Objectives
To examine the effect of neuraminidase (NA) mutations on the NA inhibitor (NAI) resistance phenotype, the recombinant influenza A/Chungbuk/4448/2008(H1N1) virus isolated in South Korea during the 2008–2009 season was generated by reverse genetics. Methods
Site-directed mutagenesis was introduced on the <i>NA</i> gene of A/Chungbuk/4448/2008(H1N1) virus, and a total of 23 single, double, and triple mutants were generated. Resistance phenotype of these recombinant viruses was determined by NA-inhibition (NAI) assays based on a fluorometric method using two NAIs (oseltamivir and zanamivir). Results
NA-inhibition assays showed that all the single and double mutants containing the Y275 except the single Y275-E119V mutant conferred important levels of resistance to oseltamivir, whereas all the single, double, and triple mutants containing the E119V mutation were associated with the resistance to zanamivir. Conclusion
Considering the effect of mutations in <i>NA</i> gene on the resistance to NAIs, it is important to monitor the possible emergence and dissemination of multidrug-resistant variants in the human population due to amino acid changes at <i>NA</i> gene as well as to develop novel NAIs.
Citations
Citations to this article as recorded by
Phylogenetic analysis and docking study of neuraminidase gene of influenza A/H1N1 viruses circulating in Iran from 2010 to 2019 Sina Moeini, Atefeh Mohebbi, Behrokh Farahmand, Parvaneh Mehrbod, Fatemeh Fotouhi Virus Research.2023; 334: 199182. CrossRef
SEQUENCE‐FREE PHYLOGENETICS WITH MASS SPECTROMETRY Kevin M. Downard Mass Spectrometry Reviews.2022; 41(1): 3. CrossRef
Next-Generation Sequencing: An Eye-Opener for the Surveillance of Antiviral Resistance in Influenza Laura A.E. Van Poelvoorde, Xavier Saelens, Isabelle Thomas, Nancy H. Roosens Trends in Biotechnology.2020; 38(4): 360. CrossRef
Mutation-induced spatial differences in neuraminidase structure and sensitivity to neuraminidase inhibitors Zhi-wei Yang, Dong-xiao Hao, Yi-zhuo Che, Jia-hui Yang, Lei Zhang, Sheng-li Zhang Chinese Physics B.2018; 27(1): 018704. CrossRef
Authorised medicinal product Aspecton® Oral Drops containing thyme extract KMTv24497 shows antiviral activity against viruses which cause respiratory infections Eva Lenz, Christin Müller, Ahmed Mostafa, Julia Dzieciolowski, Pumaree Kanrai, Sharmistha Dam, Ute Cwientzek, Lars-Norbert Prenner, Stephan Pleschka Journal of Herbal Medicine.2018; 13: 26. CrossRef
Ultrasensitive Fluorogenic Reagents for Neuraminidase Titration Zhizeng Gao, Masahiro Niikura, Stephen G. Withers Angewandte Chemie International Edition.2017; 56(22): 6112. CrossRef
Ultrasensitive Fluorogenic Reagents for Neuraminidase Titration Zhizeng Gao, Masahiro Niikura, Stephen G. Withers Angewandte Chemie.2017; 129(22): 6208. CrossRef
First
Prev
