Osong Public Health and Research Perspectives

Original Articles

Prevalence, multidrug resistance, and biofilm formation of Vibrio parahaemolyticus isolated from fish mariculture environments in Cat Ba Island, Vietnam: Kim Cuc Thi Nguyen, Phuc Hung Truong, Hoa Truong Thi, Xuan Tuy Ho, Phu Van Nguyen; Osong Public Health Res Perspect. 2024;15(1):56-67. Published online February 19, 2024; DOI: https://doi.org/10.24171/j.phrp.2023.0181

3,003 View
121 Download
2 Web of Science
5 Crossref

Graphical Abstract Abstract PDF Supplementary Material

Objectives
Vibrio parahaemolyticus is a major foodborne pathogen in aquatic animals and a threat to human health worldwide. This study investigated the prevalence, antimicrobial resistance, antimicrobial resistance genes (ARGs), and biofilm formation of V. parahaemolyticus strains isolated from fish mariculture environments in Cat Ba Island, Vietnam. Methods: In total, 150 rearing water samples were collected from 10 fish mariculture farms in winter and summer. A polymerase chain reaction assay was used to identify V. parahaemolyticus, its virulence factors, and ARGs. The antimicrobial resistance patterns and biofilm formation ability of V. parahaemolyticus strains were investigated using the disk diffusion test and a microtiter plate-based crystal violet method, respectively. Results: Thirty-seven V. parahaemolyticus isolates were recovered from 150 samples. The frequencies of the tdh and trh genes among V. parahaemolyticus isolates were 8.1% and 21.6%, respectively. More than 90% of isolates were susceptible to ceftazidime, cefotaxime, and chloramphenicol, but over 72% were resistant to ampicillin, tetracycline, and erythromycin. Furthermore, 67.57% of isolates exhibited multidrug resistance. The presence of ARGs related to gentamicin (aac(3)-IV), tetracycline (tetA) and ciprofloxacin (qnrA) in V. parahaemolyticus isolates was identified. Conversely, no ARGs related to ampicillin or erythromycin resistance were detected. Biofilm formation capacity was detected in significantly more multidrug-resistant isolates (64.9%) than non-multidrug-resistant isolates (18.9%). Conclusion: Mariculture environments are a potential source of antibiotic-resistant V. parahaemolyticus and a hotspot for virulence genes and ARGs diffusing to aquatic environments. Thus, the prevention of antibiotic-resistant foodborne vibriosis in aquatic animals and humans requires continuous monitoring.

Citations

Citations to this article as recorded by

Exploring the antibiofilm efficacy of pyrimidines against Vibrio parahaemolyticus, mechanisms and their potential food processing applications
Olajide Sunday Faleye, Jin-Hyung Lee, Jintae Lee
LWT.2025; 215: 117229. CrossRef
The role of sea fish meat in the transmission of Vibrio parahaemolyticus to humans: An in-depth analysis of seasonal and species-specific variations
Maged A. Al-Garadi, Dhary Alewy Almashhadany, Rasha N. Aziz, Dheyazan M. Ali Al-Qabili, Ohoud S. Alhumaidan, Hanouf Alnuwaysir, Al-Hammadi Mohammed Ali, Essam Sayed, A. M. Alabsi
Veterinary World.2025; : 348. CrossRef
Multidrug Resistance, Biofilm-Forming Ability, and Molecular Characterization of Vibrio Species Isolated from Foods in Thailand
Watcharapong Mitsuwan, Ratchadaporn Boripun, Phirabhat Saengsawang, Sutsiree Intongead, Sumaree Boonplu, Rawiwan Chanpakdee, Yukio Morita, Sumalee Boonmar, Napapat Rojanakun, Natnicha Suksriroj, Chollathip Ruekaewma, Titima Tenitsara
Antibiotics.2025; 14(3): 235. CrossRef
A New Strategy: Antibiotic Circumstances for Rational Drug use Against Clinical Aeromonas hydrophila And Bacterial Properties of This Bacteria on Different Agars
Nurdan Filik, Fethi Filik, Ayşegül Kubilay
Acta Aquatica Turcica.2024;[Epub] CrossRef
Passive immunoprophylaxis with Ccombodies against Vibrio parahaemolyticus in Pacific white shrimp (Penaeus vannamei)
Wanna Sirimanapong, Prawporn Thaijongrak, Chiranan Sudpraseart, Dennis Berbulla Bela-ong, Abigail Joy D. Rodelas-Angelia, Mark Rickard N. Angelia, Seungbeom Hong, Jaesung Kim, Kim D. Thompson, Tae Sung Jung
Fish & Shellfish Immunology.2024; 154: 109973. CrossRef

Prevalence of Antimicrobial Resistance in Escherichia coli Strains Isolated from Fishery Workers: Hyun-Ho Shin, Seung-Hak Cho; Osong Public Health Res Perspect. 2013;4(2):72-75. Published online April 30, 2013; DOI: https://doi.org/10.1016/j.phrp.2013.03.001

3,910 View
22 Download
6 Crossref

Abstract PDF

Objectives
This study aimed to characterize the prevalence of antibiotic resistance in Escherichia coli isolates from the fecal samples of fishery workers who work in fish farms and often use antibiotics for the feeding fishes.
Methods
Seventy-three E. coli strains isolated from the fecal samples of fishery workers and 180 isolates from a control group of restaurant workers were tested for antibiotic resistance by agar disk diffusion with 16 antimicrobial agents.
Results
About 30% of isolates from each group showed antimicrobial resistance to ampicillin, and 60% of isolates from fishery workers and 41% from restaurant workers were resistant to tetracycline. The isolates showed higher resistance to cephalothin and cefoxitin than to other cephem antibiotics and to gentamicin than to other aminogycosides. Our data indicated that fecal E. coli isolates from fishery workers showed higher antibiotic resistance than those of non-fishery workers (restaurant workers), especially to cephalothin, tetracycline, and trimethoprim–sulfamethoxazole (p < 0.05). However, rates of multidrug resistance were similar among the fishery workers and restaurant workers.
Conclusion
Frequent use of antibiotics may cause increased antibiotic resistance in the human microbiome.

Citations

Citations to this article as recorded by

Enhanced catalytic performance of Cu2ZnSnS4/MoS2 nanocomposites based counter electrode for Pt-free dye-sensitized solar cells
P. Baskaran, K.D. Nisha, S. Harish, H. Ikeda, J. Archana, M. Navaneethan
Journal of Alloys and Compounds.2022; 894: 162166. CrossRef
Influence of Na2S treatment on CZTS/Mo interface in Cu2ZnSnS4 solar cells annealed in sulfur-free atmosphere
Jie Guo, Shuaihui Sun, Bin Liu, Ruiting Hao, Licun Sun
Optik.2021; 242: 166998. CrossRef
Evaluating antimicrobial resistance in the global shrimp industry
Kelly Thornber, David Verner‐Jeffreys, Steve Hinchliffe, Muhammad Meezanur Rahman, David Bass, Charles R. Tyler
Reviews in Aquaculture.2020; 12(2): 966. CrossRef
Antibiotic-resistant pathogens in the occupational environment
Anna Ławniczek-Wałczyk, Rafał L. Górny
Occupational Safety – Science and Practice.2019; 579(12): 9. CrossRef
Human, animal and environmental contributors to antibiotic resistance in low-resource settings: integrating behavioural, epidemiological and One Health approaches
Emily K. Rousham, Leanne Unicomb, Mohammad Aminul Islam
Proceedings of the Royal Society B: Biological Sci.2018; 285(1876): 20180332. CrossRef
Possibility of CTX-M-14 Gene Transfer from Shigella sonnei to a Commensal Escherichia coli Strain of the Gastroenteritis Microbiome
Seung-Hak Cho, Soon Young Han, Yeon-Ho Kang
Osong Public Health and Research Perspectives.2014; 5(3): 156. CrossRef

First
Prev
Page of 1
Next
Last

Search