Osong Public Health and Research Perspectives

Original Articles

Immunoinformatics approach for design novel multi-epitope prophylactic and therapeutic vaccine based on capsid proteins L1 and L2 and oncoproteins E6 and E7 of human papillomavirus 16 and human papillomavirus 18 against cervical cancer: Nicholas Ryan, Sari Eka Pratiwi, Mardhia Mardhia, Ysrafil Ysrafil, Delima Fajar Liana, Mahyarudin Mahyarudin; Osong Public Health Res Perspect. 2024;15(4):307-328. Published online July 23, 2024; DOI: https://doi.org/10.24171/j.phrp.2024.0013

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Graphical Abstract Abstract PDF Supplementary Material

Objectives
This study aimed to identify the optimal protein construction for designing a multi-epitope vaccine with both prophylactic and therapeutic effects against cervical cancer, utilizing an immunoinformatics approach. The construction process involved using capsid epitopes L1 and L2, as well as oncoproteins E5, E6, and E7 from human papillomavirus (HPV) types 16 and 18.
Methods
An experimental in silico analysis with an immunoinformatics approach was used to develop 2 multi-epitope vaccine constructs (A and B). Further analysis was then conducted to compare the constructs and select the one with the highest potential against cervical cancer.
Results
This study produced 2 antigenic, non-allergenic, and nontoxic multi-epitope vaccine constructs (A and B), which exhibited the ideal physicochemical properties for a vaccine. Further analysis revealed that construct B effectively induced both cellular and humoral immune responses.
Conclusion
The multi-epitope vaccine construct B for HPV 16 and 18, designed for both prophylactic and therapeutic purposes, met the development criteria for a cervical cancer vaccine. However, these findings need to be validated through in vitro and in vivo experiments.

Citations

Citations to this article as recorded by

Modulation of epithelial homeostasis by HPV using Notch and Wnt
June See Chong, John Doorbar
Tumour Virus Research.2024; 18: 200297. CrossRef

No Detection of Episomal or Integrated High-Risk Human Papillomavirus in Nonsmall Cell Lung Carcinomas among Korean Population: Jeong Eun Lee, Yong-Moon Lee, In-Ock Seong, Min-Woong Kang, Choong Sik Lee, Kyung-Hee Kim; Osong Public Health Res Perspect. 2016;7(6):356-359. Published online December 31, 2016; DOI: https://doi.org/10.1016/j.phrp.2016.10.004

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Abstract PDF

Objectives
High-risk human papillomavirus (hrHPV) is known to be a representative cancer-causing agent in the genital and head and neck regions. Many studies have detected hrHPV DNA in nonsmall cell lung carcinoma. However, hrHPV–etiologic correlation in nonsmall cell lung carcinoma remains unclear. This study is designed to determine the prevalence of episomal or integrated hrHPV DNA in nonsmall cell lung carcinoma among the Korean population.
Methods
Surgically resected nonsmall cell lung carcinoma tissues, including 134 cases of squamous cell carcinoma (SqCC) and 99 cases of adenocarcinoma (ADC), were examined. In situ hybridization (ISH) for detecting episomal or integrated hrHPV DNA was performed using the INFORM HPV III Family 16 Probe (B) in the Ventana-validated assay. Anyplex II HPV28 detection kit based on real-time polymerase chain reaction was used for HPV DNA detection and genotyping.
Results
All members of the study population were of Korean ethnicity. Episomal or integrated hrHPV DNA ISH analysis result was negative in all 233 cases. One SqCC of 89 samples (42 SqCCs and 47 ADCs) was positive for an hrHPV genotype by Anyplex II HPV28 detection kit.
Conclusion
Our finding did not demonstrate hrHPV–etiologic correlation in primary lung SqCC and ADC in the Korean population.

Citations

Citations to this article as recorded by

HPV and Lung Cancer: A Systematic Review
Telma Sequeira, Rui Pinto, Carlos Cardoso, Catarina Almeida, Rita Aragão, Teresa Almodovar, Manuel Bicho, Maria Clara Bicho, Cristina Bárbara
Cancers.2024; 16(19): 3325. CrossRef
HPV and lung cancer: A systematic review and meta‐analysis
Julia Karnosky, Wolfgang Dietmaier, Helge Knuettel, Viola Freigang, Myriam Koch, Franziska Koll, Florian Zeman, Christian Schulz
Cancer Reports.2021;[Epub] CrossRef
Presence and activity of HPV in primary lung cancer
Talita Helena Araujo de Oliveira, Carolina Medeiros do Amaral, Bianca de França São Marcos, Kamylla Conceição Gomes Nascimento, Ana Carine de Miranda Rios, Dafne Carolina Alves Quixabeira, Maria Tereza Cartaxo Muniz, Jacinto da Costa Silva Neto, Antonio C
Journal of Cancer Research and Clinical Oncology.2018; 144(12): 2367. CrossRef

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