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Original Article
Profiling of Virulence-associated Factors in Shigella Species Isolated from Acute Pediatric Diarrheal Samples in Tehran, Iran
Sajad Yaghoubi, Reza Ranjbar, Mohammad Mehdi Soltan Dallal, Somayeh Yasliani Fard, Mohammad Hasan Shirazi, Mahmood Mahmoudi
Osong Public Health Res Perspect. 2017;8(3):220-226.   Published online June 30, 2017
DOI: https://doi.org/10.24171/j.phrp.2017.8.3.09
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  • 19 Crossref
AbstractAbstract PDF
Objectives

The genus Shigella comprises the most infectious and diarrheagenic bacteria causing severe diseases, mostly in children under five years of age. This study aimed to detect nine virulence genes (ipaBCD, VirA, sen, set1A, set1B, ial, ipaH, stx, and sat) in Shigella species (spp.) using multiplex polymerase chain reaction (MPCR) and to determine the relation of Shigella spp. from pediatric diarrheal samples with hospitalization and bloody diarrhea in Tehran, Iran.

Methods

Shigella spp. were isolated and identified using standard microbiological and serological methods. The virulence genes were detected using MPCR.

Results

Seventy-five Shigella spp. (40 S. sonnei, 33 S. flexneri, 1 S. dysenteriae, and 1 S. boydii) were isolated in this study. The prevalence of ial, sen, sat, set1A, and set1B was 74.7%, 45.4%, 28%, 24%, and 24%, respectively. All S. flexneri isolates, while no S. sonnei, S. dysenteriae, or S. boydii isolates, contained sat, set1A, and set1B. All isolates were positive for ipaH, ipaBCD, and virA, while one (1.4%) of the isolates contained stx. The highest prevalence of virulence determinants was found in S. flexneri serotype IIa. Nineteen (57.6%) of 33 S. flexneri isolates were positive for ipaBCD, ipaH, virA, ial, and sat. The sen determinants were found to be statistically significantly associated with hospitalization and bloody diarrhea (p = 0.001).

Conclusion

This study revealed a high prevalence of enterotoxin genes in S. flexneri, especially in serotype 2a, and has presented relations between a few clinical features of shigellosis and numerous virulence determinants of clinical isolates of Shigella spp.

Citations

Citations to this article as recorded by  
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  • Phenotypic and Genotypic Antibiotic Resistance Patterns of Clinical Shigella Isolates from Tehran, Iran
    Saeede Tahmasbi, Akram Sadat Tabatabaee Bafroee, Babak Pakbin
    Jundishapur Journal of Microbiology.2024;[Epub]     CrossRef
  • Antimicrobial susceptibility and virulence gene analysis of Shigella species causing dysentery in Iranian children: Implications for fluroquinolone resistance
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    Frontiers in Microbiology.2022;[Epub]     CrossRef
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    Molecular Biology Reports.2021; 48(2): 1645.     CrossRef
  • Evaluate the distribution of virulence genes and to investigate antibiotic resistance pattern among Shigella species isolated from children with shigellosis in Iran
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  • Burden, Antibiotic Resistance, and Clonality of Shigella spp. Implicated in Community-Acquired Acute Diarrhoea in Lilongwe, Malawi
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  • Frequency and Antimicrobial Resistance of Shigella Species in Iran During 2000-2020
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    Jundishapur Journal of Health Sciences.2021;[Epub]     CrossRef
  • Virulence factors and molecular characteristics of Shigella flexneri isolated from calves with diarrhea
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    BMC Microbiology.2021;[Epub]     CrossRef
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  • High Rates of Antimicrobial Resistance and Virulence Gene Distribution Among Shigella spp. Isolated from Pediatric Patients in Tehran, Iran


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    Annals of Clinical Microbiology and Antimicrobials.2020;[Epub]     CrossRef
  • Virulence-related genes are associated with clinical and nutritional outcomes of Shigella/Enteroinvasive Escherichia coli pathotype infection in children from Brazilian semiarid region: A community case-control study
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  • Genotyping and diversity of virulence genes among Shigella sonnei isolated from children with diarrhoea
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    Reviews in Medical Microbiology.2019; 30(4): 217.     CrossRef
  • Virulence gene profiles of Shigella species isolated from stool specimens in India: its association with clinical manifestation and antimicrobial resistance
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  • Prevalence of enterotoxin-encoding genes among diverse Shigella strains isolated from patients with diarrhea, southwest Iran
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  • Frequency of Mutations in Quinolone Resistance-Determining Regions and Plasmid-Mediated Quinolone Resistance in Shigella Isolates Recovered from Pediatric Patients in Tehran, Iran: An Overlooked Problem
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