Osong Public Health and Research Perspectives

Original Articles

Prevalence, multidrug resistance, and biofilm formation of Vibrio parahaemolyticus isolated from fish mariculture environments in Cat Ba Island, Vietnam: Kim Cuc Thi Nguyen, Phuc Hung Truong, Hoa Truong Thi, Xuan Tuy Ho, Phu Van Nguyen; Osong Public Health Res Perspect. 2024;15(1):56-67. Published online February 19, 2024; DOI: https://doi.org/10.24171/j.phrp.2023.0181

2,056 View
89 Download
1 Web of Science
2 Crossref

Objectives
Vibrio parahaemolyticus is a major foodborne pathogen in aquatic animals and a threat to human health worldwide. This study investigated the prevalence, antimicrobial resistance, antimicrobial resistance genes (ARGs), and biofilm formation of V. parahaemolyticus strains isolated from fish mariculture environments in Cat Ba Island, Vietnam. Methods: In total, 150 rearing water samples were collected from 10 fish mariculture farms in winter and summer. A polymerase chain reaction assay was used to identify V. parahaemolyticus, its virulence factors, and ARGs. The antimicrobial resistance patterns and biofilm formation ability of V. parahaemolyticus strains were investigated using the disk diffusion test and a microtiter plate-based crystal violet method, respectively. Results: Thirty-seven V. parahaemolyticus isolates were recovered from 150 samples. The frequencies of the tdh and trh genes among V. parahaemolyticus isolates were 8.1% and 21.6%, respectively. More than 90% of isolates were susceptible to ceftazidime, cefotaxime, and chloramphenicol, but over 72% were resistant to ampicillin, tetracycline, and erythromycin. Furthermore, 67.57% of isolates exhibited multidrug resistance. The presence of ARGs related to gentamicin (aac(3)-IV), tetracycline (tetA) and ciprofloxacin (qnrA) in V. parahaemolyticus isolates was identified. Conversely, no ARGs related to ampicillin or erythromycin resistance were detected. Biofilm formation capacity was detected in significantly more multidrug-resistant isolates (64.9%) than non-multidrug-resistant isolates (18.9%). Conclusion: Mariculture environments are a potential source of antibiotic-resistant V. parahaemolyticus and a hotspot for virulence genes and ARGs diffusing to aquatic environments. Thus, the prevention of antibiotic-resistant foodborne vibriosis in aquatic animals and humans requires continuous monitoring.

Citations

Citations to this article as recorded by

A New Strategy: Antibiotic Circumstances for Rational Drug use Against Clinical Aeromonas hydrophila And Bacterial Properties of This Bacteria on Different Agars
Nurdan Filik, Fethi Filik, Ayşegül Kubilay
Acta Aquatica Turcica.2024;[Epub] CrossRef
Passive immunoprophylaxis with Ccombodies against Vibrio parahaemolyticus in Pacific white shrimp (Penaeus vannamei)
Wanna Sirimanapong, Prawporn Thaijongrak, Chiranan Sudpraseart, Dennis Berbulla Bela-ong, Abigail Joy D. Rodelas-Angelia, Mark Rickard N. Angelia, Seungbeom Hong, Jaesung Kim, Kim D. Thompson, Tae Sung Jung
Fish & Shellfish Immunology.2024; 154: 109973. CrossRef

Prevalence of plasmid-mediated AmpC β-lactamases among uropathogenic Escherichia coli isolates in southwestern Iran: Nabi Jomehzadeh, Khadijeh Ahmadi, Zahra Rahmani; Osong Public Health Res Perspect. 2021;12(6):390-395. Published online December 1, 2021; DOI: https://doi.org/10.24171/j.phrp.2021.0272

6,094 View
112 Download
6 Web of Science
6 Crossref

Abstract PDF

Objectives
This study was undertaken to evaluate AmpC β-lactamase-producing Escherichia coli urine isolates and to characterize the frequency of plasmid-mediated AmpC (pAmpC)-encoding genes.
Methods
Antimicrobial susceptibility tests were performed using the disk diffusion technique. AmpC β-lactamase production was assessed with a phenotypic inhibitor-based method. The presence of 6 pAmpC-encoding cluster genes was detected by multiplex polymerase chain reaction (PCR).
Results
The proportion of antibiotic resistance of E. coli isolates ranged from 7.4% to 90.5%, and more than half (51.6%) of the total isolates were multidrug-resistant (MDR). Among the 95 E. coli isolates, 60 (63.2%) were found to be cefoxitin-resistant, but only 14 (14.7%) isolates were confirmed as AmpC β-lactamase-producers. In the PCR assay, pAmpC-encoding genes were found in 15 (15.8%) isolates, and blaDHA was the most prevalent type. However, blaFOX, blaMOX, and blaACC genes were not detected in the isolates.
Conclusion
Our findings contributed valuable information concerning antibiotic resistance, confirmatory phenotypic testing for AmpC production, and pAmpC β-lactamase gene content in E. coli isolates in southwestern Iran. The level of MDR recorded in AmpC-producing strains of this study was worrying; therefore, implementing strong infection control approaches to reduce the MDR burden is recommended.

Citations

Citations to this article as recorded by

A review of the mechanisms that confer antibiotic resistance in pathotypes of E. coli
Sina Nasrollahian, Jay P. Graham, Mehrdad Halaji
Frontiers in Cellular and Infection Microbiology.2024;[Epub] CrossRef
Association Between Uropathogenic Escherichia coli Virulence Genes and Severity of Infection and Resistance to Antibiotics
Sofía Alejandra Fonseca-Martínez, Ruth Aralí Martínez-Vega, Ana Elvira Farfán-García, Clara Isabel González Rugeles, Libeth Yajaira Criado-Guerrero
Infection and Drug Resistance.2023; Volume 16: 3707. CrossRef
Extended-spectrum β-lactamases producing Enterobacteriaceae (ESBL-PE) prevalence in Nepal: A systematic review and meta-analysis
Christina Khadka, Manita Shyaula, Gopiram Syangtan, Shrijana Bista, Reshma Tuladhar, Anjana Singh, Dev Raj Joshi, Lok R. Pokhrel, Prabin Dawadi
Science of The Total Environment.2023; 901: 166164. CrossRef
Prevalence of Extended-Spectrum β-Lactamase-Resistant Genes in Escherichia coli Isolates from Central China during 2016–2019
Zui Wang, Qin Lu, Xiaohui Mao, Li Li, Junfeng Dou, Qigai He, Huabin Shao, Qingping Luo
Animals.2022; 12(22): 3191. CrossRef
Molecular detection and characterization of Shigella spp. harboring extended-spectrum β-lactamase genes in children with diarrhea in northwest Iran
Sahar Sabour, Amir Teimourpour, Jafar Mohammadshahi, Hadi Peeridogaheh, Roghayeh Teimourpour, Taher Azimi, Zahra Hosseinali
Molecular and Cellular Pediatrics.2022;[Epub] CrossRef
Plasmid-mediated AmpC β-Lactamase Gene Analysis in Klebsiella Pneumoniae Clinical Isolates
Nabi Jomehzadeh, Khadijeh Ahmadi, Hasti Shaabaninejad, Gholamali Eslami
Biomedical and Biotechnology Research Journal (BBR.2022; 6(4): 582. CrossRef

Characterization of Antimicrobial Susceptibility, Extended-Spectrum β-Lactamase Genes and Phylogenetic Groups of Enteropathogenic Escherichia coli Isolated from Patients with Diarrhea: Erfaneh Jafari, Saeid Mostaan, Saeid Bouzari; Osong Public Health Res Perspect. 2020;11(5):327-333. Published online October 22, 2020; DOI: https://doi.org/10.24171/j.phrp.2020.11.5.09

8,920 View
121 Download
6 Web of Science
8 Crossref

Abstract PDF

Objectives

Infectious diarrhea is one of the most common causes of pediatric death worldwide and enteropathogenic Escherichia coli (EPEC) is one of the main causes. There are 2 subgroups of EPEC, typical and atypical, based on the presence or absence of bundle forming pili (bfp), of which atypical EPEC is considered less virulent, but not less pathogenic. Antimicrobial resistance towards atypical EPEC among children is growing and is considered a major problem. In this study the pattern of antibiotic resistance in clinical isolates was determined.

Methods

Using 130 isolates, antibiotic resistance patterns and phenotypes were assessed, and genotypic profiles of extended spectrum β-lactamase (ESBL) production using disc diffusion and PCR was carried out. Phylogenetic groups were analyzed using quadruplex PCR.

Results

There were 65 E. coli isolates identified as atypical EPEC by PCR, among which the highest antibiotic resistance was towards ampicillin, followed by trimethoprim-sulfamethoxazole, and tetracycline. Multidrug resistance was detected in 44.6% of atypical EPEC isolates. Around 33% of isolates were determined to be extended spectrum β-lactamase producers, and in 90% of isolates, genes responsible for ESBL production could be detected. Moreover, the majority of atypical EPEC strains belonged to Group E, followed by Groups B1, B2 and C.

Conclusion

High rates of multidrug resistance and ESBL production among atypical EPEC isolates warrant periodical surveillance studies to select effective antibiotic treatment for patients. It is considered a critical step to manage antibiotic resistance by avoiding unnecessary prescriptions for antibiotics.

Citations

Citations to this article as recorded by

Antibacterial Efficacy of Feline-Derived Lactic Acid Bacteria against Enteropathogenic Escherichia coli: A Comprehensive In Vitro Analysis
Weiwei Wang, Hao Dong, Qianqian Chen, Xiaohan Chang, Longjiao Wang, Chengyi Miao, Shuxing Chen, Lishui Chen, Ran Wang, Shaoyang Ge, Wei Xiong
Fermentation.2024; 10(10): 514. CrossRef
Phenotypic and molecular characterization of enteropathogenic Escherichia coli and Salmonella spp. causing childhood diarrhoea in Awka, South-Eastern Nigeria
Ifeanyi Emmanuel Nwike, Malachy Chigozie Ugwu, Peter Chika Ejikeugwu, Nonye Treasure Ujam, Ifeanyichukwu Romanus Iroha, Charles Okechukwu Esimone
Bulletin of the National Research Centre.2023;[Epub] CrossRef
Antimicrobial Resistance and Biofilm Formation of Escherichia coli Isolated from Pig Farms and Surroundings in Bulgaria
Mila D. Kaleva, Yana Ilieva, Maya Margaritova Zaharieva, Lyudmila Dimitrova, Tanya Chan Kim, Iva Tsvetkova, Yordan Georgiev, Petya Orozova, Krasimir Nedev, Hristo Najdenski
Microorganisms.2023; 11(8): 1909. CrossRef
Isolation and Molecular Characterization of Proteus mirabilis Bacteria from Poultry Meat in the Iraqi Market and a Study of their Resistance to some Antibiotics
Ammar H. Hamed, Ahmed I. Alnazzal
IOP Conference Series: Earth and Environmental Sci.2023; 1262(6): 062019. CrossRef
Virulence and phylogenetic analysis of enteric pathogenic Escherichia coli isolated from children with diarrhoea in South Africa
Ntando W. Alfinete, John Y. Bolukaoto, Lee Heine, Natasha Potgieter, Tobias G. Barnard
International Journal of Infectious Diseases.2022; 114: 226. CrossRef
Prevalence of extended-spectrum β-lactamases, AmpC, and carbapenemases in Proteus mirabilis clinical isolates
Mona Shaaban, Soha Lotfy Elshaer, Ola A. Abd El-Rahman
BMC Microbiology.2022;[Epub] CrossRef
Enteropathogenic Escherichia coli—A Summary of the Literature
Anca Delia Mare, Cristina Nicoleta Ciurea, Adrian Man, Bianca Tudor, Valeriu Moldovan, Luminița Decean, Felicia Toma
Gastroenterology Insights.2021; 12(1): 28. CrossRef
Prevalence of Antibiotic-Resistant Escherichia coli Isolated from Swine Faeces and Lagoons in Bulgaria
Lyudmila Dimitrova, Mila Kaleva, Maya M. Zaharieva, Christina Stoykova, Iva Tsvetkova, Maya Angelovska, Yana Ilieva, Vesselin Kussovski, Sevda Naydenska, Hristo Najdenski
Antibiotics.2021; 10(8): 940. CrossRef

High Prevalence of Class 1 to 3 Integrons Among Multidrug-Resistant Diarrheagenic Escherichia coli in Southwest of Iran: Mohammad Kargar, Zahra Mohammadalipour, Abbas Doosti, Shahrokh Lorzadeh, Alireza Japoni-Nejad; Osong Public Health Res Perspect. 2014;5(4):193-198. Published online August 31, 2014; DOI: https://doi.org/10.1016/j.phrp.2014.06.003

3,878 View
30 Download
45 Crossref

Abstract PDF

Objectives
Horizontal transfer of integrons is one of the important factors that can contribute to the occurrence of multidrug-resistant (MDR) bacteria. This study aimed to determine the prevalence of integrons among MDR Escherichia coli strains isolated from stool specimens and investigate the associations between the existence of integrons and MDR properties in the southwest of Iran.
Methods
There were 164 E. coli strains isolated from January 2012 to June 2012. Fecal specimens identified as E. coli by the conventional methods. Subsequently the antibiotic resistance was assessed using Clinical and Laboratory Standard Institute criteria. The presence of class 1–3 integrons and embedded gene cassettes was verified using specific primers by multiplex polymerase chain reaction assay.
Results
Among a total of 164 studied samples, 69 (42.07%) isolates were multidrug resistant. Class 1 and class 2 integrons were present in 78.26% and 76.81% MDR isolates, respectively. For the first time in Iran, class 3 integron was observed in 26.09% MDR isolates. Significant correlations were identified between: class 1 integron and resistance to amikacin, gentamicin, chloramphenicol, ampicillin, tetracycline, nalidixic acid, and co-trimoxazole; class 2 integron and resistance to aminoglycosides, co-trimoxazole, cefalexin, ampicillin, and chloramphenicol; and class 3 integron and resistance to gentamicin, kanamycin, and streptomycin.
Conclusion
Our results indicate that integrons are common among MDR isolates and they can be used as a marker for the identification of MDR isolates. Therefore, due to the possibility of a widespread outbreak of MDR isolates, molecular surveillance and sequencing of the integrons in other parts of the country is recommended.

Citations

Citations to this article as recorded by

Municipal wastewater treatment plant showing a potential reservoir for clinically relevant MDR bacterial strains co-occurrence of ESBL genes and integron-integrase genes
Kuldeep Soni, David Kothamasi, Ram Chandra
Journal of Environmental Management.2024; 351: 119938. CrossRef
Ciprofloxacin and Imipenem Resistance in Bathing Waters—Preliminary Studies of Great Rudnickie Lake
Natalia Jendrzejewska, Ewa Karwowska
Applied Sciences.2024; 14(14): 6238. CrossRef
Detection of virulence factor genes, antibiotic resistance genes and biofilm formation in clinical Gram-negative bacteria and first report from Türkiye of K.oxytoca carrying both blaOXA-23 and blaOXA-51 genes
Azer Özad Düzgün, Gamze Yüksel
Biologia.2023; 78(8): 2245. CrossRef
Antimicrobial Resistance in Romania: Updates on Gram-Negative ESCAPE Pathogens in the Clinical, Veterinary, and Aquatic Sectors
Ilda Czobor Barbu, Irina Gheorghe-Barbu, Georgiana Alexandra Grigore, Corneliu Ovidiu Vrancianu, Mariana Carmen Chifiriuc
International Journal of Molecular Sciences.2023; 24(9): 7892. CrossRef
Extended-Spectrum β-Lactamase-Producing Escherichia coli Isolated from Food-Producing Animals in Tamaulipas, Mexico
Antonio Mandujano, Diana Verónica Cortés-Espinosa, José Vásquez-Villanueva, Paulina Guel, Gildardo Rivera, Karina Juárez-Rendón, Wendy Lizeth Cruz-Pulido, Guadalupe Aguilera-Arreola, Abraham Guerrero, Virgilio Bocanegra-García, Ana Verónica Martínez-Vázqu
Antibiotics.2023; 12(6): 1010. CrossRef
Antimicrobial susceptibility and integrons detection among extended-spectrum β-lactamase producing Enterobacteriaceae isolates in patients with urinary tract infection
Karzan Taha Abubaker, Khanda Abdulateef Anwar
PeerJ.2023; 11: e15429. CrossRef
Brucella abortus antigen omp25 vaccines: Development and targeting based on Lactococcus lactis
Somaye Tirbakhsh Gouran, Abbas Doosti, Mohammad Saeid Jami
Veterinary Medicine and Science.2023; 9(4): 1908. CrossRef
Integrons in the development of antimicrobial resistance: critical review and perspectives
Basharat Ahmad Bhat, Rakeeb Ahmad Mir, Hafsa Qadri, Rohan Dhiman, Abdullah Almilaibary, Mustfa Alkhanani, Manzoor Ahmad Mir
Frontiers in Microbiology.2023;[Epub] CrossRef
Tracing the Evolutionary Pathways of Serogroup O78 Avian Pathogenic Escherichia coli
Eun-Jin Ha, Seung-Min Hong, Seung-Ji Kim, Sun-Min Ahn, Ho-Won Kim, Kang-Seuk Choi, Hyuk-Joon Kwon
Antibiotics.2023; 12(12): 1714. CrossRef
Molecular Detection of Integrons, Colistin and β-lactamase Resistant Genes in Salmonella enterica Serovars Enteritidis and Typhimurium Isolated from Chickens and Rats Inhabiting Poultry Farms
Tsepo Ramatla, Kealeboga Mileng, Rendani Ndou, Nthabiseng Mphuti, Michelo Syakalima, Kgaugelo E. Lekota, Oriel M.M. Thekisoe
Microorganisms.2022; 10(2): 313. CrossRef
Prevalence of the Integrons and ESBL Genes in Multidrug-Resistant Strains of Escherichia coli Isolated from Urinary Tract Infections, Ardabil, Iran
Soheyla Barzegar, Mohsen Arzanlou, Amir Teimourpour, Majid Esmaelizad, Mehdi Yousefipour, Jafar MohammadShahi, Roghayeh Teimourpour
Iranian Journal of Medical Microbiology.2022; 16(1): 56. CrossRef
Migration of antibiotic resistance genes and evolution of flora structure in the Xenopus tropicalis intestinal tract with combined exposure to roxithromycin and oxytetracycline
Xiaojun Lin, Yanbin Xu, Ruiqi Han, Wenshi Luo, Li Zheng
Science of The Total Environment.2022; 820: 153176. CrossRef
Investigation of class 1 integrons and biofilm formation in multi-drug resistance uropathogenic Escherichia coli isolated from patients with urinary tract infection in Shohadaye Qom hospital, Iran
Ahmad Khorshidi, NadiaMohammad Zadeh, Azad Khaledi, GholamAbbas Moosavi, Ali Shakerimoghaddam, Azade Matinpur
International Archives of Health Sciences.2022; 9(1): 47. CrossRef
Prevalence and characterisation of antimicrobial resistance genes and class 1 and 2 integrons in multiresistant Escherichia coli isolated from poultry production
Przemysław Racewicz, Michał Majewski, Hanna Biesiada, Sebastian Nowaczewski, Jarosław Wilczyński, Danuta Wystalska, Magdalena Kubiak, Marcin Pszczoła, Zofia E. Madeja
Scientific Reports.2022;[Epub] CrossRef
Prevalence of integrons in multidrug-resistant Escherichia coli isolates from waters and vegetables in Nsukka and Enugu, Southeast Nigeria
Chinyere B. Chigor, Ini-Abasi I. Ibangha, Nkechinyere O. Nweze, Valentino C. Onuora, Chizoba A. Ozochi, Yinka Titilawo, Matthew C. Enebe, Tatyana N. Chernikova, Peter N. Golyshin, Vincent N. Chigor
Environmental Science and Pollution Research.2022; 29(40): 60945. CrossRef
Antimicrobial resistance and genetic diversity of Staphylococcus aureus collected from livestock, poultry and humans
Sangeeta Rao, Lyndsey Linke, Roberta Magnuson, Linzy Jauch, Doreene R. Hyatt
One Health.2022; 15: 100407. CrossRef
The Emergence of Carbapenem-Resistant Gram-Negative Bacteria in Mizoram, Northeast India
Vanlalruati S. C. Ralte, Archana Loganathan, Prasanth Manohar, Christine Vanlalbiakdiki Sailo, Zothan Sanga, Lalremruata Ralte, John Zothanzama, Sebastian Leptihn, Ramesh Nachimuthu, Nachimuthu Senthil Kumar
Microbiology Research.2022; 13(3): 342. CrossRef
Common Etiological Agents in Adult Patients with Gastroenteritis from Central Iran
Elnaz Abbasi, Alex van Belkum, Ehsanollah Ghaznavi-Rad
Microbial Drug Resistance.2022; 28(11): 1043. CrossRef
Antibiotic Resistance in Proteus mirabilis: Mechanism, Status, and Public Health Significance
Ebtehal Alqurashi, Khaled Elbanna, Iqbal Ahmad, Hussein H. Abulreesh
Journal of Pure and Applied Microbiology.2022; 16(3): 1550. CrossRef
Antibiotic Susceptibility Profiles and Resistance Mechanisms to β-Lactams and Polymyxins of Escherichia coli from Broilers Raised under Intensive and Extensive Production Systems
Mariana Ferreira, Célia Leão, Lurdes Clemente, Teresa Albuquerque, Ana Amaro
Microorganisms.2022; 10(10): 2044. CrossRef
Antimicrobial Resistance, Integron Carriage, and Fluoroquinolone Resistance Genes in Acinetobacte baumannii Isolates
Parastoo Ashouri, Jafar Mohammadshahi, Vajihe Sadat Nikbin, Hadi Peeridogaheh, Behnam Mohammadi-Ghalehbin, Soheila Refahi, Amir Teimourpour, Majid Esmaelizad, Hafez Mirzaneghad, Roghayeh Teimourpour
Archives of Clinical Infectious Diseases.2022;[Epub] CrossRef
Multidrug resistance-encoding gene in Citrobacter freundii isolated from healthy laying chicken in Blitar District, Indonesia
Adiana Mutamsari Witaningrum, Freshinta Jellia Wibisono, Dian Ayu Permatasari, Mustofa Helmi Effendi, Emmanuel Nnabuike Ugbo
International Journal of One Health.2022; : 161. CrossRef
Evaluation of Retail Meat as a Source of ESBL Escherichia coli in Tamaulipas, Mexico
Ana Verónica Martínez-Vázquez, Antonio Mandujano, Eduardo Cruz-Gonzalez, Abraham Guerrero, Jose Vazquez, Wendy Lizeth Cruz-Pulido, Gildardo Rivera, Virgilio Bocanegra-García
Antibiotics.2022; 11(12): 1795. CrossRef
Characterization of Integrons and Quinolone Resistance in Clinical Escherichia coli Isolates in Mansoura City, Egypt
Shaymaa H. Abdel-Rhman, Rehab M. Elbargisy, Dina E. Rizk, Ahmed Majeed Al-Shammari
International Journal of Microbiology.2021; 2021: 1. CrossRef
Prevalence and Characterization of ESBL/AmpC Producing Escherichia coli from Fresh Meat in Portugal
Lurdes Clemente, Célia Leão, Laura Moura, Teresa Albuquerque, Ana Amaro
Antibiotics.2021; 10(11): 1333. CrossRef
Association of phylogenetic distribution and presence of integrons with multidrug resistance in Escherichia coli clinical isolates from children with diarrhoea
Yesmi Patricia Ahumada-Santos, María Elena Báez-Flores, Sylvia Páz Díaz-Camacho, Magdalena de Jesús Uribe-Beltrán, Carlos Alberto Eslava-Campos, Jesús Ricardo Parra-Unda, Francisco Delgado-Vargas
Journal of Infection and Public Health.2020; 13(5): 767. CrossRef
Class 1 Integrons in Clinical Multi Drug Resistance E. coli, Sana’a Hospitals, Yemen
Mukhtar A. Al-Hammadi, Hassan A. Al-Shamahy, Abdulaziz Q. Ali, Mahfoudh A.M. Abdulghani, Hassan Pyar, Ibrahim AL-Suboal
Pakistan Journal of Biological Sciences.2020; 23(3): 231. CrossRef
Occurrence, Phenotypic and Molecular Characterization of Extended-Spectrum- and AmpC- β-Lactamase Producing Enterobacteriaceae Isolated From Selected Commercial Spinach Supply Chains in South Africa
Loandi Richter, Erika M. du Plessis, Stacey Duvenage, Lise Korsten
Frontiers in Microbiology.2020;[Epub] CrossRef
Staphylococcus aureus biofilms: Structures, antibiotic resistance, inhibition, and vaccines
Raziey Parastan, Mohammad Kargar, Kavous Solhjoo, Farshid Kafilzadeh
Gene Reports.2020; 20: 100739. CrossRef
Dissemination of antibiotic resistance genes (ARGs) via integrons in Escherichia coli: A risk to human health
Shaqiu Zhang, Muhammad Abbas, Mujeeb Ur Rehman, Yahui Huang, Rui Zhou, Siyue Gong, Hong Yang, Shuling Chen, Mingshu Wang, Anchun Cheng
Environmental Pollution.2020; 266: 115260. CrossRef
Multi-Drug-Resistant Diarrheagenic Escherichia coli Pathotypes in Pediatric Patients with Gastroenteritis from Central Iran

Elnaz Abbasi, Mahdieh mondanizadeh, Alex van Belkum, Ehsanollah Ghaznavi-Rad
Infection and Drug Resistance.2020; Volume 13: 1387. CrossRef
The Relationship of Class I Integron Gene Cassettes and the Multidrug-Resistance in Extended -Spectrum β-Lactamase Producing Isolates of Escherichia coli
Alisha Akya, Roya Chegene Lorestani, Mosayeb Rostamian, Azam Elahi, Shokofe Baakhshii, Minoo Aliabadi, Keyghobad Ghadiri
Archives of Pediatric Infectious Diseases.2019;[Epub] CrossRef
Integrons in Enterobacteriaceae : diversity, distribution and epidemiology
Megha Kaushik, Sanjay Kumar, Rajeev Kumar Kapoor, Jugsharan Singh Virdi, Pooja Gulati
International Journal of Antimicrobial Agents.2018; 51(2): 167. CrossRef
Molecular analysis of Shiga toxin-producing Escherichia coli O157:H7 and non-O157 strains isolated from calves
Maryam Kohansal, Ali Ghanbari Asad
Onderstepoort Journal of Veterinary Research.2018;[Epub] CrossRef
Prevalence of Integrons and Insertion Sequences in ESBL-Producing E. coli Isolated from Different Sources in Navarra, Spain
Lara Pérez-Etayo, Melibea Berzosa, David González, Ana Isabel Vitas
International Journal of Environmental Research an.2018; 15(10): 2308. CrossRef
Frequency of antimicrobial resistance and integron gene cassettes in Escherichia coli isolated from giant pandas (Ailuropoda melanoleuca) in China
Wencheng Zou, Caiwu Li, Xin Yang, Yongxiang Wang, Guangyang Cheng, Jinxin Zeng, Xiuzhong Zhang, Yanpeng Chen, Run Cai, Qianru Huang, Lan Feng, Hongning Wang, Desheng Li, Guiquan Zhang, Yanxi Chen, Zhizhong Zhang, Heming Zhang
Microbial Pathogenesis.2018; 116: 173. CrossRef
Colistin-Resistant Klebsiella pneumoniae: Prevalence of Integrons and Synergistic Out Turn for Colistin-Meropenem
Prasanth Manohar, Thamaraiselvan Shanthini, Pandey Ekta, Mahesan J B, Kodiveri Muthukaliannan Gothandam, Bulent Bozdogan, Nachimuthu Ramesh
Archives of Clinical Infectious Diseases.2018;[Epub] CrossRef
Evaluation of integrons classes 1–3 in extended spectrum beta-lactamases and multi drug resistant Escherichia coli isolates in the North of Iran
Shahla Asgharzadeh Kangachar, Ali Mojtahedi
Gene Reports.2018; 12: 299. CrossRef
Association of Glycerol Kinase Gene with Class 3 Integrons: A Novel Cassette Array within Escherichia coli
Rajkumari Elizabeth, Debadatta Dhar Chanda, Atanu Chakravarty, Deepjyoti Paul, Shiela Chetri, Deepshikha Bhowmik, Jayalaxmi Wangkheimayum, Amitabha Bhattacharjee
Indian Journal of Medical Microbiology.2018; 36(1): 104. CrossRef
Emergence of class 1 to 3 integrons among members of Enterobacteriaceae in Egypt
Dina E. Rizk, Areej M. El-Mahdy
Microbial Pathogenesis.2017; 112: 50. CrossRef
Detection of Class I and II integrons for the assessment of antibiotic and multidrug resistance amongEscherichia coliisolates from agricultural irrigation waters in Bulacan, Philippines
Cielo Emar M. Paraoan, Windell L. Rivera, Pierangeli G. Vital
Journal of Environmental Science and Health, Part .2017; 52(5): 306. CrossRef
Distribution of Integrons and Phylogenetic Groups among Enteropathogenic Escherichia coli Isolates from Children <5 Years of Age in Delhi, India
Taru Singh, Shukla Das, V. G. Ramachandran, Sayim Wani, Dheeraj Shah, Khan A. Maroof, Aditi Sharma
Frontiers in Microbiology.2017;[Epub] CrossRef
The distribution of carbapenem- and colistin-resistance in Gram-negative bacteria from the Tamil Nadu region in India
Prasanth Manohar, Thamaraiselvan Shanthini, Ramankannan Ayyanar, Bulent Bozdogan, Aruni Wilson, Ashok J. Tamhankar, Ramesh Nachimuthu, Bruno S. Lopes
Journal of Medical Microbiology .2017; 66(7): 874. CrossRef
Multidrug-Resistant Escherichia coli Strains Isolated from Patients Are Associated with Class 1 and 2 Integrons
Hamid Lavakhamseh, Parviz Mohajeri, Samaneh Rouhi, Pegah Shakib, Rashid Ramazanzadeh, Afshin Rasani, Majid Mansouri
Chemotherapy.2016; 61(2): 72. CrossRef
Variability in gene cassette patterns of class 1 and 2 integrons associated with multi drug resistance patterns in Staphylococcus aureus clinical isolates in Tehran-Iran
Mahdi Mostafa, Seyed Davar Siadat, Fereshteh Shahcheraghi, Farzam Vaziri, Alireza Japoni-Nejad, Jalil Vand Yousefi, Bahareh Rajaei, Elnaz Harifi Mood, Nayyereh Ebrahim zadeh, Arfa Moshiri, Seyed Alireza Seyed Siamdoust, Mohamad Rahbar
BMC Microbiology.2015;[Epub] CrossRef

In Vitro Antibacterial Efficacy of 21 Indian Timber-Yielding Plants Against Multidrug-Resistant Bacteria Causing Urinary Tract Infection: Monali P. Mishra, Rabindra N. Padhy; Osong Public Health Res Perspect. 2013;4(6):347-357. Published online December 31, 2013; DOI: https://doi.org/10.1016/j.phrp.2013.10.007

3,831 View
25 Download
22 Crossref

Abstract PDF

Objectives
To screen methanolic leaf extracts of 21 timber-yielding plants for antibacterial activity against nine species of uropathogenic bacteria isolated from clinical samples of a hospital (Enterococcus faecalis, Staphylococcus aureus, Acinetobacter baumannii, Citrobacter freundii, Enterobacter aerogenes, Escherichia coli, Klebsiella pneumoniae, Proteus mirabilis, and Pseudomonas aeruginosa).
Methods
Bacterial strains were subjected to antibiotic sensitivity tests by the Kirby–Bauer's disc diffusion method. The antibacterial potentiality of leaf extracts was monitored by the agar-well diffusion method with multidrug-resistant (MDR) strains of nine uropathogens.
Results
Two Gram-positive isolates, E. faecalis and S. aureus, were resistant to 14 of the 18 antibiotics used. Gram-negative isolates A. baumannii, C. freundii, E. aerogenes, E. coli, K. pneumoniae, P. mirabilis, and P. aeruginosa were resistant to 10, 12, 9, 11, 11, 10, and 11 antibiotics, respectively, of the 14 antibiotics used. Methanolic leaf extracts of Anogeissus acuminata had the maximum zone of inhibition size—29 mm against S. aureus and 28 mm against E. faecalis and P. aeruginosa. Cassia tora had 29 mm as the zone of inhibition size for E. faecalis, E. aerogenes, and P. aeruginosa. Based on the minimum inhibitory concentration and minimum bactericidal concentration values, the most effective 10 plants against uropathogens could be arranged in decreasing order as follows: C. tora > A. acuminata > Schleichera oleosa > Pterocarpus santalinus > Eugenia jambolana > Bridelia retusa > Mimusops elengi > Stereospermum kunthianum > Tectona grandis > Anthocephalus cadamba. The following eight plants had moderate control capacity: Artocarpus heterophyllus, Azadirachta indica, Dalbergia latifolia, Eucalyptus citriodora, Gmelina arborea, Pongamia pinnata, Pterocarpus marsupium, and Shorea robusta. E. coli, followed by A. baumannii, C. freundii, E. aerogenes, P. mirabilis, and P. aeruginosa were controlled by higher amounts/levels of leaf extracts. Phytochemicals of all plants were qualitatively estimated.
Conclusions
A majority of timber-yielding plants studied had in vitro control capacity against MDR uropathogenic bacteria.

Citations

Citations to this article as recorded by

Diabetes Warriors from Heart Wood: Unveiling Dalbergin and Isoliquiritigenin from Dalbergia latifolia as Potential Antidiabetic Agents in-vitro and in-vivo
Srinivas Sutrapu, Rashmi Saxena Pal, Navneet Khurana, Harish Vancha, Sharfuddin Mohd, Krishna Mohan Chinnala, Bimlesh Kumar, Govindaiah Pilli
Cell Biochemistry and Biophysics.2024; 82(2): 1309. CrossRef
Ethnopharmacological survey of medicinal plants used by the Nyambo and Haya people of Kyerwa district in northwestern Tanzania to treat urinary tract infections
Judith Costantine, Rogers Mwakalukwa, Deborah Runyoro, Godfrey Sambayi, Cleopatra Justine, Meshack Lugoba
Clinical Traditional Medicine and Pharmacology.2024; 5(4): 200177. CrossRef
Chemical constituents, pharmacological activities, and uses of common ayurvedic medicinal plants: a future source of new drugs
Parul Kaushik, Priyanka Ahlawat, Kuldeep Singh, Raman Singh
Advances in Traditional Medicine.2023; 23(3): 673. CrossRef
Antibacterial activity of Libyan Juniperus phoenicea L. leaves extracts against common nosocomial pathogens
Aml O. Alhadad, Galal S. Salem, Suliman M. Hussein, Sarah M. Elshareef
Journal of Experimental Biology and Agricultural S.2023; 11(2): 371. CrossRef
The Ethnopharmacology, Phytochemistry and Bioactivities of the Corymbia Genus (Myrtaceae)
Matthew J. Perry, Phurpa Wangchuk
Plants.2023; 12(21): 3686. CrossRef
Gas Chromatography-Mass Spectroscopic, high performance liquid chromatographic and In-silico characterization of antimicrobial and antioxidant constituents of Rhus longipes(Engl)
Adedoyin Adetutu Olasunkanmi, Olumide Samuel Fadahunsi, Peter Ifeoluwa Adegbola
Arabian Journal of Chemistry.2022; 15(2): 103601. CrossRef
Evaluation of the sensory attributes of pepper soup beef hides and determination of the preservative potential of the spices used for its preparation
T.C.L. Maguipa, P.D. Mbougueng, H.M. Womeni
Journal of Agriculture and Food Research.2022; 8: 100293. CrossRef
Flourensia retinophylla: An outstanding plant from northern Mexico with antibacterial activity
D. Jasso de Rodríguez, M.C. Victorino-Jasso, N.E. Rocha-Guzmán, M.R. Moreno-Jiménez, L. Díaz-Jiménez, R. Rodríguez-García, J.Á. Villarreal-Quintanilla, F.M. Peña-Ramos, D.A. Carrillo-Lomelí, Z.A. Genisheva, M.L. Flores-López
Industrial Crops and Products.2022; 185: 115120. CrossRef
Antibacterial Screening, Biochemometric and Bioautographic Evaluation of the Non-Volatile Bioactive Components of Three Indigenous South African Salvia Species
Margaux Lim Ah Tock, Sandra Combrinck, Guy Kamatou, Weiyang Chen, Sandy Van Vuuren, Alvaro Viljoen
Antibiotics.2022; 11(7): 901. CrossRef
Computational in Silico Modelling of Phytochemicals as a Potential Cure
Rachita Kurmi, Kavya N R, Jennath Sherin A, Silpa T S
International Journal of Scientific Research in Sc.2021; : 42. CrossRef
Hepatoprotective effects of Cassiae Semen on mice with non-alcoholic fatty liver disease based on gut microbiota
Hanyan Luo, Hongwei Wu, Lixia Wang, Shuiming Xiao, Yaqi Lu, Cong Liu, Xiankuo Yu, Xiao Zhang, Zhuju Wang, Liying Tang
Communications Biology.2021;[Epub] CrossRef
Phytochemical screening, anthocyanins and antimicrobial activities in some berries fruits
Amina A. Aly, Hoda G. M. Ali, Noha E. R. Eliwa
Journal of Food Measurement and Characterization.2019; 13(2): 911. CrossRef
Antimicrobial activity of select edible plants from Odisha, India against food-borne pathogens
Sujogya Kumar Panda, Yugal Kishore Mohanta, Laxmipriya Padhi, Walter Luyten
LWT.2019; 113: 108246. CrossRef
Extracts of Tectona grandis and Vernonia amygdalina have anti-Toxoplasma and pro-inflammatory properties in vitro
Mlatovi Dégbé, Françoise Debierre-Grockiego, Amivi Tété-Bénissan, Héloïse Débare, Kodjo Aklikokou, Isabelle Dimier-Poisson, Messanvi Gbeassor
Parasite.2018; 25: 11. CrossRef
Psacalium paucicapitatum has in vitro antibacterial activity
D. Jasso de Rodríguez, L.C. García-Hernández, N.E. Rocha-Guzmán, M.R. Moreno-Jiménez, R. Rodríguez-García, M.L.V. Díaz-Jiménez, A. Sáenz-Galindo, J.A. Villarreal-Quintanilla, F.M. Peña-Ramos, M.L. Flores-López, D.A. Carrillo-Lomelí
Industrial Crops and Products.2017; 107: 489. CrossRef
Qualitative Phytochemical Analysis, Antimicrobial Activity and Cytotoxic Effect of Moringa concanensis Nimmo Leaves
Ramaswamy Malathi, Solaimuthu Chandrasek
Research Journal of Medicinal Plants.2017; 11(3): 93. CrossRef
In vitro antibacterial activity of crude extracts of 9 selected medicinal plants against UTI causing MDR bacteria
Monali P. Mishra, Sibanarayan Rath, Shasank S. Swain, Goutam Ghosh, Debajyoti Das, Rabindra N. Padhy
Journal of King Saud University - Science.2017; 29(1): 84. CrossRef
Phytochemical investigation and antimicrobial assessment of Bellis sylvestris leaves
Monica Scognamiglio, Elisabetta Buommino, Lorena Coretti, Vittoria Graziani, Rosita Russo, Pina Caputo, Giovanna Donnarumma, Brigida D⿿Abrosca, Antonio Fiorentino
Phytochemistry Letters.2016; 17: 6. CrossRef
The genus Anogeissus: A review on ethnopharmacology, phytochemistry and pharmacology
Deeksha Singh, Uttam Singh Baghel, Anshoo Gautam, Dheeraj Singh Baghel, Divya Yadav, Jai Malik, Rakesh Yadav
Journal of Ethnopharmacology.2016; 194: 30. CrossRef
Antibacterial activity of five Peruvian medicinal plants against Pseudomonas aeruginosa
Gabriela Ulloa-Urizar, Miguel Angel Aguilar-Luis, María del Carmen De Lama-Odría, José Camarena-Lizarzaburu, Juana del Valle Mendoza
Asian Pacific Journal of Tropical Biomedicine.2015; 5(11): 928. CrossRef
In vitro antibacterial efficacy of plants used by an Indian aboriginal tribe against pathogenic bacteria isolated from clinical samples
Shasank S. Swain, Rabindra N. Padhy
Journal of Taibah University Medical Sciences.2015; 10(4): 379. CrossRef
Monitoring in vitro antibacterial efficacy of 26 Indian spices against multidrug resistant urinary tract infecting bacteria
Sibanarayan Rath, Rabindra N. Padhy
Integrative Medicine Research.2014; 3(3): 133. CrossRef

Article

Comparison of Antimicrobial Resistance in Escherichia coli Strains Isolated From Healthy Poultry and Swine Farm Workers Using Antibiotics in Korea: Seung-Hak Cho, Yeong-Sik Lim, Yeon-Ho Kang; Osong Public Health Res Perspect. 2012;3(3):151-155. Published online June 30, 2012; DOI: https://doi.org/10.1016/j.phrp.2012.07.002

3,465 View
24 Download
22 Crossref

Abstract PDF

Objectives
The aim of this study is to compare the antibiotic resistance of Escherichia coli isolates from faecal samples of workers who often use antibiotics.
Methods
A total of 163E coli strains isolated from faecal samples of livestock workers (poultry and swine farm workers) and restaurant workers in the same regions as a control group were analyzed by agar disc diffusion to determine their susceptibility patterns to 16 antimicrobial agents.
Results
Most of the tested isolates showed high antimicrobial resistance to ampicillin and tetracycline. The isolates showed higher resistance to cephalothin than other antibiotics among the cephems. Among the aminoglycosides, the resistance to gentamicin and tobramycin occurred at higher frequencies compared with resistance to amikacin and netilmicin. Our data indicated that faecal E coli isolates of livestock workers showed higher antibiotic resistances than nonlivestock workers (restaurant workers), especially cephalothin, gentamicin, and tobramycin (p < 0.05). Moreover, the rates of the livestock workers in the association of multidrug resistance were also higher than the rates of the restaurant workers.
Conclusion
This study implies that usage of antibiotics may contribute to the prevalence of antibiotic resistance in commensal E coli strains of humans.

Citations

Citations to this article as recorded by

Comparison of Antimicrobial Resistance in Escherichia coli Strains Isolated From Swine, Poultry, and Farm Workers in the Respective Livestock Farming Units in Greece
Magdalini K Christodoulou
Cureus.2023;[Epub] CrossRef
Risk Factors Associated with the Carriage of Pathogenic Escherichia coli in Healthy Commercial Meat Chickens in Queensland, Australia †
Leena Awawdeh, Rachel Forrest, Conny Turni, Rowland Cobbold, Joerg Henning, Justine Gibson
Poultry.2022; 1(2): 94. CrossRef
Characteristics and nutrient function of intestinal bacterial communities in black soldier fly (Hermetia illucens L.) larvae in livestock manure conversion
Yue Ao, Chongrui Yang, Shengchen Wang, Qingyi Hu, Li Yi, Jibin Zhang, Ziniu Yu, Minmin Cai, Chan Yu
Microbial Biotechnology.2021; 14(3): 886. CrossRef
Insects, Rodents, and Pets as Reservoirs, Vectors, and Sentinels of Antimicrobial Resistance
Willis Gwenzi, Nhamo Chaukura, Norah Muisa-Zikali, Charles Teta, Tendai Musvuugwa, Piotr Rzymski, Akebe Luther King Abia
Antibiotics.2021; 10(1): 68. CrossRef
Phenotypic and genotypic antimicrobial resistance patterns of Escherichia coli and Klebsiella isolated from dairy farm milk, farm slurry and water in Punjab, India
Prateek Jindal, Jasbir Bedi, Randhir Singh, Rabinder Aulakh, Jatinder Gill
Environmental Science and Pollution Research.2021; 28(22): 28556. CrossRef
Analysis of drug sensitivity of Escherichia Coli O157H7
Minzi Xu, Zhenyu Liu, Yanbo Song, Runan Zhao, Zheng Yang, Huijin Zhao, Xiaobing Sun, Yaning Gu, Huifei Yang
Biomedical Microdevices.2021;[Epub] CrossRef
One Health of Peripheries: Biopolitics, Social Determination, and Field of Praxis
Oswaldo Santos Baquero
Frontiers in Public Health.2021;[Epub] CrossRef
Antimicrobial resistance in fecal Escherichia coli isolated from poultry chicks in northern Iran
Zohreh Pourhossein, Leila Asadpour, Hadi Habibollahi, Seyedeh Tooba Shafighi
Gene Reports.2020; 21: 100926. CrossRef
Prevalence of Multidrug Resistant Escherichia Coli In Suspected Cases of Urinary Tract Infection Among Patients Attending Ahmadu Bello University Medical Center, Zaria
Shitu, S., Gambo, B. A., Musa, M.O., Abubakar, A.A., Attahiru, M.
UMYU Journal of Microbiology Research (UJMR).2020; 5(2): 123. CrossRef
Prevalence and risk factors for multi-drug resistant Escherichia coli among poultry workers in the Federal Capital Territory, Abuja, Nigeria
Mabel Kamweli Aworh, Jacob Kwaga, Emmanuel Okolocha, Nwando Mba, Siddhartha Thakur, Grzegorz Woźniakowski
PLOS ONE.2019; 14(11): e0225379. CrossRef
High genomic diversity of multi-drug resistant wastewater Escherichia coli
Norhan Mahfouz, Serena Caucci, Eric Achatz, Torsten Semmler, Sebastian Guenther, Thomas U. Berendonk, Michael Schroeder
Scientific Reports.2018;[Epub] CrossRef
Characterization of Escherichia coli Isolated from Day-old Chicken Fluff in Taiwanese Hatcheries
Shengnan Zhao, Chia-Lan Wang, Shao-Kuang Chang, Yi-Lun Tsai, Chung-Hsi Chou
Avian Diseases.2018; 63(1): 9. CrossRef
Changes in antimicrobial resistance patterns and dominance of extended spectrum β-lactamase genes among faecal Escherichia coli isolates from broilers and workers during two rearing periods
Fatemeh Doregiraee, Masoud Alebouyeh, Bahar Nayeri Fasaei, Saeed Charkhkar, Elahe Tajeddin, Mohammad Reza Zali
Italian Journal of Animal Science.2018; 17(3): 815. CrossRef
Oxytetracycline reduces the diversity of tetracycline-resistance genes in the Galleria mellonella gut microbiome
Katarzyna Ignasiak, Anthony Maxwell
BMC Microbiology.2018;[Epub] CrossRef
Antimicrobial Resistance and the Presence of Virulence Genes in Escherichia coli Strains Isolated from Ruditapes philippinarum in Gomso Bay, Korea
Tae-Ok Kim, In-Seon Eom, Kwang-Ho Park, Kwon-Sam Park
Korean Journal of Fisheries and Aquatic Sciences.2016; 49(6): 800. CrossRef
High prevalence of cross-resistance to fluoroquinolone and cotrimoxazole in tetracycline-resistant Escherichia coli human clinical isolates
Eric Batard, Mathilde Lefebvre, Guillaume Ghislain Aubin, Nathalie Caroff, Stéphane Corvec
Journal of Chemotherapy.2016; 28(6): 510. CrossRef
Soil‐borne reservoirs of antibiotic‐resistant bacteria are established following therapeutic treatment of dairy calves
Jinxin Liu, Zhe Zhao, Lisa Orfe, Murugan Subbiah, Douglas R. Call
Environmental Microbiology.2016; 18(2): 557. CrossRef
Antibiotic Resistance in Airborne Bacteria Near Conventional and Organic Beef Cattle Farms in California, USA
Helen M. Sancheza, Cristina Echeverria, Vanessa Thulsiraj, Amy Zimmer-Faust, Ariel Flores, Madeleine Laitz, Gregory Healy, Shaily Mahendra, Suzanne E. Paulson, Yifang Zhu, Jennifer A. Jay
Water, Air, & Soil Pollution.2016;[Epub] CrossRef
Transport of Antibiotic Resistance Plasmids in Porous Media
Chaoqi Chen, Jing Li, Stephanie L. DeVries, Pengfei Zhang, Xiqing Li
Vadose Zone Journal.2015; 14(3): 1. CrossRef
Possibility of CTX-M-14 Gene Transfer from Shigella sonnei to a Commensal Escherichia coli Strain of the Gastroenteritis Microbiome
Seung-Hak Cho, Soon Young Han, Yeon-Ho Kang
Osong Public Health and Research Perspectives.2014; 5(3): 156. CrossRef
A survey of the frequency of aminoglycoside antibiotic-resistant genotypes and phenotypes inEscherichia coliin broilers with septicaemia in Hebei, China
F.Y. Zhang, S.Y. Huo, Y.R. Li, R. Xie, X.J. Wu, L.G. Chen, Y.H. Gao
British Poultry Science.2014; 55(3): 305. CrossRef
Prevalence of Antimicrobial Resistance in Escherichia coli Strains Isolated from Fishery Workers
Hyun-Ho Shin, Seung-Hak Cho
Osong Public Health and Research Perspectives.2013; 4(2): 72. CrossRef

First
Prev
Page of 1
Next
Last

Search