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Original Articles
Multiplex Real-time Polymerase Chain Reaction Assays for Simultaneous Detection of Vibrio cholerae, Vibrio parahaemolyticus, and Vibrio vulnificus
Jie Yeun Park, Semi Jeon, Jun Young Kim, Misun Park, Seonghan Kim
Osong Public Health Res Perspect. 2013;4(3):133-139.   Published online June 30, 2013
DOI: https://doi.org/10.1016/j.phrp.2013.04.004
  • 3,396 View
  • 34 Download
  • 22 Crossref
AbstractAbstract PDF
Objectives
A multiplex real-time polymerase chain reaction (RT-PCR) method was developed for the identification of three Vibrio species: Vibrio cholerae, Vibrio parahaemolyticus, and Vibrio vulnificus.
Methods
Specific primers and probes targeting the hlyA, tlh, and vvhA genes were selected and used for multiplex real-time PCR to confirm the identification of V. cholerae, V. parahaemolyticus, and V. vulnificus, respectively. This method was applied to screen Vibrio species from environmental samples and combining it with a culture-based method, its effectiveness was evaluated in comparison with culture-based methods alone.
Results
Specific PCR fragments were obtained from isolates belonging to the target species, indicating a high specificity of this multiplex real-time PCR. No cross-reactivity with the assay was observed between the tested bacteria. The sensitivity of the multiplex real-time PCR was found to have a lower limit of 104 colony-forming units/reaction for all three Vibrio species. The combination strategy raised the isolation ratio of all three Vibrio species 1.26- to 2.75-fold.
Conclusion
This assay provides a rapid, sensitive, and specific technique to detect these three Vibrio species in the environment.

Citations

Citations to this article as recorded by  
  • Vibrio cholerae and Salmonella Typhi culture-based wastewater or non-sewered sanitation surveillance in a resource-limited region
    Petros Chigwechokha, Ruth Lusungu Nyirenda, Davie Dalitsani, Ranken Lorvin Namaumbo, Yohanny Kazembe, Ted Smith, Rochelle H. Holm
    Journal of Exposure Science & Environmental Epidem.2024; 34(3): 432.     CrossRef
  • Epidemiological and Genomic analysis of Vibrio parahaemolyticus isolated from imported travelers at the port of Shanghai, China (2017-2019)
    Danlei Liu, Lei Zhou, Zilei Zhang, Ying Zhang, Zhiyi Wang, Shenwei Li, Yongqiang Zhu, Huajun Zheng, Zilong Zhang, Zhengan Tian
    BMC Microbiology.2024;[Epub]     CrossRef
  • Advances in Vibrio -related infection management: an integrated technology approach for aquaculture and human health
    Anshuman Mishra, Heui-Soo Kim, Rajender Kumar, Vaibhav Srivastava
    Critical Reviews in Biotechnology.2024; : 1.     CrossRef
  • First report of white muscle disease caused by Photobacterium damselae subsp. damselae in Kuruma shrimp (Penaeus japonicus)
    Garner Algo Langote Alolod, Sana Matsumoto, Keiichiro Koiwai, Hidehiro Kondo, Ikuo Hirono
    Aquaculture.2024; 593: 741278.     CrossRef
  • Incidence, virulence genes and antimicrobial resistance of Vibrio parahaemolyticus isolated from seafood
    Deyan Stratev, Rumyana Fasulkova, Gergana Krumova-Valcheva
    Microbial Pathogenesis.2023; 177: 106050.     CrossRef
  • Exploring the Effect of Functional Diets Containing Phytobiotic Compounds in Whiteleg Shrimp Health: Resistance to Acute Hepatopancreatic Necrotic Disease Caused by Vibrio parahaemolyticus
    Carla Hernández-Cabanyero, Esther Carrascosa, Silvia Jiménez, Belén Fouz
    Animals.2023; 13(8): 1354.     CrossRef
  • Multiplex PCR-Lateral Flow Dipstick Method for Detection of Thermostable Direct Hemolysin (TDH) Producing V. parahaemolyticus
    Jirakrit Saetang, Phutthipong Sukkapat, Suriya Palamae, Prashant Singh, Deep Nithun Senathipathi, Jirayu Buatong, Soottawat Benjakul
    Biosensors.2023; 13(7): 698.     CrossRef
  • Current trends in polymerase chain reaction based detection of three major human pathogenic vibrios
    Sharmin Quazi Bonny, M. A. Motalib Hossain, Syed Muhammad Kamal Uddin, Thiruchelvi Pulingam, Suresh Sagadevan, Mohd Rafie Johan
    Critical Reviews in Food Science and Nutrition.2022; 62(5): 1317.     CrossRef
  • Highly lethal Vibrio parahaemolyticus strains cause acute mortality in Penaeus vannamei post-larvae
    Feng Yang, Limei Xu, Wanzhen Huang, Fang Li
    Aquaculture.2022; 548: 737605.     CrossRef
  • Hepatopancreatic transcriptome analysis and humoral immune factor assays in red claw crayfish (Cherax quadricarinatus) provide insight into innate immunomodulation under Vibrio parahaemolyticus infection
    Duanduan Chen, Leifeng Guo, Cao Yi, Shouquan Wang, Yuanyuan Ru, Hui Wang
    Ecotoxicology and Environmental Safety.2021; 217: 112266.     CrossRef
  • Molecular mechanisms of Vibrio parahaemolyticus pathogenesis
    Lingzhi Li, Hongmei Meng, Dan Gu, Yang Li, Mengdie Jia
    Microbiological Research.2019; 222: 43.     CrossRef
  • Application of digital PCR and next generation sequencing in the etiology investigation of a foodborne disease outbreak caused by Vibrio parahaemolyticus
    Ying Li, Shuang Zhang, Jie Li, Meiling Chen, Mu He, Yuanyuan Wang, Yanchun Zhang, Hongbo Jing, Hongmei Ma, Yindong Li, Lin Zhao, Hongqun Zhao, Biao Kan, Bo Pang
    Food Microbiology.2019; 84: 103233.     CrossRef
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    Jeong Hyun Kim, Jin Lee, Sahyun Hong, Sangwon Lee, Hae-young Na, Young-Il Jeong, Eun Jin Choi, Junyoung Kim, Hyo Sun Kawk, Enhi Cho
    The American Journal of Tropical Medicine and Hygi.2018; 99(1): 168.     CrossRef
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    Deshun Xu, Lei Ji, Xiaofang Wu, Wei Yan, Liping Chen
    Canadian Journal of Microbiology.2018; 64(11): 809.     CrossRef
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    Sara Federici, Diana I. Serrazanetti, M. Elisabetta Guerzoni, Raffaella Campana, Eleonora Ciandrini, Wally Baffone, Andrea Gianotti
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  • First Case of Necrotizing Fasciitis Caused by Skermanella aerolata Infection Mimicking Vibrio Sepsis
    Sang Taek Heo, Ki Tae Kwon, Jeong Rae Yoo, Ji Young Choi, Keun Hwa Lee, Kwan Soo Ko
    Annals of Laboratory Medicine.2018; 38(6): 604.     CrossRef
  • Vibrio cholerae O1 with Reduced Susceptibility to Ciprofloxacin and Azithromycin Isolated from a Rural Coastal Area of Bangladesh
    Shah M. Rashed, Nur A. Hasan, Munirul Alam, Abdus Sadique, Marzia Sultana, Md. Mozammel Hoq, R. Bradley Sack, Rita R. Colwell, Anwar Huq
    Frontiers in Microbiology.2017;[Epub]     CrossRef
  • A label-free multi-functionalized graphene oxide based electrochemiluminscence immunosensor for ultrasensitive and rapid detection of Vibrio parahaemolyticus in seawater and seafood
    Yuhong Sha, Xuan Zhang, Wenrou Li, Wei Wu, Sui Wang, Zhiyong Guo, Jun Zhou, Xiurong Su
    Talanta.2016; 147: 220.     CrossRef
  • Toxigenic Vibrio cholerae O1 in vegetables and fish raised in wastewater irrigated fields and stabilization ponds during a non-cholera outbreak period in Morogoro, Tanzania: an environmental health study
    Yaovi M. G. Hounmanou, Robinson H. Mdegela, Tamègnon V. Dougnon, Ofred J. Mhongole, Edward S. Mayila, Joseph Malakalinga, George Makingi, Anders Dalsgaard
    BMC Research Notes.2016;[Epub]     CrossRef
  • Isolation of Vibrio vulnificus Biotype I from Disease Outbreaks on Cultured Tiger Grouper Epinephelus fuscoguttatus Forsskal, 1775
    Jumroensri Thawonsuwan, Jiraporn Kasornchandra, Patcharee Soonsan, Chantana Keawtapee
    Fish Pathology.2016; 51(Special-is): S39.     CrossRef
  • Detection of Vibrio cholerae by isothermal cross-priming amplification combined with nucleic acid detection strip analysis
    Xia Zhang, Xin-Jun Du, Chun Guan, Ping Li, Wen-Jie Zheng, Shuo Wang
    Molecular and Cellular Probes.2015; 29(4): 208.     CrossRef
  • lolB gene, a valid alternative for qPCR detection of Vibrio cholerae in food and environmental samples
    Alejandro Garrido-Maestu, María-José Chapela, Juan M. Vieites, Ana G. Cabado
    Food Microbiology.2015; 46: 535.     CrossRef
Genotypic Characterization of Vibrio vulnificus Clinical Isolates in Korea
Hye Sook Jeong, Jun Young Kim, Se Mi Jeon, Mi Sun Park, Seong Han Kim
Osong Public Health Res Perspect. 2011;2(1):8-14.   Published online June 30, 2011
DOI: https://doi.org/10.1016/j.phrp.2011.04.008
  • 2,979 View
  • 19 Download
  • 4 Crossref
AbstractAbstract PDF
Objectives
Vibrio vunificus is known to cause septicemia and severe wound infections in patients with chronic liver diseases or an immuno-compromised condition. We carried out the molecular characterization of V. vulnificus isolates from human Vibrio septicemia cases based on pulsed-field gel electrophoresis (PFGE) using NotI and SfiI.
Methods
and Results PFGE was used to characterize a total of 78 strains from clinical cases after NotI or SfiI digestion. The geographical distribution of PFGE patterns for the strains from the southern part of Korea, a high-risk region for Vibrio septicemia, indicated that the isolates from southeastern Korea showed a comparatively higher degree of homology than those from southwestern Korea.
Conclusions
We report the genetic distribution of V. vulnficus isolated from Vibrio septicemia cases during 2000–2004 in Korea. This method has potential use as a subspecies-typing tool for V. vulnificus strains isolated from distant geographic regions.

Citations

Citations to this article as recorded by  
  • Effect of Seawater Temperature Increase on the Occurrence of Coastal Vibrio vulnificus Cases: Korean National Surveillance Data from 2003 to 2016
    Jungsook Kim, Byung Chul Chun
    International Journal of Environmental Research an.2021; 18(9): 4439.     CrossRef
  • PCR-based evidence showing the presence of Vibrio vulnificus in wound infection cases in Mangaluru, India
    Caroline D’Souza, Ballamoole Krishna Kumar, Sachidananda Kapinakadu, Ranjith Shetty, Indrani Karunasagar, Iddya Karunasagar
    International Journal of Infectious Diseases.2018; 68: 74.     CrossRef
  • Genotypic Diversity and Population Structure of Vibrio vulnificus Strains Isolated in Taiwan and Korea as Determined by Multilocus Sequence Typing
    Hye-Jin Kim, Jae-Chang Cho, Paul J Planet
    PLOS ONE.2015; 10(11): e0142657.     CrossRef
  • The Road Less Traveled
    Chaeshin Chu
    Osong Public Health and Research Perspectives.2011; 2(1): 1.     CrossRef

PHRP : Osong Public Health and Research Perspectives