Objectives
To characterise the genetic and serological diversity of pathogenic Escherichia coli, we tested 111 E coli strains isolated from diarrhoeal patients in Korea between 2003 and 2006. Methods
The isolates were tested through polymerase chain reaction (PCR) and slide agglutination method for the detection of virulence genes and serotypes, respectively. To compare the expression of Shiga toxin (stx)-1 and stx2 genes, real-time quantitative reverse-transcriptase PCR and rapid exprssion assay, reversed-passive latex agglutination, were performed. Results
Forty-nine Shiga toxin-producing E coli (STEC) strains and 62 non-STEC strains, including 20 enteropathogenic E coli, 20 enterotoxigenic E coli, 20 enteroaggregative E coli, and 2 enteroinvasive E coli were randomly chosen from the strains isolated from diarrhoeal patients in Korea between 2003 and 2006. PCR analysis indicated that locus of enterocyte effacement pathogenicity island, that is, eaeA, espADB, and tir genes were present in STEC, enteropathogenic E coli, and enteroinvasive E coli. Quorum sensing-related gene luxS was detected in most of pathogenic E coli strains. Major serotypes of the STEC strains were O157 (26%) and O26 (20%), whereas the non-STEC strains possessed various serotypes. Especially, all the strains with serotype O157 carried stx2 and the tested virulence factors. Of the STEC strains, the data of real-time quantitative reverse-transcriptase PCR and reversed-passive latex agglutination tests showed that messenger RNA- and protein expression of stx2 gene were higher than those of stx1 gene. Conclusion
Our results provide the epidemiological information regarding the trend of STEC and non-STEC infections in the general population and show the fundamental data in association of serotypes with virulence genes in diarrhoeagenic E coli strains from Korea.
Citations
Citations to this article as recorded by
Comparative Analysis of Human and Animal E. coli: Serotyping, Antimicrobial Resistance, and Virulence Gene Profiling Mahmoud M. Bendary, Marwa I. Abdel-Hamid, Walaa A. Alshareef, Hanan M. Alshareef, Rasha A. Mosbah, Nasreen N. Omar, Mohammad M. Al-Sanea, Majid Alhomrani, Abdulhakeem S. Alamri, Walaa H. Moustafa Antibiotics.2022; 11(5): 552. CrossRef
Antimicrobial peptide human β-defensin-2 improves in vitro cellular viability and reduces pro-inflammatory effects induced by enteroinvasive Escherichia coli in Caco-2 cells by inhibiting invasion and virulence factors’ expression Alessandra Fusco, Vittoria Savio, Brunella Perfetto, Roberto Mattina, Giovanna Donnarumma Frontiers in Cellular and Infection Microbiology.2022;[Epub] CrossRef
Distribution of Pathogenicity Island Markers and H-Antigen Types of Escherichia coli O25b/ST131 Isolates from Patients with Urinary Tract Infection in Iran Masoumeh Rasoulinasab, Fereshteh Shahcheraghi, Mohammad Mehdi Feizabadi, Bahram Nikmanesh, Azade Hajihasani, Shahram Sabeti, Mohammad Mehdi Aslani Microbial Drug Resistance.2021; 27(3): 369. CrossRef
Development and validation of a predictive model for pathogenic
Escherichia coli
in fresh‐cut produce
You Jin Kim, Ju Yeon Park, Soo Hwan Suh, Mi‐Gyeong Kim, Hyo‐Sun Kwak, Soon Han Kim, Eun Jeong Heo Food Science & Nutrition.2021; 9(12): 6866. CrossRef
Occurrence of pathogenic Escherichia coli in commercially available fresh vegetable products in Korea Hyun Jung Kim, Minseon Koo, A-Ram Jeong, Seung-Youb Baek, Joon-Il Cho, Soon-Ho Lee, In-Gyun Hwang Journal of the Korean Society for Applied Biologic.2014; 57(3): 367. CrossRef