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- Association between Beta-lactam Antibiotic Resistance and Virulence Factors in AmpC Producing Clinical Strains of
P. aeruginosa - Sanaz Dehbashi, Hamed Tahmasebi, Mohammad Reza Arabestani
- Osong Public Health Res Perspect. 2018;9(6):325-333. Published online December 31, 2018
- DOI: https://doi.org/10.24171/j.phrp.2018.9.6.06
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Abstract
PDF Objectives The purpose of this study was to determine the presence of
IMP andOXA genes in clinical strains ofPseudomonas aeruginosa (P. aeruginosa ) that are carriers of theampC gene.Methods In this study, 105 clinical isolates of
P. aeruginosa were collected. Antibiotic resistance patterns were determined using the disk diffusion method. The strains carrying AmpC enzymes were characterized by a combination disk method. Multiplex-PCR was used to identify resistance and virulence genes, chi-square test was used to determine the relationship between variables.Results Among 105 isolates of
P. aeruginosa , the highest antibiotic resistance was to cefotaxime and aztreonam, and the least resistance was to colictin and ceftazidime. There were 49 isolates (46.66%) that showed an AmpC phenotype. In addition, the frequencies of the resistance genes were;OXA48 gene 85.2%,OXA199, 139 3.8%,OXA23 3.8%,OXA2 66.6%,OXA10 3.8%,OXA51 85.2% andOXA58 3.8%. TheIMP27 gene was detected in 9 isolates (8.57%) and theIMP3.34 was detected in 11 isolates (10.47%). Other genes detected included;lasR (17.1%),lasB (18%) andlasA (26.6%). There was a significant relationship between virulence factors and theOX andIMP genes (p ≤ 0.05).Conclusion The relationship between antibiotic resistance and virulence factors observed in this study could play an important role in outbreaks associated with
P. aeruginosa infections.-
Citations
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