Osong Public Health and Research Perspectives

Original Articles

Molecular Typing of Mycobacterium intracellulare Using Pulsed-Field Gel Electrophoresis, Variable-Number Tandem-Repeat Analysis, Mycobacteria Interspersed Repetitive-Unit-Variable-Number Tandem Repeat Typing, and Multilocus Sequence Typing: Molecular Characterization and Comparison of Each Typing Methods: Semi Jeon, Nara Lim, Seungjik Kwon, Taesun Shim, Misun Park, Bum-Joon Kim, Seonghan Kim; Osong Public Health Res Perspect. 2014;5(3):119-130. Published online June 30, 2014; DOI: https://doi.org/10.1016/j.phrp.2014.04.003

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Objectives
Mycobacterium intracellulare is the major causative agent of nontuberculous mycobacteria-related pulmonary infections. The strain typing of M. intracellulare is important for the treatment and control of its infections. We compared the discrimination capacity and effective value of four different molecular typing methods.
Methods
Antibiotic susceptibility testing, hsp65 and rpoB sequencing, pulsed-field gel electrophoresis (PFGE), multilocus sequence typing (MLST), mycobacteria interspersed repetitive-unit-variable-number tandem-repeat analysis (MIRU-VNTR), and VNTR assay targeting 44 M. intracellulare isolates obtained from patients with pulmonary infections were performed.
Results
All the antibiotic susceptibility patterns had no association with the molecular and sequence types tested in this study; however, the molecular and sequence types were related with each other. PFGE gave best results for discriminatory capacity, followed by VNTR, MLST, and MIRU-VNTR.
Conclusion
The high discriminatory power of PFGE, VNTR, and MLST is enough for differentiating between reinfection and relapse, as well as for other molecular epidemiological usages. The MLST could be regarded as a representative classification method, because it showed the clearest relation with the sequence types.

Citations

Citations to this article as recorded by

Applications and advances in molecular diagnostics: revolutionizing non-tuberculous mycobacteria species and subspecies identification
Haiyang Zhang, Maoting Tang, Deyuan Li, Min Xu, Yusen Ao, Liangkang Lin
Frontiers in Public Health.2024;[Epub] CrossRef
Differential Genotyping of Mycobacterium avium Complex and Its Implications in Clinical and Environmental Epidemiology
Jeong-Ih Shin, Sung Jae Shin, Min-Kyoung Shin
Microorganisms.2020; 8(1): 98. CrossRef
A strategy based on Amplified Fragment Length Polymorphism (AFLP) for routine genotyping of nontuberculous mycobacteria at the clinical laboratory
Sara Blanco-Conde, Carolina González-Cortés, Ramiro López-Medrano, Juan José Palacios-Gutiérrez, Cristina Diez-Tascón, Teresa Nebreda-Mayoral, María Josefa Sierra-García, Octavio Miguel Rivero-Lezcano
Molecular Biology Reports.2020; 47(5): 3397. CrossRef
Comparative Evaluation of Band-Based Genotyping Methods for Mycobacterium intracellulare and Its Application for Epidemiological Analysis
Jeong-Ih Shin, Jong-Hun Ha, Dong-Hae Lee, Jeong-Gyu Choi, Kyu-Min Kim, Seung Jun Lee, Yi Yeong Jeong, Jong Deog Lee, Myunghwan Jung, Seung-Chul Baik, Woo Kon Lee, Hyung-Lyun Kang, Min-Kyoung Shin, Jung-Wan Yoo
Microorganisms.2020; 8(9): 1315. CrossRef
Pulsed Field Gel Electrophoresis: Past, present, and future
Lilia Lopez-Canovas, Maximo B. Martinez Benitez, Jose A. Herrera Isidron, Eduardo Flores Soto
Analytical Biochemistry.2019; 573: 17. CrossRef
Molecular typing of Mycobacterium kansasii using pulsed-field gel electrophoresis and a newly designed variable-number tandem repeat analysis
Zofia Bakuła, Anna Brzostek, Paulina Borówka, Anna Żaczek, Izabela Szulc-Kiełbik, Agata Podpora, Paweł Parniewski, Dominik Strapagiel, Jarosław Dziadek, Małgorzata Proboszcz, Jacek Bielecki, Jakko van Ingen, Tomasz Jagielski
Scientific Reports.2018;[Epub] CrossRef
Mycobacterium paraintracellulare sp. nov., for the genotype INT-1 of Mycobacterium intracellulare
So-Young Lee, Byoung-Jun Kim, Hong Kim, Yu-Seop Won, Che Ok Jeon, Joseph Jeong, Seon Ho Lee, Ji-Hun Lim, Seung-Heon Lee, Chang Ki Kim, Yoon-Hoh Kook, Bum-Joon Kim
International Journal of Systematic and Evolution.2016; 66(8): 3132. CrossRef
Methodological and Clinical Aspects of the Molecular Epidemiology of Mycobacterium tuberculosis and Other Mycobacteria
Tomasz Jagielski, Alina Minias, Jakko van Ingen, Nalin Rastogi, Anna Brzostek, Anna Żaczek, Jarosław Dziadek
Clinical Microbiology Reviews.2016; 29(2): 239. CrossRef
Genetic diversity of clinical Mycobacterium avium subsp. hominissuis and Mycobacterium intracellulare isolates causing pulmonary diseases recovered from different geographical regions
Kazuya Ichikawa, Jakko van Ingen, Won-Jung Koh, Dirk Wagner, Max Salfinger, Takayuki Inagaki, Kei-ichi Uchiya, Taku Nakagawa, Kenji Ogawa, Kiyofumi Yamada, Tetsuya Yagi
Infection, Genetics and Evolution.2015; 36: 250. CrossRef

Multiplex Real-time Polymerase Chain Reaction Assays for Simultaneous Detection of Vibrio cholerae, Vibrio parahaemolyticus, and Vibrio vulnificus: Jie Yeun Park, Semi Jeon, Jun Young Kim, Misun Park, Seonghan Kim; Osong Public Health Res Perspect. 2013;4(3):133-139. Published online June 30, 2013; DOI: https://doi.org/10.1016/j.phrp.2013.04.004

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Abstract PDF

Objectives
A multiplex real-time polymerase chain reaction (RT-PCR) method was developed for the identification of three Vibrio species: Vibrio cholerae, Vibrio parahaemolyticus, and Vibrio vulnificus.
Methods
Specific primers and probes targeting the hlyA, tlh, and vvhA genes were selected and used for multiplex real-time PCR to confirm the identification of V. cholerae, V. parahaemolyticus, and V. vulnificus, respectively. This method was applied to screen Vibrio species from environmental samples and combining it with a culture-based method, its effectiveness was evaluated in comparison with culture-based methods alone.
Results
Specific PCR fragments were obtained from isolates belonging to the target species, indicating a high specificity of this multiplex real-time PCR. No cross-reactivity with the assay was observed between the tested bacteria. The sensitivity of the multiplex real-time PCR was found to have a lower limit of 10⁴ colony-forming units/reaction for all three Vibrio species. The combination strategy raised the isolation ratio of all three Vibrio species 1.26- to 2.75-fold.
Conclusion
This assay provides a rapid, sensitive, and specific technique to detect these three Vibrio species in the environment.

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Sharmin Quazi Bonny, M. A. Motalib Hossain, Syed Muhammad Kamal Uddin, Thiruchelvi Pulingam, Suresh Sagadevan, Mohd Rafie Johan
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Availability of Clean Tap Water and Medical Services Prevents the Incidence of Typhoid Fever: Deog-Yong Lee, Esther Lee, HyeMin Park, SeongHan Kim; Osong Public Health Res Perspect. 2013;4(2):68-71. Published online April 30, 2013; DOI: https://doi.org/10.1016/j.phrp.2013.03.005

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Abstract PDF

Objective: In this study, the factors that induced a decrease in the incidence of typhoid fever were analyzed. Based on the study results, we propose a quantitative and concrete solution to reduce the incidence of typhoid fever.
Methods
We analyzed the incidence and fatality rate of typhoid fever in Korea. Tap water service rate and the number of pharmacies, which affect the incidence rate of typhoid fever, were used as environmental factors.
Results
To prevent typhoid fever in the community, it is necessary to provide clean tap water service to 35.5% of the population, with an individual requiring 173 L of clean water daily. Appropriate access to clean water (51% service coverage, 307 L) helped the population to maintain individual hygiene and food safety practices, which brought about a decrease in the incidence of typhoid fever, and subsequently a decrease in fatality rate, which was achieved twice. During the 8-year study period, the fatality rate decreased to 1% when the population has access to proper medical service.
Conclusion
The fatality rate was primarily affected by the availability of medical services as well as by the incidence of typhoid fever. However, an analysis of the study results showed that the incidence of typhoid fever was affected only by the availability of clean water through the tap water system.

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Article

Multiplex Real-Time Polymerase Chain Reaction-Based Method for the Rapid Detection of gyrA and parC Mutations in Quinolone-Resistant Escherichia coli and Shigella spp.: Junyoung Kim, Semi Jeon, Hyungjun Kim, Misun Park, Soobok Kim, Seonghan Kim; Osong Public Health Res Perspect. 2012;3(2):113-117. Published online June 30, 2012; DOI: https://doi.org/10.1016/j.phrp.2012.04.004

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Abstract PDF

Two real-time polymerase chain reaction assays were developed to detect mutations in codons 83 and 87 in gyrA and in codons 80 and 91 in parC, the main sites that causes quinolone resistance in pathogenic Escherichia coli and Shigella spp. isolates. These assays can be employed as a useful method for controlling infections caused by quinolone-resistant E coli and Shigella isolates.

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New concepts in diagnostics for infectious diarrhea
J A Platts-Mills, J Liu, E R Houpt
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