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Original Articles
Complete Sequence Analysis and Antiviral Screening of Medicinal Plants for Human Coxsackievirus A16 Isolated in Korea
Jae-Hyoung Song, Kwisung Park, Aeri Shim, Bo-Eun Kwon, Jae-Hee Ahn, Young Jin Choi, Jae Kyung Kim, Sang-Gu Yeo, Kyungah Yoon, Hyun-Jeong Ko
Osong Public Health Res Perspect. 2015;6(1):52-58.   Published online February 28, 2015
DOI: https://doi.org/10.1016/j.phrp.2014.12.004
  • 2,719 View
  • 17 Download
  • 15 Crossref
AbstractAbstract PDF
Objectives
Coxsackievirus A group 16 strain (CVA16) is one of the predominant causative agents of hand, foot, and mouth disease (HFMD).
Methods
Using a specimen from a male patient with HFMD, we isolated and performed sequencing of the Korean CVA16 strain and compared it with a G10 reference strain. Also, we were investigated the effects of medicinal plant extract on the cytopathic effects (CPE) by CPE reduction assay against Korean CVA16.
Results
Phylogenetic analysis showed that the Korean CVA16 isolate belonged to cluster B-1 and was closely related to the strain PM-15765-00 isolated in Malaysia in 2000. The Korean CVA16 isolate showed 73.2% nucleotide identity to the G10 prototype strain and 98.7% nucleotide identity to PM-15765-00. Next, we assessed whether the Korean CVA16 isolate could be used for in vitro screening of antiviral agents to treat HFMD infection. Vero cells infected with the Korean CVA16 isolate showed a cytopathic effect 2 days after the infection, and the treatment of cells with Cornus officinalis, Acer triflorum, Pulsatilla koreana, and Clematis heracleifolia var. davidiana Hemsl extracts exhibited strong antiviral activity against CVA16.
Conclusion
Collectively, our work provides potential candidates for the development of vaccine and novel drugs to treat the CVA16 strain isolated from a Korean patient.

Citations

Citations to this article as recorded by  
  • Recent advances in anti-coxsackievirus A16 viral drug research
    Xiaolan Yuan, Ziwei Liu, Li Wan, Wei Liu, Yan Huang, Shuang Cao
    Future Medicinal Chemistry.2023; 15(1): 97.     CrossRef
  • A Review with Updated Perspectives on the Antiviral Potentials of Traditional Medicinal Plants and Their Prospects in Antiviral Therapy
    Nur Fadlin Saifulazmi, Emelda Rosseleena Rohani, Sarahani Harun, Hamidun Bunawan, Hamizah Shahirah Hamezah, Nor Azlan Nor Muhammad, Kamalrul Azlan Azizan, Qamar Uddin Ahmed, Sharida Fakurazi, Ahmed Mediani, Murni Nazira Sarian
    Life.2022; 12(8): 1287.     CrossRef
  • Medicinal plants: Treasure for antiviral drug discovery
    Sofi Imtiyaz Ali, Wajid Mohammad Sheikh, Muzafar Ahmad Rather, Venugopalan Venkatesalu, Showkeen Muzamil Bashir, Showkat Ul Nabi
    Phytotherapy Research.2021; 35(7): 3447.     CrossRef
  • Seasonal Distribution and Meteorological Factors Associated with Hand, Foot, and Mouth Disease among Children in Xi’an, Northwestern China
    Tianci Guo, Jifeng Liu, Junjiang Chen, Yao Bai, Yong Long, Baozhong Chen, Shuxuan Song, Zhongjun Shao, Kun Liu
    The American Journal of Tropical Medicine and Hygi.2020; 102(6): 1253.     CrossRef
  • Short-term effects of meteorological factors and air pollution on childhood hand-foot-mouth disease in Guilin, China
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    Biosafety and Health.2019; 1(1): 32.     CrossRef
  • A potential therapeutic neutralization monoclonal antibody specifically against multi-coxsackievirus A16 strains challenge
    Ruixiao Du, Qunying Mao, Yalin Hu, Shuhui Lang, Shiyang Sun, Kelei Li, Fan Gao, Lianlian Bian, Ce Yang, Bopei Cui, Longfa Xu, Tong Cheng, Zhenglun Liang
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    Chinese Medicine.2018;[Epub]     CrossRef
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    Dong Joo Seo, Changsun Choi
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    Dong Joo Seo, Minhwa Lee, Su Been Jeon, Hyunkyung Park, Suntak Jeong, Bog-Hieu Lee, Changsun Choi
    Food Control.2017; 72: 9.     CrossRef
  • A novel Enterovirus 96 circulating in China causes hand, foot, and mouth disease
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  • Epidemiological Research on Hand, Foot, and Mouth Disease in Mainland China
    Zhi-Chao Zhuang, Zeng-Qiang Kou, Yong-Juan Bai, Xiang Cong, Li-Hong Wang, Chun Li, Li Zhao, Xue-Jie Yu, Zhi-Yu Wang, Hong-Ling Wen
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Ovicidal and Oviposition Deterrent Activities of Medicinal Plant Extracts Against Aedes aegypti L. and Culex quinquefasciatus Say Mosquitoes (Diptera: Culicidae)
Appadurai Daniel Reegan, Munusamy Rajiv Gandhi, Micheal Gabriel Paulraj, Savarimuthu Ignacimuthu
Osong Public Health Res Perspect. 2015;6(1):64-69.   Published online February 28, 2015
DOI: https://doi.org/10.1016/j.phrp.2014.08.009
  • 3,461 View
  • 29 Download
  • 42 Crossref
AbstractAbstract PDF
Objectives
To evaluate the ovicidal and oviposition deterrent activities of five medicinal plant extracts namely Aegle marmelos (Linn.), Limonia acidissima (Linn.), Sphaeranthus indicus (Linn.), Sphaeranthus amaranthoides (burm.f), and Chromolaena odorata (Linn.) against Culex quinquefasciatus and Aedes aegypti mosquitoes. Three solvents, namely hexane, ethyl acetate, and methanol, were used for the preparation of extracts from each plant.
Methods
Four different concentrations—62.5 parts per million (ppm), 125 ppm, 250 ppm, and 500 ppm—were prepared using acetone and tested for ovicidal and oviposition deterrent activities. One-way analysis of variance (ANOVA) was used to determine the significance of the treatments and means were separated by Tukey's test of comparison.
Results
Among the different extracts of the five plants screened, the hexane extract of L. acidissima recorded the highest ovicidal activity of 79.2% and 60% at 500 ppm concentration against the eggs of Cx. quinquefasciatus and Ae. aegypti, respectively. Similarly, the same hexane extract of L. acidissima showed 100% oviposition deterrent activity at all the tested concentrations against Cx. quinquefasciatus and Ae. aegypti adult females.
Conclusion
It is concluded that the hexane extract of L. acidissima could be used in an integrated mosquito management program.

Citations

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PHRP : Osong Public Health and Research Perspectives