Osong Public Health and Research Perspectives

Volume 7(3); June 2016

Editorial

What Would Be a Better Strategy for National University Hospital Management?: Hae-Wol Cho, Chaeshin Chu; Osong Public Health Res Perspect. 2016;7(3):139-140. Published online June 30, 2016; DOI: https://doi.org/10.1016/j.phrp.2016.05.003

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Original Articles

Development of Financial Support Program for High Risk Pregnant Women: Ihnsook Jeong, Jiyun Kim, Sook Bin Im; Osong Public Health Res Perspect. 2016;7(3):141-148. Published online June 30, 2016; DOI: https://doi.org/10.1016/j.phrp.2016.02.003

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Abstract PDF: Objectives
The purpose of this study was to develop a financial support program for high-risk pregnant women based on opinions obtained using a questionnaire survey.
Methods
The program development involved two steps: (1) developing a questionnaire through reviewing previous financial support programs for maternal care and then validating it via professional consultation; and (2) drafting a financial support program. Sixty professionals, 26 high-risk pregnant women, and 100 program implementers completed the questionnaire between August 2014 and October 2014.
Results
Based on the obtained professional consultation and survey investigation, the framework of the financial support program was constructed. The suggested recipients were mothers with early labor pains, mothers who have been hospitalized for > 3 weeks, and mothers who used uterine stimulant Pitocin during hospitalization. All hospitalization, medication, and examination costs needed to be supported considering the income level of the recipient.
Conclusion
A basic policy for financially supporting high-risk pregnant women has been developed. The efficacy and feasibility of the policy needs to be carefully examined in future studies.

Competitive Strategy for Successful National University Hospital Management in the Republic of Korea: Munjae Lee; Osong Public Health Res Perspect. 2016;7(3):149-156. Published online June 30, 2016; DOI: https://doi.org/10.1016/j.phrp.2016.02.002

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Abstract PDF

Objectives
This study provides information to aid decision making for managers and the staff of national university hospitals through analyzing their financial statements.
Methods
In order to analyze the finances of national university hospitals, this study used the report of final accounts announced by each hospital from 2009 to 2012 as baseline data. The research participants were 10 national university hospitals.
Results
According to the results of the analysis, most hospitals (except for a few) had medical expenses exceeding their medical revenues, resulting in a net deficit; however, there were significant differences amongst the hospitals. The result of adjustments based on a standard size of 100 beds showed that most hospitals had medical revenue deficits, and there were significant differences between hospitals in terms of medical revenues and medical costs.
Conclusion
It is not clear whether an expansion of national university hospitals is always beneficial for increasing net revenues, and it is necessary to establish a differentiation strategy to increase profitability by securing financial soundness instead of externally-oriented growth.

Citations

Citations to this article as recorded by

A Study to Develop Sustainable Competitive Strategy for a Healthcare Organization in India
Priscilla Rejison
SSRN Electronic Journal.2024;[Epub] CrossRef
Internal Quality Process Management Evaluation in Higher Education by Students
Lucie Vnoučková, Hana Urbancová, Helena Smolová
Danube.2018; 9(2): 63. CrossRef
What Would Be a Better Strategy for National University Hospital Management?
Hae-Wol Cho, Chaeshin Chu
Osong Public Health and Research Perspectives.2016; 7(3): 139. CrossRef

Epidemiological and Clinical Features of People with Malta Fever in Iran: A Systematic Review and Meta-Analysis: Mahmood Moosazadeh, Roja Nikaeen, Ghasem Abedi, Motahareh Kheradmand, Saeid Safiri; Osong Public Health Res Perspect. 2016;7(3):157-167. Published online June 30, 2016; DOI: https://doi.org/10.1016/j.phrp.2016.04.009

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Abstract PDF

Objectives
Numerous studies have reported the epidemiological and clinical features of Malta fever incidence in Iran. Review and synthesis of the related literature through meta-analysis can provide an appropriate measurement for aforementioned indices. Therefore, the present study aimed to determine the epidemiological and clinical features of people with Malta fever in Iran.
Methods
The required documents were obtained through searching national and international databases. In each study, standard deviation of the indices was calculated using binomial distribution formulas. Finally, the heterogeneity index was determined between studies using Cochran (Q) and I² tests.
Results
Combining the results of 47 articles in the meta-analysis indicated that 57.6% (55.02–60.1%) and 42.3% (49.8–44.9%) of the patients were male and female, respectively. Most of the patients lived in rural areas; 68.4% (63.6–73.2%) compared to 31.4% (26.7–36.3%). In addition, 20.8% (17.4–24.2%) of the patients were ranchers and farmers, 16.9% (14.5–19.4%) were students, and 31.6% (27–36.2%) were housewives. Of the patients studies, 50.5% (35.6–65.2%) experienced contact with animals and 57.1% (46.4–67.9%) used unpasteurized dairy products. Fever, joint pain, and sweating were detected among 65.7% (53.7–77.8%) and 55.3% (44.4–66.2%), respectively.
Conclusion
The present study revealed that the frequency of male patients with brucellosis was considerably more than that of female patients. The number of patients with Malta fever in rural areas was significantly more than in urban areas. High-risk behavior, unprotected contact with animals, and using unpasteurized dairy products were among the most significant factors affecting Malta fever incidence in Iran. Fever, joint pain, and sweating were detected among most of the patients with Malta fever.

Citations

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A study of rural populations’ knowledge, attitude, and practice about brucellosis: a descriptive, cross-sectional, multicenter study
Zahra Montaseri, Zahra Mohebi, Rahil Masoumi, Azizallah Dehghan, Mostafa Bijani
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Brucellosis infection complicated with myelitis: a case report and literature review
Xiaoyu Ma, Ying Wang, Qiong Wu, Xiaomei Ma, Qiang Wang, Qinghong Guo
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Investigated of Epidemiological and Incidence of Human Brucellosis in Southwest of Iran, a Retrospective Study from 2014 to 2021
Parisa Asban, Fatemeh Kiani, Mohammad Javad Mohammadi, Saeed Ghanbari, Homayoon Amiri, Razieh Kazemi Bareh Bichast
Archives of Clinical Infectious Diseases.2024;[Epub] CrossRef
Diagnostic significance of hematological parameters in brucellosis
Mehmet Çelik, Mehmet Reşat Ceylan, Deniz Altındağ, Nevin Güler Dinçer, Sevil Alkan
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Presence of Brucella spp. in Milk and Dairy Products: A Comprehensive Review and Its Perspectives
Md. Sadequl Islam, Md. Ariful Islam, Md. Moshiur Rahman, Khaleda Islam, Md. Mominul Islam, Md. Murtuza Kamal, Md. Nazrul Islam, Gianfranco Picone
Journal of Food Quality.2023; 2023: 1. CrossRef
Predicting of Bacteremia in Patients with Brucellosis Using Machine Learning Methods
Mehmet ÇELİK, Mehmet Reşat CEYLAN, Deniz ALTINDAĞ, Sait Can YÜCEBAŞ, Nevin GÜLER DİNCER, Sevil ALKAN
Journal of Contemporary Medicine.2023; 13(3): 459. CrossRef
Toponyms in Dermatology
Heera Ramesh, Sachin Somashekar
Indian Journal of Dermatology.2022; 67(3): 279. CrossRef
Brucella pleurisy: An extremely rare complication of brucellosis
Ahmad Alikhani, Hamideh Abbaspour Kasgari, Haadi Majidi, Zahra Nekoukar
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Design and validation of brucellosis prevention questionnaire focused on animal vaccination
Farhad Bahadori, Fazlollah Ghofranipour, Saeideh Ghaffarifar, Reza Ziaei
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Forecasting the monthly incidence rate of brucellosis in west of Iran using time series and data mining from 2010 to 2019
Hadi Bagheri, Leili Tapak, Manoochehr Karami, Zahra Hosseinkhani, Hamidreza Najari, Safdar Karimi, Zahra Cheraghi, Esteban Tlelo-Cuautle
PLOS ONE.2020; 15(5): e0232910. CrossRef
Epidemiologically characteristics of human brucellosis and antimicrobial susceptibility pattern of Brucella melitensis in Hinggan League of the Inner Mongolia Autonomous Region, China
Hai-Tao Yuan, Cheng-Ling Wang, Li-Na Liu, Dan Wang, Dan Li, Zhen-Jun Li, Zhi-Guo Liu
Infectious Diseases of Poverty.2020;[Epub] CrossRef
Brucellosis: Evaluation of Two Hundred and Ten Cases with Different Clinical Features
Esma Eroglu, Bahar Kandemir
Annals of the Academy of Medicine, Singapore.2020; 49(7): 462. CrossRef
A comparison of three data mining time series models in prediction of monthly brucellosis surveillance data
Nasrin Shirmohammadi‐Khorram, Leili Tapak, Omid Hamidi, Zohreh Maryanaji
Zoonoses and Public Health.2019; 66(7): 759. CrossRef
Human brucellosis caused by raw dairy products: A review on the occurrence, major risk factors and prevention
Maryam Dadar, Youcef Shahali, Adrian M. Whatmore
International Journal of Food Microbiology.2019; 292: 39. CrossRef
Epidemiological, Clinical and Paraclinical Evaluation of Recorded Cases with Brucellosis in Kermanshah Province Health Center 2012 - 2016
Hossein Hatami, Ali Ramezankhani, Farahnaz Shekarchi
Journal of Kermanshah University of Medical Scienc.2019;[Epub] CrossRef
Epidemiological, risk factors, clinical, and laboratory features of brucellosis in the Southwest of Iran within 2009–2015
Mahmood Nabavi, Hossein Hatami, Hedayatollah Jamaliarand
International Journal of Preventive Medicine.2019; 10(1): 108. CrossRef
Meta-Analysis of the Changes of Peripheral Blood T Cell Subsets in Patients with Brucellosis
Rongjiong Zheng, Songsong Xie, Shaniya Niyazi, Xiaobo Lu, Lihua Sun, Yan Zhou, Yuexin Zhang, Kai Wang
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A Systematic Review and Meta-Analysis of Epidemiology and Clinical Manifestations of Human Brucellosis in China
Rongjiong Zheng, Songsong Xie, Xiaobo Lu, Lihua Sun, Yan Zhou, Yuexin Zhang, Kai Wang
BioMed Research International.2018; 2018: 1. CrossRef
The clinical features of 590 patients with brucellosis in Xinjiang, China with the emphasis on the treatment of complications
Bin Jia, Fengbo Zhang, Ying Lu, Wenbao Zhang, Jun Li, Yuexin Zhang, Jianbing Ding, Mazin Barry
PLOS Neglected Tropical Diseases.2017; 11(5): e0005577. CrossRef

Study on Seroprevalence and Leptospiral Antibody Distribution among High-risk Planters in Malaysia: J. Mohd Ridzuan, B.D. Aziah, W.M. Zahiruddin; Osong Public Health Res Perspect. 2016;7(3):168-171. Published online June 30, 2016; DOI: https://doi.org/10.1016/j.phrp.2016.04.006

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Abstract PDF

Objectives
To determine the leptospirosis seroprevalence and to identify the predominant infecting serovars among oil palm plantation workers.
Methods
The cross-sectional study involved 350 asymptomatic oil palm plantation workers in Melaka and Johor. A serological test using the microscopic agglutination test was conducted in the Institute of Medical Research with a cut-off titre for seropositivity of ≥1:100.
Results
The overall seroprevalence of leptospiral antibodies was 28.6%. The job category with the highest seroprevalence was the fruit collector with 59.2%. The predominant serovar identified was serovar Sarawak (Lepto 175) (62%).
Conclusion
A high seroprevalence of leptospiral antibodies was detected among oil palm plantation workers and specifically among fruit collectors. The predominant infecting serovar among the workers was serovar Sarawak (Lepto 175). The findings suggest that more studies are needed to determine the reasons for the high seroprevalence and the transmission and pathogenicity of the local serovar Sarawak (Lepto 175).

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Ekaterina V. Radyuk, Natalia V. Breneva, Sofia E. Budaeva, Marat T. Makenov, Olga А. Stukolova, Victoria P. Bulanenko, Lan Anh T. Le, Manh N. Dao, Chau V. Nguyen, Nga T. Bui Thi, Mo T. Luong, Tan N. Nguyen, Sergei V. Balakhonov, Lyudmila S. Karan
Acta Tropica.2024; 257: 107298. CrossRef
Leptospirosis in Vietnam
N. K. Tokarevich, O. V. Blinova
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Seroprevalence and serovar distribution of Leptospirosis among healthy people in Vietnam: Results from a multi-center study
Van Dinh Tran, Le Thi Phuong Mai, Nguyen Thi Thu, Bui Kim Linh, Phan Dang Than, Nguyen Tu Quyet, Luu Phuong Dung, Tran Ngoc Phuong Mai, Nguyen Thi My Hanh, Hoang Hai, Tran Nhu Duong, Dang Duc Anh
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Preventive Veterinary Medicine.2021; 197: 105525. CrossRef
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Bruno Ribeiro Rocha, Gabriel Martins, Walter Lilenbaum
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Aznida Mohamad Zaki, Rozita Hod, Nadia Aqilla Shamsusah, Zaleha Md Isa, Siti Khairani Bejo, Hani Kartini Agustar
Environmental Monitoring and Assessment.2020;[Epub] CrossRef
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Mohammad Saffree Jeffree, Daisuke Mori, Nur Athirah Yusof, Azman Bin Atil, Khamisah Awang Lukman, Rafidah Othman, Mohd Rohaizat Hassan, Lela Suut, Kamruddin Ahmed
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Kim R. Blasdell, Serge Morand, David Perera, Cadhla Firth, Mathieu Picardeau
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S. Samsudin, S. N. S. Sakinah, O. Malina, B. A. Norliza, M. A. Noh, A. Fairuz, T. Z. M. T. Jamaluddin, R. A. Hamat, W. M. Zahiruddin, S. Mohd Nazri, S. Sukeri, B. D. Aziah, I. Zawaha, A. W. Zainudin, N. A. Munirah, M. N. Desa, V. Neela, S. N. Masri
Tropical Medicine & International Health.2018; 23(3): 327. CrossRef
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Mas Harithulfadhli Agus Ab Rahman, Suhaily Mohd Hairon, Rukman Awang Hamat, Tengku Zetty Maztura Tengku Jamaluddin, Mohd Nazri Shafei, Norazlin Idris, Malina Osman, Surianti Sukeri, Zainudin A. Wahab, Wan Mohd Zahiruddin Wan Mohammad, Zawaha Idris, Aziah
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Chronic experimental genital leptospirosis with autochthonous Leptospira santarosai strains of serogroup Sejroe
Bruno Ribeiro Rocha, Mário Balaro, Paulo Victor Pereira, Gabriel Martins, Walter Lilenbaum
Small Ruminant Research.2018; 164: 28. CrossRef
Occurrence of Leptospira Species from Rodents, Soil and Water from an Oil Palm Plantation in Northern Sarawak
Lesley Maurice Bilung, Chai Fung Pui, Ahmad Syatir Tahar, Kasing Apun, Lela Su`ut, Yee Ling Chong, Jayasilan Mohd-Azlan
Asian Journal of Animal and Veterinary Advances.2018; 13(4): 332. CrossRef

Association between Pollen Risk Indexes, Air Pollutants, and Allergic Diseases in Korea: Hoseob Kim, Yoonhyung Park, Kwanjun Park, Byoungin Yoo; Osong Public Health Res Perspect. 2016;7(3):172-179. Published online June 30, 2016; DOI: https://doi.org/10.1016/j.phrp.2016.04.003

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Abstract PDF

Objectives
This study, different from the past researches, has been conducted in all age groups to understand the association between air pollutants, pollen risk indexes, and outpatients with allergic rhinitis (AR), asthma, and atopic dermatitis (AD).
Methods
Data on air pollutants, pollen risk indexes, and outpatients with each disease were collected from 2003 through 2011 to verify the association between them. All data are time-series materials that have been observed by time (day) and region, and are in a nonlinear shape. In particular, the total number of outpatients per day is a count data that had a Poisson distribution as the response variable. SAS 9.3 was used to make a statistical model, generalized additive model, with lag effects for the analysis.
Results
For allergic diseases during spring (April–May) and fall (September-October), a significant association was shown between the variables of air pollutants, pollens, and the number of outpatients. Especially, the estimates of NO₂ [AR (43.00967 ± 0.11284), asthma (52.01837 ± 0.06452), AD (52.01837 ± 0.06452), p < 0.001] in spring and SO₂ [AR (43.00967 ± 0.11284), asthma (52.01837 ± 0.06452), AD (52.01837 ± 0.06452), p < 0.001] in fall were highly significant and showed a positive association with all diseases.
Conclusion
Domestically and even internationally, various studies on the allergic diseases are being conducted. However, not many studies related to similar studies. In the need of creating grounds to back up these efforts, additional studies on allergic diseases, as well as researches utilizing pollen data, air pollution data, and claims data provided by the Health Insurance Corporation that has no problem in the representativeness of the data that have close relationships to the allergic disease will be needed.

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Aerobiologia.2024;[Epub] CrossRef
Study of the Interrelations of the Concentrations of Six Tree Pollen Types and Six Atmospheric Pollutants with Rhinitis and Allergic Conjunctivitis in the Community of Madrid
Javier Chico-Fernández, Esperanza Ayuga-Téllez
Applied Sciences.2024; 14(7): 2965. CrossRef
Trends in the Use of Air Quality Indexes in Asthma Studies
Angie Daniela Barrera-Heredia, Carlos Alfonso Zafra-Mejía, Alejandra Cañas Arboleda, María José Fernández Sánchez, Liliana López-Kleine, Adriana Rojas Moreno
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Fu-Mei Huang, Ci-Wen Luo, Shiuan-Shinn Lee, Yung-Chuan Ho, Yi-Ching Li, Yu-Chao Chang, Yu-Hsiang Kuan
Medicine.2023; 102(8): e32961. CrossRef
Air pollution and global healthcare use for atopic dermatitis: A systematic review
R. P. Fadadu, E. Chee, A. Jung, J. Y. Chen, K. Abuabara, M. L. Wei
Journal of the European Academy of Dermatology and.2023; 37(10): 1958. CrossRef
Associations of particulate matter with atopic dermatitis and chronic inflammatory skin diseases in South Korea
T. H. Park, S. Park, M. K. Cho, S. Kim
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Munsoo Han, Soo Jeong Choi, Yujin Jeong, Kijeong Lee, Tae Hoon Lee, Sang Hag Lee, Tae Hoon Kim
American Journal of Rhinology & Allergy.2022; 36(5): 649. CrossRef
Outdoor Air Pollution and Indoor Window Condensation Associated with Childhood Symptoms of Allergic Rhinitis to Pollen
Yingjie Liu, Chan Lu, Yuguo Li, Dan Norbäck, Qihong Deng
International Journal of Environmental Research an.2022; 19(13): 8071. CrossRef
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Intake Trends of Red Meat, Alcohol, and Fruits and Vegetables as Cancer-Related Dietary Factors from 1998 to 2009: Min Kyung Park, Hee Young Paik, Yeonsook Lee; Osong Public Health Res Perspect. 2016;7(3):180-189. Published online June 30, 2016; DOI: https://doi.org/10.1016/j.phrp.2016.04.005

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Abstract PDF

Objectives
Cancer is the leading cause of death worldwide, and cancer incidence and mortality have been increasing steadily in South Korea. This study aimed to examine the change in consumption of three cancer-related dietary factors—red meat, alcohol, and fruits/vegetables, and to evaluate consumption of these dietary factors among Koreans according to the criteria from the recommendations of the World Cancer Research Fund/American Institute of Cancer Research.
Methods
Consumption of red meat, alcoholic beverages, and fruits and vegetables was calculated from the 24-hour recall data of 36,486 individuals older than 20 years who were selected from the Korea National Health and Nutrition Examination Survey 1998–2009. The intake adequacy of these three factors was evaluated by the recommended criteria of the World Cancer Research Fund/American Institute of Cancer Research report.
Results
The mean red meat intake in the men in their 20s increased sharply (from 91.6 g to 111.3 g, p < 0.05). The mean alcohol intake increased continuously in men (from 10.3 g to 20.0 g, p < 0.05) and women (from 1.5 g to 3.5 g, p < 0.05). The mean fruit/vegetable intake decreased in the 21–29–year age group (from 349.4 g to 306.7 g in men; from 393.3 g to 292.5 g in women; p < 0.05). The percentage of individuals who did not meet the intake criteria for the three cancer-related dietary factors was especially high, and the percentage increased over 10 years in those in their 20s (p < 0.05).
Conclusion
We confirmed that intakes of red meat, alcoholic drink, and fruits and vegetables have moved toward a negative direction in both men and women in their 20s.

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The Mediating Role of Organizational Learning in the Relationship of Organizational Intelligence and Organizational Agility: Mohammad Amin Bahrami, Mohammad Mehdi Kiani, Raziye Montazeralfaraj, Hossein Fallah Zadeh, Morteza Mohammad Zadeh; Osong Public Health Res Perspect. 2016;7(3):190-196. Published online June 30, 2016; DOI: https://doi.org/10.1016/j.phrp.2016.04.007

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Abstract PDF

Objectives
Organizational learning is defined as creating, absorbing, retaining, transferring, and application of knowledge within an organization. This article aims to examine the mediating role of organizational learning in the relationship of organizational intelligence and organizational agility.
Methods
This analytical and cross-sectional study was conducted in 2015 at four teaching hospitals of Yazd city, Iran. A total of 370 administrative and medical staff contributed to the study. We used stratified-random method for sampling. Required data were gathered using three valid questionnaires including Alberkht (2003) organizational intelligence, Neefe (2001) organizational learning, and Sharifi and Zhang (1999) organizational agility questionnaires. Data analysis was done through R and SPSS 18 statistical software.
Results
The results showed that organizational learning acts as a mediator in the relationship of organizational intelligence and organizational agility (path coefficient = 0.943). Also, organizational learning has a statistical relationship with organizational agility (path coefficient = 0.382).
Conclusion
Our findings suggest that the improvement of organizational learning abilities can affect an organization's agility which is crucial for its survival.

Citations

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Comparing Performance Indicators of Obstetrics and Gynecology Ward at Yazd Educational Hospitals with Expected Limits of Indicators, 2015: Roohollah Askari, Milad Shafii, Najmeh Baghian; Osong Public Health Res Perspect. 2016;7(3):197-204. Published online June 30, 2016; DOI: https://doi.org/10.1016/j.phrp.2016.04.008

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Abstract PDF: Objectives
The purpose of this study was to evaluate the performance indicators for obstetrics and gynecology wards in Iran.
Methods
This study was designed as a cross-sectional study and was carried out in obstetrics and gynecology wards of Afshar and Shahid Sadoughi hospitals in 2015. The information required for the performance indicators was gathered through questionnaires, statistical forms, and direct observation. In several tables, performance rating and performance status are presented using the Likert scale index based on the expected limits.
Results
According to the results of the categories of input indicators, the bed occupancy rate of Afshar Hospital’s obstetrics and gynecology ward was, at 83%, higher than expected (79%), and that in Shahid Sadoughi Hospital (at 69%) was lower than expected. For medicinal methods and nonmedicinal methods of pain alleviation, the index process at Afshar Hospital was much lower than expected (40%). In Afshar Hospital, patient satisfaction at discharge was about 66.74%.
Conclusion
Effective steps can be taken to improve the input and output criteria: allocating appropriate physical space, examining the reasons for low bed occupancy rate by using complex analytical models, and in order to study the reasons for large number of cesarean section childbirth, it was recommended to place more emphasis on training of pregnant mothers and to inform them about the side effects of cesarean section and advantages of natural childbirth.

One-Step Reverse Transcription-Polymerase Chain Reaction for Ebola and Marburg Viruses: Sun-Whan Park, Ye-Ji Lee, Won-Ja Lee, Youngmee Jee, WooYoung Choi; Osong Public Health Res Perspect. 2016;7(3):205-209. Published online June 30, 2016; DOI: https://doi.org/10.1016/j.phrp.2016.04.004

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Abstract PDF

Objectives
Ebola and Marburg viruses (EBOVs and MARVs, respectively) are causative agents of severe hemorrhagic fever with high mortality rates in humans and nonhuman primates. In 2014, there was a major Ebola outbreak in various countries in West Africa, including Guinea, Liberia, Republic of Sierra Leone, and Nigeria. EBOV and MARV are clinically difficult to diagnose and distinguish from other African epidemic diseases. Therefore, in this study, we aimed to develop a method for rapid identification of the virus to prevent the spread of infection.
Methods
We established a conventional one-step reverse transcription-polymerase chain reaction (RT-PCR) assay for these pathogens based on the Superscript Reverse Transcriptase-Platinum Taq polymerase enzyme mixture. All assays were thoroughly optimized using in vitro-transcribed RNA.
Results
We designed seven primer sets of nucleocapsid protein (NP) genes based on sequences from seven filoviruses, including five EBOVs and two MARVs. To evaluate the sensitivity of the RT-PCR assay for each filovirus, 10-fold serial dilutions of synthetic viral RNA transcripts of EBOV or MARV NP genes were used to assess detection limits of viral RNA copies. The potential for these primers to cross react with other filoviruses was also examined. The results showed that the primers were specific for individual genotype detection in the examined filoviruses.
Conclusion
The assay established in this study may facilitate rapid, reliable laboratory diagnosis in suspected cases of Ebola and Marburg hemorrhagic fevers.

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