Osong Public Health and Research Perspectives

Original Articles

Seroprevalence of Brucellosis in Human Immunodeficiency Virus Infected Patients in Hamadan, Iran: Fariba Keramat, Mohammad Mehdi Majzobi, Jalal Poorolajal, Zohreh Zarei Ghane, Maryam Adabi; Osong Public Health Res Perspect. 2017;8(4):282-288. Published online August 31, 2017; DOI: https://doi.org/10.24171/j.phrp.2017.8.4.09

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Objectives

Brucellosis is a systemic disease with a wide spectrum of clinical manifestations. This study aimed to determine the seroprevalence of brucellosis in human immunodeficiency virus (HIV) infected patients in Hamadan Province in the west of Iran.

Methods

A total of 157 HIV-infected patients were screened through standard serological tests, including Wright’s test, Coombs’ Wright test, and 2-mercaptoethanol Brucella agglutination test (2ME test), blood cultures in Castaneda media, and CD4 counting. Data were analyzed using Stata version 11.

Results

Wright and Coombs’ Wright tests were carried out, and only 5 (3.2%) patients had positive serological results. However, all patients had negative 2ME results, and blood cultures were negative for Brucella spp. Moreover, patients with positive serology and a mean CD4 count of 355.8 ± 203.11 cells/μL had no clinical manifestations of brucellosis, and, and the other patients had a mean CD4 count of 335.55 ± 261.71 cells/μL.

Conclusion

Results of this study showed that HIV infection is not a predisposing factor of acquiring brucellosis.

Citations

Citations to this article as recorded by

A case of brucellosis concomitant with HIV infection in China
Shuai-Bing Dong, Li-Ping Wang, Chao-Xue Wu, Fan Li, Yong Yue, Dong-Ri Piao, Hong-Yan Zhao, Hai Jiang
Infectious Diseases of Poverty.2020;[Epub] CrossRef
Investigation of Linc-MAF-4 expression as an effective marker in brucellosis
Reza Gheitasi, Fariba Keramat, Ghasem Solgi, Mehrdad Hajilooi
Molecular Immunology.2020; 123: 60. CrossRef
Human Brucellosis: Risks and Prevalence among Iranian Blood Donors Residing in Endemic Areas
Maryam Zadsar, Mohammad Reza Shirzadi, Mohammad Zeynali, Mahboubeh Rasouli, Gharib Karimi
Transfusion Medicine and Hemotherapy.2020; 47(2): 103. CrossRef
Prevalence and risk factors of brucellosis among febrile patients attending a community hospital in south western Uganda
Richard Migisha, Dan Nyehangane, Yap Boum, Anne-Laure Page, Amaia Zúñiga-Ripa, Raquel Conde-Álvarez, Fred Bagenda, Maryline Bonnet
Scientific Reports.2018;[Epub] CrossRef

A Novel PCR Assay for Detecting Brucella abortus and Brucella melitensis: Saeed Alamian, Majid Esmaelizad, Taghi Zahraei, Afshar Etemadi, Mohsen Mohammadi, Davoud Afshar, Soheila Ghaderi; Osong Public Health Res Perspect. 2017;8(1):65-70. Published online February 28, 2017; DOI: https://doi.org/10.24171/j.phrp.2017.8.1.09

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Abstract PDF

Objectives

Brucellosis is a major zoonotic disease that poses a significant public health threat worldwide. The classical bacteriological detection process used to identify Brucella spp. is difficult and time-consuming. This study aimed to develop a novel molecular assay for detecting brucellosis.

Methods

All complete sequences of chromosome 1 with 2.1-Mbp lengths were compared among all available Brucella sequences. A unique repeat sequence (URS) locus on chromosome 1 could differentiate Brucella abortus from Brucella melitensis. A primer set was designed to flank the unique locus. A total of 136 lymph nodes and blood samples were evaluated and classified by the URS-polymerase chain reaction (PCR) method in 2013–2014.

Results

Biochemical tests and bacteriophage typing as the golden standard indicated that all Brucella spp. isolates were B. melitensis biovar 1 and B. abortus biovar 3. The PCR results were the same as the bacteriological method for detecting Brucella spp. The sensitivity and specificity of the URS-PCR method make it suitable for detecting B. abortus and B. melitensis.

Conclusion

Quick detection of B. abortus and B. melitensis can provide the most effective strategies for control of these bacteria. The advantage of this method over other presented methods is that both B. abortus and B. melitensis are detectable in a single test tube. Furthermore, this method covered 100% of all B. melitensis and B. abortus biotypes. The development of this URS-PCR method is the first step toward the development of a novel kit for the molecular identification of B. abortus and B. melitensis.

Citations

Citations to this article as recorded by

Investigating the Rate of Contamination of Milk and Traditional Dairy Products with Brucella Bacteria by PCR Method, Mamasani District, Fars Province, South of Iran
M Kalantari, N Askarpour, K Azizi, M Yousefi, M Amin, M Vahedi, A Keshavarz
Armaghane Danesh.2024; 29(2): 231. CrossRef
Development of a simplified and cost-effective sample preparation method for genotyping of human papillomavirus by next-generation sequencing
Rungrat Jitvaropas, Ukrit Thongpoom, Vorthon Sawaswong, Kritsada Khongnomnan, Witthaya Poomipak, Kesmanee Praianantathavorn, Pornjarim Nilyanimit, Yong Poovorawan, Sunchai Payungporn
Archives of Virology.2023;[Epub] CrossRef
Bovine brucellosis – a comprehensive review
Sandip Kumar Khurana, Anju Sehrawat, Ruchi Tiwari, Minakshi Prasad, Baldev Gulati, Muhammad Zubair Shabbir, Rajesh Chhabra, Kumaragurubaran Karthik, Shailesh Kumar Patel, Mamta Pathak, Mohd. Iqbal Yatoo, Vivek Kumar Gupta, Kuldeep Dhama, Ranjit Sah, Wanpe
Veterinary Quarterly.2021; 41(1): 61. CrossRef
Survey of Zoonotic Bacterial Pathogens in Native Foxes in Central Chile: First Record of Brucella canis Exposure
Nicolás Galarce, Sebastián de la Fuente, Beatriz Escobar, Phillip Dettleff, Pedro Abalos, Juan Carlos Hormazábal, Roberto Flores, Nicole Sallaberry-Pincheira, Víctor Martínez
Animals.2021; 11(7): 1980. CrossRef
Development and validation of immunoassay for whole cell detection of Brucella abortus and Brucella melitensis
Richa Hans, Pranjal Kumar Yadav, Pushpendra Kumar Sharma, Mannan Boopathi, Duraipandian Thavaselvam
Scientific Reports.2020;[Epub] CrossRef
Laboratory Diagnostic Procedures for Human Brucellosis: An Overview of Existing Approaches
Afshar Etemadi, Rezvan Moniri, Heinrich Neubauer, Yasaman Dasteh Goli, Saeed Alamian
Jundishapur Journal of Microbiology.2019;[Epub] CrossRef
Comparison of PCR-RFLP and PFGE for determining the clonality of Brucella isolates from human and livestock specimens
Nasrin Bahmani, Reza Mirnejad, Mohammad Reza Arabestani, Parviz Mohajerie, Seyed Hamid Hashemi, Manoochehr Karami, Mohammad Yousef Alikhani
Saudi Journal of Biological Sciences.2019; 26(2): 256. CrossRef
Designing an immunosensor for detection of Brucella abortus based on coloured silica nanoparticles
Arash Shams, Bahareh Rahimian Zarif, Mojtaba Salouti, Reza Shapouri, Sako Mirzaii
Artificial Cells, Nanomedicine, and Biotechnology.2019; 47(1): 2562. CrossRef
Identification of Brucella genus and eight Brucella species by Luminex bead-based suspension array
Tina S. Lusk Pfefer, Ruth Timme, Julie A. Kase
Food Microbiology.2018; 70: 113. CrossRef

Preparation and Evaluation of a New Lipopolysaccharide-based Conjugate as a Vaccine Candidate for Brucellosis: Seyed Davar Siadat, Farzam Vaziri, Mamak Eftekhary, Maryam Karbasian, Arfa Moshiri, Mohammad R. Aghasadeghi, Mehdi S. Ardestani, Meghdad Abdollahpour Alitappeh, Amin Arsang, Abolfazl Fateh, Shahin Najar Peerayeh, Ahmad R. Bahrmand; Osong Public Health Res Perspect. 2015;6(1):9-13. Published online February 28, 2015; DOI: https://doi.org/10.1016/j.phrp.2014.10.012

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Abstract PDF

Objectives
Development of an efficacious vaccine against brucellosis has been a challenge for scientists for many years. At present, there is no licensed vaccine against human brucellosis. To overcome this problem, currently, antigenic determinants of Brucella cell wall such as Lipopolysaccharide (LPS) are considered as potential candidates to develop subunit vaccines.
Methods
In this study, Brucella abortus LPS was used for conjugation to Neisseria meningitidis serogroup B outer membrane vesicle (OMV) as carrier protein using carbodiimide and adipic acid–mediated coupling and linking, respectively. Groups of eight BALB/c mice were injected subcutaneously with 10 μg LPS alone, combined LPS + OMV and conjugated LPS–OMV on 0 days, 14 days, 28 days and 42 days. Anti-LPS IgG was measured in serum.
Results
The yield of LPS to OMV in LPS–OMV conjugate was 46.55%, on the basis of carbohydrate content. The ratio for LPS to OMV was 4.07. The LPS–OMV conjugate was the most immunogenic compound that stimulated following the first injection with increased IgG titer of ∼5-fold and ∼1.3-fold higher than that produced against LPS and LPS in noncovalent complex to OMV (LPS + OMV), respectively. The highest anti-LPS IgG titer was detected 2 weeks after the third injection (Day 42) of LPS–OMV conjugate. The conjugated compound elicited higher titers of IgG than LPS + OMV, that showed a 100–120-fold rise of anti-LPS IgG in mice.
Conclusion
These results indicate that our conjugated LPS–OMV can be used as a brucellosis vaccine, but further investigation is required.

Citations

Citations to this article as recorded by

Engineered bacterial membrane vesicles are promising carriers for vaccine design and tumor immunotherapy
Qiong Long, Peng Zheng, Xiao Zheng, Weiran Li, Liangqun Hua, Zhongqian Yang, Weiwei Huang, Yanbing Ma
Advanced Drug Delivery Reviews.2022; 186: 114321. CrossRef
Isolation of Extracellular Vesicles of Akkermansia muciniphila as a Potential Therapeutic Platform
Pegah Noori, Fattah Sotoodehnejadnematalali, Pooneh Rahimi, Seyed davar Siadat
Vaccine Research.2022; 9(2): 27. CrossRef
Novel Simple Conjugation Chemistries for Decoration of GMMA with Heterologous Antigens
Roberta Di Benedetto, Renzo Alfini, Martina Carducci, Maria Aruta, Luisa Lanzilao, Alessandra Acquaviva, Elena Palmieri, Carlo Giannelli, Francesca Necchi, Allan Saul, Francesca Micoli
International Journal of Molecular Sciences.2021; 22(19): 10180. CrossRef
Outer membrane vesicle vaccines
Francesca Micoli, Calman A. MacLennan
Seminars in Immunology.2020; 50: 101433. CrossRef
Designing an immunosensor for detection of Brucella abortus based on coloured silica nanoparticles
Arash Shams, Bahareh Rahimian Zarif, Mojtaba Salouti, Reza Shapouri, Sako Mirzaii
Artificial Cells, Nanomedicine, and Biotechnology.2019; 47(1): 2562. CrossRef
Bioengineering bacterial outer membrane vesicles as vaccine platform
Matthias J.H. Gerritzen, Dirk E. Martens, René H. Wijffels, Leo van der Pol, Michiel Stork
Biotechnology Advances.2017; 35(5): 565. CrossRef

Frequencies of CD4+ T Regulatory Cells and their CD25^high and FoxP3^high Subsets Augment in Peripheral Blood of Patients with Acute and Chronic Brucellosis: Abbas Bahador, Jamshid Hadjati, Niloofar Hassannejad, Hadi Ghazanfari, Mohammadreza Maracy, Sirous Jafari, Maryam Nourizadeh, Amirhooshang Nejadeh; Osong Public Health Res Perspect. 2014;5(3):161-168. Published online June 30, 2014; DOI: https://doi.org/10.1016/j.phrp.2014.04.008

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Abstract PDF

Objectives
Brucellosis remains one of the most common zoonotic diseases worldwide. In humans, brucellosis can be a serious, debilitating, and sometimes chronic disease. Different mechanisms can be postulated as to the basis for the induction of the chronic status of infectious diseases that T regulatory cells are one of the most important related mechanisms. The current study was designed to determine whether percentage of CD4+Treg cells and their CD25^high and FoxP3^high subpopulations in peripheral blood are changed in human brucellosis samples in comparison to a control group.
Methods
In total, 68 brucellosis patients (acute form: n = 43, chronic form: n = 25) and 36 healthy volunteers entered our study. After isolating of peripheral blood mononuclear cells, heparinized venous blood samples were obtained from both patients and healthy donors, CD4, CD25, and FoxP3 molecules were evaluated by two- and three-color flow cytometric methods.
Results
The results revealed a new finding in relation to Treg cells and human brucellosis. The numbers of CD4⁺Treg cells and their CD25^high and FoxP3^high subsets increase significantly in the peripheral blood of acute and chronic forms of brucellosis samples compared with healthy groups, with this increase being greater in the chronic group.
Conclusion
There seems to be a correlation between increase of CD4+Treg cells and their subsets and the disease progress from healthy state to acute and chronic brucellosis.

Citations

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High-dimensional profiling of regulatory T cells in psoriasis reveals an impaired skin-trafficking property
Brian Hyohyoung Lee, Yoon Ji Bang, Sung Ha Lim, Seong-Jun Kang, Sung Hee Kim, Seunghee Kim-Schulze, Chung-Gyu Park, Hyun Je Kim, Tae-Gyun Kim
eBioMedicine.2024; 100: 104985. CrossRef
Investigation of IL-35 and IL-39, New Members of the IL-12 Family, in Different Clinical Presentations of Brucellosis
Pınar Hız Ellergezen, Muhammed Ali Kizmaz, Abdurrahman Simsek, Nesrin Demir, Eren Cagan, S. Haldun Bal, E. Halis Akalin, H. Barbaros Oral, Ferah Budak
Immunological Investigations.2023; 52(3): 286. CrossRef
In obese hypertensives cholecalciferol inhibits circulating TH17 cells but not macrophage infiltration on adipose tissue
Catarina Santos, Andreia Monteiro, Ricardo Rodrigues, Catarina Ferreira, Joana Coutinho, Rui Filipe, Sância Ramos, Miguel Castelo Branco, Mafalda Fonseca
Clinical Immunology.2023; 247: 109244. CrossRef
The mechanism of chronic intracellular infection with Brucella spp.
Xiaoyi Guo, Hui Zeng, Mengjuan Li, Yu Xiao, Guojing Gu, Zhenhui Song, Xuehong Shuai, Jianhua Guo, Qingzhou Huang, Bo Zhou, Yuefeng Chu, Hanwei Jiao
Frontiers in Cellular and Infection Microbiology.2023;[Epub] CrossRef
The potential role of infusions of T regulatory cells in inducing and maintaining liver allograft tolerance
Jihad Aljabban, William Burlingham, Michael R. Lucey
Clinical Liver Disease.2023; 22(4): 146. CrossRef
Intragraft regulatory T cells in the modern era: what can high-dimensional methods tell us about pathways to allograft acceptance?
Ke Fan Bei, Sajad Moshkelgosha, Bo Jie Liu, Stephen Juvet
Frontiers in Immunology.2023;[Epub] CrossRef
Immunosuppressive Mechanisms in Brucellosis in Light of Chronic Bacterial Diseases
Joaquin Miguel Pellegrini, Jean-Pierre Gorvel, Sylvie Mémet
Microorganisms.2022; 10(7): 1260. CrossRef
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Fabio Scirocchi, Simone Scagnoli, Andrea Botticelli, Alessandra Di Filippo, Chiara Napoletano, Ilaria Grazia Zizzari, Lidia Strigari, Silverio Tomao, Enrico Cortesi, Aurelia Rughetti, Paolo Marchetti, Marianna Nuti
eBioMedicine.2022; 79: 104010. CrossRef
Cell-Mediated Therapies to Facilitate Operational Tolerance in Liver Transplantation
Samia D. Ellias, Ellen L. Larson, Timucin Taner, Scott L. Nyberg
International Journal of Molecular Sciences.2021; 22(8): 4016. CrossRef
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Fahimeh Heidari, Mahboobeh Razmkhah, Vahid Razban, Nasrollah Erfani
Cell Biology International.2021; 45(12): 2544. CrossRef
Impact of immune checkpoint molecules on FoxP3+ Treg cells and related cytokines in patients with acute and chronic brucellosis
Hua-Li Sun, Xiu-Fang Du, Yun-Xia Tang, Guo-Qiang Li, Si-Yuan Yang, Ling-Hang Wang, Xing-Wang Li, Cheng-Jie Ma, Rong-Meng Jiang
BMC Infectious Diseases.2021;[Epub] CrossRef
Meta-Analysis of the Changes of Peripheral Blood T Cell Subsets in Patients with Brucellosis
Rongjiong Zheng, Songsong Xie, Shaniya Niyazi, Xiaobo Lu, Lihua Sun, Yan Zhou, Yuexin Zhang, Kai Wang
Journal of Immunology Research.2018; 2018: 1. CrossRef
Assessment of TGF-β and IL10 levels in human brucellosis
Mousa Mohammadnia-Afrouzi, Soheil Ebrahimpour
Current Issues in Pharmacy and Medical Sciences.2018; 31(1): 22. CrossRef

Evaluation and Selection of Multilocus Variable-Number Tandem-Repeat Analysis Primers for Genotyping Brucella abortus Biovar 1 Isolated from Human Patients: Subok Lee, Kyu-Jam Hwang, Mi-Yeoun Park, Seon-Do Hwang, Hee-Youl Chai, Hyuk Chu, Sang-Hee Park; Osong Public Health Res Perspect. 2013;4(5):265-270. Published online October 31, 2013; DOI: https://doi.org/10.1016/j.phrp.2013.09.005

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Abstract PDF

Objectives
Brucellosis is the most common bacterial zoonosis in the world. Multilocus variable-number tandem-repeat (VNTR) analysis (MLVA) is a molecular method for genotyping bacterial species. Brucella abortus biovar I was isolated from most of the brucellosis-suspected patients in Korea. This study was conducted to investigate the ability of various MLVA primers that are used for molecular typing B. abortus isolates and for analyzing their epidemiological data.
Methods
A total of 80 human isolates of B. abortus biovar I isolated from human patients and the reference strain were used for MLVA. Genetic diversity was determined by calculating the Simpson's diversity index (DI) of each VNTR locus. The Brucella strains were subcultured 30 times to determine the stability of each locus. The DNA of the strains cultivated in each passage was extracted and subjected to MLVA for further investigation.
Results
The 15 VNTR loci were selected based on high DI values. The DIs of the 15 VNTR loci showed considerable discrimination power ranging from 59% for Bruce 43 to 87% for Bruce 22. Bruce 09, Bruce 11, Bruce 16, Bruce 42, and Bruce 43 were confirmed to remain stable in vitro among the 15 VNTR loci selected.
Conclusion
The results of this study suggest that the five loci subsets may be a useful epidemiological tool for investigating B. abortus biovar 1 outbreak.

Citations

Citations to this article as recorded by

Brucella abortus: Current Research and Future Trends
Tariq Jamil, Falk Melzer, John Njeru, Hosny El-Adawy, Heinrich Neubauer, Gamal Wareth
Current Clinical Microbiology Reports.2017; 4(1): 1. CrossRef

Article

Application of the Microagglutination Test for Serologic Diagnosis of Human Brucellosis: Sang-Hee Park, Yoo-Hoon Lee, Hyuk Chu, Seon-Do Hwang, Kyu-Jam Hwang, Hee-Yeol Choi, Mi-Yeoun Park; Osong Public Health Res Perspect. 2012;3(1):19-23. Published online December 31, 2011; DOI: https://doi.org/10.1016/j.phrp.2012.01.003

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Abstract PDF

Objectives
Brucellosis is one of the most common zoonoses in the world, and occurs mainly in farmers, slaughterhouse workers, and veterinarians via direct or indirect contact with infected animals or their products. The clinical symptoms of human brucellosis are nonspecific, such as fever, headache, chills, and sweating. Diagnosis and treatment of brucellosis requires laboratory tests. Although the serum tube agglutination test (SAT) is the standardized gold method, it is laborious, time consuming, and requires a number of reagents. A microagglutination test (MAT) variant of the SAT or enzyme-linked immunosorbent assay (ELISA) is recommended for serological diagnoses. For the simple and rapid diagnosis of brucellosis, the MAT was standardized using samples for the SAT to define positive and negative categories, and we then compared the sensitivity and specificity of the MAT and ELISA.
Methods
Thirty SAT-positive sera and 60 SAT-negative sera were used in this study. Antibody titers of ≥1:160 were considered positive readings in both the SAT and MAT. Brucella abortus antigens and Brucella-positive control antiserum were used in the SAT and MAT. ELISAs of IgM and IgG were performed according to the manufacturers’ instructions.
Results
The titers of the MAT differed according to antigen concentration. The optimal concentration of B abortus antigen was determined to compare the sensitivity and specificity between the MAT and SAT. The sensitivity and specificity of the MAT were 93.3% and 96.7%, respectively, for IgG with reference to ELISA, and 96.7% and 98.3%, respectively, for IgM.
Conclusions
The optimal concentration of antigen for the MAT was 1:10. The MAT is less time consuming and requires less antigen and serum than the SAT. The results of the MAT showed good agreement with those of ELISA. The results of this study suggest that the MAT could be useful for diagnosis of brucellosis.

Citations

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The Development of Diagnostic and Vaccine Strategies for Early Detection and Control of Human Brucellosis, Particularly in Endemic Areas
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Vaccines.2023; 11(3): 654. CrossRef
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Ahmed M. Sallam, Ibrahim Abou-souliman, Henry Reyer, Klaus Wimmers, Alaa Emara Rabee
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Bovine brucellosis – a comprehensive review
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Veterinary Quarterly.2021; 41(1): 61. CrossRef
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Giovanni Di Bonaventura, Silvia Angeletti, Andrea Ianni, Tommasangelo Petitti, Giovanni Gherardi
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Peifeng Liang, Yuan Zhao, Jianhua Zhao, Dongfeng Pan, Zhongqin Guo
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Manuela Carugati, Holly M Biggs, Michael J Maze, Robyn A Stoddard, Shama Cash-Goldwasser, Julian T Hertz, Jo E B Halliday, Wilbrod Saganda, Bingileki F Lwezaula, Rudovick R Kazwala, Sarah Cleaveland, Venance P Maro, Matthew P Rubach, John A Crump
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Evaluation and Selection of Multilocus Variable-Number Tandem-Repeat Analysis Primers for Genotyping Brucella abortus Biovar 1 Isolated from Human Patients
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Osong Public Health and Research Perspectives.2013; 4(5): 265. CrossRef

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