Objectives This study presents the standardized protocols developed by the Clinical-Based Human Microbiome Research and Development Project (cHMP) in the Republic of Korea.
Methods It addresses clinical metadata collection, specimen handling, DNA extraction, sequencing methods, and quality control measures for microbiome research.
Results The cHMP involves collecting samples from healthy individuals and patients across various body sites, including the gastrointestinal tract, oral cavity, respiratory system, urogenital tract, and skin. These standardized procedures ensure consistent data quality through controlled specimen collection, storage, transportation, DNA extraction, and sequencing. Sequencing encompasses both amplicon and whole metagenome methods, followed by stringent quality checks. The protocols conform to international guidelines, ensuring that the data generated are both reliable and comparable across microbiome studies.
Conclusion The cHMP underscores the importance of methodological standardization in enhancing data integrity, reproducibility, and advancing microbiome-based research with potential applications for improving human health outcomes.
Citations
Citations to this article as recorded by
Harnessing Dietary Tryptophan: Bridging the Gap Between Neurobiology and Psychiatry in Depression Management Amanda Chabrour Chehadi, Enzo Pereira de Lima, Cláudia Rucco Penteado Detregiachi, Rafael Santos de Argollo Haber, Virgínia Maria Cavallari Strozze Catharin, Lucas Fornari Laurindo, Vitor Engracia Valenti, Cristiano Machado Galhardi, Masaru Tanaka, Sandra International Journal of Molecular Sciences.2026; 27(1): 465. CrossRef
Human Microbiota-Associated Pig Models for Translational Microbiome Research: A Scoping Review Seong-Jin Wang, Hao-Yang Nian, Zhi-Hao Chen, Li Cui International Journal of Molecular Sciences.2026; 27(4): 1987. CrossRef
Gut microbiota-regulated tryptophan metabolism in breast cancer: mechanisms and therapeutic perspectives Jiaxi Yan, Linfeng Qian, Shiqi Chen, Maryam Mohammed Abbas Karekad, Xuanwei Wu, Musheng Xu, Xiaohong Xu Frontiers in Oncology.2026;[Epub] CrossRef
Genome-based medicine in Korea: the Korea National Institute of Health infrastructure for precision medicine Hyun-Young Park Childhood Kidney Diseases.2026; 30(1): 15. CrossRef
The gut microbiome as a biomarker and modifiable risk factor in Lynch Syndrome Verona Sarena Colaco, Annemarie Boleij Familial Cancer.2026;[Epub] CrossRef
The Expanding Landscape of Microbiota Medicine: Indications, Therapeutic Modalities, and the Path Towards Integrative Microbiome‐Targeting Healthcare Min Dai, Yisha Lin, Renyou Gan, Xiaotao Shen, Shin Young Park, Sunjae Lee, Qi Su Microbiota Medicine Research.2026;[Epub] CrossRef
First
Prev
