Osong Public Health and Research Perspectives

Original Articles

Prevalence of plasmid-mediated AmpC β-lactamases among uropathogenic Escherichia coli isolates in southwestern Iran: Nabi Jomehzadeh, Khadijeh Ahmadi, Zahra Rahmani; Osong Public Health Res Perspect. 2021;12(6):390-395. Published online December 1, 2021; DOI: https://doi.org/10.24171/j.phrp.2021.0272

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Objectives
This study was undertaken to evaluate AmpC β-lactamase-producing Escherichia coli urine isolates and to characterize the frequency of plasmid-mediated AmpC (pAmpC)-encoding genes.
Methods
Antimicrobial susceptibility tests were performed using the disk diffusion technique. AmpC β-lactamase production was assessed with a phenotypic inhibitor-based method. The presence of 6 pAmpC-encoding cluster genes was detected by multiplex polymerase chain reaction (PCR).
Results
The proportion of antibiotic resistance of E. coli isolates ranged from 7.4% to 90.5%, and more than half (51.6%) of the total isolates were multidrug-resistant (MDR). Among the 95 E. coli isolates, 60 (63.2%) were found to be cefoxitin-resistant, but only 14 (14.7%) isolates were confirmed as AmpC β-lactamase-producers. In the PCR assay, pAmpC-encoding genes were found in 15 (15.8%) isolates, and blaDHA was the most prevalent type. However, blaFOX, blaMOX, and blaACC genes were not detected in the isolates.
Conclusion
Our findings contributed valuable information concerning antibiotic resistance, confirmatory phenotypic testing for AmpC production, and pAmpC β-lactamase gene content in E. coli isolates in southwestern Iran. The level of MDR recorded in AmpC-producing strains of this study was worrying; therefore, implementing strong infection control approaches to reduce the MDR burden is recommended.

Citations

Citations to this article as recorded by

A review of the mechanisms that confer antibiotic resistance in pathotypes of E. coli
Sina Nasrollahian, Jay P. Graham, Mehrdad Halaji
Frontiers in Cellular and Infection Microbiology.2024;[Epub] CrossRef
Association Between Uropathogenic Escherichia coli Virulence Genes and Severity of Infection and Resistance to Antibiotics
Sofía Alejandra Fonseca-Martínez, Ruth Aralí Martínez-Vega, Ana Elvira Farfán-García, Clara Isabel González Rugeles, Libeth Yajaira Criado-Guerrero
Infection and Drug Resistance.2023; Volume 16: 3707. CrossRef
Extended-spectrum β-lactamases producing Enterobacteriaceae (ESBL-PE) prevalence in Nepal: A systematic review and meta-analysis
Christina Khadka, Manita Shyaula, Gopiram Syangtan, Shrijana Bista, Reshma Tuladhar, Anjana Singh, Dev Raj Joshi, Lok R. Pokhrel, Prabin Dawadi
Science of The Total Environment.2023; 901: 166164. CrossRef
Prevalence of Extended-Spectrum β-Lactamase-Resistant Genes in Escherichia coli Isolates from Central China during 2016–2019
Zui Wang, Qin Lu, Xiaohui Mao, Li Li, Junfeng Dou, Qigai He, Huabin Shao, Qingping Luo
Animals.2022; 12(22): 3191. CrossRef
Molecular detection and characterization of Shigella spp. harboring extended-spectrum β-lactamase genes in children with diarrhea in northwest Iran
Sahar Sabour, Amir Teimourpour, Jafar Mohammadshahi, Hadi Peeridogaheh, Roghayeh Teimourpour, Taher Azimi, Zahra Hosseinali
Molecular and Cellular Pediatrics.2022;[Epub] CrossRef
Plasmid-mediated AmpC β-Lactamase Gene Analysis in Klebsiella Pneumoniae Clinical Isolates
Nabi Jomehzadeh, Khadijeh Ahmadi, Hasti Shaabaninejad, Gholamali Eslami
Biomedical and Biotechnology Research Journal (BBR.2022; 6(4): 582. CrossRef

High Prevalence of AmpC β-Lactamases in Clinical Isolates of Escherichia coli in Ilam, Iran: Abbas Maleki, Afra Khosravi, Sobhan Ghafourian, Iraj Pakzad, Shiva Hosseini, Rashid Ramazanzadeh, Nourkhoda Sadeghifard; Osong Public Health Res Perspect. 2015;6(3):201-204. Published online June 30, 2015; DOI: https://doi.org/10.1016/j.phrp.2015.02.001

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Abstract PDF

Objectives
Widespread use of β-lactam antibiotics could cause resistance to this group of antibiotics in pathogenic bacteria through the production of the enzyme β-lactamases. The aim of this study is to determine the molecular detection of AmpC β-lactamases among clinical Escherichia coli isolated from Ilam hospitals in Ilam, Iran.
Methods
One hundred and twelve clinical isolates of E. coli were collected from hospitalized patients and were identified by biochemical tests. They were evaluated for extended spectrum beta-lactamases (ESBLs) production, and the positive strains were subjected to AmpC enzymes; for detection of AmpC cluster genes, multiplex polymerase chain reaction was applied.
Results
The analysis showed 62.5% of isolates were ESBLs positive and that five strains revealed the AmpC cluster genes. This is the first report of FOXM cluster genes in E. coli in Iran.
Conclusion
Based on our results, the prevalence of AmpC β-lactamases is increasing in Iran, which caused failure in antibiotic therapy. So, the current study recommended the revision of antibiotic policy in Iranian hospitals.

Citations

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Prevalence of ESBL and AmpC genes in E. coli isolates from urinary tract infections in the north of Iran
M. Sadeghi, H. Sedigh Ebrahim-Saraie, A. Mojtahedi
New Microbes and New Infections.2022; 45: 100947. CrossRef
Amp-CKlebsiella-Induced Chorioamnionitis and Associated Abnormalities in Cardiotocography: A Case Report with a Narrative Review
Sufia Athar, Anvar P. Vellamgot, Lolwa Mohammed Alansari, Mohd Adnan
Case Reports in Infectious Diseases.2022; 2022: 1. CrossRef
Molecular detection of plasmid-derived AmpC β-lactamase among clinical strains of Enterobacteriaceae in Bahrain
Ronni Mol Joji, Ali Ebrahim Al-Mahameed, Thamer Al Jishi, Dania Ismail Fatani, Nermin K. Saeed, Ahmed Jaradat, Hicham Ezzat, Khalid Mubarak Bindayna
Annals of Thoracic Medicine.2021; 16(3): 287. CrossRef
Molecular characteristics, antimicrobial resistance profiles, and antibiotic resistance determinants in uropathogenic fluoroquinolone resistant-Escherichia coli isolates
Hassan Valadbeigi, Masoumeh HatamiLak, Abbas Maleki, Ebrahim Kouhsari, Nourkhoda Sadeghifard
Gene Reports.2020; 18: 100584. CrossRef
High prevalence of blaCMY AmpC beta-lactamase in ESBL co-producing Escherichia coli and Klebsiella spp. clinical isolates in the northeast of Iran
Kobra Salimiyan Rizi, Arman Mosavat, Masoud Youssefi, Saeid Amel Jamehdar, Kiarash Ghazvini, Hadi Safdari, Yousef Amini, Hadi Farsiani
Journal of Global Antimicrobial Resistance.2020; 22: 477. CrossRef
Evaluating the antimicrobial resistance and frequency of AmpC β-lactamases bla gene in Gram-negative bacteria isolates collected from selected hospitals of Iran: A multicenter retrospective study
Fatemeh Fallah, Taher Azimi, Leila Azimi, Abdollah Karimi, Mohammad Rahbar, Mehdi Shirdoust, Iraj Sedighi, Ata Saadat Sadeghi, Shahnaz Armin
Gene Reports.2020; 21: 100868. CrossRef
Plasmid-Mediated AmpC β-Lactamase CITM and DHAM Genes Among Gram-Negative Clinical Isolates

Subhas Chandra Aryal, Milan Kumar Upreti, Anil Kumar Sah, Mehraj Ansari, Krishus Nepal, Binod Dhungel, Nabaraj Adhikari, Binod Lekhak, Komal Raj Rijal
Infection and Drug Resistance.2020; Volume 13: 4249. CrossRef
Isolation and molecular characterization of multidrug-resistant Escherichia coli from chicken meat
Md. Masudur Rahman, Asmaul Husna, Hatem A. Elshabrawy, Jahangir Alam, Nurjahan Yasmin Runa, A. T. M. Badruzzaman, Nahid Arjuman Banu, Mohammad Al Mamun, Bashudeb Paul, Shobhan Das, Md. Mahfujur Rahman, A. T. M. Mahbub-E-Elahi, Ahmed S. Khairalla, Hossam M
Scientific Reports.2020;[Epub] CrossRef
PHENOTYPIC DETECTION OF AMPC β-LACTAMASE ENZYME IN GRAM-NEGATIVE BACILLI
Khanda Anoar, Sherko Omer, Bayan Majid, Hero Rahim, Shno Muhammed
JOURNAL OF SULAIMANI MEDICAL COLLEGE.2018; 8(2): 57. CrossRef
Bacteraemia due to AmpC β-lactamase-producing Escherichia coli in hospitalized cancer patients: risk factors, antibiotic therapy, and outcomes
Qing Zhang, Wenfang Zhang, Zheng Li, Changsen Bai, Ding Li, Shan Zheng, Peng Zhang, Sihe Zhang
Diagnostic Microbiology and Infectious Disease.2017; 88(3): 247. CrossRef

Characterization of Plasmid-Mediated AmpC and Carbapenemases among Iranain Nosocomial Isolates of Klebsiella pneumoniae Using Phenotyping and Genotyping Methods: Alireza Japoni-Nejad, Ehsanollah Ghaznavi-Rad, Alex van Belkum; Osong Public Health Res Perspect. 2014;5(6):333-338. Published online December 31, 2014; DOI: https://doi.org/10.1016/j.phrp.2014.09.003

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31 Download
14 Crossref

Abstract PDF

Objectives
Plasmid-mediated AmpC β-lactamases (PMABLs) and carbapenemases are emerging groups of antimicrobial-resistance determinants. The aims of the study were to evaluate the occurrence of PMABLs and carbapenemases in clinical isolates of Klebsiella pneumoniae and compare the test performance of various phenotypic methods for detection of these enzymes in Iran.
Methods
A total of 100 K. pneumoniae isolates were collected from clinical specimens obtained in Valiasr Hospital. AmpC production in all isolates was determined using the AmpC disk test, the cephamycin Hodge test, the AmpC Etest, and the boronic acid combined-disk test. In addition, carbapenemase production was determined using the modified Hodge test, the EDTA disk synergy test, and the boronic acid combined-disk test. The performances of various phenotypic methods were evaluated by the comparison of their results with polymerase chain reaction (PCR) method as the gold standard.
Results
Of the 100 isolates, 19 (19%) were demonstrated to harbor the PMABL-resistance gene by the multiplex PCR method. The PCR result indicated the presence of carbapenemase genes in 12 isolates. The performance of various phenotypic tests carried out for detection of carbapenemase-producing isolates varied widely, ranging in sensitivity from 30% to 100% and in specificity from 90.8% to 100%.
Conclusion
This is the first report of MOX-type AmpC β-lactamase and bla_GES in K. pneumoniae in Iran. A comparison of the phenotypic methods showed that a combination of cefoxitin plus boronic acid is optimal for detecting plasmid-mediated AmpC enzymes in K. pneumoniae, whereas the implementation of molecular methods is often complex, requires specially trained personnel, and is associated with higher costs.

Citations

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The Global and Regional Prevalence of Hospital-Acquired Carbapenem-Resistant Klebsiella pneumoniae Infection: A Systematic Review and Meta-analysis
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High frequency of NDM-1 and OXA-48 carbapenemase genes among Klebsiella pneumoniae isolates in central Iran
Elnaz Abbasi, Ehsanollah Ghaznavi-Rad
BMC Microbiology.2023;[Epub] CrossRef
Detection of ESBL and AmpC producing Klebsiella pneumoniae ST11 and ST147 from urinary tract infections in Iran
Shaghayegh Shahkolahi, Pegah Shakibnia, Shahla Shahbazi, Samira Sabzi, Farzad Badmasti, Mohammad Reza Asadi Karam, Mehri Habibi
Acta Microbiologica et Immunologica Hungarica.2022;[Epub] CrossRef
Plasmid-mediated AmpC β-Lactamase Gene Analysis in Klebsiella Pneumoniae Clinical Isolates
Nabi Jomehzadeh, Khadijeh Ahmadi, Hasti Shaabaninejad, Gholamali Eslami
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Infectious Disorders - Drug Targets .2021; 21(1): 119. CrossRef
An investigation of extended-spectrum β-lactamases (ESBLs) in Klebsiella isolated from foodborne outbreaks in Iran
Farnaz Hajikarim, Mohammad Mehdi Soltan Dallal, Mohammad Reza Pourmand, Milad Abdi
Gene Reports.2020; 19: 100632. CrossRef
Prevalence and Mechanisms of Carbapenem Resistance in Klebsiella pneumoniae and Escherichia coli: A Systematic Review and Meta-Analysis of Cross-Sectional Studies from Iran
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Shaymaa H. Abdel-Rhman, Grzegorz Woźniakowski
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Multiplex PCR to detect pAmpC β-lactamases among enterobacteriaceae at a tertiary care laboratory in Mumbai, India
Mubin Kazi, Kanchan Ajbani, Jeffrey A. Tornheim, Anjali Shetty, Camilla Rodrigues
Microbiology.2019; 165(2): 246. CrossRef
AmpC β lactamases in Urinary Klebsiella pneumoniae Isolates: First Report of ACC Type AmpC β-lactamase Resistance in Iran
Maryam Ghane, Laleh Babaeekhou, Mahdi Jafar Shanjani
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