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Brief Report
- Isolation and identification of monkeypox virus MPXV-ROK-P1-2022 from the first case in the Republic of Korea
- Jin-Won Kim, Minji Lee, Hwachul Shin, Chi-Hwan Choi, Myung-Min Choi, Jee Woong Kim, Hwajung Yi, Cheon-Kwon Yoo, Gi-Eun Rhie
- Osong Public Health Res Perspect. 2022;13(4):308-311. Published online August 31, 2022
- DOI: https://doi.org/10.24171/j.phrp.2022.0232
- 7,798 View
- 172 Download
- 9 Web of Science
- 9 Crossref
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Graphical Abstract
Abstract
PDF 
- Objectives
Monkeypox outbreaks in nonendemic countries have been reported since early May 2022. The first case of monkeypox in the Republic of Korea was confirmed in a patient who traveled to Europe in June 2022, and an attempt was made to isolate and identify the monkeypox virus (MPXV) from the patient’s specimens.
Methods
Clinical specimens from the patient were inoculated in Vero E6 cells. The isolated virus was identified as MPXV by the observation of cytopathic effects on Vero E6 cells, transmission electron microscopy, conventional polymerase chain reaction (PCR), and sequencing of PCR products.
Results
Cytopathic effects were observed in Vero E6 cells that were inoculated with skin lesion swab eluates. After multiple passages from the primary culture, orthopoxvirus morphology was observed using transmission electron microscopy. In addition, both MPXV-specific (F3L and ATI) and orthopoxvirus-specific genes (A39R, B2R, and HA) were confirmed by conventional PCR and Sanger sequencing.
Conclusion
These results indicate the successful isolation and identification of MPXV from the first patient in the Republic of Korea. The isolated virus was named MPXV-ROK-P1-2022. -
Citations
Citations to this article as recorded by
- Ultrasensitive one-pot detection of monkeypox virus with RPA and CRISPR in a sucrose-aided multiphase aqueous system
Yue Wang, Yixin Tang, Yukang Chen, Guangxi Yu, Xue Zhang, Lihong Yang, Chenjie Zhao, Pei Wang, Song Gao, Frederick S. B. Kibenge, Ruijie Deng, Wei Chen, Shuang Yang
Microbiology Spectrum.2024;[Epub] CrossRef - Molecular Methods for Diagnosis of Monkeypox: A Mini-review
Rodrigo Michelini de Oliveira Thomasi, Thais da Silva Correa, Dalise Silva do Carmo, Déborah Fernandes Rodrigues, Luiz Vinicius da Silva Correa, Sandra Rodrigues Xavier, Líria Souza Silva, Jonatas Oliveira da Silva, Michelli dos Santos, Alessandra da Silv
Current Molecular Medicine.2024; 24(10): 1208. CrossRef - Epidemiological, Clinical, and Virological Investigation of the First Four Cases of Monkeypox in Cartagena during the 2022 Outbreak
Steev Loyola, Mashiel Fernández-Ruiz, Doris Gómez-Camargo
Pathogens.2023; 12(2): 159. CrossRef - 원숭이두창바이러스의 분리 배양과 전장유전체 정보 분석
민지 이, 진원 김, 치환 최, 화철 신, 명민 최, 상은 이, 화중 이, 윤석 정
Public Health Weekly Report.2023; 16(15): 464. CrossRef - Overview of Diagnostic Methods, Disease Prevalence and Transmission of Mpox (Formerly Monkeypox) in Humans and Animal Reservoirs
Ravendra P. Chauhan, Ronen Fogel, Janice Limson
Microorganisms.2023; 11(5): 1186. CrossRef - How to cope with suspected mpox patients in the outpatient clinic
Nam Joong Kim, Sun Huh
Journal of the Korean Medical Association.2023; 66(5): 325. CrossRef - An Updated Review on Monkeypox Viral Disease: Emphasis on Genomic Diversity
Ali Rabaan, Nada Alasiri, Mohammed Aljeldah, Abeer Alshukairiis, Zainab AlMusa, Wadha Alfouzan, Abdulmonem Abuzaid, Aref Alamri, Hani Al-Afghani, Nadira Al-baghli, Nawal Alqahtani, Nadia Al-baghli, Mashahed Almoutawa, Maha Mahmoud Alawi, Mohammed Alabdull
Biomedicines.2023; 11(7): 1832. CrossRef - Monkeypox (Mpox) virus isolation and ultrastructural characterisation from a Brazilian human sample case
Milene Dias Miranda, Gabriela Cardoso Caldas, Vivian Neuza Ferreira, Ortrud Monika Barth, Aline de Paula Dias da Silva, Mayara Secco Torres Silva, Beatriz Grinsztejn, Valdiléa Gonçalves Veloso, Thiago Moreno Souza, Edson Elias da Silva, Debora Ferreira Ba
Memórias do Instituto Oswaldo Cruz.2023;[Epub] CrossRef - Isolation and Characterization of Monkeypox Virus from the First Case of Monkeypox — Chongqing Municipality, China, 2022
Baoying Huang, Hua Zhao, Jingdong Song, Li Zhao, Yao Deng, Wen Wang, Roujian Lu, Wenling Wang, Jiao Ren, Fei Ye, Houwen Tian, Guizhen Wu, Hua Ling, Wenjie Tan
China CDC Weekly.2022; 4(46): 1019. CrossRef
- Ultrasensitive one-pot detection of monkeypox virus with RPA and CRISPR in a sucrose-aided multiphase aqueous system
Original Article
- Gene Expression and Identification Related to Fluconazole Resistance of
Candida glabrata Strains - Jae Il Yoo, Chi Won Choi, Kyeong Min Lee, Yeong Seon Lee
- Osong Public Health Res Perspect. 2010;1(1):36-41. Published online December 31, 2010
- DOI: https://doi.org/10.1016/j.phrp.2010.12.009
- 3,271 View
- 19 Download
- 10 Crossref
-
Abstract
PDF
- Objectives
Candida glabrata has become one of the most common causes of Candida bloodstream infections worldwide. Some strains of C. glabrata may be intermediately resistant to all azoles. The several possible mechanisms of azole resistance have been reported previously, but the exact resistant mechanism is not clear. In this study, we identified differentially expressed genes (DEGs) of fluconazole-resistant C. glabrata and compared the gene expression of fluconazole-resistant strains with that of fluconazole-susceptible strains to identify gene corresponding to fluconazole resistance.
Methods
Using antifungal susceptibility test, several C. glabrata strains were selected and used for further study. The expression of CgCDR1 and CgCDR2 genes was investigated by slot hybridization against fluconazole-susceptible, -resistant, and resistant-induced strains. In addition, ERG3 and ERG11 genes were sequenced to analyze DNA base substitution. DEGs were identified by reverse transcription-polymerase chain reaction using DEG kit composed of 120 random primers.
Results
In slot hybridization, CgCDR1 gene was expressed more than CgCDR2 gene in resistant strains. Though base substitution of ERG11 and ERG3 genes was observed in several base sequences, just one amino acid change was identified in resistant strain. In the results of reverse transcription-polymerase chain reaction, 44 genes were upregulated and 34 genes were downregulated. Among them, adenosine triphosphate-binding cassette transporter-related genes, fatty acid desaturase, lyase, and hypothetical protein genes were upregulated and aldehyde dehydrogenase, oxidoreductase, and prohibitin-like protein genes were downregulated. Other DEGs were also identified.
Conclusion
This study showed that CgCDR1 gene was more closely related to fluconazole resistance of C. glabrata than CgCDR2 gene. In addition, several other genes related with fluconazole resistance of C. glabrata were identified. -
Citations
Citations to this article as recorded by- Two promising Bacillus-derived antifungal lipopeptide leads AF4 and AF5 and their combined effect with fluconazole on the in vitro Candida glabrata biofilms
Madduri Madhuri, Shivaprakash M. Rudramurthy, Utpal Roy
Frontiers in Pharmacology.2024;[Epub] CrossRef - Molecular Mechanisms Associated with Antifungal Resistance in Pathogenic Candida Species
Karolina M. Czajka, Krishnan Venkataraman, Danielle Brabant-Kirwan, Stacey A. Santi, Chris Verschoor, Vasu D. Appanna, Ravi Singh, Deborah P. Saunders, Sujeenthar Tharmalingam
Cells.2023; 12(22): 2655. CrossRef - Candida glabrata: Pathogenicity and Resistance Mechanisms for Adaptation and Survival
Yahaya Hassan, Shu Yih Chew, Leslie Thian Lung Than
Journal of Fungi.2021; 7(8): 667. CrossRef - Candidiasis and Mechanisms of Antifungal Resistance
Somanon Bhattacharya, Sutthichai Sae-Tia, Bettina C. Fries
Antibiotics.2020; 9(6): 312. CrossRef -
A Transcriptomics Approach To Unveiling the Mechanisms of
In Vitro
Evolution towards Fluconazole Resistance of a
Candida glabrata
Clinical Isolate
Mafalda Cavalheiro, Catarina Costa, Ana Silva-Dias, Isabel M. Miranda, Can Wang, Pedro Pais, Sandra N. Pinto, Dalila Mil-Homens, Michiyo Sato-Okamoto, Azusa Takahashi-Nakaguchi, Raquel M. Silva, Nuno P. Mira, Arsénio M. Fialho, Hiroji Chibana, Acácio G. R
Antimicrobial Agents and Chemotherapy.2019;[Epub] CrossRef - Clonal Spread of Candida glabrata Bloodstream Isolates and Fluconazole Resistance Affected by Prolonged Exposure: a 12-Year Single-Center Study in Belgium
Berdieke Goemaere, Katrien Lagrou, Isabel Spriet, Marijke Hendrickx, Pierre Becker
Antimicrobial Agents and Chemotherapy.2018;[Epub] CrossRef - Candida antifungal drug resistance in sub-Saharan African populations: A systematic review
Charlene Wilma Joyce Africa, Pedro Miguel dos Santos Abrantes
F1000Research.2017; 5: 2832. CrossRef - Expression Patterns of ABC Transporter Genes in Fluconazole-Resistant Candida glabrata
Atefeh Abdollahi Gohar, Hamid Badali, Tahereh Shokohi, Mojtaba Nabili, Nasrin Amirrajab, Maryam Moazeni
Mycopathologia.2017; 182(3-4): 273. CrossRef - Glabridin induces overexpression of two major apoptotic genes, MCA1 and NUC1 , in Candida albicans
Mojtaba Nabili, Maryam Moazeni, Mohammad Taghi Hedayati, Parisa Aryamlo, Atefeh Abdollahi Gohar, Seyed Mehdi Madani, Hamed Fathi
Journal of Global Antimicrobial Resistance.2017; 11: 52. CrossRef - Candida antifungal drug resistance in sub-Saharan African populations: A systematic review
Charlene Wilma Joyce Africa, Pedro Miguel dos Santos Abrantes
F1000Research.2016; 5: 2832. CrossRef
- Two promising Bacillus-derived antifungal lipopeptide leads AF4 and AF5 and their combined effect with fluconazole on the in vitro Candida glabrata biofilms
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