Osong Public Health and Research Perspectives

Original Articles

Instability of Plasma and Serum Progastrin-Releasing Peptide During Repeated Freezing and Thawing: Jae-Eun Lee, Jin-Hyun Lee, Maria Hong, Seul-Ki Park, Ji-In Yu, So-Youn Shin, Shine Young Kim; Osong Public Health Res Perspect. 2016;7(6):351-355. Published online December 31, 2016; DOI: https://doi.org/10.1016/j.phrp.2016.11.004

4,080 View
26 Download
2 Crossref

Objectives
Progastrin-releasing peptide (proGRP) is a promising biomarker for small cell lung cancer. However, not much is known about how sample processing and storage conditions affect the stability of proGRP. Here, we examined the effects of repeated freeze–thaw cycles on the stability of proGRP in plasma and serum.
Methods
Concentrations of proGRP were measured in plasma and serum samples exposed to two, three, or four freeze–thaw cycles and these were compared with values of corresponding samples exposed to one cycle (baseline). We also performed the area under the receiver-operating-characteristic curve (AUC) analysis to determine whether the differences of proGRP concentrations between each paired plasma and serum sample (ΔproGRP) can be used for identifying the samples that have been exposed to multiple freeze–thaw cycles.
Results
Concentrations of proGRP gradually decreased in both plasma and serum samples with increasing numbers of freeze–thaw cycles. Reduction rates of proGRP concentrations were greater in serum than in plasma samples and serum proGRP levels declined with statistical significance (p < 0.001) up to 10.1% after four freeze–thaw cycles. The ΔproGRP measurement showed fair accuracy (AUC = 0.741) for identifying samples that had been through four freeze–thaw cycles. The sensitivity was 82.8% and specificity was 62.1% at an optimal cut-off point of > 4.9.
Conclusion
Our study shows that the stability of circulating proGRP is affected in both plasma and serum samples by repeated freezing and thawing. We also show that ΔproGRP could be used for identifying paired plasma and serum samples subjected to multiple freeze–thaw cycles.

Citations

Citations to this article as recorded by

Effect of Repeated Freeze–Thaw Cycles on Influenza Virus Antibodies
Alessandro Torelli, Elena Gianchecchi, Martina Monti, Pietro Piu, Irene Barneschi, Carolina Bonifazi, Rosa Coluccio, Luisa Ganfini, Luciano Michele La Magra, Silvia Marconi, Ginevra Marzucchi, Ramona Pace, Laura Palladino, Bernardo Biagi, Emanuele Montomo
Vaccines.2021; 9(3): 267. CrossRef
The influence of different blood samples treatment methods on pro-gastrin-releasing peptide
Huiqin Jiang, Ling Luo, Kang Xiong, Chengwen He, Huaizhou Wang, Yanghua Qin
Medicine.2019; 98(26): e16130. CrossRef

Inorganic Phosphorus and Potassium Are Putative Indicators of Delayed Separation of Whole Blood: Jae-Eun Lee, Maria Hong, Seul-Ki Park, Ji-In Yu, Jin-Sook Wang, Haewon Shin, Jong-Wan Kim, Bok-Ghee Han, So-Youn Shin; Osong Public Health Res Perspect. 2016;7(2):90-95. Published online April 30, 2016; DOI: https://doi.org/10.1016/j.phrp.2015.11.003

4,010 View
22 Download
9 Crossref

Abstract PDF

Objectives
The delayed separation of whole blood can influence the concentrations of circulating blood components, including metabolites and cytokines. The aim of this study was to determine whether clinical-biochemistry analytes can be used to assess the delayed separation of whole blood.
Methods
We investigated the plasma and serum concentrations of five clinical-biochemistry analytes and free hemoglobin when the centrifugation of whole blood stored at 4°C or room temperature was delayed for 4 hours, 6 hours, 24 hours, or 48 hours, and compared the values with those of matched samples that had been centrifuged within 2 hours after whole-blood collection.
Results
The inorganic phosphorus (IP) levels in the plasma and serum samples were elevated ≥ 1.5-fold when whole-blood centrifugation was delayed at room temperature for 48 hours. Furthermore, the IP levels in the plasma samples showed excellent assessment accuracy [area under the receiver-operating-characteristic curve (AUC) > 0.9] after a 48-hour delay in whole-blood separation, and high sensitivity (100%) and specificity (95%) at an optimal cutoff point. The IP levels in the serum samples also exhibited good assessment accuracy (AUC > 0.8), and high sensitivity (81%) and specificity (100%). The potassium (K⁺) levels were elevated 1.4-fold in the serum samples following a 48-hour delay in whole-blood separation. The K⁺ levels showed excellent assessment accuracy (AUC > 0.9) following a 48-hour delay in whole-blood separation, and high sensitivity (95%) and specificity (91%) at an optimal cutoff point.
Conclusion
Our study showed that the IP and K⁺ levels in the plasma or serum samples could be considered as putative indicators to determine whether whole-blood separation had been delayed for extended periods.

Citations

Citations to this article as recorded by

Assessing the stability of uncentrifuged serum and plasma analytes at various post-collection intervals
Atiqah Mokhsin, Poonaresi Subramaniam, Sivasooriar Sivaneson, Nelson Nheu, Gobhy Ramaloo, Azana S. Hanifah, Sumitha B. Mahathevan, Mohanaraja Nadarajah, Gayathiri Sampasivam, Aletza Mohd Ismail, Thuhairah Abdul Rahman
Journal of Laboratory Medicine.2025; 49(1): 21. CrossRef
Concordance in COVID-19 serology, bone mineralization, and inflammatory analytes between venous and self-collected capillary blood samples exposed to various pre-analytical conditions
Banafshe Hosseini, Harika Dasari, Anna Smyrnova, Claude Bourassa, Jing Leng, Christian Renaud, Francine M Ducharme
Annals of Clinical Biochemistry: International Jou.2023; 60(4): 259. CrossRef
Assessment of avian health status: suitability and constraints of the Zoetis VetScan VS2 blood analyser for ecological and evolutionary studies
Ye Xiong, Michael Tobler, Arne Hegemann, Dennis L. Hasselquist
Biology Open.2023;[Epub] CrossRef
Blood Plasma Quality Control by Plasma Glutathione Status
Tamara Tomin, Natalie Bordag, Elmar Zügner, Abdullah Al-Baghdadi, Maximilian Schinagl, Ruth Birner-Gruenberger, Matthias Schittmayer
Antioxidants.2021; 10(6): 864. CrossRef
Identification of specific pre-analytical quality control markers in plasma and serum samples
Luz Ruiz-Godoy, Virginia Enríquez-Cárcamo, Lourdes Suárez-Roa, María Lourdes Lopez-Castro, Abel Santamaría, Mario Orozco-Morales, Ana Laura Colín-González
Analytical Methods.2019; 11(17): 2259. CrossRef
Proteomics and Lipidomics in Inflammatory Bowel Disease Research: From Mechanistic Insights to Biomarker Identification
Bjoern Titz, Raffaella M. Gadaleta, Giuseppe Lo Sasso, Ashraf Elamin, Kim Ekroos, Nikolai V. Ivanov, Manuel C. Peitsch, Julia Hoeng
International Journal of Molecular Sciences.2018; 19(9): 2775. CrossRef
Impact of Preanalytical Variations in Blood-Derived Biospecimens on Omics Studies: Toward Precision Biobanking?
Jae-Eun Lee, Young-Youl Kim
OMICS: A Journal of Integrative Biology.2017; 21(9): 499. CrossRef
Effect of delayed centrifugation of whole blood on serum samples stability
Massimo Daves, Vincenzo Roccaforte, Michele Giacomi, Monica Riva, Maria Leitner, Stefan Platzgummer, Gertraud Goetsch, Giuseppe Lippi
La Rivista Italiana della Medicina di Laboratorio .2017; 13(1): 41. CrossRef
Instability of Plasma and Serum Progastrin-Releasing Peptide During Repeated Freezing and Thawing
Jae-Eun Lee, Jin-Hyun Lee, Maria Hong, Seul-Ki Park, Ji-In Yu, So-Youn Shin, Shine Young Kim
Osong Public Health and Research Perspectives.2016; 7(6): 351. CrossRef

Effect of Repeated Freezing and Thawing on Biomarker Stability in Plasma and Serum Samples: Jae-Eun Lee, Shine Young Kim, So-Youn Shin; Osong Public Health Res Perspect. 2015;6(6):357-362. Published online December 31, 2015; DOI: https://doi.org/10.1016/j.phrp.2015.11.005

4,659 View
32 Download
46 Crossref

Abstract PDF

Objectives
The stability of circulating proteins can be affected by repeated freezing and thawing. The aim of our study was to identify the effect of repeated freezing and thawing on the plasma and serum concentrations of eight proteins [interferon-γ, interleukin (IL)-8, IL-15, IL-17A, matrix metalloproteinase (MMP)-7, tumor necrosis factor-α, vascular endothelial growth factor (VEGF), and VEGF receptor 2 (VEGF-R2)].
Methods
We assessed the concentration changes of these proteins in 30 plasma and serum samples subjected to three, four, or five freeze–thaw cycles, and compared these with the concentration changes in the samples that were subjected to two freeze–thaw cycles before analysis.
Results
Repeated freezing and thawing by up to five cycles did not modify the plasma and serum concentrations of interferon-γ, IL-8, and VEGF-R2, while levels of MMP-7, tumor necrosis factor-α, and VEGF were significantly changed in both plasma and serum samples. Moreover, MMP-7 and VEGF concentrations tended to increase with freeze–thaw cycles. They were more elevated in plasma samples (up to about 15%) than in serum samples (up to about 7%), suggesting that serum is the preferred sample type for the analysis of circulating proteins.
Conclusion
This is the first report on the effect of repeated freezing and thawing on plasma concentrations of MMP-7 and VEGF-R2. Our findings propose that researchers should consider the number of freeze–thaw cycles to select plasma or serum samples, depending on the type of analyte.

Citations

Citations to this article as recorded by

Reliability, stability during long-term storage, and intra-individual variation of circulating levels of osteopontin, osteoprotegerin, vascular endothelial growth factor-A, and interleukin-17A
Tomoki Nakamizo, John Cologne, Takeshi Kishi, Tetsuya Takahashi, Mayumi Inoue, Hiroyuki Ryukaku, Tomonori Hayashi, Yoichiro Kusunoki, Saeko Fujiwara, Waka Ohishi
European Journal of Medical Research.2024;[Epub] CrossRef
Quantitative and qualitative analysis of stability for 16 serum immunoregulators over 50 freeze–thaw cycles
R. N. Chowdhury, A. Armato, E. Culver, L. Shteynman, C. Kurien, B. Cradin, F. Margolin, T. Nguyen, C. Cardona, N. Kabir, R. M. Garruto, J. K. Lum, K. Wander
American Journal of Human Biology.2024;[Epub] CrossRef
Dynamics of serum levels of TNF-α in a longitudinal follow-up study in 98 patients with juvenile idiopathic arthritis treated with anti-TNF-α biological drugs
N. Morozova, M. Zajc Avramovič, G. Markelj, N. Toplak, T. Avčin
Clinical Rheumatology.2024; 43(7): 2287. CrossRef
Sample processing time but not storage time affects complement activation markers C4a, C4d, C3a, iC3b, Bb, C5a, and sC5b-9 levels in EDTA-plasma of individuals at clinical high-risk for psychosis
Eleftheria Kodosaki, Colm Healy, Jonah F. Byrne, Melanie Föcking, Mary Cannon, Diana O. Perkins, David Cotter, Meike Heurich
Biomarkers in Neuropsychiatry.2024; 10: 100097. CrossRef
Host blood protein biomarkers to screen for tuberculosis disease: a systematic review and meta-analysis
Mary Gaeddert, Kerstin Glaser, Bih H. Chendi, Ayten Sultanli, Lisa Koeppel, Emily L. MacLean, Tobias Broger, Claudia M. Denkinger, Christine Y. Turenne
Journal of Clinical Microbiology.2024;[Epub] CrossRef
Biobanking for Neurodegenerative Diseases: Challenge for Translational Research and Data Privacy
Emilia Giannella, Valentino Notarangelo, Caterina Motta, Giulia Sancesario
The Neuroscientist.2023; 29(2): 190. CrossRef
Keep cool! Observed temperature variations at different process stages of the biobanking workflow – examples from the Leipzig medical biobank
Juliane Weikert, Angelina Mehrländer, Ronny Baber
Journal of Laboratory Medicine.2023; 47(2): 69. CrossRef
The Stability of the Anti-Müllerian Hormone in Serum and Plasma Samples under Various Preanalytical Conditions
Radana Vrzáková, Václav Šimánek, Ondřej Topolčan, Vladimír Vurm, David Slouka, Radek Kučera
Diagnostics.2023; 13(8): 1501. CrossRef
Differences in the inflammatory proteome of East African and Western European adults and associations with environmental and dietary factors
Godfrey S Temba, Nadira Vadaq, Vesla Kullaya, Tal Pecht, Paolo Lionetti, Duccio Cavalieri, Joachim L Schultze, Reginald Kavishe, Leo AB Joosten, Andre J van der Ven, Blandina T Mmbaga, Mihai G Netea, Quirijn de Mast
eLife.2023;[Epub] CrossRef
Influence of Freezing Temperature, Freezing Duration, and Repeated Freeze/Thaw Cycles on Electrophoretic Profiles in the White Stork (Ciconia ciconia)
Milan Thorel, Yannick Roman, Antoine Leclerc
Journal of Avian Medicine and Surgery.2023;[Epub] CrossRef
Clinical Spectroscopy: Lost in Translation?
James M. Cameron, Christopher Rinaldi, Samantha H. Rutherford, Alexandra Sala, Ashton G. Theakstone, Matthew J. Baker
Applied Spectroscopy.2022; 76(4): 393. CrossRef
Correlation of Repeat Measurements of 27 Candidate Protein Markers for Colorectal Cancer Screening Taken Three Years and Multiple Freeze–Thaw Cycles Apart
Megha Bhardwaj, Petra Schrotz-King, Hermann Brenner
Life.2022; 12(3): 359. CrossRef
Development of quinone linked immunosorbent assay (QuLISA) based on using Folin’s reagent as a non-enzymatic tag: Application to analysis of food allergens
Mahmoud El-Maghrabey, Yudai Sato, Fatema Kaladari, Naoya Kishikawa, Naotaka Kuroda
Sensors and Actuators B: Chemical.2022; 368: 132167. CrossRef
A systematic review and meta-analysis of preanalytical factors and methodological differences influencing the measurement of circulating vascular endothelial growth factor
Ulrika Sjöbom, Anders K. Nilsson, Hanna Gyllensten, Ann Hellström, Chatarina Löfqvist, Angela J. Glading
PLOS ONE.2022; 17(7): e0270232. CrossRef
Cytokines: From Clinical Significance to Quantification
Chao Liu, Dewei Chu, Kourosh Kalantar‐Zadeh, Jacob George, Howard A. Young, Guozhen Liu
Advanced Science.2021;[Epub] CrossRef
Microheterogeneity and preanalytical stability of protein biomarkers of inflammation and renal function
Jie Gao, Arve Ulvik, Adrian McCann, Per Magne Ueland, Klaus Meyer
Talanta.2021; 223: 121774. CrossRef
The effect of pre-analytical handling on the stability of fractalkine, monocyte chemoattractant protein 1 (MCP1), interleukin 6 and interleukin 8 in samples of human cerebrospinal fluid
Winnie Charlotte Pedersen Mortensen, Laila Bendix, Hanne Irene Jensen, Claus Varnum, Lasse Enkebølle Rasmussen, Jørgen T. Lauridsen, Nis Borbye-Lorenzen, Kristin Skogstrand, Palle Toft, Henrik Bjarke Vaegter, Morten Rune Blichfeldt-Eckhardt
Journal of Immunological Methods.2021; 494: 113057. CrossRef
Standardizing salivary lactoferrin measurements to obtain a robust diagnostic biomarker for Alzheimer's disease
Fernando Bartolome, Gorka Orive, Eva Carro
Alzheimer's & Dementia: Diagnosis, Assessment & Di.2021;[Epub] CrossRef
Pre-analytical considerations in biomarker research: focus on cardiovascular disease
Elena Revuelta-López, Jaume Barallat, Adriana Cserkóová, Carolina Gálvez-Montón, Allan S. Jaffe, James L. Januzzi, Antoni Bayes-Genis
Clinical Chemistry and Laboratory Medicine (CCLM).2021; 59(11): 1747. CrossRef
Vaginal cytokine profile and microbiota before and after lubricant use compared with condomless vaginal sex: a preliminary observational study
Susan Tuddenham, Christina A. Stennett, Richard A. Cone, Jacques Ravel, Andrew N. Macintyre, Khalil G. Ghanem, Xin He, Rebecca M. Brotman
BMC Infectious Diseases.2021;[Epub] CrossRef
Proximity Extension Assay in Combination with Next-Generation Sequencing for High-throughput Proteome-wide Analysis
Lotta Wik, Niklas Nordberg, John Broberg, Johan Björkesten, Erika Assarsson, Sara Henriksson, Ida Grundberg, Erik Pettersson, Christina Westerberg, Elin Liljeroth, Adam Falck, Martin Lundberg
Molecular & Cellular Proteomics.2021; 20: 100168. CrossRef
Serum or plasma? An old question looking for new answers
Mario Plebani, Giuseppe Banfi, Sergio Bernardini, Francesco Bondanini, Laura Conti, Romolo Dorizzi, Fulvio Enrico Ferrara, Rita Mancini, Tommaso Trenti
Clinical Chemistry and Laboratory Medicine (CCLM).2020; 58(2): 178. CrossRef
Association Between Intravenous Fluid Bolus and Biomarker Trajectory During Prehospital Care
Emily B. Brant, Jason Kennedy, Christian Martin-Gill, Vanessa Jackson, Octavia M. Peck Palmer, Clifton W. Callaway, Jeremy M. Kahn, Donald M. Yealy, Derek C. Angus, Christopher W. Seymour
Prehospital Emergency Care.2020; 24(2): 196. CrossRef
Evaluating sample stability in the clinical laboratory with the help of linear and non-linear regression analysis
Joachim K.W. Pum
Clinical Chemistry and Laboratory Medicine (CCLM).2020; 58(2): 188. CrossRef
Optimization of Saliva Collection and Immunochromatographic Detection of Salivary Pepsin for Point-of-Care Testing of Laryngopharyngeal Reflux
Young Ju Lee, Jiyoon Kwon, Sanggyeong Shin, Young-Gyu Eun, Jae Ho Shin, Gi-Ja Lee
Sensors.2020; 20(1): 325. CrossRef
Stability of 12 T-helper cell-associated cytokines in human serum under different pre-analytical conditions
Ulrik Stervbo, Sharon Bajda, Patrizia Wehler, Benjamin J. Rohn, Melanie Streichhahn, Sehriban Temizsoy, Eva Kohut, Toralf Roch, Richard Viebahn, Timm H. Westhoff, Nina Babel
Cytokine.2020; 129: 155044. CrossRef
Quinone-based antibody labeling reagent for enzyme-free chemiluminescent immunoassays. Application to avidin and biotinylated anti-rabbit IgG labeling
Mahmoud El-Maghrabey, Naoya Kishikawa, Shiori Harada, Kaname Ohyama, Naotaka Kuroda
Biosensors and Bioelectronics.2020; 160: 112215. CrossRef
Standardized approaches for clinical sampling and endpoint ascertainment in tuberculous meningitis studies
Ursula K Rohlwink, Felicia C Chow, Sean Wasserman, Sofiati Dian, Rachel PJ Lai, Lidya Chaidir, Raph L Hamers, Robert J Wilkinson, David R Boulware, Fiona V Cresswell, Arjan van Laarhoven
Wellcome Open Research.2020; 4: 204. CrossRef
Exploring pre-analytical factors for the optimisation of serum diagnostics: Progressing the clinical utility of ATR-FTIR spectroscopy
James M. Cameron, Holly J. Butler, David J. Anderson, Loren Christie, Lily Confield, Katie E. Spalding, Duncan Finlayson, Stuart Murray, Zanib Panni, Christopher Rinaldi, Alexandra Sala, Ashton G. Theakstone, Matthew J. Baker
Vibrational Spectroscopy.2020; 109: 103092. CrossRef
QuantiFERON-TB Gold Plus with Chemiluminescence Immunoassay: Do We Need a Higher Cutoff?
Francesco Bisognin, Giulia Lombardi, Maria Carla Re, Paola Dal Monte, Geoffrey A. Land
Journal of Clinical Microbiology.2020;[Epub] CrossRef
Stability of serum biochemical markers during standard long-term storage and with a single thawing
V. A. Kozlova, V. A. Metelskaya, M. S. Pokrovskaya, I. A. Efimova, O. A. Litinskaya, V. A. Kutsenko, E. B. Yarovaya, S. A. Shalnova, O. M. Drapkina
Cardiovascular Therapy and Prevention.2020; 19(6): 2736. CrossRef
Development and application of a porcine specific ELISA for the quantification of soluble CD163
J. Alex Pasternak, Daniel J. MacPhee, John C.S. Harding
Veterinary Immunology and Immunopathology.2019; 210: 60. CrossRef
Dynamics of circulating TNF during adalimumab treatment using a drug-tolerant TNF assay
Lea C. Berkhout, Merel J. l’Ami, Jill Ruwaard, Margreet H. Hart, Pleuni Ooijevaar-de Heer, Karien Bloem, Michael T. Nurmohamed, Ronald F. van Vollenhoven, Maarten Boers, Daniel F. Alvarez, Catherine H. Smith, Gerrit J. Wolbink, Theo Rispens
Science Translational Medicine.2019;[Epub] CrossRef
Standardized approaches for clinical sampling and endpoint ascertainment in tuberculous meningitis studies
Ursula K Rohlwink, Felicia C Chow, Sean Wasserman, Sofiati Dian, Rachel PJ Lai, Lidya Chaidir, Raph L Hamers, Robert J Wilkinson, David R Boulware, Fiona V Cresswell, Arjan van Laarhoven
Wellcome Open Research.2019; 4: 204. CrossRef
The Effect of Anticoagulants, Temperature, and Time on the Human Plasma Metabolome and Lipidome from Healthy Donors as Determined by Liquid Chromatography-Mass Spectrometry
Manoj Khadka, Andrei Todor, Kristal M. Maner-Smith, Jennifer K. Colucci, ViLinh Tran, David A. Gaul, Evan J. Anderson, Muktha S. Natrajan, Nadine Rouphael, Mark J. Mulligan, Circe E. McDonald, Mehul Suthar, Shuzhao Li, Eric A. Ortlund
Biomolecules.2019; 9(5): 200. CrossRef
A caspase-6-cleaved fragment of Glial Fibrillary Acidic Protein as a potential serological biomarker of CNS injury after cardiac arrest
Ditte S. Jonesco, Christian Hassager, Martin Frydland, Jesper Kjærgaard, Morten Karsdal, Kim Henriksen, Stephen D. Ginsberg
PLOS ONE.2019; 14(11): e0224633. CrossRef
Angiogenic Factor and Cytokine Analysis among Patients Treated with Adjuvant VEGFR TKIs in Resected Renal Cell Carcinoma
Wenxin Xu, Maneka Puligandla, Judith Manola, Andrea J. Bullock, Daniel Tamasauskas, David F. McDermott, Michael B. Atkins, Naomi B. Haas, Keith Flaherty, Robert G. Uzzo, Janice P. Dutcher, Robert S. DiPaola, Rupal S. Bhatt
Clinical Cancer Research.2019; 25(20): 6098. CrossRef
Stability of important antibodies for kidney disease: pre-analytic methodological considerations
Qiuxia Han, Songyan Li, Bo Fu, Dongwei Liu, Maoqing Wu, Xiaoli Yang, Guangyan Cai, Zhangsuo Liu, Xiangmei Chen, Hanyu Zhu
PeerJ.2018; 6: e5178. CrossRef
The association of angiogenic factors and chronic kidney disease
Christopher E. Anderson, L. Lee Hamm, Gem Batuman, Damodar R. Kumbala, Chung-Shiuan Chen, Swapna G. Kallu, Ravi Siriki, Shilpa Gadde, Myra A. Kleinpeter, N. Kevin Krane, Eric E. Simon, Jiang He, Jing Chen
BMC Nephrology.2018;[Epub] CrossRef
Strong impact on plasma protein profiles by precentrifugation delay but not by repeated freeze-thaw cycles, as analyzed using multiplex proximity extension assays
Qiujin Shen, Johan Björkesten, Joakim Galli, Daniel Ekman, John Broberg, Niklas Nordberg, Annika Tillander, Masood Kamali-Moghaddam, Gunnel Tybring, Ulf Landegren
Clinical Chemistry and Laboratory Medicine (CCLM).2018; 56(4): 582. CrossRef
Plasma-Derived Inflammatory Proteins Predict Oral Squamous Cell Carcinoma
Kelly Yi Ping Liu, Xian Jun David Lu, Yuqi Sarah Zhu, Nhu Le, Hugh Kim, Catherine F. Poh
Frontiers in Oncology.2018;[Epub] CrossRef
Is vascular endothelial growth factor a useful biomarker in giant cell arteritis?
Nicola Goodfellow, Julien Morlet, Surjeet Singh, Afsie Sabokbar, Andrew Hutchings, Vanshika Sharma, Jana Vaskova, Shauna Masters, Allahdad Zarei, Raashid Luqmani
RMD Open.2017; 3(1): e000353. CrossRef
Indices of immune function used by ecologists are mostly unaffected by repeated freeze-thaw cycles and methodological deviations
Arne Hegemann, Sara Pardal, Kevin D. Matson
Frontiers in Zoology.2017;[Epub] CrossRef
The Enhanced Liver Fibrosis (ELF) Panel: Analyte Stability Under Common Sample Storage Conditions Used in Clinical Practice
Oliver J Kennedy, Julie Parkes, Sudeep Tanwar, Paul M Trembling, William M Rosenberg
The Journal of Applied Laboratory Medicine.2017; 1(6): 720. CrossRef
Instability of Plasma and Serum Progastrin-Releasing Peptide During Repeated Freezing and Thawing
Jae-Eun Lee, Jin-Hyun Lee, Maria Hong, Seul-Ki Park, Ji-In Yu, So-Youn Shin, Shine Young Kim
Osong Public Health and Research Perspectives.2016; 7(6): 351. CrossRef
Effects of Long-Term Storage Time and Original Sampling Month on Biobank Plasma Protein Concentrations
Stefan Enroth, Göran Hallmans, Kjell Grankvist, Ulf Gyllensten
EBioMedicine.2016; 12: 309. CrossRef

Aging-related Changes in Mouse Serum Glycerophospholipid Profiles: Seungwoo Kim, Hyo-Soon Cheon, Jae-Chun Song, Sang-Moon Yun, Sang Ick Park, Jae-Pil Jeon; Osong Public Health Res Perspect. 2014;5(6):345-350. Published online December 31, 2014; DOI: https://doi.org/10.1016/j.phrp.2014.10.002

3,973 View
27 Download
22 Crossref

Abstract PDF

Objectives
Metabolic dysfunction is a common hallmark of the aging process and aging-related pathogenesis. Blood metabolites have been used as biomarkers for many diseases, including cancers, complex chronic diseases, and neurodegenerative diseases.
Methods
In order to identify aging-related biomarkers from blood metabolites, we investigated the specific metabolite profiles of mouse sera from 4-month-old and 21-month-old mice by using a combined flow injection analysis–tandem mass spectrometry and liquid chromatography–tandem mass spectrometry.
Results
Among the 156 metabolites detected, serum levels of nine individual metabolites were found to vary with aging. Specifically, lysophosphatidylcholine (LPC) acyl (a) C24:0 levels in aged mice were decreased compared to that in young mice, whereas phosphatidylcholine (PC) acyl-alkyl (ae) C38:4, PC ae C40:4, and PC ae C42:1 levels were increased. Three classes of metabolites (amino acids, LPCs, and PCs) differed in intraclass correlation patterns of the individual metabolites between sera from young and aged mice. Additionally, the ratio of LPC a C24:0 to PC ae C38:4 was decreased in the aged mice, whereas the ratio of PC ae C40:4 to LPC a C24:0 was increased, supporting the aging-related metabolic changes of glycerophospholipids.
Conclusion
The ratios of the individual metabolites PC and LPC could serve as potential biomarkers for aging and aging-related diseases.

Citations

Citations to this article as recorded by

Age-related changes in antigen-specific natural antibodies are influenced by sex
Sarah E. Webster, Naomi L. Tsuji, Michael J. Clemente, Nichol E. Holodick
Frontiers in Immunology.2023;[Epub] CrossRef
Age-related changes in adipose tissue metabolomics and inflammation, cardiolipin metabolism, and ferroptosis markers in female aged rat model
Zhuang-Zhi Wang, Fang-Hui Li, Pin-Shi Ni, Lei Sun, Chen-Kai Zhang, Bo-Ming Li, Jia-Han He, Xiao-Ming Yu, Yun-Qing Liu
Biochemical and Biophysical Research Communication.2023; 671: 292. CrossRef
Apolipoprotein E knockout may affect cognitive function in D-galactose-induced aging mice through the gut microbiota–brain axis
Bowei Chen, Jian Yi, Yaqian Xu, Huiqiao Wen, Fengming Tian, Yingfei Liu, Lan Xiao, Lisong Li, Baiyan Liu
Frontiers in Neuroscience.2022;[Epub] CrossRef
Posterior subcapsular cataracts are a late effect after acute exposure to 0.5 Gy ionizing radiation in mice
Sarah Kunze, Alexander Cecil, Cornelia Prehn, Gabriele Möller, Andreas Ohlmann, Gerhild Wildner, Stephan Thurau, Kristian Unger, Ute Rößler, Sabine M. Hölter, Soile Tapio, Florian Wagner, Andreas Beyerlein, Fabian Theis, Horst Zitzelsberger, Ulrike Kulka,
International Journal of Radiation Biology.2021; 97(4): 529. CrossRef
Noninvasive Analysis Using Data‐Independent Acquisition Mass Spectrometry: New Epidermal Proteins That Reveal Sex Differences in the Aging Process
Shirui Chen, Hui Zhang, Mengting Liu, Yaochi Wang, Cong Xin, Jing Ma, Xiaodong Zheng, Xuejun Zhang, Liangdan Sun, Sen Yang, Alexandros Georgakilas
Oxidative Medicine and Cellular Longevity.2021;[Epub] CrossRef
Phospholipases and Reactive Oxygen Species Derived Lipid Biomarkers in Healthy and Diseased Humans and Animals – A Focus on Lysophosphatidylcholine
Kathrin M. Engel, Jürgen Schiller, Christina E. Galuska, Beate Fuchs
Frontiers in Physiology.2021;[Epub] CrossRef
Metabolomics Study of Isocaloric Different Dietary Patterns on the Life Span in Healthy Population
Cong Zhang, Qingna Yan, Qiushuang Zhu, Jinxiao Liu, Yuanjie Dong, Yuqiao Li, Ruohua Wang, Xuanfeng Tang, Xinyi Lv, Xiaoqing Li, Yunjiang Cai, Yucun Niu
Clinical Interventions in Aging.2021; Volume 16: 2111. CrossRef
Increased plasma phosphatidylcholine/lysophosphatidylcholine ratios in patients with Parkinson's disease
Jadranka Miletić Vukajlović, Dunja Drakulić, Snežana Pejić, Tihomir V. Ilić, Aleksandra Stefanović, Marijana Petković, Jürgen Schiller
Rapid Communications in Mass Spectrometry.2020;[Epub] CrossRef
Effects of lifelong exercise and aging on the blood metabolic fingerprint of rats
Anastasia Tzimou, Dimitra Benaki, Stefanos Nikolaidis, Emmanuel Mikros, Ioannis Taitzoglou, Vassilis Mougios
Biogerontology.2020; 21(5): 577. CrossRef
Ginsenoside Rb1 retards aging process by regulating cell cycle, apoptotic pathway and metabolism of aging mice
Shujie Yu, Hui Xia, Yanlei Guo, Xiaoxian Qian, Xiaojuan Zou, Huabing Yang, Mingzhu Yin, Hongtao Liu
Journal of Ethnopharmacology.2020; 255: 112746. CrossRef
Exercise-induced recovery of plasma lipids perturbed by ageing with nanoflow UHPLC-ESI-MS/MS
Kang Uk Kim, Kyeong Jin Yoon, Suhong Park, Jong Cheol Lee, Hyo Youl Moon, Myeong Hee Moon
Analytical and Bioanalytical Chemistry.2020; 412(28): 8003. CrossRef
Mouse Age Matters: How Age Affects the Murine Plasma Metabolome
Patrick Pann, Martin Hrabě de Angelis, Cornelia Prehn, Jerzy Adamski
Metabolites.2020; 10(11): 472. CrossRef
Effects of Aging, Long-Term and Lifelong Exercise on the Urinary Metabolic Footprint of Rats
Anastasia Tzimou, Stefanos Nikolaidis, Olga Begou, Aikaterina Siopi, Olga Deda, Ioannis Taitzoglou, Georgios Theodoridis, Vassilis Mougios
Metabolites.2020; 10(12): 481. CrossRef
Trends of Food Supply and Nutrient Intake in South Korea Over the Past 30 Years
JONG-GYU KIM, JOONG-SOON KIM, JEONG - GYOO KIM
Current Research in Nutrition and Food Science Jou.2019; 7(1): 85. CrossRef
Whole Blood Transcriptome Analysis for Lifelong Monitoring in Elite Sniffer Dogs Produced by Somatic Cell Nuclear Transfer
Sun-A Ock, Inchul Choi, Gi-Sun Im, Jae Gyu Yoo
Cellular Reprogramming.2019; 21(6): 301. CrossRef
Retracted Article: High-throughput metabolomics identifies serum metabolic signatures in acute kidney injury using LC-MS combined with pattern recognition approach
Hai-Hong Li, Jian-Liang Pan, Su Hui, Xiao-Wei Ma, Zhi-Long Wang, Hui-Xin Yao, Jun-Feng Wang, Hong Li
RSC Advances.2018; 8(27): 14838. CrossRef
A Metabolomics Profiling of Glaucoma Points to Mitochondrial Dysfunction, Senescence, and Polyamines Deficiency
Stéphanie Leruez, Alexandre Marill, Thomas Bresson, Grégoire de Saint Martin, Adrien Buisset, Jeanne Muller, Lydie Tessier, Cédric Gadras, Christophe Verny, Philippe Gohier, Patrizia Amati-Bonneau, Guy Lenaers, Dominique Bonneau, Gilles Simard, Dan Milea,
Investigative Opthalmology & Visual Science.2018; 59(11): 4355. CrossRef
Lactobacillus acidophilus DDS-1 Modulates the Gut Microbiota and Improves Metabolic Profiles in Aging Mice
Ravichandra Vemuri, Tanvi Shinde, Rohit Gundamaraju, Shakuntla V. Gondalia, Avinash V. Karpe, David J. Beale, Christopher J. Martoni, Rajaraman Eri
Nutrients.2018; 10(9): 1255. CrossRef
Role of kinase-coupled TRP channels in mineral homeostasis
Vladimir Chubanov, Lorenz Mittermeier, Thomas Gudermann
Pharmacology & Therapeutics.2018; 184: 159. CrossRef
Sarcosine Is Uniquely Modulated by Aging and Dietary Restriction in Rodents and Humans
Ryan O. Walters, Esperanza Arias, Antonio Diaz, Emmanuel S. Burgos, Fangxia Guan, Simoni Tiano, Kai Mao, Cara L. Green, Yungping Qiu, Hardik Shah, Donghai Wang, Adam D. Hudgins, Tahmineh Tabrizian, Valeria Tosti, David Shechter, Luigi Fontana, Irwin J. Ku
Cell Reports.2018; 25(3): 663. CrossRef
Mild TBI Results in a Long-Term Decrease in Circulating Phospholipids in a Mouse Model of Injury
Tanja Emmerich, Laila Abdullah, Joseph Ojo, Benoit Mouzon, Thinh Nguyen, Gary S. Laco, Gogce Crynen, James E. Evans, Jon Reed, Michael Mullan, Fiona Crawford
NeuroMolecular Medicine.2017; 19(1): 122. CrossRef
Improved motor and cognitive performance with sodium nitrite supplementation is related to small metabolite signatures: a pilot trial in middle-aged and older adults
Jamie N. Justice, Lawrence C. Johnson, Allison E. DeVan, Charmion Cruickshank-Quinn, Nichole Reisdorph, Candace J. Bassett, Trent D. Evans, Forrest A. Brooks, Nathan S. Bryan, Michel B. Chonchol, Tony Giordano, Matthew B. McQueen, Douglas R. Seals
Aging.2015; 7(11): 1004. CrossRef

Article

Serum MicroRNA Expression Profiling in Mice Infected with Rabies Virus: Myung Guk Han, Jun-Sun Park, Cho Soon Lee, Young Eui Jeong, Jung Sang Ryou, Jung Eun Cho, Young Ran Ju, Kyoung-Ki Lee; Osong Public Health Res Perspect. 2011;2(3):186-191. Published online December 31, 2011; DOI: https://doi.org/10.1016/j.phrp.2011.11.043

4,815 View
28 Download
6 Crossref

Abstract PDF

Objectives
Serum or plasma microRNAs (miRNAs) are potential biomarkers for the diagnosis for cancer and prenatal diseases. This study was conducted to investigate whether rabies virus causes a change in serum miRNA expression.
Methods
ICR mice were intramuscularly inoculated with rabies virus and were sacrificed weekly to collect serum and brain tissue for 4 weeks postinoculation. Mice were assigned to four groups based on the results of indirect immunofluorescent assays, enzyme-linked immunosorbent assay, and nested reverse transcription-polymerase chain reaction and the expression profiles of serum miRNAs were compared using a commercial mouse miRNA expression profiling assay.
Results
The expression levels of miRNAs changed significantly with the different stages of the disease. The expression level of 94 serum miRNAs in infected mice changed at least twofold. Seven microRNAs of them were significantly upregulated or downregulated in all infected mice regardless of disease status. The number of miRNAs with an expression level change decreased with the progression of the disease. In a hierarchical cluster analysis, infected mice clustered into a group separate from uninfected control mice.
Conclusions
Based on the relationship of miRNAs to gene expression regulation, miRNAs may be candidates for the study of viral pathogenesis and could have potential as biomarkers.

Citations

Citations to this article as recorded by

Expression Profiling and Bioinformatics Analysis of CircRNA in Mice Brain Infected with Rabies Virus
Wen Zhao, Jingyin Su, Ningning Wang, Naiyu Zhao, Shuo Su
International Journal of Molecular Sciences.2021; 22(12): 6537. CrossRef
Altered microRNA expression in COVID-19 patients enables identification of SARS-CoV-2 infection
Ryan J. Farr, Christina L. Rootes, Louise C. Rowntree, Thi H. O. Nguyen, Luca Hensen, Lukasz Kedzierski, Allen C. Cheng, Katherine Kedzierska, Gough G. Au, Glenn A. Marsh, Seshadri S. Vasan, Chwan Hong Foo, Christopher Cowled, Cameron R. Stewart, Ron A. M
PLOS Pathogens.2021; 17(7): e1009759. CrossRef
Machine Learning Identifies Cellular and Exosomal MicroRNA Signatures of Lyssavirus Infection in Human Stem Cell-Derived Neurons
Ryan J. Farr, Nathan Godde, Christopher Cowled, Vinod Sundaramoorthy, Diane Green, Cameron Stewart, John Bingham, Carmel M. O’Brien, Megan Dearnley
Frontiers in Cellular and Infection Microbiology.2021;[Epub] CrossRef
MicroRNA Biomarkers for Infectious Diseases: From Basic Research to Biosensing
Leon Tribolet, Emily Kerr, Christopher Cowled, Andrew G. D. Bean, Cameron R. Stewart, Megan Dearnley, Ryan J. Farr
Frontiers in Microbiology.2020;[Epub] CrossRef
Circulating microRNA profiles of Hendra virus infection in horses
Christopher Cowled, Chwan-Hong Foo, Celine Deffrasnes, Christina L. Rootes, David T. Williams, Deborah Middleton, Lin-Fa Wang, Andrew G. D. Bean, Cameron R. Stewart
Scientific Reports.2017;[Epub] CrossRef
Systems Biomedicine of Rabies Delineates the Affected Signaling Pathways
Sadegh Azimzadeh Jamalkandi, Sayed-Hamidreza Mozhgani, Hamid Gholami Pourbadie, Mehdi Mirzaie, Farshid Noorbakhsh, Behrouz Vaziri, Alireza Gholami, Naser Ansari-Pour, Mohieddin Jafari
Frontiers in Microbiology.2016;[Epub] CrossRef

First
Prev
Page of 1
Next
Last

Search