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Original Article
- Prevalence of plasmid-mediated AmpC β-lactamases among uropathogenic Escherichia coli isolates in southwestern Iran
- Nabi Jomehzadeh, Khadijeh Ahmadi, Zahra Rahmani
- Osong Public Health Res Perspect. 2021;12(6):390-395. Published online December 1, 2021
- DOI: https://doi.org/10.24171/j.phrp.2021.0272
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- 6 Web of Science
- 6 Crossref
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Abstract
PDF - Objectives
This study was undertaken to evaluate AmpC β-lactamase-producing Escherichia coli urine isolates and to characterize the frequency of plasmid-mediated AmpC (pAmpC)-encoding genes.
Methods
Antimicrobial susceptibility tests were performed using the disk diffusion technique. AmpC β-lactamase production was assessed with a phenotypic inhibitor-based method. The presence of 6 pAmpC-encoding cluster genes was detected by multiplex polymerase chain reaction (PCR).
Results
The proportion of antibiotic resistance of E. coli isolates ranged from 7.4% to 90.5%, and more than half (51.6%) of the total isolates were multidrug-resistant (MDR). Among the 95 E. coli isolates, 60 (63.2%) were found to be cefoxitin-resistant, but only 14 (14.7%) isolates were confirmed as AmpC β-lactamase-producers. In the PCR assay, pAmpC-encoding genes were found in 15 (15.8%) isolates, and blaDHA was the most prevalent type. However, blaFOX, blaMOX, and blaACC genes were not detected in the isolates.
Conclusion
Our findings contributed valuable information concerning antibiotic resistance, confirmatory phenotypic testing for AmpC production, and pAmpC β-lactamase gene content in E. coli isolates in southwestern Iran. The level of MDR recorded in AmpC-producing strains of this study was worrying; therefore, implementing strong infection control approaches to reduce the MDR burden is recommended. -
Citations
Citations to this article as recorded by
- A review of the mechanisms that confer antibiotic resistance in pathotypes of E. coli
Sina Nasrollahian, Jay P. Graham, Mehrdad Halaji
Frontiers in Cellular and Infection Microbiology.2024;[Epub] CrossRef - Association Between Uropathogenic Escherichia coli Virulence Genes and Severity of Infection and Resistance to Antibiotics
Sofía Alejandra Fonseca-Martínez, Ruth Aralí Martínez-Vega, Ana Elvira Farfán-García, Clara Isabel González Rugeles, Libeth Yajaira Criado-Guerrero
Infection and Drug Resistance.2023; Volume 16: 3707. CrossRef - Extended-spectrum β-lactamases producing Enterobacteriaceae (ESBL-PE) prevalence in Nepal: A systematic review and meta-analysis
Christina Khadka, Manita Shyaula, Gopiram Syangtan, Shrijana Bista, Reshma Tuladhar, Anjana Singh, Dev Raj Joshi, Lok R. Pokhrel, Prabin Dawadi
Science of The Total Environment.2023; 901: 166164. CrossRef - Prevalence of Extended-Spectrum β-Lactamase-Resistant Genes in Escherichia coli Isolates from Central China during 2016–2019
Zui Wang, Qin Lu, Xiaohui Mao, Li Li, Junfeng Dou, Qigai He, Huabin Shao, Qingping Luo
Animals.2022; 12(22): 3191. CrossRef - Molecular detection and characterization of Shigella spp. harboring extended-spectrum β-lactamase genes in children with diarrhea in northwest Iran
Sahar Sabour, Amir Teimourpour, Jafar Mohammadshahi, Hadi Peeridogaheh, Roghayeh Teimourpour, Taher Azimi, Zahra Hosseinali
Molecular and Cellular Pediatrics.2022;[Epub] CrossRef - Plasmid-mediated AmpC β-Lactamase Gene Analysis in Klebsiella Pneumoniae Clinical Isolates
Nabi Jomehzadeh, Khadijeh Ahmadi, Hasti Shaabaninejad, Gholamali Eslami
Biomedical and Biotechnology Research Journal (BBR.2022; 6(4): 582. CrossRef
- A review of the mechanisms that confer antibiotic resistance in pathotypes of E. coli
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