Osong Public Health and Research Perspectives

Original Articles

Characterization of Antimicrobial Susceptibility, Extended-Spectrum β-Lactamase Genes and Phylogenetic Groups of Enteropathogenic Escherichia coli Isolated from Patients with Diarrhea: Erfaneh Jafari, Saeid Mostaan, Saeid Bouzari; Osong Public Health Res Perspect. 2020;11(5):327-333. Published online October 22, 2020; DOI: https://doi.org/10.24171/j.phrp.2020.11.5.09

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Objectives

Infectious diarrhea is one of the most common causes of pediatric death worldwide and enteropathogenic Escherichia coli (EPEC) is one of the main causes. There are 2 subgroups of EPEC, typical and atypical, based on the presence or absence of bundle forming pili (bfp), of which atypical EPEC is considered less virulent, but not less pathogenic. Antimicrobial resistance towards atypical EPEC among children is growing and is considered a major problem. In this study the pattern of antibiotic resistance in clinical isolates was determined.

Methods

Using 130 isolates, antibiotic resistance patterns and phenotypes were assessed, and genotypic profiles of extended spectrum β-lactamase (ESBL) production using disc diffusion and PCR was carried out. Phylogenetic groups were analyzed using quadruplex PCR.

Results

There were 65 E. coli isolates identified as atypical EPEC by PCR, among which the highest antibiotic resistance was towards ampicillin, followed by trimethoprim-sulfamethoxazole, and tetracycline. Multidrug resistance was detected in 44.6% of atypical EPEC isolates. Around 33% of isolates were determined to be extended spectrum β-lactamase producers, and in 90% of isolates, genes responsible for ESBL production could be detected. Moreover, the majority of atypical EPEC strains belonged to Group E, followed by Groups B1, B2 and C.

Conclusion

High rates of multidrug resistance and ESBL production among atypical EPEC isolates warrant periodical surveillance studies to select effective antibiotic treatment for patients. It is considered a critical step to manage antibiotic resistance by avoiding unnecessary prescriptions for antibiotics.

Citations

Citations to this article as recorded by

Antibacterial Efficacy of Feline-Derived Lactic Acid Bacteria against Enteropathogenic Escherichia coli: A Comprehensive In Vitro Analysis
Weiwei Wang, Hao Dong, Qianqian Chen, Xiaohan Chang, Longjiao Wang, Chengyi Miao, Shuxing Chen, Lishui Chen, Ran Wang, Shaoyang Ge, Wei Xiong
Fermentation.2024; 10(10): 514. CrossRef
Phenotypic and molecular characterization of enteropathogenic Escherichia coli and Salmonella spp. causing childhood diarrhoea in Awka, South-Eastern Nigeria
Ifeanyi Emmanuel Nwike, Malachy Chigozie Ugwu, Peter Chika Ejikeugwu, Nonye Treasure Ujam, Ifeanyichukwu Romanus Iroha, Charles Okechukwu Esimone
Bulletin of the National Research Centre.2023;[Epub] CrossRef
Antimicrobial Resistance and Biofilm Formation of Escherichia coli Isolated from Pig Farms and Surroundings in Bulgaria
Mila D. Kaleva, Yana Ilieva, Maya Margaritova Zaharieva, Lyudmila Dimitrova, Tanya Chan Kim, Iva Tsvetkova, Yordan Georgiev, Petya Orozova, Krasimir Nedev, Hristo Najdenski
Microorganisms.2023; 11(8): 1909. CrossRef
Isolation and Molecular Characterization of Proteus mirabilis Bacteria from Poultry Meat in the Iraqi Market and a Study of their Resistance to some Antibiotics
Ammar H. Hamed, Ahmed I. Alnazzal
IOP Conference Series: Earth and Environmental Sci.2023; 1262(6): 062019. CrossRef
Virulence and phylogenetic analysis of enteric pathogenic Escherichia coli isolated from children with diarrhoea in South Africa
Ntando W. Alfinete, John Y. Bolukaoto, Lee Heine, Natasha Potgieter, Tobias G. Barnard
International Journal of Infectious Diseases.2022; 114: 226. CrossRef
Prevalence of extended-spectrum β-lactamases, AmpC, and carbapenemases in Proteus mirabilis clinical isolates
Mona Shaaban, Soha Lotfy Elshaer, Ola A. Abd El-Rahman
BMC Microbiology.2022;[Epub] CrossRef
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Anca Delia Mare, Cristina Nicoleta Ciurea, Adrian Man, Bianca Tudor, Valeriu Moldovan, Luminița Decean, Felicia Toma
Gastroenterology Insights.2021; 12(1): 28. CrossRef
Prevalence of Antibiotic-Resistant Escherichia coli Isolated from Swine Faeces and Lagoons in Bulgaria
Lyudmila Dimitrova, Mila Kaleva, Maya M. Zaharieva, Christina Stoykova, Iva Tsvetkova, Maya Angelovska, Yana Ilieva, Vesselin Kussovski, Sevda Naydenska, Hristo Najdenski
Antibiotics.2021; 10(8): 940. CrossRef

Epidemiological Characteristics of Field Tick-Borne Pathogens in Gwang-ju Metropolitan Area, South Korea, from 2014 to 2018: Jung Wook Park, Seung Hun Lee, Gi Seong Lee, Jin Jong Seo, Jae Keun Chung; Osong Public Health Res Perspect. 2020;11(4):177-184. Published online August 31, 2020; DOI: https://doi.org/10.24171/j.phrp.2020.11.4.06

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122 Download
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Abstract PDF

Objectives

The importance of tick-borne diseases is increasing because of climate change, with a lack of long-term studies on tick-borne pathogens in South Korea. To understand the epidemiological characteristics of tick-borne diseases, the monthly distribution of field ticks throughout the year was studied in South Korea between May 2014 and April 2018 in a cross sectional study.

Methods

The presence of various tick-borne pathogens (Rickettsia species, Borrelia species, Anaplasma phagocytophilum) was confirmed by using polymerase chain reaction, to provide information for a prevention strategy against tick-borne pathogenic infections, through increased understanding of the relationship between seasonal variation and risk of infection with Rickettsia species. This was performed using logistic regression analysis (SPSS 20, IBM, USA) of the data obtained from the study.

Results

During the study period there were 11,717 ticks collected and 4 species identified. Haemapysalis longicornis was the most common species (n = 10,904, 93.1%), followed by Haemapysalis flava (n = 656, 5.6%), Ixodes nipponensis (n = 151, 1.3%), and Amblyomma testudinarium (n = 6, 0.05%) The results of this cross-sectional study showed that Haemapysalis flava carried a higher risk of transmission of Rickettsia species than other tick species (p < 0.05).

Conclusion

In conclusion, due attention should be paid to preventing tick-borne infections in humans whilst engaged in outdoor activities in Spring and Autumn, particularly in places where there is a high prevalence of ticks.

Citations

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Scientific Reports.2024;[Epub] CrossRef
Molecular Identification of Spotted Fever Group Rickettsiae in Ticks in the Republic of Korea
Ji-Ye Seo, Jin-Seo Park, Hee-Il Lee, Jung-Won Ju
Pathogens.2024; 13(7): 575. CrossRef
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Taeuk Kang, Yeon-Joo Choi, Jeoungyeon Kim, Hye-Jin Park, Won-Jong Jang
European Journal of Clinical Microbiology & Infect.2024; 43(10): 1909. CrossRef
Effective Methods of Estimation of Pathogen Prevalence in Pooled Ticks
Gerardo Fracasso, Marika Grillini, Laura Grassi, Francesco Gradoni, Graziana da Rold, Michela Bertola
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Graham A. Matulis, Jira Sakolvaree, Bazartseren Boldbaatar, Nora Cleary, Ratree Takhampunya, B. Katherine Poole-Smith, Abigail A. Lilak, Doniddemberel Altantogtokh, Nyamdorj Tsogbadrakh, Nitima Chanarat, Nittayaphon Youngdech, Erica J. Lindroth, Jodi M. F
Ticks and Tick-borne Diseases.2023; 14(5): 102203. CrossRef
Molecular Detection of Anaplasma phagocytophilum and Ehrlichia Species in Ticks Removed from Humans in the Republic of Korea
Yu-Jung Kim, Ji Ye Seo, Seong Yoon Kim, Hee Il Lee
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Molecular Detection and Phylogeny of Tick-Borne Pathogens in Ticks Collected from Dogs in the Republic of Korea
A-Tai Truong, Jinhyeong Noh, Yeojin Park, Hyun-Ji Seo, Keun-Ho Kim, Subin Min, Jiyeon Lim, Mi-Sun Yoo, Heung-Chul Kim, Terry A. Klein, Hyunkyoung Lee, Soon-Seek Yoon, Yun Sang Cho
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iSeq 100 for metagenomic pathogen screening in ticks
Ju Yeong Kim, Myung-hee Yi, Alghurabi Areej Sabri Mahdi, Tai-Soon Yong
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Insects.2021; 12(11): 1011. CrossRef

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