Osong Public Health and Research Perspectives

Original Article

Profiling Virulence and Antimicrobial Resistance Markers of Enterovirulent Escherichia Coli from Fecal Isolates of Adult Patients with Enteric Infections in West Cameroon: Wiliane J. T. Marbou, Priyanka Jain, Sriparna Samajpati, Gourab Halder, Asish K. Mukhopadhyay, Shanta Dutta, Victor Kuete; Osong Public Health Res Perspect. 2020;11(4):216-230. Published online August 31, 2020; DOI: https://doi.org/10.24171/j.phrp.2020.11.4.11

7,744 View
127 Download
4 Web of Science
5 Crossref

Objectives

This study aimed to identify virulent and antimicrobial resistant genes in fecal E. coli in Mbouda, Cameroon.

Methods

A total of 599 fecal samples were collected from patients with enteric infections who were ≥ 20 years old. E. coli was isolated on the MacConkey agar and virulent genes were detected by multiplex/simplex PCR. Isolates in which ≥ 1 virulent gene was detected were subjected to antibiotic susceptibility testing. The resulting resistant isolates were subjected to PCR, followed by sequencing for resistant genes detection.

Results

There were 119 enterovirulent E. coli identified, amongst which 47.05% were atypical enteropathogenic E. coli (EPEC), 36.97% enterotoxigenic E. coli, 10.08% Shiga toxin producing E. coli (STEC) and 5.88% were enteroinvasive E. coli (EIEC). The occurrence of the eae gene (47.06%) was higher compared with CVD432 (33.61%), aaic (13.45%), stx2 (10.08%) and stx1 (0.84%). High resistance rates were noted for ampicillin (94.64% EPEC, 91.67% STEC, 59.09% EAEC, and 57.14% EIEC) and sulfamethoxazole-trimethoprim (100% EPEC and 83.33% STEC, 81.82% EAEC and 71.43% EIEC). sul2 (71.43%), tetB (64.71%), tetA (59.94%) and blaTEM (52.10%) were detected. A double mutation (S83L; D87N) was seen in gyrA and a single mutation (S80I) was observed in parC.

Conclusion

These findings suggested that measures should be taken to reduce the harm of E. coli to public health.

Citations

Citations to this article as recorded by

Molecular epidemiology of antimicrobial resistance in central africa: A systematic review
Annicet-Clotaire Dikoumba, Richard Onanga, Laurette G. Mangouka, Larson Boundenga, Edgard-Brice Ngoungou, Sylvain Godreuil
Access Microbiology .2023;[Epub] CrossRef
Methanol extract from the seeds of Persea americana displays antibacterial and wound healing activities in rat model
Steve E. Ekom, Jean-De-Dieu Tamokou, Victor Kuete
Journal of Ethnopharmacology.2022; 282: 114573. CrossRef
Characterization of diarrhoeagenic Escherichia coli with special reference to antimicrobial resistance isolated from hospitalized diarrhoeal patients in Kolkata (2012–2019), India
Debjani Ghosh, Goutam Chowdhury, Prosenjit Samanta, Sreeja Shaw, Alok K. Deb, Mainak Bardhan, Asis Manna, Shin-ichi Miyoshi, Thandavarayan Ramamurthy, Shanta Dutta, Asish K. Mukhopadhyay
Journal of Applied Microbiology.2022; 132(6): 4544. CrossRef
Antibiotic resistomes and their chemical residues in aquatic environments in Africa
Aemere Ogunlaja, Olumuyiwa O. Ogunlaja, Olumide D. Olukanni, Gloria O. Taylor, Chidinma G. Olorunnisola, Victorien T. Dougnon, Wassiyath Mousse, Despo Fatta-Kassinos, Titus A.M. Msagati, Emmanuel I. Unuabonah
Environmental Pollution.2022; 312: 119783. CrossRef
Antibacterial and Therapeutic Potentials of the Capsicum annuum Extract against Infected Wound in a Rat Model with Its Mechanisms of Antibacterial Action
Steve Endeguele Ekom, Jean-De-Dieu Tamokou, Victor Kuete, Dorota Formanowicz
BioMed Research International.2021; 2021: 1. CrossRef

First
Prev
Page of 1
Next
Last

Search