Skip Navigation
Skip to contents

PHRP : Osong Public Health and Research Perspectives



Page Path
HOME > Search
1 "Radachlorin"
Article category
Publication year
Funded articles
Original Article
The effect of photodynamic therapy using Radachlorin on biofilm-forming multidrug-resistant bacteria
Choong-Won Seo, Young-Kwon Kim, Jeong-Lib An, Jong-Sook Kim, Pil-Seung Kwon, Young-Bin Yu
Osong Public Health Res Perspect. 2022;13(4):290-297.   Published online August 10, 2022
  • 2,420 View
  • 47 Download
  • 4 Web of Science
  • 4 Crossref
AbstractAbstract PDF
This study aimed to test the effect of photodynamic therapy (PDT) on the inhibition and removal of biofilms containing multidrug-resistant Acinetobacter baumannii.
Using multidrug-resistant A. baumannii strains, an antibiotic susceptibility test was performed using the Gram-negative identification card of the Vitek 2 system (bioMérieux Inc., France), as well as an analysis of resistance genes, the effects of treatment with a light-emitting diode (LED) array using Radachlorin (RADA-PHARMA Co., Ltd., Russia), and transmission and scanning electron microscopy to confirm the biofilm-inhibitory effect of PDT.
The antibiotic susceptibility test revealed multiple resistance to the antibiotics imipenem and meropenem in the carbapenem class. A class-D–type β-lactamase was found, and OXA-23 and OXA-51 were found in 100% of 15 A. baumannii strains. After PDT using Radachlorin, morphological observations revealed an abnormal structure due to the loss of the cell membrane and extensive morphological changes, including low intracellular visibility and small vacuoles attached to the cell membrane.
PDT involving a combination of LED and Radachlorin significantly eliminated the biofilm of multidrug-resistant A. baumannii. Observations made using electron microscopy showed that PDT combining LED and Radachlorin was effective. Additional studies on the effective elimination of biofilms containing multidrug-resistant bacteria are necessary, and we hope that a treatment method superior to sterilization with antibiotics will be developed in the future.


Citations to this article as recorded by  
  • Photophysical properties of Radachlorin photosensitizer in solutions of different pH, viscosity and polarity
    A.V. Belashov, A.A. Zhikhoreva, I.A. Gorbunova, M.E. Sasin, Sh.S. Shayakhmedov, I.V. Semenova
    Spectrochimica Acta Part A: Molecular and Biomolec.2024; 305: 123480.     CrossRef
  • Applications of Antimicrobial Photodynamic Therapy in Aquaculture: Effect on Fish Pathogenic Bacteria
    Edith Dube, Grace Emily Okuthe
    Fishes.2024; 9(3): 99.     CrossRef
  • Understanding the biofilm development of Acinetobacter baumannii and novel strategies to combat infection
    Naji Naseef Pathoor, Akshaya Viswanathan, Gulshan Wadhwa, Pitchaipillai Sankar Ganesh
    APMIS.2024; 132(5): 317.     CrossRef
  • PDT-Induced Variations of Radachlorin Fluorescence Lifetime in Living Cells In Vitro
    Andrey V. Belashov, Anna A. Zhikhoreva, Anna V. Salova, Tatiana N. Belyaeva, Ilia K. Litvinov, Elena S. Kornilova, Irina V. Semenova
    Photonics.2023; 10(11): 1262.     CrossRef

PHRP : Osong Public Health and Research Perspectives