Objectives
Vaginitis still remains as a health issue in women. It is notable that Candida albicans producing biofilm is considered a microorganism responsible for vaginitis with hard to treat. Also, Peganum harmala was applied as an anti fungal in treatment for many infections in Iran. Therefore, this study goal to investigate the role of P. harmala in inhibition of biofilm formation in C. albicans. Methods
So, 27 C. albicans collected from women with Vaginitis, then subjected for biofilm formation assay. P. harmala was applied as antibiofilm formation in C. albicans. Results
Our results demonstrated that P. harmala in concentration of 12 μg/ml easily inhibited strong biofilm formation; while the concentrations of 10 and 6 μg/ml inhibited biofilm formation in moderate and weak biofilm formation C. albicans strains, respectively. Conclusion
Hence, the current study presented P. harmala as antibiofilm herbal medicine for C. albicans; but in vivo study suggested to be performed to confirm its effectiveness.
Citations
Citations to this article as recorded by
Effect of Peganum harmala Extract on Biofilm and Involved Gene Expression in Biofilm Production of Candida albicans Maryam Erfaninejad, Elham Aboualigalehdari, Mahnaz Fatahinia Jundishapur Journal of Microbiology.2023;[Epub] CrossRef
Antifungal Mechanisms of a Chinese Herbal Medicine, Cao Huang Gui Xiang, Against Candida Species Huizhen Yue, Xiaolong Xu, Shasha He, Xuran Cui, Yuhong Guo, Jingxia Zhao, Bing Peng, Qingquan Liu Frontiers in Pharmacology.2022;[Epub] CrossRef
An overview on ethnobotanico-pharmacological studies carried out in Morocco, from 1991 to 2015: Systematic review (part 1) Jamila Fakchich, Mostafa Elachouri Journal of Ethnopharmacology.2021; 267: 113200. CrossRef
Improving health benefits with considering traditional and modern health benefits of Peganum harmala Mohamad Hesam Shahrajabian, Wenli Sun, Qi Cheng Clinical Phytoscience.2021;[Epub] CrossRef
Phytomedicine from Middle Eastern Countries: An Alternative Remedy to Modern Medicine against Candida spp Infection Mohammad Zubair Alam, Mohd Sajjad Ahmad Khan, Jenny Wilkinson Evidence-Based Complementary and Alternative Medic.2021; 2021: 1. CrossRef
In vitro and in vivo activity of Peganum harmala L. alkaloids against phytopathogenic bacteria Hanan A. Shaheen, Marwa Y. Issa Scientia Horticulturae.2020; 264: 108940. CrossRef
Molecules and Metabolites from Natural Products as Inhibitors of Biofilm in Candida spp. pathogens Rajeev K. Singla, Ashok K. Dubey Current Topics in Medicinal Chemistry.2019; 19(28): 2567. CrossRef
Assessment of the Antimicrobial and Antioxidant Activities of Ziziphus lotus and Peganum harmala Leila Ait Abderrahim, Khaled Taïbi, Chahinez Ait Abderrahim Iranian Journal of Science and Technology, Transac.2019; 43(2): 409. CrossRef
Evaluation of Biofilm Formation and Anti-biofilm Properties of Peganum Harmala and Crocus Sativus in Shigella Flexneri Clinical Isolates Mahsa Jalili, Mansour Amraei, Nourkhoda Sadeghifard, Sobhan Ghafourian The Open Microbiology Journal.2019; 13(1): 297. CrossRef
PgTeL, the lectin found in Punica granatum juice, is an antifungal agent against Candida albicans and Candida krusei Pollyanna Michelle da Silva, Maiara Celine de Moura, Francis Soares Gomes, Danielle da Silva Trentin, Ana Patrícia Silva de Oliveira, Gabriela Souto Vieira de Mello, Maira Galdino da Rocha Pitta, Moacyr Jesus Barreto de Melo Rego, Luana Cassandra Breitenb International Journal of Biological Macromolecules.2018; 108: 391. CrossRef
Antibiofilm activities of norharmane and its derivatives against Escherichia coli O157:H7 and other bacteria Jin-Hyung Lee, Yong-Guy Kim, Sang Hee Shim, Jintae Lee Phytomedicine.2017; 36: 254. CrossRef
Synthesis of silver nanoparticles using Peganum harmala extract as a green route Matin Azizi, Sajjad Sedaghat, Kambiz Tahvildari, Pirouz Derakhshi, Ahad Ghaemi Green Chemistry Letters and Reviews.2017; 10(4): 420. CrossRef
Cell cultures are developed from tissue samples and then disaggregated by mechanical, chemical, and enzymatic methods to extract cells suitable for isolation of viruses. With the recent advances in technology, cell culture is considered a gold standard for virus isolation. This paper reviews the evolution of cell culture methods and demonstrates why cell culture is a preferred method for identification of viruses. In addition, the advantages and disadvantages of both traditional and modern cell culture methods for diagnosis of each type of virus are discussed. Detection of viruses by the novel cell culture methods is considered more accurate and sensitive. However, there is a need to include some more accurate methods such as molecular methods in cell culture for precise identification of viruses.
Citations
Citations to this article as recorded by
The cell cultures in virology: from the past to the future Tatyana A. Kuznetsova, Natalia N. Besednova, Maxim R. Aliev, Michail Y. Shchelkanov Journal of microbiology, epidemiology and immunobi.2024; 101(1): 143. CrossRef
Role and significance of virus–bacteria interactions in disease progression Vatsala Pokhrel, Bhusan K Kuntal, Sharmila S Mande Journal of Applied Microbiology.2024;[Epub] CrossRef
A deep dive into AI integration and advanced nanobiosensor technologies for enhanced bacterial infection monitoring Habib Khan, Zahoor Jan, Inam Ullah, Abdullah Alwabli, Faisal Alharbi, Shabana Habib, Muhammad Islam, Byung-Joo Shin, Mi Young Lee, JaKeoung Koo Nanotechnology Reviews.2024;[Epub] CrossRef
Prospects of Acoustic Sensor Systems for Virus Immunodetection O. I. Guliy, B. D. Zaitsev, O. A. Karavaeva, I. A. Borodina Applied Biochemistry and Microbiology.2024; 60(5): 794. CrossRef
Identification and Characterization of Four Novel Viruses in Balclutha incisa Jiajing Xiao, Guang Yang, Renyi Liu, Danfeng Ge Insects.2024; 15(10): 772. CrossRef
Smartphone recognition-based immune microparticles for rapid on-site visual data-sharing detection of Newcastle disease virus Shenjunjie Lu, Binglin Ma, Haoran Zhou, Yayang Li, Zhihao Qiao, Tong Xiao, Husheng Li, Baijie Wang, Meihui Cui, Shufang Zhang, Jin Chang, Taofeng Du, Jing Liu, Hanjie Wang Talanta.2023; 252: 123845. CrossRef
Microbiological quality of irrigation water for cultivation of fruits and vegetables: An overview of available guidelines, water testing strategies and some factors that influence compliance. Oluwadara Alegbeleye, Anderson S. Sant’Ana Environmental Research.2023; 220: 114771. CrossRef
Raman spectroscopy for viral diagnostics Jijo Lukose, Ajaya Kumar Barik, Mithun N, Sanoop Pavithran M, Sajan D. George, V. M. Murukeshan, Santhosh Chidangil Biophysical Reviews.2023; 15(2): 199. CrossRef
Characterisation of new animal cell cultures’ sensitivity to Coxsackievirus B5 and Herpes simplex virus‑1 Yu. A. Zakharova, A. V. Ostapchuk, W. W. Wasielewski, O. S. Fedotova, N. A. Shmeleva Biological Products. Prevention, Diagnosis, Treatm.2023; 23(1): 102. CrossRef
Paper-based diagnostic chips for viral detection Srividya Oruganti, Sai Lakshmi Gundimeda, Viswanath Buddolla, Buddolla Anantha Lakshmi, Young-Joon Kim Clinica Chimica Acta.2023; 546: 117413. CrossRef
Automated System for Multiplexing Detection of COVID-19 and Other Respiratory Pathogens Parker Y. L. Tsang, Sunny L. H. Chu, Libby C. W. Li, Deborah M. S. Tai, Berry K. C. Cheung, Firaol Tamiru Kebede, Pete Y. M. Leung, Winston Wong, Teresa Chung, Cyril C. Y. Yip, Rosana W. S. Poon, Jonathan H. K. Chen, Kwok-Yung Yuen, Manson Fok, Johnson Y. IEEE Journal of Translational Engineering in Healt.2023; 11: 424. CrossRef
The Role of Viral Infection in the Pathogenesis of Interstitial Cystitis/Bladder Pain Syndrome Jia-Fong Jhang, Hann-Chorng Kuo Current Bladder Dysfunction Reports.2023; 18(4): 374. CrossRef
Isolation and pathogenic characterization of duck adenovirus 3 mutant circulating in China Xinjin Shi, Xinyu Zhang, Haiwei Sun, Changqing Wei, Yingnan Liu, Jiguan Luo, Xuebo Wang, Zongyan Chen, Hongjun Chen Poultry Science.2022; 101(1): 101564. CrossRef
Nanotechnology: A Potential Weapon to Fight against COVID‐19 Atul K. Tiwari, Anupa Mishra, Govind Pandey, Munesh K. Gupta, Prem C. Pandey Particle & Particle Systems Characterization.2022;[Epub] CrossRef
Establishment and characterization of a cell line from ictalurid catfish Suja Aarattuthodi, Vandana Dharan, Lester Khoo, Brian Bosworth Journal of the World Aquaculture Society.2022; 53(3): 620. CrossRef
Canine parvovirology – A brief updated review on structural biology, occurrence, pathogenesis, clinical diagnosis, treatment and prevention Deepika Tuteja, Kauser Banu, Bhairab Mondal Comparative Immunology, Microbiology and Infectiou.2022; 82: 101765. CrossRef
A review on the contamination of SARS-CoV-2 in water bodies: Transmission route, virus recovery and recent biosensor detection techniques Siti Adibah Zamhuri, Chin Fhong Soon, Anis Nurashikin Nordin, Rosminazuin Ab Rahim, Naznin Sultana, Muhammad Arif Khan, Gim Pao Lim, Kian Sek Tee Sensing and Bio-Sensing Research.2022; 36: 100482. CrossRef
State-of-the-art nanotechnologies used in the development of SARS-CoV-2 biosensors: a review Dongtak Lee, Taeha Lee, Ji Hye Hong, Hyo Gi Jung, Sang Won Lee, Gyudo Lee, Dae Sung Yoon Measurement Science and Technology.2022; 33(6): 062002. CrossRef
Viral culture and immunofluorescence for the detection of SARS-CoV-2 infectivity in RT-PCR positive respiratory samples Carla Berengua, Marina López, Montserrat Esteban, Pilar Marín, Paula Ramos, Margarita del Cuerpo, Ignasi Gich, Ferran Navarro, Elisenda Miró, Núria Rabella Journal of Clinical Virology.2022; 152: 105167. CrossRef
Genome-Wide Analysis and Molecular Characterization of Orf Virus Strain UPM/HSN-20 Isolated From Goat in Malaysia Hassana Kyari Mangga, Jamilu Abubakar Bala, Krishnan Nair Balakrishnan, Alhaji Modu Bukar, Zaharaddeen Lawan, Auwal Gambo, Faez Firdaus Abdullah Jesse, Mustapha M. Noordin, Mohd-Lila Mohd-Azmi Frontiers in Microbiology.2022;[Epub] CrossRef
Non-invasive detection of COVID-19 using a microfluidic-based colorimetric sensor array sensitive to urinary metabolites Mohammad Mahdi Bordbar, Hosein Samadinia, Azarmidokht Sheini, Jasem Aboonajmi, Mohammad Javid, Hashem Sharghi, Mostafa Ghanei, Hasan Bagheri Microchimica Acta.2022;[Epub] CrossRef
How Metagenomics Has Transformed Our Understanding of Bacteriophages in Microbiome Research Laura K. Inglis, Robert A. Edwards Microorganisms.2022; 10(8): 1671. CrossRef
Emergence of infectious diseases and role of advanced nanomaterials in point-of-care diagnostics: a review Kalaimani Markandan, Yong Wei Tiong, Revathy Sankaran, Sakthinathan Subramanian, Uma Devi Markandan, Vishal Chaudhary, Arshid Numan, Mohammad Khalid, Rashmi Walvekar Biotechnology and Genetic Engineering Reviews.2022; : 1. CrossRef
Respiratory Tract Infections and Laboratory Diagnostic Methods: A Review with A Focus on Syndromic Panel-Based Assays Adriana Calderaro, Mirko Buttrini, Benedetta Farina, Sara Montecchini, Flora De Conto, Carlo Chezzi Microorganisms.2022; 10(9): 1856. CrossRef
An Overview of Laboratory Diagnosis of Central Nervous System Viral Infections Sharifah Aliah Diyanah Syed Hussin, Ang-Lim Chua, Hassanain Al-Talib, Shamala Devi Sekaran, Seok Mui Wang Journal of Pure and Applied Microbiology.2022; 16(4): 2225. CrossRef
Multiplex detection of meningitis and encephalitis pathogens: A study from laboratory to clinic Yanjun Si, Weijun He, Shuo Guo, Xiaohui Wang, Meng Tang, Binwu Ying, Minjin Wang Frontiers in Neurology.2022;[Epub] CrossRef
Pathogenic Virus Detection by Optical Nanobiosensors Menglin Song, Mo Yang, Jianhua Hao Cell Reports Physical Science.2021; 2(1): 100288. CrossRef
Emerging antiviral therapeutics for human adenovirus infection: Recent developments and novel strategies Mackenzie J. Dodge, Katelyn M. MacNeil, Tanner M. Tessier, Jason B. Weinberg, Joe S. Mymryk Antiviral Research.2021; 188: 105034. CrossRef
Magnetic Nanomaterials in Microfluidic Sensors for Virus Detection: A Review Nahid Rezvani Jalal, Parvaneh Mehrbod, Shahla Shojaei, Hagar Ibrahim Labouta, Pooneh Mokarram, Abbas Afkhami, Tayyebeh Madrakian, Marek J. Los, Dedmer Schaafsma, Michael Giersig, Mazaher Ahmadi, Saeid Ghavami ACS Applied Nano Materials.2021; 4(5): 4307. CrossRef
Virus Detection: A Review of the Current and Emerging Molecular and Immunological Methods A. Cassedy, A. Parle-McDermott, R. O’Kennedy Frontiers in Molecular Biosciences.2021;[Epub] CrossRef
Nanobased Platforms for Diagnosis and Treatment of COVID-19: From Benchtop to Bedside Elham Bidram, Yasaman Esmaeili, Abbas Amini, Rossella Sartorius, Franklin R. Tay, Laleh Shariati, Pooyan Makvandi ACS Biomaterials Science & Engineering.2021; 7(6): 2150. CrossRef
Contagious ecthyma: how serious is the disease worldwide? Zaharaddeen Lawan, Jamilu Abubakar Bala, Alhaji Modu Bukar, Krishnan Nair Balakrishnan, Hassana Kyari Mangga, Faez Firdaus Jesse Abdullah, Mustapha Mohamed Noordin, Mohd Lila Mohd-Azmi Animal Health Research Reviews.2021; 22(1): 40. CrossRef
Recent Advances in Two-Dimensional Transition Metal Dichalcogenide Nanocomposites Biosensors for Virus Detection before and during COVID-19 Outbreak Ching Ying Katherine Lam, Qin Zhang, Bohan Yin, Yingying Huang, Hui Wang, Mo Yang, Siu Hong Dexter Wong Journal of Composites Science.2021; 5(7): 190. CrossRef
Viral Cultures for Coronavirus Disease 2019 Infectivity Assessment: A Systematic Review Tom Jefferson, Elisabeth A Spencer, Jon Brassey, Carl Heneghan Clinical Infectious Diseases.2021; 73(11): e3884. CrossRef
Severe acute respiratory syndrome-coronavirus 2 in domesticated animals and its potential of transmission: A meta-analysis Yos Adi Prakoso, Chylen Setiyo Rini, Yuli Purwandari Kristianingrum, Nurul Hidayah, Dyah Widhowati, Miarsono Sigit Veterinary World.2021; : 2782. CrossRef
Nucleic Acids Analytical Methods for Viral Infection Diagnosis: State-of-the-Art and Future Perspectives Emanuele Luigi Sciuto, Antonio Alessio Leonardi, Giovanna Calabrese, Giovanna De Luca, Maria Anna Coniglio, Alessia Irrera, Sabrina Conoci Biomolecules.2021; 11(11): 1585. CrossRef
ATeam technology for detecting early signs of viral cytopathic effect Karla Cristine C. DOYSABAS, Mami OBA, Tomoki ISHIBASHI, Hideki SHIBATA, Hitoshi TAKEMAE, Hiroshi SHIMODA, Ronald TARIGAN, Tetsuya MIZUTANI, Atsuo IIDA, Eiichi HONDO Journal of Veterinary Medical Science.2020; 82(3): 387. CrossRef
Advanced “lab-on-a-chip” to detect viruses – Current challenges and future perspectives Jianjian Zhuang, Juxin Yin, Shaowu Lv, Ben Wang, Ying Mu Biosensors and Bioelectronics.2020; 163: 112291. CrossRef
Bee Viruses: Routes of Infection in Hymenoptera Orlando Yañez, Niels Piot, Anne Dalmon, Joachim R. de Miranda, Panuwan Chantawannakul, Delphine Panziera, Esmaeil Amiri, Guy Smagghe, Declan Schroeder, Nor Chejanovsky Frontiers in Microbiology.2020;[Epub] CrossRef
Study on Adenovirus Infection in vitro with Nanoself-Assembling Peptide as Scaffolds for 3D Culture
Di-Shu Ao, Lu-Yao Gao, Jing-Han Gu, Jun-Hua Qiao, Huan Wang, Yan-Fei Liu, Hong Song International Journal of Nanomedicine.2020; Volume 15: 6327. CrossRef
Determining sensitivity of novel animal-derived cell cultures to clinical isolates of human enterovirus Echovirus 11 and Coxsackievirus B5 A. V. Alimov, O. S. Fedotova, N. A. Shmelyova, A. A. Bakharev, A. V. Rezaykin, P. S. Usoltseva, B. S. Imangaliyev, T. L. Bakhareva Medical alphabet.2020; (18): 17. CrossRef
Strength and Weakness of Molecular Identification Strategies Against Causative Viral Agent from Emerging COVID-19
Chunguang Cui, Kisoon Kim Journal of Bacteriology and Virology.2020; 50(2): 65. CrossRef
Isolation and characterization of duck adenovirus 3 circulating in China Shaohua Shi, Rongchang Liu, Chunhe Wan, Longfei Cheng, Zhen Chen, Guanghua Fu, Hongmei Chen, Qiuling Fu, Yu Huang Archives of Virology.2019; 164(3): 847. CrossRef
Bibliometric mapping of microbiology research topics (2012–16): a comparison by socioeconomic development and infectious disease vulnerability values Tahereh Dehdarirad, Hajar Sotudeh, Jonathan Freer FEMS Microbiology Letters.2019;[Epub] CrossRef
Advances in Diagnostic Approaches for Viral Etiologies of Diarrhea: From the Lab to the Field Yashpal Singh Malik, Atul Kumar Verma, Naveen Kumar, Nadia Touil, Kumaragurubaran Karthik, Ruchi Tiwari, Durlav Prasad Bora, Kuldeep Dhama, Souvik Ghosh, Maged Gomaa Hemida, Ahmed S. Abdel-Moneim, Krisztián Bányai, Anastasia N. Vlasova, Nobumichi Kobayash Frontiers in Microbiology.2019;[Epub] CrossRef
Nanoparticle-Based LDI-MS Immunoassay for the Multiple Diagnosis of Viral Infections Han-Wei Chu, Chao-Sung Lai, Jo-Yun Ko, Scott G. Harroun, Chiao-I Chuang, Robert Y. L. Wang, Binesh Unnikrishnan, Chih-Ching Huang ACS Sensors.2019; 4(6): 1543. CrossRef
Development of conventional and real time PCR assays for rapid species authentication of mammalian cell lines commonly used in veterinary diagnostic laboratories Amaresh Das, Lizhe Xu, Wei Jia Research in Veterinary Science.2019; 126: 170. CrossRef
Development of an in-situ hybridization assay using riboprobes for detection of viral haemorrhagic septicemia virus (VHSV) mRNAs in a cell culture model Syed Shariq Nazir Qadiri, Soo-Jin Kim, Rahul Krishnan, Jae-Ok Kim, Wi-Sik Kim, Myung-Joo Oh Journal of Virological Methods.2019; 264: 1. CrossRef
Real-time Pcr Versus Shell Vial Culture On Urine of Patients With Suspected Congenital Cytomegalovirus Infection Luana Coltella, Stefania Ranno, Giuseppe Pizzichemi, Livia Piccioni, Stefano Chiavelli, Andrea Onetti Muda, Carlo Concato Future Virology.2019; 14(9): 585. CrossRef
Isolation of the Human Cytomegalovirus from bodily fluids Sigrid Johanna Camacho Ortega, Sonia Del Pilar Bohorquez Avila, Myriam Lucia Velandia Romero, Jaime Eduardo Castellanos Parra Acta Biológica Colombiana.2019; 24(3): 520. CrossRef
Parechovirus A Detection by a Comprehensive Approach in a Clinical Laboratory Bao-Chen Chen, Jenn-Tzong Chang, Tsi-Shu Huang, Jih-Jung Chen, Yao-Shen Chen, Ming-Wei Jan, Tsung-Hsien Chang Viruses.2018; 10(12): 711. CrossRef
Aptamer immobilization on amino-functionalized metal–organic frameworks: an ultrasensitive platform for the electrochemical diagnostic of Escherichia coli O157:H7 Saeed Shahrokhian, Saba Ranjbar The Analyst.2018; 143(13): 3191. CrossRef
Development and evaluation of a nested-PCR assay for Senecavirus A diagnosis Cesar Feronato, Raquel A. Leme, Jaqueline A. Diniz, Alais Maria Dall Agnol, Alice F. Alfieri, Amauri A. Alfieri Tropical Animal Health and Production.2018; 50(2): 337. CrossRef
Diagnosis of Viral Infection Using Real-time Polymerase Chain Reaction Kyung-Ah Hwang, Ji Hoon Ahn, Jae-Hwan Nam Journal of Bacteriology and Virology.2018; 48(1): 1. CrossRef
Virological and Immunological Outcomes of Coinfections Naveen Kumar, Shalini Sharma, Sanjay Barua, Bhupendra N. Tripathi, Barry T. Rouse Clinical Microbiology Reviews.2018;[Epub] CrossRef
Laboratory Diagnosis of Respiratory Tract Infections in Children – the State of the Art Shubhagata Das, Sherry Dunbar, Yi-Wei Tang Frontiers in Microbiology.2018;[Epub] CrossRef
Diagnostic Testing of Neurologic Infections Prashanth S. Ramachandran, Michael R. Wilson Neurologic Clinics.2018; 36(4): 687. CrossRef
Overview of Trends in the Application of Metagenomic Techniques in the Analysis of Human Enteric Viral Diversity in Africa’s Environmental Regimes Cecilia Osunmakinde, Ramganesh Selvarajan, Timothy Sibanda, Bhekie Mamba, Titus Msagati Viruses.2018; 10(8): 429. CrossRef
Nanomaterial-based biosensors for detection of pathogenic virus Ahad Mokhtarzadeh, Reza Eivazzadeh-Keihan, Paria Pashazadeh, Maryam Hejazi, Nasrin Gharaatifar, Mohammad Hasanzadeh, Behzad Baradaran, Miguel de la Guardia TrAC Trends in Analytical Chemistry.2017; 97: 445. CrossRef
Plasma assisted surface treatments of biomaterials L. Minati, C. Migliaresi, L. Lunelli, G. Viero, M. Dalla Serra, G. Speranza Biophysical Chemistry.2017; 229: 151. CrossRef
Combined Proteomics/Genomics Approach Reveals Proteomic Changes of Mature Virions as a Novel Poxvirus Adaptation Mechanism Marica Grossegesse, Joerg Doellinger, Alona Tyshaieva, Lars Schaade, Andreas Nitsche Viruses.2017; 9(11): 337. CrossRef
A Lipidomics Approach in the Characterization of Zika-Infected Mosquito Cells: Potential Targets for Breaking the Transmission Cycle Carlos Fernando Odir Rodrigues Melo, Diogo Noin de Oliveira, Estela de Oliveira Lima, Tatiane Melina Guerreiro, Cibele Zanardi Esteves, Raissa Marques Beck, Marina Aiello Padilla, Guilherme Paier Milanez, Clarice Weis Arns, José Luiz Proença-Modena, Jayme PLOS ONE.2016; 11(10): e0164377. CrossRef
Objectives
In an attempt α-amylase gene from Pyrococcus woesei was amplified and cloned into a pTYB2 vector to generate the recombinant plasmid pTY- α-amylase. Methods Escherichia coli BL21 used as a host and protein expression was applied using IPTG. SDS-PAGE assay demonstrated the 100 kDa protein. Amylolytic activity of proteins produced by transformed E. coli cells was detected by zymography, and the rate of active α-amylase with and without the intein tag in both soluble conditions and as inclusion bodies solubilized by 4M urea were measured. Results
Amylolytic activity of ∼185,000 U/L of bacterial culture was observed from the soluble form of the protein using this system. Conclusion
These results indicate that this expression system was appropriate for the production of thermostable α-amylase.
Citations
Citations to this article as recorded by
Escherichia coli expression and characterization of α-amylase from Geobacillus thermodenitrificans DSM-465 A. Al-Amri, M. A. Al-Ghamdi, J. A. Khan, H. N. Altayeb, H. Alsulami, M. Sajjad, O. A. Baothman, M. S. Nadeem Brazilian Journal of Biology.2022;[Epub] CrossRef
Glycoside Hydrolases and Glycosyltransferases from Hyperthermophilic Archaea: Insights on Their Characteristics and Applications in Biotechnology Khadija Amin, Sylvain Tranchimand, Thierry Benvegnu, Ziad Abdel-Razzak, Hala Chamieh Biomolecules.2021; 11(11): 1557. CrossRef
Optimization, Purification, and Starch Stain Wash Application of Two Newα-Amylases Extracted from Leaves and Stems ofPergularia tomentosa Imen Lahmar, Hanen El Abed, Bassem Khemakhem, Hafedh Belghith, Ferjani Ben Abdallah, Karima Belghith BioMed Research International.2017; 2017: 1. CrossRef
Objectives
Widespread use of β-lactam antibiotics could cause resistance to this group of antibiotics in pathogenic bacteria through the production of the enzyme β-lactamases. The aim of this study is to determine the molecular detection of AmpC β-lactamases among clinical Escherichia coli isolated from Ilam hospitals in Ilam, Iran. Methods
One hundred and twelve clinical isolates of E. coli were collected from hospitalized patients and were identified by biochemical tests. They were evaluated for extended spectrum beta-lactamases (ESBLs) production, and the positive strains were subjected to AmpC enzymes; for detection of AmpC cluster genes, multiplex polymerase chain reaction was applied. Results
The analysis showed 62.5% of isolates were ESBLs positive and that five strains revealed the AmpC cluster genes. This is the first report of FOXM cluster genes in E. coli in Iran. Conclusion
Based on our results, the prevalence of AmpC β-lactamases is increasing in Iran, which caused failure in antibiotic therapy. So, the current study recommended the revision of antibiotic policy in Iranian hospitals.
Citations
Citations to this article as recorded by
Prevalence of ESBL and AmpC genes in E. coli isolates from urinary tract infections in the north of Iran M. Sadeghi, H. Sedigh Ebrahim-Saraie, A. Mojtahedi New Microbes and New Infections.2022; 45: 100947. CrossRef
Amp-CKlebsiella-Induced Chorioamnionitis and Associated Abnormalities in Cardiotocography: A Case Report with a Narrative Review Sufia Athar, Anvar P. Vellamgot, Lolwa Mohammed Alansari, Mohd Adnan Case Reports in Infectious Diseases.2022; 2022: 1. CrossRef
Molecular characteristics, antimicrobial resistance profiles, and antibiotic resistance determinants in uropathogenic fluoroquinolone resistant-Escherichia coli isolates Hassan Valadbeigi, Masoumeh HatamiLak, Abbas Maleki, Ebrahim Kouhsari, Nourkhoda Sadeghifard Gene Reports.2020; 18: 100584. CrossRef
High prevalence of blaCMY AmpC beta-lactamase in ESBL co-producing Escherichia coli and Klebsiella spp. clinical isolates in the northeast of Iran Kobra Salimiyan Rizi, Arman Mosavat, Masoud Youssefi, Saeid Amel Jamehdar, Kiarash Ghazvini, Hadi Safdari, Yousef Amini, Hadi Farsiani Journal of Global Antimicrobial Resistance.2020; 22: 477. CrossRef
Evaluating the antimicrobial resistance and frequency of AmpC β-lactamases bla gene in Gram-negative bacteria isolates collected from selected hospitals of Iran: A multicenter retrospective study Fatemeh Fallah, Taher Azimi, Leila Azimi, Abdollah Karimi, Mohammad Rahbar, Mehdi Shirdoust, Iraj Sedighi, Ata Saadat Sadeghi, Shahnaz Armin Gene Reports.2020; 21: 100868. CrossRef
Plasmid-Mediated AmpC β-Lactamase CITM and DHAM Genes Among Gram-Negative Clinical Isolates
Subhas Chandra Aryal, Milan Kumar Upreti, Anil Kumar Sah, Mehraj Ansari, Krishus Nepal, Binod Dhungel, Nabaraj Adhikari, Binod Lekhak, Komal Raj Rijal Infection and Drug Resistance.2020; Volume 13: 4249. CrossRef
Isolation and molecular characterization of multidrug-resistant Escherichia coli from chicken meat Md. Masudur Rahman, Asmaul Husna, Hatem A. Elshabrawy, Jahangir Alam, Nurjahan Yasmin Runa, A. T. M. Badruzzaman, Nahid Arjuman Banu, Mohammad Al Mamun, Bashudeb Paul, Shobhan Das, Md. Mahfujur Rahman, A. T. M. Mahbub-E-Elahi, Ahmed S. Khairalla, Hossam M Scientific Reports.2020;[Epub] CrossRef
PHENOTYPIC DETECTION OF AMPC β-LACTAMASE ENZYME IN GRAM-NEGATIVE BACILLI Khanda Anoar, Sherko Omer, Bayan Majid, Hero Rahim, Shno Muhammed JOURNAL OF SULAIMANI MEDICAL COLLEGE.2018; 8(2): 57. CrossRef
Bacteraemia due to AmpC β-lactamase-producing Escherichia coli in hospitalized cancer patients: risk factors, antibiotic therapy, and outcomes Qing Zhang, Wenfang Zhang, Zheng Li, Changsen Bai, Ding Li, Shan Zheng, Peng Zhang, Sihe Zhang Diagnostic Microbiology and Infectious Disease.2017; 88(3): 247. CrossRef