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Original Article
- Development of a Diagnostic Kit to Detect
Cryptosporidium parvum andGiardia lamblia - Hyeng-Il Cheun, Byung-Suk Chung, Da-Won Ma, Bo-La Goo, Shin-Hyeong Cho, Mi-jung Ji, Won-Ja Lee
- Osong Public Health Res Perspect. 2013;4(3):146-151. Published online June 30, 2013
- DOI: https://doi.org/10.1016/j.phrp.2013.04.006
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- 24 Download
- 7 Crossref
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Abstract
PDF - Objectives
This study aims to develop a high-sensitivity antibody diagnostic kit that will enable a rapid and accurate detection of Cryptospofidium parvum and Giardia lamblia in patients with diarrhea.
Methods
The cultivated C. parvum oocysts and G. lamblia cysts in each calf and dog were injected to mice to obtain antibodies, which were titrated. Spleen cells of the immunized mouse were separated and blended with myelomas to produce hybrid cell lines that form monoclonal antibodies. Using ELISA method, antibodies that specifically respond to C. parvum and G.lamblia were then selected. The cells were injected into the abdominal cavity of a BALB/c mouse to isolate hydrops abdominis containing high level of antibodies. The IgG antibody was purified using protein G gel.
Results
The detection limit of monoclonal antibodies for Cryptosporidium parvum and Giardia lamblia was 125 oocysts/mL and 1250 cysts/mL, respectively. In addition, during testing they did not show cross-reactivity to viruses (n = 15), bacteria (n =17), and parasites (n = 9).
Conclusion
The rapid diagnostic antibody kit developed in this study, which specifically responds to C. parvum and G. lamblia, will be useful in detecting and monitoring diarrheal infections. -
Citations
Citations to this article as recorded by
- Comprehensive review of conventional and state-of-the-art detection methods of Cryptosporidium
George Luka, Ehsan Samiei, Nishat Tasnim, Arash Dalili, Homayoun Najjaran, Mina Hoorfar
Journal of Hazardous Materials.2022; 421: 126714. CrossRef - A review ofCryptosporidiumspp. and their detection in water
Eman M. Hassan, Banu Örmeci, Maria C. DeRosa, Brent R. Dixon, Syed A. Sattar, Asma Iqbal
Water Science and Technology.2021; 83(1): 1. CrossRef - Portable on-chip colorimetric biosensing platform integrated with a smartphone for label/PCR-free detection of Cryptosporidium RNA
George S. Luka, Ephraim Nowak, Quin Robert Toyata, Nishat Tasnim, Homayoun Najjaran, Mina Hoorfar
Scientific Reports.2021;[Epub] CrossRef - Nanomaterials‐based biosensors for detection of microorganisms and microbial toxins
Laura Sutarlie, Sian Yang Ow, Xiaodi Su
Biotechnology Journal.2017;[Epub] CrossRef - ACG Clinical Guideline: Diagnosis, Treatment, and Prevention of Acute Diarrheal Infections in Adults
Mark S Riddle, Herbert L DuPont, Bradley A Connor
American Journal of Gastroenterology.2016; 111(5): 602. CrossRef - Multiplex-Touchdown PCR to Simultaneously Detect Cryptosporidium parvum, Giardia lamblia, and Cyclospora cayetanensis, the Major Causes of Traveler’s Diarrhea
Ji-Hun Shin, Sang-Eun Lee, Tong Soo Kim, Da-Won Ma, Jong-Yil Chai, Eun-Hee Shin
The Korean Journal of Parasitology.2016; 54(5): 631. CrossRef - Advancing Cryptosporidium Diagnostics from Bench to Bedside
Raul V. Destura, Rohani B. Cena, Ma. Jowina H. Galarion, Coleen M. Pangilinan, Geraldine M. Arevalo, Ryan Oliver C. Alba, Joy Ann G. Petronio, Gielenny M. Salem, Brian Schwem, Jesus Emmanuel A. D. Sevilleja
Current Tropical Medicine Reports.2015; 2(3): 150. CrossRef
- Comprehensive review of conventional and state-of-the-art detection methods of Cryptosporidium
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