Skip Navigation
Skip to contents

PHRP : Osong Public Health and Research Perspectives

OPEN ACCESS
SEARCH
Search

Search

Page Path
HOME > Search
5 "serum"
Filter
Filter
Article category
Keywords
Publication year
Authors
Original Articles
Instability of Plasma and Serum Progastrin-Releasing Peptide During Repeated Freezing and Thawing
Jae-Eun Lee, Jin-Hyun Lee, Maria Hong, Seul-Ki Park, Ji-In Yu, So-Youn Shin, Shine Young Kim
Osong Public Health Res Perspect. 2016;7(6):351-355.   Published online December 31, 2016
DOI: https://doi.org/10.1016/j.phrp.2016.11.004
  • 2,153 View
  • 19 Download
  • 2 Citations
AbstractAbstract PDF
Objectives
Progastrin-releasing peptide (proGRP) is a promising biomarker for small cell lung cancer. However, not much is known about how sample processing and storage conditions affect the stability of proGRP. Here, we examined the effects of repeated freeze–thaw cycles on the stability of proGRP in plasma and serum.
Methods
Concentrations of proGRP were measured in plasma and serum samples exposed to two, three, or four freeze–thaw cycles and these were compared with values of corresponding samples exposed to one cycle (baseline). We also performed the area under the receiver-operating-characteristic curve (AUC) analysis to determine whether the differences of proGRP concentrations between each paired plasma and serum sample (ΔproGRP) can be used for identifying the samples that have been exposed to multiple freeze–thaw cycles.
Results
Concentrations of proGRP gradually decreased in both plasma and serum samples with increasing numbers of freeze–thaw cycles. Reduction rates of proGRP concentrations were greater in serum than in plasma samples and serum proGRP levels declined with statistical significance (p < 0.001) up to 10.1% after four freeze–thaw cycles. The ΔproGRP measurement showed fair accuracy (AUC = 0.741) for identifying samples that had been through four freeze–thaw cycles. The sensitivity was 82.8% and specificity was 62.1% at an optimal cut-off point of > 4.9.
Conclusion
Our study shows that the stability of circulating proGRP is affected in both plasma and serum samples by repeated freezing and thawing. We also show that ΔproGRP could be used for identifying paired plasma and serum samples subjected to multiple freeze–thaw cycles.

Citations

Citations to this article as recorded by  
  • Effect of Repeated Freeze–Thaw Cycles on Influenza Virus Antibodies
    Alessandro Torelli, Elena Gianchecchi, Martina Monti, Pietro Piu, Irene Barneschi, Carolina Bonifazi, Rosa Coluccio, Luisa Ganfini, Luciano Michele La Magra, Silvia Marconi, Ginevra Marzucchi, Ramona Pace, Laura Palladino, Bernardo Biagi, Emanuele Montomo
    Vaccines.2021; 9(3): 267.     CrossRef
  • The influence of different blood samples treatment methods on pro-gastrin-releasing peptide
    Huiqin Jiang, Ling Luo, Kang Xiong, Chengwen He, Huaizhou Wang, Yanghua Qin
    Medicine.2019; 98(26): e16130.     CrossRef
Inorganic Phosphorus and Potassium Are Putative Indicators of Delayed Separation of Whole Blood
Jae-Eun Lee, Maria Hong, Seul-Ki Park, Ji-In Yu, Jin-Sook Wang, Haewon Shin, Jong-Wan Kim, Bok-Ghee Han, So-Youn Shin
Osong Public Health Res Perspect. 2016;7(2):90-95.   Published online April 30, 2016
DOI: https://doi.org/10.1016/j.phrp.2015.11.003
  • 1,859 View
  • 15 Download
  • 6 Citations
AbstractAbstract PDF
Objectives
The delayed separation of whole blood can influence the concentrations of circulating blood components, including metabolites and cytokines. The aim of this study was to determine whether clinical-biochemistry analytes can be used to assess the delayed separation of whole blood.
Methods
We investigated the plasma and serum concentrations of five clinical-biochemistry analytes and free hemoglobin when the centrifugation of whole blood stored at 4°C or room temperature was delayed for 4 hours, 6 hours, 24 hours, or 48 hours, and compared the values with those of matched samples that had been centrifuged within 2 hours after whole-blood collection.
Results
The inorganic phosphorus (IP) levels in the plasma and serum samples were elevated ≥ 1.5-fold when whole-blood centrifugation was delayed at room temperature for 48 hours. Furthermore, the IP levels in the plasma samples showed excellent assessment accuracy [area under the receiver-operating-characteristic curve (AUC) > 0.9] after a 48-hour delay in whole-blood separation, and high sensitivity (100%) and specificity (95%) at an optimal cutoff point. The IP levels in the serum samples also exhibited good assessment accuracy (AUC > 0.8), and high sensitivity (81%) and specificity (100%). The potassium (K+) levels were elevated 1.4-fold in the serum samples following a 48-hour delay in whole-blood separation. The K+ levels showed excellent assessment accuracy (AUC > 0.9) following a 48-hour delay in whole-blood separation, and high sensitivity (95%) and specificity (91%) at an optimal cutoff point.
Conclusion
Our study showed that the IP and K+ levels in the plasma or serum samples could be considered as putative indicators to determine whether whole-blood separation had been delayed for extended periods.

Citations

Citations to this article as recorded by  
  • Blood Plasma Quality Control by Plasma Glutathione Status
    Tamara Tomin, Natalie Bordag, Elmar Zügner, Abdullah Al-Baghdadi, Maximilian Schinagl, Ruth Birner-Gruenberger, Matthias Schittmayer
    Antioxidants.2021; 10(6): 864.     CrossRef
  • Identification of specific pre-analytical quality control markers in plasma and serum samples
    Luz Ruiz-Godoy, Virginia Enríquez-Cárcamo, Lourdes Suárez-Roa, María Lourdes Lopez-Castro, Abel Santamaría, Mario Orozco-Morales, Ana Laura Colín-González
    Analytical Methods.2019; 11(17): 2259.     CrossRef
  • Proteomics and Lipidomics in Inflammatory Bowel Disease Research: From Mechanistic Insights to Biomarker Identification
    Bjoern Titz, Raffaella Gadaleta, Giuseppe Lo Sasso, Ashraf Elamin, Kim Ekroos, Nikolai Ivanov, Manuel Peitsch, Julia Hoeng
    International Journal of Molecular Sciences.2018; 19(9): 2775.     CrossRef
  • Impact of Preanalytical Variations in Blood-Derived Biospecimens on Omics Studies: Toward Precision Biobanking?
    Jae-Eun Lee, Young-Youl Kim
    OMICS: A Journal of Integrative Biology.2017; 21(9): 499.     CrossRef
  • Effect of delayed centrifugation of whole blood on serum samples stability
    Massimo Daves, Vincenzo Roccaforte, Michele Giacomi, Monica Riva, Maria Leitner, Stefan Platzgummer, Gertraud Goetsch, Giuseppe Lippi
    La Rivista Italiana della Medicina di Laboratorio .2017; 13(1): 41.     CrossRef
  • Instability of Plasma and Serum Progastrin-Releasing Peptide During Repeated Freezing and Thawing
    Jae-Eun Lee, Jin-Hyun Lee, Maria Hong, Seul-Ki Park, Ji-In Yu, So-Youn Shin, Shine Young Kim
    Osong Public Health and Research Perspectives.2016; 7(6): 351.     CrossRef
Effect of Repeated Freezing and Thawing on Biomarker Stability in Plasma and Serum Samples
Jae-Eun Lee, Shine Young Kim, So-Youn Shin
Osong Public Health Res Perspect. 2015;6(6):357-362.   Published online December 31, 2015
DOI: https://doi.org/10.1016/j.phrp.2015.11.005
  • 2,005 View
  • 16 Download
  • 37 Citations
AbstractAbstract PDF
Objectives
The stability of circulating proteins can be affected by repeated freezing and thawing. The aim of our study was to identify the effect of repeated freezing and thawing on the plasma and serum concentrations of eight proteins [interferon-γ, interleukin (IL)-8, IL-15, IL-17A, matrix metalloproteinase (MMP)-7, tumor necrosis factor-α, vascular endothelial growth factor (VEGF), and VEGF receptor 2 (VEGF-R2)].
Methods
We assessed the concentration changes of these proteins in 30 plasma and serum samples subjected to three, four, or five freeze–thaw cycles, and compared these with the concentration changes in the samples that were subjected to two freeze–thaw cycles before analysis.
Results
Repeated freezing and thawing by up to five cycles did not modify the plasma and serum concentrations of interferon-γ, IL-8, and VEGF-R2, while levels of MMP-7, tumor necrosis factor-α, and VEGF were significantly changed in both plasma and serum samples. Moreover, MMP-7 and VEGF concentrations tended to increase with freeze–thaw cycles. They were more elevated in plasma samples (up to about 15%) than in serum samples (up to about 7%), suggesting that serum is the preferred sample type for the analysis of circulating proteins.
Conclusion
This is the first report on the effect of repeated freezing and thawing on plasma concentrations of MMP-7 and VEGF-R2. Our findings propose that researchers should consider the number of freeze–thaw cycles to select plasma or serum samples, depending on the type of analyte.

Citations

Citations to this article as recorded by  
  • Clinical Spectroscopy: Lost in Translation?
    James M. Cameron, Christopher Rinaldi, Samantha H. Rutherford, Alexandra Sala, Ashton G. Theakstone, Matthew J. Baker
    Applied Spectroscopy.2022; 76(4): 393.     CrossRef
  • Correlation of Repeat Measurements of 27 Candidate Protein Markers for Colorectal Cancer Screening Taken Three Years and Multiple Freeze–Thaw Cycles Apart
    Megha Bhardwaj, Petra Schrotz-King, Hermann Brenner
    Life.2022; 12(3): 359.     CrossRef
  • Development of quinone linked immunosorbent assay (QuLISA) based on using Folin’s reagent as a non-enzymatic tag: Application to analysis of food allergens
    Mahmoud El-Maghrabey, Yudai Sato, Fatema Kaladari, Naoya Kishikawa, Naotaka Kuroda
    Sensors and Actuators B: Chemical.2022; 368: 132167.     CrossRef
  • A systematic review and meta-analysis of preanalytical factors and methodological differences influencing the measurement of circulating vascular endothelial growth factor
    Ulrika Sjöbom, Anders K. Nilsson, Hanna Gyllensten, Ann Hellström, Chatarina Löfqvist, Angela J. Glading
    PLOS ONE.2022; 17(7): e0270232.     CrossRef
  • Cytokines: From Clinical Significance to Quantification
    Chao Liu, Dewei Chu, Kourosh Kalantar‐Zadeh, Jacob George, Howard A. Young, Guozhen Liu
    Advanced Science.2021; 8(15): 2004433.     CrossRef
  • Microheterogeneity and preanalytical stability of protein biomarkers of inflammation and renal function
    Jie Gao, Arve Ulvik, Adrian McCann, Per Magne Ueland, Klaus Meyer
    Talanta.2021; 223: 121774.     CrossRef
  • The effect of pre-analytical handling on the stability of fractalkine, monocyte chemoattractant protein 1 (MCP1), interleukin 6 and interleukin 8 in samples of human cerebrospinal fluid
    Winnie Charlotte Pedersen Mortensen, Laila Bendix, Hanne Irene Jensen, Claus Varnum, Lasse Enkebølle Rasmussen, Jørgen T. Lauridsen, Nis Borbye-Lorenzen, Kristin Skogstrand, Palle Toft, Henrik Bjarke Vaegter, Morten Rune Blichfeldt-Eckhardt
    Journal of Immunological Methods.2021; 494: 113057.     CrossRef
  • Standardizing salivary lactoferrin measurements to obtain a robust diagnostic biomarker for Alzheimer's disease
    Fernando Bartolome, Gorka Orive, Eva Carro
    Alzheimer's & Dementia: Diagnosis, Assessment & Di.2021;[Epub]     CrossRef
  • Biobanking for Neurodegenerative Diseases: Challenge for Translational Research and Data Privacy
    Emilia Giannella, Valentino Notarangelo, Caterina Motta, Giulia Sancesario
    The Neuroscientist.2021; : 107385842110366.     CrossRef
  • Pre-analytical considerations in biomarker research: focus on cardiovascular disease
    Elena Revuelta-López, Jaume Barallat, Adriana Cserkóová, Carolina Gálvez-Montón, Allan S. Jaffe, James L. Januzzi, Antoni Bayes-Genis
    Clinical Chemistry and Laboratory Medicine (CCLM).2021; 59(11): 1747.     CrossRef
  • Vaginal cytokine profile and microbiota before and after lubricant use compared with condomless vaginal sex: a preliminary observational study
    Susan Tuddenham, Christina A. Stennett, Richard A. Cone, Jacques Ravel, Andrew N. Macintyre, Khalil G. Ghanem, Xin He, Rebecca M. Brotman
    BMC Infectious Diseases.2021;[Epub]     CrossRef
  • Proximity Extension Assay in Combination with Next-Generation Sequencing for High-throughput Proteome-wide Analysis
    Lotta Wik, Niklas Nordberg, John Broberg, Johan Björkesten, Erika Assarsson, Sara Henriksson, Ida Grundberg, Erik Pettersson, Christina Westerberg, Elin Liljeroth, Adam Falck, Martin Lundberg
    Molecular & Cellular Proteomics.2021; 20: 100168.     CrossRef
  • Serum or plasma? An old question looking for new answers
    Mario Plebani, Giuseppe Banfi, Sergio Bernardini, Francesco Bondanini, Laura Conti, Romolo Dorizzi, Fulvio Enrico Ferrara, Rita Mancini, Tommaso Trenti
    Clinical Chemistry and Laboratory Medicine (CCLM).2020; 58(2): 178.     CrossRef
  • Association Between Intravenous Fluid Bolus and Biomarker Trajectory During Prehospital Care
    Emily B. Brant, Jason Kennedy, Christian Martin-Gill, Vanessa Jackson, Octavia M. Peck Palmer, Clifton W. Callaway, Jeremy M. Kahn, Donald M. Yealy, Derek C. Angus, Christopher W. Seymour
    Prehospital Emergency Care.2020; 24(2): 196.     CrossRef
  • Evaluating sample stability in the clinical laboratory with the help of linear and non-linear regression analysis
    Joachim K.W. Pum
    Clinical Chemistry and Laboratory Medicine (CCLM).2020; 58(2): 188.     CrossRef
  • Optimization of Saliva Collection and Immunochromatographic Detection of Salivary Pepsin for Point-of-Care Testing of Laryngopharyngeal Reflux
    Young Ju Lee, Jiyoon Kwon, Sanggyeong Shin, Young-Gyu Eun, Jae Ho Shin, Gi-Ja Lee
    Sensors.2020; 20(1): 325.     CrossRef
  • Stability of 12 T-helper cell-associated cytokines in human serum under different pre-analytical conditions
    Ulrik Stervbo, Sharon Bajda, Patrizia Wehler, Benjamin J. Rohn, Melanie Streichhahn, Sehriban Temizsoy, Eva Kohut, Toralf Roch, Richard Viebahn, Timm H. Westhoff, Nina Babel
    Cytokine.2020; 129: 155044.     CrossRef
  • Quinone-based antibody labeling reagent for enzyme-free chemiluminescent immunoassays. Application to avidin and biotinylated anti-rabbit IgG labeling
    Mahmoud El-Maghrabey, Naoya Kishikawa, Shiori Harada, Kaname Ohyama, Naotaka Kuroda
    Biosensors and Bioelectronics.2020; 160: 112215.     CrossRef
  • Standardized approaches for clinical sampling and endpoint ascertainment in tuberculous meningitis studies
    Ursula K Rohlwink, Felicia C Chow, Sean Wasserman, Sofiati Dian, Rachel PJ Lai, Lidya Chaidir, Raph L Hamers, Robert J Wilkinson, David R Boulware, Fiona V Cresswell, Arjan van Laarhoven
    Wellcome Open Research.2020; 4: 204.     CrossRef
  • Exploring pre-analytical factors for the optimisation of serum diagnostics: Progressing the clinical utility of ATR-FTIR spectroscopy
    James M. Cameron, Holly J. Butler, David J. Anderson, Loren Christie, Lily Confield, Katie E. Spalding, Duncan Finlayson, Stuart Murray, Zanib Panni, Christopher Rinaldi, Alexandra Sala, Ashton G. Theakstone, Matthew J. Baker
    Vibrational Spectroscopy.2020; 109: 103092.     CrossRef
  • QuantiFERON-TB Gold Plus with Chemiluminescence Immunoassay: Do We Need a Higher Cutoff?
    Francesco Bisognin, Giulia Lombardi, Maria Carla Re, Paola Dal Monte, Geoffrey A. Land
    Journal of Clinical Microbiology.2020;[Epub]     CrossRef
  • Stability of serum biochemical markers during standard long-term storage and with a single thawing
    V. A. Kozlova, V. A. Metelskaya, M. S. Pokrovskaya, I. A. Efimova, O. A. Litinskaya, V. A. Kutsenko, E. B. Yarovaya, S. A. Shalnova, O. M. Drapkina
    Cardiovascular Therapy and Prevention.2020; 19(6): 2736.     CrossRef
  • Development and application of a porcine specific ELISA for the quantification of soluble CD163
    J. Alex Pasternak, Daniel J. MacPhee, John C.S. Harding
    Veterinary Immunology and Immunopathology.2019; 210: 60.     CrossRef
  • Dynamics of circulating TNF during adalimumab treatment using a drug-tolerant TNF assay
    Lea C. Berkhout, Merel J. l’Ami, Jill Ruwaard, Margreet H. Hart, Pleuni Ooijevaar-de Heer, Karien Bloem, Michael T. Nurmohamed, Ronald F. van Vollenhoven, Maarten Boers, Daniel F. Alvarez, Catherine H. Smith, Gerrit J. Wolbink, Theo Rispens
    Science Translational Medicine.2019;[Epub]     CrossRef
  • Standardized approaches for clinical sampling and endpoint ascertainment in tuberculous meningitis studies
    Ursula K Rohlwink, Felicia C Chow, Sean Wasserman, Sofiati Dian, Rachel PJ Lai, Lidya Chaidir, Raph L Hamers, Robert J Wilkinson, David R Boulware, Fiona V Cresswell, Arjan van Laarhoven
    Wellcome Open Research.2019; 4: 204.     CrossRef
  • The Effect of Anticoagulants, Temperature, and Time on the Human Plasma Metabolome and Lipidome from Healthy Donors as Determined by Liquid Chromatography-Mass Spectrometry
    Manoj Khadka, Andrei Todor, Kristal M. Maner-Smith, Jennifer K. Colucci, ViLinh Tran, David A. Gaul, Evan J. Anderson, Muktha S. Natrajan, Nadine Rouphael, Mark J. Mulligan, Circe E. McDonald, Mehul Suthar, Shuzhao Li, Eric A. Ortlund
    Biomolecules.2019; 9(5): 200.     CrossRef
  • A caspase-6-cleaved fragment of Glial Fibrillary Acidic Protein as a potential serological biomarker of CNS injury after cardiac arrest
    Ditte S. Jonesco, Christian Hassager, Martin Frydland, Jesper Kjærgaard, Morten Karsdal, Kim Henriksen, Stephen D. Ginsberg
    PLOS ONE.2019; 14(11): e0224633.     CrossRef
  • Angiogenic Factor and Cytokine Analysis among Patients Treated with Adjuvant VEGFR TKIs in Resected Renal Cell Carcinoma
    Wenxin Xu, Maneka Puligandla, Judith Manola, Andrea J. Bullock, Daniel Tamasauskas, David F. McDermott, Michael B. Atkins, Naomi B. Haas, Keith Flaherty, Robert G. Uzzo, Janice P. Dutcher, Robert S. DiPaola, Rupal S. Bhatt
    Clinical Cancer Research.2019; 25(20): 6098.     CrossRef
  • Stability of important antibodies for kidney disease: pre-analytic methodological considerations
    Qiuxia Han, Songyan Li, Bo Fu, Dongwei Liu, Maoqing Wu, Xiaoli Yang, Guangyan Cai, Zhangsuo Liu, Xiangmei Chen, Hanyu Zhu
    PeerJ.2018; 6: e5178.     CrossRef
  • The association of angiogenic factors and chronic kidney disease
    Christopher E. Anderson, L. Lee Hamm, Gem Batuman, Damodar R. Kumbala, Chung-Shiuan Chen, Swapna G. Kallu, Ravi Siriki, Shilpa Gadde, Myra A. Kleinpeter, N. Kevin Krane, Eric E. Simon, Jiang He, Jing Chen
    BMC Nephrology.2018;[Epub]     CrossRef
  • Strong impact on plasma protein profiles by precentrifugation delay but not by repeated freeze-thaw cycles, as analyzed using multiplex proximity extension assays
    Qiujin Shen, Johan Björkesten, Joakim Galli, Daniel Ekman, John Broberg, Niklas Nordberg, Annika Tillander, Masood Kamali-Moghaddam, Gunnel Tybring, Ulf Landegren
    Clinical Chemistry and Laboratory Medicine (CCLM).2018; 56(4): 582.     CrossRef
  • Plasma-Derived Inflammatory Proteins Predict Oral Squamous Cell Carcinoma
    Kelly Yi Ping Liu, Xian Jun David Lu, Yuqi Sarah Zhu, Nhu Le, Hugh Kim, Catherine F. Poh
    Frontiers in Oncology.2018;[Epub]     CrossRef
  • Is vascular endothelial growth factor a useful biomarker in giant cell arteritis?
    Nicola Goodfellow, Julien Morlet, Surjeet Singh, Afsie Sabokbar, Andrew Hutchings, Vanshika Sharma, Jana Vaskova, Shauna Masters, Allahdad Zarei, Raashid Luqmani
    RMD Open.2017; 3(1): e000353.     CrossRef
  • Indices of immune function used by ecologists are mostly unaffected by repeated freeze-thaw cycles and methodological deviations
    Arne Hegemann, Sara Pardal, Kevin D. Matson
    Frontiers in Zoology.2017;[Epub]     CrossRef
  • The Enhanced Liver Fibrosis (ELF) Panel: Analyte Stability Under Common Sample Storage Conditions Used in Clinical Practice
    Oliver J Kennedy, Julie Parkes, Sudeep Tanwar, Paul M Trembling, William M Rosenberg
    The Journal of Applied Laboratory Medicine.2017; 1(6): 720.     CrossRef
  • Instability of Plasma and Serum Progastrin-Releasing Peptide During Repeated Freezing and Thawing
    Jae-Eun Lee, Jin-Hyun Lee, Maria Hong, Seul-Ki Park, Ji-In Yu, So-Youn Shin, Shine Young Kim
    Osong Public Health and Research Perspectives.2016; 7(6): 351.     CrossRef
  • Effects of Long-Term Storage Time and Original Sampling Month on Biobank Plasma Protein Concentrations
    Stefan Enroth, Göran Hallmans, Kjell Grankvist, Ulf Gyllensten
    EBioMedicine.2016; 12: 309.     CrossRef
Aging-related Changes in Mouse Serum Glycerophospholipid Profiles
Seungwoo Kim, Hyo-Soon Cheon, Jae-Chun Song, Sang-Moon Yun, Sang Ick Park, Jae-Pil Jeon
Osong Public Health Res Perspect. 2014;5(6):345-350.   Published online December 31, 2014
DOI: https://doi.org/10.1016/j.phrp.2014.10.002
  • 1,861 View
  • 17 Download
  • 20 Citations
AbstractAbstract PDF
Objectives
Metabolic dysfunction is a common hallmark of the aging process and aging-related pathogenesis. Blood metabolites have been used as biomarkers for many diseases, including cancers, complex chronic diseases, and neurodegenerative diseases.
Methods
In order to identify aging-related biomarkers from blood metabolites, we investigated the specific metabolite profiles of mouse sera from 4-month-old and 21-month-old mice by using a combined flow injection analysis–tandem mass spectrometry and liquid chromatography–tandem mass spectrometry.
Results
Among the 156 metabolites detected, serum levels of nine individual metabolites were found to vary with aging. Specifically, lysophosphatidylcholine (LPC) acyl (a) C24:0 levels in aged mice were decreased compared to that in young mice, whereas phosphatidylcholine (PC) acyl-alkyl (ae) C38:4, PC ae C40:4, and PC ae C42:1 levels were increased. Three classes of metabolites (amino acids, LPCs, and PCs) differed in intraclass correlation patterns of the individual metabolites between sera from young and aged mice. Additionally, the ratio of LPC a C24:0 to PC ae C38:4 was decreased in the aged mice, whereas the ratio of PC ae C40:4 to LPC a C24:0 was increased, supporting the aging-related metabolic changes of glycerophospholipids.
Conclusion
The ratios of the individual metabolites PC and LPC could serve as potential biomarkers for aging and aging-related diseases.

Citations

Citations to this article as recorded by  
  • Apolipoprotein E knockout may affect cognitive function in D-galactose-induced aging mice through the gut microbiota–brain axis
    Bowei Chen, Jian Yi, Yaqian Xu, Huiqiao Wen, Fengming Tian, Yingfei Liu, Lan Xiao, Lisong Li, Baiyan Liu
    Frontiers in Neuroscience.2022;[Epub]     CrossRef
  • Posterior subcapsular cataracts are a late effect after acute exposure to 0.5 Gy ionizing radiation in mice
    Sarah Kunze, Alexander Cecil, Cornelia Prehn, Gabriele Möller, Andreas Ohlmann, Gerhild Wildner, Stephan Thurau, Kristian Unger, Ute Rößler, Sabine M. Hölter, Soile Tapio, Florian Wagner, Andreas Beyerlein, Fabian Theis, Horst Zitzelsberger, Ulrike Kulka,
    International Journal of Radiation Biology.2021; 97(4): 529.     CrossRef
  • Noninvasive Analysis Using Data-Independent Acquisition Mass Spectrometry: New Epidermal Proteins That Reveal Sex Differences in the Aging Process
    Shirui Chen, Hui Zhang, Mengting Liu, Yaochi Wang, Cong Xin, Jing Ma, Xiaodong Zheng, Xuejun Zhang, Liangdan Sun, Sen Yang, Alexandros Georgakilas
    Oxidative Medicine and Cellular Longevity.2021; 2021: 1.     CrossRef
  • Phospholipases and Reactive Oxygen Species Derived Lipid Biomarkers in Healthy and Diseased Humans and Animals – A Focus on Lysophosphatidylcholine
    Kathrin M. Engel, Jürgen Schiller, Christina E. Galuska, Beate Fuchs
    Frontiers in Physiology.2021;[Epub]     CrossRef
  • Metabolomics Study of Isocaloric Different Dietary Patterns on the Life Span in Healthy Population
    Cong Zhang, Qingna Yan, Qiushuang Zhu, Jinxiao Liu, Yuanjie Dong, Yuqiao Li, Ruohua Wang, Xuanfeng Tang, Xinyi Lv, Xiaoqing Li, Yunjiang Cai, Yucun Niu
    Clinical Interventions in Aging.2021; Volume 16: 2111.     CrossRef
  • Increased plasma phosphatidylcholine/lysophosphatidylcholine ratios in patients with Parkinson's disease
    Jadranka Miletić Vukajlović, Dunja Drakulić, Snežana Pejić, Tihomir V. Ilić, Aleksandra Stefanović, Marijana Petković, Jürgen Schiller
    Rapid Communications in Mass Spectrometry.2020;[Epub]     CrossRef
  • Effects of lifelong exercise and aging on the blood metabolic fingerprint of rats
    Anastasia Tzimou, Dimitra Benaki, Stefanos Nikolaidis, Emmanuel Mikros, Ioannis Taitzoglou, Vassilis Mougios
    Biogerontology.2020; 21(5): 577.     CrossRef
  • Ginsenoside Rb1 retards aging process by regulating cell cycle, apoptotic pathway and metabolism of aging mice
    Shujie Yu, Hui Xia, Yanlei Guo, Xiaoxian Qian, Xiaojuan Zou, Huabing Yang, Mingzhu Yin, Hongtao Liu
    Journal of Ethnopharmacology.2020; 255: 112746.     CrossRef
  • Exercise-induced recovery of plasma lipids perturbed by ageing with nanoflow UHPLC-ESI-MS/MS
    Kang Uk Kim, Kyeong Jin Yoon, Suhong Park, Jong Cheol Lee, Hyo Youl Moon, Myeong Hee Moon
    Analytical and Bioanalytical Chemistry.2020; 412(28): 8003.     CrossRef
  • Mouse Age Matters: How Age Affects the Murine Plasma Metabolome
    Patrick Pann, Martin Hrabě de Angelis, Cornelia Prehn, Jerzy Adamski
    Metabolites.2020; 10(11): 472.     CrossRef
  • Effects of Aging, Long-Term and Lifelong Exercise on the Urinary Metabolic Footprint of Rats
    Anastasia Tzimou, Stefanos Nikolaidis, Olga Begou, Aikaterina Siopi, Olga Deda, Ioannis Taitzoglou, Georgios Theodoridis, Vassilis Mougios
    Metabolites.2020; 10(12): 481.     CrossRef
  • Trends of Food Supply and Nutrient Intake in South Korea Over the Past 30 Years
    JONG-GYU KIM, JOONG-SOON KIM, JEONG - GYOO KIM
    Current Research in Nutrition and Food Science Jou.2019; 7(1): 85.     CrossRef
  • Whole Blood Transcriptome Analysis for Lifelong Monitoring in Elite Sniffer Dogs Produced by Somatic Cell Nuclear Transfer
    Sun-A Ock, Inchul Choi, Gi-Sun Im, Jae Gyu Yoo
    Cellular Reprogramming.2019; 21(6): 301.     CrossRef
  • Retracted Article: High-throughput metabolomics identifies serum metabolic signatures in acute kidney injury using LC-MS combined with pattern recognition approach
    Hai-Hong Li, Jian-Liang Pan, Su Hui, Xiao-Wei Ma, Zhi-Long Wang, Hui-Xin Yao, Jun-Feng Wang, Hong Li
    RSC Advances.2018; 8(27): 14838.     CrossRef
  • A Metabolomics Profiling of Glaucoma Points to Mitochondrial Dysfunction, Senescence, and Polyamines Deficiency
    Stéphanie Leruez, Alexandre Marill, Thomas Bresson, Grégoire de Saint Martin, Adrien Buisset, Jeanne Muller, Lydie Tessier, Cédric Gadras, Christophe Verny, Philippe Gohier, Patrizia Amati-Bonneau, Guy Lenaers, Dominique Bonneau, Gilles Simard, Dan Milea,
    Investigative Opthalmology & Visual Science.2018; 59(11): 4355.     CrossRef
  • Lactobacillus acidophilus DDS-1 Modulates the Gut Microbiota and Improves Metabolic Profiles in Aging Mice
    Ravichandra Vemuri, Tanvi Shinde, Rohit Gundamaraju, Shakuntla Gondalia, Avinash Karpe, David Beale, Christopher Martoni, Rajaraman Eri
    Nutrients.2018; 10(9): 1255.     CrossRef
  • Role of kinase-coupled TRP channels in mineral homeostasis
    Vladimir Chubanov, Lorenz Mittermeier, Thomas Gudermann
    Pharmacology & Therapeutics.2018; 184: 159.     CrossRef
  • Sarcosine Is Uniquely Modulated by Aging and Dietary Restriction in Rodents and Humans
    Ryan O. Walters, Esperanza Arias, Antonio Diaz, Emmanuel S. Burgos, Fangxia Guan, Simoni Tiano, Kai Mao, Cara L. Green, Yungping Qiu, Hardik Shah, Donghai Wang, Adam D. Hudgins, Tahmineh Tabrizian, Valeria Tosti, David Shechter, Luigi Fontana, Irwin J. Ku
    Cell Reports.2018; 25(3): 663.     CrossRef
  • Mild TBI Results in a Long-Term Decrease in Circulating Phospholipids in a Mouse Model of Injury
    Tanja Emmerich, Laila Abdullah, Joseph Ojo, Benoit Mouzon, Thinh Nguyen, Gary S. Laco, Gogce Crynen, James E. Evans, Jon Reed, Michael Mullan, Fiona Crawford
    NeuroMolecular Medicine.2017; 19(1): 122.     CrossRef
  • Improved motor and cognitive performance with sodium nitrite supplementation is related to small metabolite signatures: a pilot trial in middle-aged and older adults
    Jamie N. Justice, Lawrence C. Johnson, Allison E. DeVan, Charmion Cruickshank-Quinn, Nichole Reisdorph, Candace J. Bassett, Trent D. Evans, Forrest A. Brooks, Nathan S. Bryan, Michel B. Chonchol, Tony Giordano, Matthew B. McQueen, Douglas R. Seals
    Aging.2015; 7(11): 1004.     CrossRef
Articles
Serum MicroRNA Expression Profiling in Mice Infected with Rabies Virus
Myung Guk Han, Jun-Sun Park, Cho Soon Lee, Young Eui Jeong, Jung Sang Ryou, Jung Eun Cho, Young Ran Ju, Kyoung-Ki Lee
Osong Public Health Res Perspect. 2011;2(3):186-191.   Published online December 31, 2011
DOI: https://doi.org/10.1016/j.phrp.2011.11.043
  • 2,079 View
  • 18 Download
  • 6 Citations
AbstractAbstract PDF
Objectives
Serum or plasma microRNAs (miRNAs) are potential biomarkers for the diagnosis for cancer and prenatal diseases. This study was conducted to investigate whether rabies virus causes a change in serum miRNA expression.
Methods
ICR mice were intramuscularly inoculated with rabies virus and were sacrificed weekly to collect serum and brain tissue for 4 weeks postinoculation. Mice were assigned to four groups based on the results of indirect immunofluorescent assays, enzyme-linked immunosorbent assay, and nested reverse transcription-polymerase chain reaction and the expression profiles of serum miRNAs were compared using a commercial mouse miRNA expression profiling assay.
Results
The expression levels of miRNAs changed significantly with the different stages of the disease. The expression level of 94 serum miRNAs in infected mice changed at least twofold. Seven microRNAs of them were significantly upregulated or downregulated in all infected mice regardless of disease status. The number of miRNAs with an expression level change decreased with the progression of the disease. In a hierarchical cluster analysis, infected mice clustered into a group separate from uninfected control mice.
Conclusions
Based on the relationship of miRNAs to gene expression regulation, miRNAs may be candidates for the study of viral pathogenesis and could have potential as biomarkers.

Citations

Citations to this article as recorded by  
  • Expression Profiling and Bioinformatics Analysis of CircRNA in Mice Brain Infected with Rabies Virus
    Wen Zhao, Jingyin Su, Ningning Wang, Naiyu Zhao, Shuo Su
    International Journal of Molecular Sciences.2021; 22(12): 6537.     CrossRef
  • Altered microRNA expression in COVID-19 patients enables identification of SARS-CoV-2 infection
    Ryan J. Farr, Christina L. Rootes, Louise C. Rowntree, Thi H. O. Nguyen, Luca Hensen, Lukasz Kedzierski, Allen C. Cheng, Katherine Kedzierska, Gough G. Au, Glenn A. Marsh, Seshadri S. Vasan, Chwan Hong Foo, Christopher Cowled, Cameron R. Stewart, Ron A. M
    PLOS Pathogens.2021; 17(7): e1009759.     CrossRef
  • Machine Learning Identifies Cellular and Exosomal MicroRNA Signatures of Lyssavirus Infection in Human Stem Cell-Derived Neurons
    Ryan J. Farr, Nathan Godde, Christopher Cowled, Vinod Sundaramoorthy, Diane Green, Cameron Stewart, John Bingham, Carmel M. O’Brien, Megan Dearnley
    Frontiers in Cellular and Infection Microbiology.2021;[Epub]     CrossRef
  • MicroRNA Biomarkers for Infectious Diseases: From Basic Research to Biosensing
    Leon Tribolet, Emily Kerr, Christopher Cowled, Andrew G. D. Bean, Cameron R. Stewart, Megan Dearnley, Ryan J. Farr
    Frontiers in Microbiology.2020;[Epub]     CrossRef
  • Circulating microRNA profiles of Hendra virus infection in horses
    Christopher Cowled, Chwan-Hong Foo, Celine Deffrasnes, Christina L. Rootes, David T. Williams, Deborah Middleton, Lin-Fa Wang, Andrew G. D. Bean, Cameron R. Stewart
    Scientific Reports.2017;[Epub]     CrossRef
  • Systems Biomedicine of Rabies Delineates the Affected Signaling Pathways
    Sadegh Azimzadeh Jamalkandi, Sayed-Hamidreza Mozhgani, Hamid Gholami Pourbadie, Mehdi Mirzaie, Farshid Noorbakhsh, Behrouz Vaziri, Alireza Gholami, Naser Ansari-Pour, Mohieddin Jafari
    Frontiers in Microbiology.2016;[Epub]     CrossRef

PHRP : Osong Public Health and Research Perspectives