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Prevalence of plasmid-mediated AmpC β-lactamases among uropathogenic Escherichia coli isolates in southwestern Iran
Nabi Jomehzadeh, Khadijeh Ahmadi, Zahra Rahmani
Osong Public Health Res Perspect. 2021;12(6):390-395.   Published online December 1, 2021
DOI: https://doi.org/10.24171/j.phrp.2021.0272
  • 6,091 View
  • 112 Download
  • 6 Web of Science
  • 6 Crossref
AbstractAbstract PDF
Objectives
This study was undertaken to evaluate AmpC β-lactamase-producing Escherichia coli urine isolates and to characterize the frequency of plasmid-mediated AmpC (pAmpC)-encoding genes.
Methods
Antimicrobial susceptibility tests were performed using the disk diffusion technique. AmpC β-lactamase production was assessed with a phenotypic inhibitor-based method. The presence of 6 pAmpC-encoding cluster genes was detected by multiplex polymerase chain reaction (PCR).
Results
The proportion of antibiotic resistance of E. coli isolates ranged from 7.4% to 90.5%, and more than half (51.6%) of the total isolates were multidrug-resistant (MDR). Among the 95 E. coli isolates, 60 (63.2%) were found to be cefoxitin-resistant, but only 14 (14.7%) isolates were confirmed as AmpC β-lactamase-producers. In the PCR assay, pAmpC-encoding genes were found in 15 (15.8%) isolates, and blaDHA was the most prevalent type. However, blaFOX, blaMOX, and blaACC genes were not detected in the isolates.
Conclusion
Our findings contributed valuable information concerning antibiotic resistance, confirmatory phenotypic testing for AmpC production, and pAmpC β-lactamase gene content in E. coli isolates in southwestern Iran. The level of MDR recorded in AmpC-producing strains of this study was worrying; therefore, implementing strong infection control approaches to reduce the MDR burden is recommended.

Citations

Citations to this article as recorded by  
  • A review of the mechanisms that confer antibiotic resistance in pathotypes of E. coli
    Sina Nasrollahian, Jay P. Graham, Mehrdad Halaji
    Frontiers in Cellular and Infection Microbiology.2024;[Epub]     CrossRef
  • Association Between Uropathogenic Escherichia coli Virulence Genes and Severity of Infection and Resistance to Antibiotics
    Sofía Alejandra Fonseca-Martínez, Ruth Aralí Martínez-Vega, Ana Elvira Farfán-García, Clara Isabel González Rugeles, Libeth Yajaira Criado-Guerrero
    Infection and Drug Resistance.2023; Volume 16: 3707.     CrossRef
  • Extended-spectrum β-lactamases producing Enterobacteriaceae (ESBL-PE) prevalence in Nepal: A systematic review and meta-analysis
    Christina Khadka, Manita Shyaula, Gopiram Syangtan, Shrijana Bista, Reshma Tuladhar, Anjana Singh, Dev Raj Joshi, Lok R. Pokhrel, Prabin Dawadi
    Science of The Total Environment.2023; 901: 166164.     CrossRef
  • Prevalence of Extended-Spectrum β-Lactamase-Resistant Genes in Escherichia coli Isolates from Central China during 2016–2019
    Zui Wang, Qin Lu, Xiaohui Mao, Li Li, Junfeng Dou, Qigai He, Huabin Shao, Qingping Luo
    Animals.2022; 12(22): 3191.     CrossRef
  • Molecular detection and characterization of Shigella spp. harboring extended-spectrum β-lactamase genes in children with diarrhea in northwest Iran
    Sahar Sabour, Amir Teimourpour, Jafar Mohammadshahi, Hadi Peeridogaheh, Roghayeh Teimourpour, Taher Azimi, Zahra Hosseinali
    Molecular and Cellular Pediatrics.2022;[Epub]     CrossRef
  • Plasmid-mediated AmpC β-Lactamase Gene Analysis in Klebsiella Pneumoniae Clinical Isolates
    Nabi Jomehzadeh, Khadijeh Ahmadi, Hasti Shaabaninejad, Gholamali Eslami
    Biomedical and Biotechnology Research Journal (BBR.2022; 6(4): 582.     CrossRef
Relationships between Virulence Factors and Antimicrobial Resistance among Escherichia coli Isolated from Urinary Tract Infections and Commensal Isolates in Tehran, Iran
Mohammad Reza Asadi Karam, Mehri Habibi, Saeid Bouzari
Osong Public Health Res Perspect. 2018;9(5):217-224.   Published online October 31, 2018
DOI: https://doi.org/10.24171/j.phrp.2018.9.5.02
  • 7,975 View
  • 157 Download
  • 33 Crossref
AbstractAbstract PDF
Objectives

Uropathogenic Escherichia coli (UPEC) are the major cause of urinary tract infections (UTIs). Here, we determined whether sensitivity to antibiotics was related to the prevalence of iron scavenging genes, or to biofilm and hemolysis formation.

Methods

A total of 110 UPEC and 30 E coli isolates were collected from the urine of UTI patients and feces of healthy individuals without UTI, respectively. The presence of iron receptor genes and phenotypic properties were evaluated by polymerase chain reaction and phenotypic methods, respectively. Susceptibility to routine antibiotics was evaluated using the disc diffusion method.

Results

The prevalence of iron scavenging genes ranged from 21.8% (ireA) to 84.5% (chuA) in the UPEC. Resistance to ceftazidime and cefotaxime was significantly correlated with the presence of fyuA and iutA iron genes. Biofilm production was significantly associated with the prevalence of fyuA and hma iron genes. A higher degree of antibiotic resistance was exhibited by isolates that produced biofilms than by their non-biofilm producing counterparts.

Conclusion

Our study clearly indicates that biofilm production is associated with antibiotic resistance, and that iron receptors and hemolysin production also contribute to reduced antibiotic sensitivity. These results further our understanding of the role that these virulence factors play during UPEC pathogenesis, which in turn may be valuable for the development of novel treatment strategies against UTIs.

Citations

Citations to this article as recorded by  
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    Sara A. Alshaikh, Tarek El-banna, Fatma Sonbol, Mahmoud H. Farghali
    Annals of Clinical Microbiology and Antimicrobials.2024;[Epub]     CrossRef
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    Frontiers in Veterinary Science.2024;[Epub]     CrossRef
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    Scientific Reports.2024;[Epub]     CrossRef
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    Yikun Xing, Justin R. Clark, James D. Chang, Jacob J. Zulk, Dylan M. Chirman, Felipe-Andres Piedra, Ellen E. Vaughan, Haroldo J. Hernandez Santos, Kathryn A. Patras, Anthony W. Maresso, Kimberly A. Kline
    Infection and Immunity.2024;[Epub]     CrossRef
  • Escherichia coli isolated from pyometra and cystitis in the same animal exhibit a wide phenotypic similarity
    Camila Azevedo Moni, Maria Eduarda Dias, Cassiane Elisabete Lopes, Franciele Maboni Siqueira
    Journal of Applied Microbiology.2024;[Epub]     CrossRef
  • Molecular Evaluation of Aminoglycoside Resistance and Biofilm Formation Potential in Escherichia coli Isolates Collected from Hospitalized Patients
    Sousan Akrami, Saeed Khoshnood, Maryam Koupaei, Effat Abbasi Montazeri, Hossein Meghdadi, Morteza Saki, Moloudsadat Motahar, Sara Masihzadeh, Sara Daneshfar, Marjan Abdi, Zahra Farshadzadeh
    Jundishapur Journal of Microbiology.2024;[Epub]     CrossRef
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    Shiela Chetri
    Microbial Pathogenesis.2024; : 107173.     CrossRef
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    Shane Whelan, Brigid Lucey, Karen Finn
    Microorganisms.2023; 11(9): 2169.     CrossRef
  • Susceptibility and Virulence of Enterobacteriaceae Isolated from Urinary Tract Infections in Benin
    Funkè F. Assouma, Haziz Sina, Tomabu Adjobimey, Agossou Damien Pacôme Noumavo, Akim Socohou, Bawa Boya, Ange D. Dossou, Lauriane Akpovo, Basile Boni Saka Konmy, Jacques F. Mavoungou, Adolphe Adjanohoun, Lamine Baba-Moussa
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    Sofía Alejandra Fonseca-Martínez, Ruth Aralí Martínez-Vega, Ana Elvira Farfán-García, Clara Isabel González Rugeles, Libeth Yajaira Criado-Guerrero
    Infection and Drug Resistance.2023; Volume 16: 3707.     CrossRef
  • Incidence of biofilms among the multidrug resistant E. coli, isolated from urinary tract infections in the Nilgiris district, South India
    A. P. Cardiliya, M. J. N. Chandrasekar, M. J. Nanjan
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    Hossein Karballaei Mirzahosseini, Farhad Najmeddin, Atabak Najafi, Arezoo Ahmadi, Hamidreza Sharifnia, Azad Khaledi, Mojtaba Mojtahedzadeh
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  • Insects, Rodents, and Pets as Reservoirs, Vectors, and Sentinels of Antimicrobial Resistance
    Willis Gwenzi, Nhamo Chaukura, Norah Muisa-Zikali, Charles Teta, Tendai Musvuugwa, Piotr Rzymski, Akebe Luther King Abia
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