Objectives
Coxsackievirus A group 16 strain (CVA16) is one of the predominant causative agents of hand, foot, and mouth disease (HFMD). Methods
Using a specimen from a male patient with HFMD, we isolated and performed sequencing of the Korean CVA16 strain and compared it with a G10 reference strain. Also, we were investigated the effects of medicinal plant extract on the cytopathic effects (CPE) by CPE reduction assay against Korean CVA16. Results
Phylogenetic analysis showed that the Korean CVA16 isolate belonged to cluster B-1 and was closely related to the strain PM-15765-00 isolated in Malaysia in 2000. The Korean CVA16 isolate showed 73.2% nucleotide identity to the G10 prototype strain and 98.7% nucleotide identity to PM-15765-00. Next, we assessed whether the Korean CVA16 isolate could be used for in vitro screening of antiviral agents to treat HFMD infection. Vero cells infected with the Korean CVA16 isolate showed a cytopathic effect 2 days after the infection, and the treatment of cells with Cornus officinalis, Acer triflorum, Pulsatilla koreana, and Clematis heracleifolia var. davidiana Hemsl extracts exhibited strong antiviral activity against CVA16. Conclusion
Collectively, our work provides potential candidates for the development of vaccine and novel drugs to treat the CVA16 strain isolated from a Korean patient.
Citations
Citations to this article as recorded by
Recent Advances in Anti-Coxsackievirus A16 Viral Drug Research Xiaolan Yuan, Ziwei Liu, Li Wan, Wei Liu, Yan Huang, Shuang Cao Future Medicinal Chemistry.2023; 15(1): 97. CrossRef
A Review with Updated Perspectives on the Antiviral Potentials of Traditional Medicinal Plants and Their Prospects in Antiviral Therapy Nur Fadlin Saifulazmi, Emelda Rosseleena Rohani, Sarahani Harun, Hamidun Bunawan, Hamizah Shahirah Hamezah, Nor Azlan Nor Muhammad, Kamalrul Azlan Azizan, Qamar Uddin Ahmed, Sharida Fakurazi, Ahmed Mediani, Murni Nazira Sarian Life.2022; 12(8): 1287. CrossRef
Medicinal plants: Treasure for antiviral drug discovery Sofi Imtiyaz Ali, Wajid Mohammad Sheikh, Muzafar Ahmad Rather, Venugopalan Venkatesalu, Showkeen Muzamil Bashir, Showkat Ul Nabi Phytotherapy Research.2021; 35(7): 3447. CrossRef
Seasonal Distribution and Meteorological Factors Associated with Hand, Foot, and Mouth Disease among Children in Xi’an, Northwestern China Tianci Guo, Jifeng Liu, Junjiang Chen, Yao Bai, Yong Long, Baozhong Chen, Shuxuan Song, Zhongjun Shao, Kun Liu The American Journal of Tropical Medicine and Hygi.2020; 102(6): 1253. CrossRef
Short-term effects of meteorological factors and air pollution on childhood hand-foot-mouth disease in Guilin, China Guoqi Yu, Yonghong Li, Jiansheng Cai, Dongmei Yu, Jiexia Tang, Wenwen Zhai, Yi Wei, Shiyi Chen, Quanhui Chen, Jian Qin Science of The Total Environment.2019; 646: 460. CrossRef
The effect of temperature on childhood hand, foot and mouth disease in Guangdong Province, China, 2010–2013: a multicity study Zece Xu, Wenqi Hu, Kedi Jiao, Ci Ren, Baofa Jiang, Wei Ma BMC Infectious Diseases.2019;[Epub] CrossRef
Surveillance, epidemiology, and pathogen spectrum of hand, foot, and mouth disease in mainland of China from 2008 to 2017 Tianjiao Ji, Taoli Han, Xiaojuan Tan, Shuangli Zhu, Dongmei Yan, Qian Yang, Yang Song, Aili Cui, Yan Zhang, Naiying Mao, Songtao Xu, Zhen Zhu, Dandan Niu, Yong Zhang, Wenbo Xu Biosafety and Health.2019; 1(1): 32. CrossRef
A potential therapeutic neutralization monoclonal antibody specifically against multi-coxsackievirus A16 strains challenge Ruixiao Du, Qunying Mao, Yalin Hu, Shuhui Lang, Shiyang Sun, Kelei Li, Fan Gao, Lianlian Bian, Ce Yang, Bopei Cui, Longfa Xu, Tong Cheng, Zhenglun Liang Human Vaccines & Immunotherapeutics.2019; 15(10): 2343. CrossRef
Corni Fructus: a review of chemical constituents and pharmacological activities Yu Dong, Zhe-Ling Feng, Hu-Biao Chen, Fu-Sheng Wang, Jia-Hong Lu Chinese Medicine.2018;[Epub] CrossRef
Inhibition of Murine Norovirus and Feline Calicivirus by Edible Herbal Extracts Dong Joo Seo, Changsun Choi Food and Environmental Virology.2017; 9(1): 35. CrossRef
Antiviral activity of herbal extracts against the hepatitis A virus Dong Joo Seo, Minhwa Lee, Su Been Jeon, Hyunkyung Park, Suntak Jeong, Bog-Hieu Lee, Changsun Choi Food Control.2017; 72: 9. CrossRef
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Characterization of VP1 sequence of Coxsackievirus A16 isolates by Bayesian evolutionary method Guolian Zhao, Xun Zhang, Changmin Wang, Guoqing Wang, Fan Li Virology Journal.2016;[Epub] CrossRef
Traditional uses, phytochemistry, and pharmacology of the genus Acer (maple): A review Wu Bi, Ying Gao, Jie Shen, Chunnian He, Haibo Liu, Yong Peng, Chunhong Zhang, Peigen Xiao Journal of Ethnopharmacology.2016; 189: 31. CrossRef
Epidemiological Research on Hand, Foot, and Mouth Disease in Mainland China Zhi-Chao Zhuang, Zeng-Qiang Kou, Yong-Juan Bai, Xiang Cong, Li-Hong Wang, Chun Li, Li Zhao, Xue-Jie Yu, Zhi-Yu Wang, Hong-Ling Wen Viruses.2015; 7(12): 6400. CrossRef
Objectives
The aim of this study was to develop an immunochromatographic assay (ICA) for the detection of influenza A (H1N1) pdm09 virus infection.
Materials and methods
Several monoclonal antibodies against influenza A (H1N1) pdm09 virus were generated and an ICA (pdm09-ICA) was developed for the rapid and specific detection of influenza A (H1N1) pdm09 virus infection. The specificity and sensitivity of the developed assay were compared with that of hemagglutination assay and real-time reverse-transcription polymerase chain reaction (rRT-PCR). Results
The detection limit was estimated to be 1/2 (8) hemagglutinating unit; the sensitivity and specificity rates of pdm09-ICA were 75.86% (110/145) and 100% (43/43), respectively, compared with rRT-PCR. The cross-reactivity for 20 influenza viruses, including seasonal H1N1 viruses, was found to be negative except for the H1N1 virus (A/Swine/Korea/GC0503/2005). Conclusion
These results indicate that the proposed method can be easily used for rapid and specific detection of the pdm09 infection. The assay developed in this study would be a useful tool for distinguishing the pdm09 infection from seasonal influenza A and B infections.
Citations
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Sensitive detection of influenza a virus based on a CdSe/CdS/ZnS quantum dot-linked rapid fluorescent immunochromatographic test Anh Viet Thi Nguyen, Tung Duy Dao, Tien Thi Thuy Trinh, Du-Young Choi, Seung-Taek Yu, Hyun Park, Seon-Ju Yeo Biosensors and Bioelectronics.2020; 155: 112090. CrossRef
Detecting Influenza A (H1N1) pdm09 Virus Infection Using Immunochromatographic Assay Viroj Wiwanitkit Osong Public Health and Research Perspectives.2014; 5(2): 115. CrossRef