<b>Objectives</b><br/> <i>Candida glabrata</i> is one of the most common causes of <i>Candida</i> bloodstream infections worldwide. Some isolates of <i>C glabrata </i>may be intermediately resistant to azoles, with some strains developing resistance during therapy or prophylaxis with fluconazole. In this study, we used a proteomic approach to identify differentially expressed proteins between fluconazoleresistant and -susceptible strains.<br/><b>Methods</b><br/> Membrane and cellular proteins were extracted from fluconazolesusceptible and fluconazole-resistant <i>C glabrata </i>strains. Differentially expressed proteins were compared using two-dimensional sodium dodecyl sulfate polyacrylamide gel electrophoresis. Proteins with &gt;1.5-fold difference in expression were identified by liquid chromatography tandem mass spectrometry (LC-MS/MS).<br/><b>Results</b><br/> A total of 65 proteins were differentially expressed in the cellular and membrane fractions. Among the 39 cellular proteins, 11 were upregulated and 28 were downregulated in fluconazole-resistant strains in comparison with fluconazole-susceptible strains. In the membrane fraction, a total of 26 proteins were found, of which 19 were upregulated and seven were downregulated. A total of 31 proteins were identified by LC-MS/MS that are involved in glycolysis, carbohydrate transport, energy transfer, and other metabolic pathways. Heat shock proteins were identified in various spots.<br/><b>Conclusion</b><br/> Heat shock and stress response proteins were upregulated in the membrane fraction of the fluconazole-resistant <i>C glabrata </i>strain. Compared with susceptible strains, fluconazole-resistant strains showed increased expression of membrane proteins and decreased expression of cellular proteins.