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- Impact of Time Delay in Processing Blood Sample on Next Generation Sequencing for Transcriptome Analysis
- Jae-Eun Lee, So-Young Jung, So-Youn Shin, Young-Youl Kim
- Osong Public Health Res Perspect. 2018;9(3):130-132. Published online June 30, 2018
- DOI: https://doi.org/10.24171/j.phrp.2018.9.3.09
- 4,812 View
- 37 Download
- 1 Crossref
Original Article
- Instability at Short Tandem Repeats in Lymphoblastoid Cell Lines
- Jae-Eun Lee, Eun-Jung Hong, Ji-Hyun Kim, So Youn Shin, Young-Youl Kim, Bok-Ghee Han
- Osong Public Health Res Perspect. 2013;4(4):194-196. Published online August 31, 2013
- DOI: https://doi.org/10.1016/j.phrp.2013.06.003
- 3,314 View
- 15 Download
- 1 Crossref
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Abstract
PDF - Objectives
Epstein Barr virus (EBV)-transformed lymphoblastoid cell lines (LCLs) are a useful biological resource, however, genomic variations can happen during the generation and immortalization processes of LCLs. The purpose of this study was to identify genomic variations in LCL DNA compared with matched blood DNA using short tandem repeats (STRs) analysis.
Methods
We analyzed 15 STRs with blood DNA and their matched LCL DNA samples from 6645 unrelated healthy individuals.
Results
Mutations (such as repeat variations and triallelic patterns) of 15 STR loci were detected in 612 LCL DNAs (9.2% of total) without mutations in their matched blood DNA. The repeat variations of 15 STRs were detected in 526 LCL DNAs (mutation rate = 0.0792) and triallelic patterns were identified in 123 (mutation rate = 0.0185). Among 15 STRs, the most common repeat variations (n = 214, mutation rate = 0.0322) and triallelic patterns (n = 17, mutation rate = 0.0026) were found at FGA locus.
Conclusion
Our study shows that mutations in STRs can occur during generation and immortalization of LCLs. -
Citations
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Christopher Korch, Erin M. Hall, Wilhelm G. Dirks, Margaret Ewing, Mark Faries, Marileila Varella‐Garcia, Steven Robinson, Douglas Storts, Jacqueline A. Turner, Ying Wang, Edward C. Burnett, Lyn Healy, Douglas Kniss, Richard M. Neve, Raymond W. Nims, Yvon
International Journal of Cancer.2018; 142(3): 561. CrossRef
- Authentication of M14 melanoma cell line proves misidentification of MDA‐MB‐435 breast cancer cell line
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