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Original Article
- Molecular Classification of Human Adenovirus Type 7 Isolated From Acute Respiratory Disease Outbreak (ARD) in Korea, 2005–2006
- Wan Ji Lee, Chun Kang, Yoon Seok Chung, Kisoon Kim
- Osong Public Health Res Perspect. 2010;1(1):10-16. Published online December 31, 2010
- DOI: https://doi.org/10.1016/j.phrp.2010.12.005
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- 3 Crossref
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Abstract
PDF - Objectives
To assess the genomic characteristics of human adenoviruses (HAdVs) that caused small-scale epidemics in Korea and compare sequence analysis and restriction fragment length polymorphism (RFLP).
Methods
Two hundred sixty-two throat swabs were collected from geographically distinct two cohabitation facilities during outbreaks in August 2005 and February–May 2006. 148 isolates were obtained using the adenocarcinomic human alveolar basal epithelial cells (A549 cells) from 262 specimens. The sequences of 448 bp partial hexon gene of isolates were analized and compared with serotype results using neutralizing test. The hexon (1.2 kb), fiber, and E4 ORF 6/7 34.7 kDa protein (2.1 kb) genes were further analysed in 10 randomly selected specimens. RFLP of the genomic DNA for genotyping was also performed and compared with sequence information.
Results
All the isolates were localized into the same cluster when phylogenetic tree was generated based on hexon gene using Clustal W. While fiber and E4 ORF 6/7 34.7 kDa protein genes were analysed, the tree was divided into two clusters. Interestingly, isolates with same genetic characteristics of hexon gene did not show identical RFLP patterns in accordance with their origin of episode, rather phylogenetic analysis of fiber and E4 ORF 6/7 34.7 kDa protein genes were correlated with RFLP patterns.
Conclusion
These results indicate that serotype classification based on hexon gene might not be enough to discriminate HAdV serotype, and additional genetic characteristics including fiber and/or E4 ORF 6/7 should be recruited to dispose subgroup of HAdV serotype. -
Citations
Citations to this article as recorded by
- Diverse genotypes of human enteric and non-enteric adenoviruses circulating in children hospitalized with acute gastroenteritis in Thailand, from 2018 to 2021
Arpaporn Yodmeeklin, Kattareeya Kumthip, Nuthapong Ukarapol, Hiroshi Ushijima, Niwat Maneekarn, Pattara Khamrin, Day-Yu Chao
Microbiology Spectrum.2023;[Epub] CrossRef - Molecular epidemiology of a post-influenza pandemic outbreak of acute respiratory infections in Korea caused by human adenovirus type 3
Wan-Ji Lee, Hee-Dong Jung, Hyang-Min Cheong, Kisoon Kim
Journal of Medical Virology.2015; 87(1): 10. CrossRef - Antigenic variability among two subtypes of human adenovirus serotype 7
Xingui Tian, Xiaobo Su, Chunyan Xue, Xiao Li, Zhichao Zhou, Rong Zhou
Virus Genes.2014; 49(1): 22. CrossRef
- Diverse genotypes of human enteric and non-enteric adenoviruses circulating in children hospitalized with acute gastroenteritis in Thailand, from 2018 to 2021
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