant C. glabrata strains were compared with each other and several proteins were identified.
Methods
The proteins of intracellular and membrane were isolated by disrupting cells with glass bead and centrifugation from voriconazole susceptible, S-DD, and resistant C. glabrata strains. The abundance of expressed proteins was compared using two-dimensional sodium dodecyl sulfate polyacrylamide gel electrophoresis and proteins showing continuous twofold or more increase or reduction of expression in resistant strains compared to susceptible and S-DD strain were analyzed by liquid chromatography/mass spectrometry-mass spectrometry method.
Results
Of 34 intracellular proteins, 15 proteins showed expression increase or reduction (twofold or more). The identified proteins included regulation, energy production, carbohydrate transport, amino acid transport, and various metabolism related proteins. The increase of expression of heat shock protein 70 was found. Among membrane proteins, 12, 31 proteins showed expression increase or decrease in the order of susceptible, S-DD, and resistant strains. This expression included carbohydrate metabolism, amino acid synthesis, and response to stress-related proteins. In membrane fractions, the change of expression of 10 heat shock proteins was observed, and 9 heat shock protein 70 (Hsp70) showed the reduction of expression.
Conclusion
The expression of Hsp70 protein in membrane fraction is related to voriconazole resistant C. glabrata strains.

Citations

Citations to this article as recorded by  
  • What ‘Omics can tell us about antifungal adaptation
    Gabriela Fior Ribeiro, Eszter Denes, Helen Heaney, Delma S Childers
    FEMS Yeast Research.2022;[Epub]     CrossRef
  • Effects of antifungal agents on the fungal proteome: informing on mechanisms of sensitivity and resistance
    Rebecca A. Owens, Sean Doyle
    Expert Review of Proteomics.2021; 18(3): 185.     CrossRef
  • HPLC-MS identification and expression of Candida drug-resistance proteins from African HIV-infected patients
    Pedro M D S Abrantes, Randall Fisher, Patrick J D Bouic, Carole P McArthur, Burtram C Fielding, Charlene W J Africa
    AIMS Microbiology.2021; 7(3): 320.     CrossRef

PHRP : Osong Public Health and Research Perspectives