Skip Navigation
Skip to contents

PHRP : Osong Public Health and Research Perspectives

OPEN ACCESS
SEARCH
Search

Search

Page Path
HOME > Search
1 "enterobacterial repetitive intergenic consensus-polymerase chain reaction"
Filter
Filter
Article category
Keywords
Publication year
Authors
Original Article
Plasmid-Mediated Quinolone-Resistance (qnr) Genes in Clinical Isolates of Escherichia coli Collected from Several Hospitals of Qazvin and Zanjan Provinces, Iran
Maryam Rezazadeh, Hamid Baghchesaraei, Amir Peymani
Osong Public Health Res Perspect. 2016;7(5):307-312.   Published online October 31, 2016
DOI: https://doi.org/10.1016/j.phrp.2016.08.003
  • 2,842 View
  • 26 Download
  • 28 Crossref
AbstractAbstract PDF
Objectives
Escherichia coli is regarded as the most important etiological agent of urinary tract infections. Fluoroquinolones are routinely used in the treatment of these infections; however, in recent years, a growing rate of resistance to these drugs has been reported globally. The aims of this study were to detect plasmid-mediated qnrA, qnrB, and qnrS genes among the quinolone-nonsusceptible E. coli isolates and to investigate their clonal relatedness in Qazvin and Zanjan Provinces, Iran.
Methods
A total of 200 clinical isolates of E. coli were collected from hospitalized patients. The bacterial isolates were identified through standard laboratory protocols and further confirmed using API 20E test strips. Antimicrobial susceptibility was determined by the standard disk diffusion method. Polymerase chain reaction (PCR) and sequencing were used for detecting qnrA, qnrB, and qnrS genes and the clonal relatedness of qnr-positive isolates was evaluated by enterobacterial repetitive intergenic consensus-PCR (ERIC-PCR) method.
Results
In total, 136 (68%) isolates were nonsusceptible to quinolone compounds, among which 45 (33.1%) and 71 (52.2%) isolates showed high- and low-level quinolone resistance, respectively. Of the 136 isolates, four (2.9%) isolates were positive for the qnrS1 gene. The results from ERIC-PCR revealed that two (50%) cases of qnr-positive isolates were related genetically.
Conclusion
Our study results were indicative of the presence of low frequency of qnr genes among the clinical isolates of E. coli in Qazvin and Zanjan Provinces, which emphasizes the need for establishing tactful policies associated with infection-control measures in our hospital settings.

Citations

Citations to this article as recorded by  
  • Polluted surface water is a repository of antibiotic-resistant Escherichia coli harbouring Plasmid-Mediated Quinolone Resistance (PMQR) determinants
    Abimbola Olumide Adekanmbi, Victoria Oluwanifemi Odunfa, Olabisi Comfort Akinlabi, Adedolapo Victoria Olaposi, Oreoluwa Samuel Adebowale, Zaccheaus Oluwaseun Faniran, Omowunmi Abosede Banjo
    International Journal of Environmental Studies.2024; : 1.     CrossRef
  • Phylogenetic analysis, biofilm formation, antimicrobial resistance and relationship between these characteristics in Uropathogenic Escherichia coli
    Talieh Mostaghimi, Abazar Pournajaf, Ali Bijani, Mohsen Mohammadi, Mehdi Rajabnia, Mehrdad Halaji
    Molecular Biology Reports.2024;[Epub]     CrossRef
  • Panel of primers for evaluation of antibiotic resistance genes with real time detection of results
    A. S. Mokhov, A. D. Klimova, D. V. Azarov, A. E. Goncharov
    Russian Journal for Personalized Medicine.2023; 3(1): 72.     CrossRef
  • Antimicrobial resistance in bacteria isolated from peridomestic Rattus species: A scoping literature review
    Theethawat Uea-Anuwong, Kaylee A. Byers, Lloyd Christian Wahl, Omid Nekouei, Yrjo Tapio Grohn, Ioannis Magouras
    One Health.2023; 16: 100522.     CrossRef
  • Prevalence and mechanisms of ciprofloxacin resistance in Escherichia coli isolated from hospitalized patients, healthy carriers, and wastewaters in Iran
    Zohreh Neyestani, Farzad Khademi, Roghayeh Teimourpour, Mojtaba Amani, Mohsen Arzanlou
    BMC Microbiology.2023;[Epub]     CrossRef
  • Quinolone resistance and biofilm formation capability of uropathogenic Escherichia coli isolates from an Iranian inpatients’ population
    Elham Rastegar, Yalda Malekzadegan, Reza Khashei, Nahal Hadi
    Molecular Biology Reports.2023; 50(10): 8073.     CrossRef
  • Antibiotic-resistant Escherichia coli Undergoes a Change in mcr-1 and qnr-S Expression after being Exposed to Gamma Irradiation
    Ahmed G. Merdash, Gamal M. El-Sherbiny, Ahmed O. El-Gendy, Ahmed F. Azmy, Hussein M. El-Kabbany, Maged S. Ahmad
    Journal of Pure and Applied Microbiology.2023; 17(4): 2620.     CrossRef
  • Effect of ciprofloxacin and in vitro gut conditions on biofilm of Escherichia coli isolated from clinical and environmental sources
    Vankadari Aditya, Akshatha Kotian, Sreya Saikrishnan, Anusha Rohit, Divyashree Mithoor, Indrani Karunasagar, Vijaya Kumar Deekshit
    Journal of Applied Microbiology.2022; 132(2): 964.     CrossRef
  • Detection of qnrA, qnrB, and qnrS genes in Klebsiella pneumoniae and Escherichia coli isolates from leukemia patients
    Mahdane Roshani, Alireza Goodarzi, Ali Hashemi, Farhad Afrasiabi, Hossein Goudarzi, Mohammadreza Arabestani
    Reviews in Medical Microbiology.2022; 33(1): 14.     CrossRef
  • Decentralized systems for the treatment of antimicrobial compounds released from hospital aquatic wastes
    Manisha Sharma, Ankush Yadav, Kashyap Kumar Dubey, Joshua Tipple, Diganta Bhusan Das
    Science of The Total Environment.2022; 840: 156569.     CrossRef
  • Presence and Transfer of Antimicrobial Resistance Determinants in Escherichia coli in Pigs, Pork, and Humans in Thailand and Lao PDR Border Provinces
    Chanika Pungpian, Nuananong Sinwat, Sunpetch Angkititrakul, Rangsiya Prathan, Rungtip Chuanchuen
    Microbial Drug Resistance.2021; 27(4): 571.     CrossRef
  • Symptoms, risk factors, diagnosis and treatment of urinary tract infections
    Rajanbir Kaur, Rajinder Kaur
    Postgraduate Medical Journal.2021; 97(1154): 803.     CrossRef
  • Quinolone resistance (qnrA) gene in isolates of Escherichia coli collected from the Al-Hillah River in Babylon Province, Iraq
    Hawraa Mohammed Al-Rafyai, Mourouge Saadi Alwash, Noor Salman Al-Khafaji
    Pharmacia.2021; 68(1): 1.     CrossRef
  • Hypervirulent and hypermucoviscous extended-spectrum β-lactamase-producing Klebsiella pneumoniae and Klebsiella variicola in Chile
    F. Morales-León, A. Opazo-Capurro, C. Caro, N. Lincopan, A. Cardenas-Arias, F. Esposito, V. Illesca, M. L. Rioseco, M. Domínguez-Yévenes, C. A. Lima, H. Bello-Toledo, Gerardo González-Rocha
    Virulence.2021; 12(1): 35.     CrossRef
  • Surgical Antibiotic Prophylaxis in an Era of Antibiotic Resistance: Common Resistant Bacteria and Wider Considerations for Practice
    Bradley D Menz, Esmita Charani, David L Gordon, Andrew JM Leather, S Ramani Moonesinghe, Cameron J Phillips
    Infection and Drug Resistance.2021; Volume 14: 5235.     CrossRef
  • High prevalence of plasmid-mediated quinolone resistance (PMQR) among E. coli from aquatic environments in Bangladesh
    Mohammed Badrul Amin, Sumita Rani Saha, Md Rayhanul Islam, S. M. Arefeen Haider, Muhammed Iqbal Hossain, A. S. M. Homaun Kabir Chowdhury, Emily K. Rousham, Mohammad Aminul Islam, Abdelazeem Mohamed Algammal
    PLOS ONE.2021; 16(12): e0261970.     CrossRef
  • Development of quinolone resistance and prevalence of different virulence genes among Shigella flexneri and Shigella dysenteriae in environmental water samples
    B. Roy, S.K. Tousif Ahamed, B. Bandyopadhyay, N. Giri
    Letters in Applied Microbiology.2020; 71(1): 86.     CrossRef
  • Synergistic Activity of Fluoroquinolones Combining with Artesunate Against Multidrug-Resistant Escherichia coli
    SiMin Wei, YueFei Yang, Wei Tian, MingJiang Liu, ShaoJie Yin, JinGui Li
    Microbial Drug Resistance.2020; 26(1): 81.     CrossRef
  • Plasmid-Mediated Quinolone Resistance in Pseudomonas aeruginosa Isolated from Burn Patients in Tehran, Iran
    Azam Molapour, Amir Peymani, Parvaneh Saffarain, Narges Habibollah-Pourzereshki, Pooya Rashvand
    Infectious Disorders - Drug Targets .2020; 20(1): 49.     CrossRef
  • Plasmidic Fluoroquinolone Resistance Genes in Fluoroquinolone-Resistant and/or Extended Spectrum Beta-Lactamase-ProducingEscherichia coliStrains Isolated from Pediatric and Adult Patients Diagnosed with Urinary Tract Infection
    Melisa Akgoz, Irem Akman, Asuman Begum Ates, Cem Celik, Betul Keskin, Busra Betul Ozmen Capin, Zeynep Ceren Karahan
    Microbial Drug Resistance.2020; 26(11): 1334.     CrossRef
  • Characterization of Plasmid-Mediated Quinolone Resistance and Serogroup Distributions of Uropathogenic Escherichia coli among Iranian Kidney Transplant Patients
    Amin Sadeghi, Mehrdad Halaji, Amirhossein Fayyazi, Seyed Asghar Havaei, Yun Peng Chao
    BioMed Research International.2020; 2020: 1.     CrossRef
  • Antimicrobial resistance in fecal Escherichia coli isolated from poultry chicks in northern Iran
    Zohreh Pourhossein, Leila Asadpour, Hadi Habibollahi, Seyedeh Tooba Shafighi
    Gene Reports.2020; 21: 100926.     CrossRef
  • Determination of antibiotic resistance genes and virulence factors in Escherichia coli isolated from Turkish patients with urinary tract infection
    Azer Özad Düzgün, Funda Okumuş, Ayşegül Saral, Ayşegül Çopur Çiçek, Sedanur Cinemre
    Revista da Sociedade Brasileira de Medicina Tropic.2019;[Epub]     CrossRef
  • The emergence of antimicrobial resistance in environmental strains of the Bacteroides fragilis group
    Sebastian Niestępski, Monika Harnisz, Ewa Korzeniewska, Ma. Guadalupe Aguilera-Arreola, Araceli Contreras-Rodríguez, Zofia Filipkowska, Adriana Osińska
    Environment International.2019; 124: 408.     CrossRef
  • Evaluation of Quinolone Resistance in Escherichia coli Isolates Recovered from Urine and Feces of Patients with Acute or Recurrent Urinary Tract Infection
    Hossein Norouzian, Nader Shahrokhi, Shahram Sabeti, Saeid Bouzari, Mohammad Pooya
    Journal of Medical Microbiology and Infectious Dis.2019; 7(4): 120.     CrossRef
  • Plasmid-mediated quinolones resistance in clinically important bacteria
    M. Omidvar Panah, M. Najafi, A. Peymani
    The Journal of Qazvin University of Medical Scien.2018; 22(2): 90.     CrossRef
  • Detection of Plasmid-Mediated qnr Genes Among the Clinical Quinolone-Resistant Escherichia coli Strains Isolated in Tehran, Iran
    Reza Ranjbar, Sajjad S. Tolon, Mehrdad Sami, Reza Golmohammadi
    The Open Microbiology Journal.2018; 12(1): 248.     CrossRef
  • Characterization of Escherichia coli strains isolated from raw vegetables
    Pavel Pleva, Magda Janalí­ková, Silvie Pavlí­čková, Martin Lecomte, Tanguy Godillon, Ivan Holko
    Potravinarstvo Slovak Journal of Food Sciences.2018; 12(1): 304.     CrossRef

PHRP : Osong Public Health and Research Perspectives