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Brief Report
Isolation and identification of monkeypox virus MPXV-ROK-P1-2022 from the first case in the Republic of Korea
Jin-Won Kim, Minji Lee, Hwachul Shin, Chi-Hwan Choi, Myung-Min Choi, Jee Woong Kim, Hwajung Yi, Cheon-Kwon Yoo, Gi-Eun Rhie
Osong Public Health Res Perspect. 2022;13(4):308-311.   Published online August 31, 2022
DOI: https://doi.org/10.24171/j.phrp.2022.0232
  • 7,798 View
  • 172 Download
  • 9 Web of Science
  • 9 Crossref
Graphical AbstractGraphical Abstract AbstractAbstract PDF
Objectives
Monkeypox outbreaks in nonendemic countries have been reported since early May 2022. The first case of monkeypox in the Republic of Korea was confirmed in a patient who traveled to Europe in June 2022, and an attempt was made to isolate and identify the monkeypox virus (MPXV) from the patient’s specimens.
Methods
Clinical specimens from the patient were inoculated in Vero E6 cells. The isolated virus was identified as MPXV by the observation of cytopathic effects on Vero E6 cells, transmission electron microscopy, conventional polymerase chain reaction (PCR), and sequencing of PCR products.
Results
Cytopathic effects were observed in Vero E6 cells that were inoculated with skin lesion swab eluates. After multiple passages from the primary culture, orthopoxvirus morphology was observed using transmission electron microscopy. In addition, both MPXV-specific (F3L and ATI) and orthopoxvirus-specific genes (A39R, B2R, and HA) were confirmed by conventional PCR and Sanger sequencing.
Conclusion
These results indicate the successful isolation and identification of MPXV from the first patient in the Republic of Korea. The isolated virus was named MPXV-ROK-P1-2022.

Citations

Citations to this article as recorded by  
  • Ultrasensitive one-pot detection of monkeypox virus with RPA and CRISPR in a sucrose-aided multiphase aqueous system
    Yue Wang, Yixin Tang, Yukang Chen, Guangxi Yu, Xue Zhang, Lihong Yang, Chenjie Zhao, Pei Wang, Song Gao, Frederick S. B. Kibenge, Ruijie Deng, Wei Chen, Shuang Yang
    Microbiology Spectrum.2024;[Epub]     CrossRef
  • Molecular Methods for Diagnosis of Monkeypox: A Mini-review
    Rodrigo Michelini de Oliveira Thomasi, Thais da Silva Correa, Dalise Silva do Carmo, Déborah Fernandes Rodrigues, Luiz Vinicius da Silva Correa, Sandra Rodrigues Xavier, Líria Souza Silva, Jonatas Oliveira da Silva, Michelli dos Santos, Alessandra da Silv
    Current Molecular Medicine.2024; 24(10): 1208.     CrossRef
  • Epidemiological, Clinical, and Virological Investigation of the First Four Cases of Monkeypox in Cartagena during the 2022 Outbreak
    Steev Loyola, Mashiel Fernández-Ruiz, Doris Gómez-Camargo
    Pathogens.2023; 12(2): 159.     CrossRef
  • 원숭이두창바이러스의 분리 배양과 전장유전체 정보 분석
    민지 이, 진원 김, 치환 최, 화철 신, 명민 최, 상은 이, 화중 이, 윤석 정
    Public Health Weekly Report.2023; 16(15): 464.     CrossRef
  • Overview of Diagnostic Methods, Disease Prevalence and Transmission of Mpox (Formerly Monkeypox) in Humans and Animal Reservoirs
    Ravendra P. Chauhan, Ronen Fogel, Janice Limson
    Microorganisms.2023; 11(5): 1186.     CrossRef
  • How to cope with suspected mpox patients in the outpatient clinic
    Nam Joong Kim, Sun Huh
    Journal of the Korean Medical Association.2023; 66(5): 325.     CrossRef
  • An Updated Review on Monkeypox Viral Disease: Emphasis on Genomic Diversity
    Ali Rabaan, Nada Alasiri, Mohammed Aljeldah, Abeer Alshukairiis, Zainab AlMusa, Wadha Alfouzan, Abdulmonem Abuzaid, Aref Alamri, Hani Al-Afghani, Nadira Al-baghli, Nawal Alqahtani, Nadia Al-baghli, Mashahed Almoutawa, Maha Mahmoud Alawi, Mohammed Alabdull
    Biomedicines.2023; 11(7): 1832.     CrossRef
  • Monkeypox (Mpox) virus isolation and ultrastructural characterisation from a Brazilian human sample case
    Milene Dias Miranda, Gabriela Cardoso Caldas, Vivian Neuza Ferreira, Ortrud Monika Barth, Aline de Paula Dias da Silva, Mayara Secco Torres Silva, Beatriz Grinsztejn, Valdiléa Gonçalves Veloso, Thiago Moreno Souza, Edson Elias da Silva, Debora Ferreira Ba
    Memórias do Instituto Oswaldo Cruz.2023;[Epub]     CrossRef
  • Isolation and Characterization of Monkeypox Virus from the First Case of Monkeypox — Chongqing Municipality, China, 2022
    Baoying Huang, Hua Zhao, Jingdong Song, Li Zhao, Yao Deng, Wen Wang, Roujian Lu, Wenling Wang, Jiao Ren, Fei Ye, Houwen Tian, Guizhen Wu, Hua Ling, Wenjie Tan
    China CDC Weekly.2022; 4(46): 1019.     CrossRef
Original Article
2019 Tabletop Exercise for Laboratory Diagnosis and Analyses of Unknown Disease Outbreaks by the Korea Centers for Disease Control and Prevention
Il-Hwan Kim, Jun Hyeong Jang, Su-Kyoung Jo, Jin Sun No, Seung-Hee Seo, Jun-Young Kim, Sang-Oun Jung, Jeong-Min Kim, Sang-Eun Lee, Hye-Kyung Park, Eun-Jin Kim, Jun Ho Jeon, Myung-Min Choi, Boyeong Ryu, Yoon Suk Jang, Hwami Kim, Jin Lee, Seung-Hwan Shin, Hee Kyoung Kim, Eun-Kyoung Kim, Ye Eun Park, Cheon-Kwon Yoo, Sang-Won Lee, Myung-Guk Han, Gi-Eun Rhie, Byung Hak Kang
Osong Public Health Res Perspect. 2020;11(5):280-285.   Published online October 22, 2020
DOI: https://doi.org/10.24171/j.phrp.2020.11.5.03
  • 6,472 View
  • 118 Download
AbstractAbstract PDF
Objectives

The Korea Centers for Disease Control and Prevention has published “A Guideline for Unknown Disease Outbreaks (UDO).” The aim of this report was to introduce tabletop exercises (TTX) to prepare for UDO in the future.

Methods

The UDO Laboratory Analyses Task Force in Korea Centers for Disease Control and Prevention in April 2018, assigned unknown diseases into 5 syndromes, designed an algorithm for diagnosis, and made a panel list for diagnosis by exclusion. Using the guidelines and laboratory analyses for UDO, TTX were introduced.

Results

Since September 9th, 2018, the UDO Laboratory Analyses Task Force has been preparing TTX based on a scenario of an outbreak caused by a novel coronavirus. In December 2019, through TTX, individual missions, epidemiological investigations, sample treatments, diagnosis by exclusions, and next generation sequencing analysis were discussed, and a novel coronavirus was identified as the causal pathogen.

Conclusion

Guideline and laboratory analyses for UDO successfully applied in TTX. Conclusions drawn from TTX could be applied effectively in the analyses for the initial response to COVID-19, an ongoing epidemic of 2019 – 2020. Therefore, TTX should continuously be conducted for the response and preparation against UDO.


PHRP : Osong Public Health and Research Perspectives
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