- The effect of photodynamic therapy using Radachlorin on biofilm-forming multidrug-resistant bacteria
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Choong-Won Seo, Young-Kwon Kim, Jeong-Lib An, Jong-Sook Kim, Pil-Seung Kwon, Young-Bin Yu
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Osong Public Health Res Perspect. 2022;13(4):290-297. Published online August 10, 2022
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DOI: https://doi.org/10.24171/j.phrp.2022.0150
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- Objectives
This study aimed to test the effect of photodynamic therapy (PDT) on the inhibition and removal of biofilms containing multidrug-resistant Acinetobacter baumannii.
Methods Using multidrug-resistant A. baumannii strains, an antibiotic susceptibility test was performed using the Gram-negative identification card of the Vitek 2 system (bioMérieux Inc., France), as well as an analysis of resistance genes, the effects of treatment with a light-emitting diode (LED) array using Radachlorin (RADA-PHARMA Co., Ltd., Russia), and transmission and scanning electron microscopy to confirm the biofilm-inhibitory effect of PDT.
Results The antibiotic susceptibility test revealed multiple resistance to the antibiotics imipenem and meropenem in the carbapenem class. A class-D–type β-lactamase was found, and OXA-23 and OXA-51 were found in 100% of 15 A. baumannii strains. After PDT using Radachlorin, morphological observations revealed an abnormal structure due to the loss of the cell membrane and extensive morphological changes, including low intracellular visibility and small vacuoles attached to the cell membrane.
Conclusion PDT involving a combination of LED and Radachlorin significantly eliminated the biofilm of multidrug-resistant A. baumannii. Observations made using electron microscopy showed that PDT combining LED and Radachlorin was effective. Additional studies on the effective elimination of biofilms containing multidrug-resistant bacteria are necessary, and we hope that a treatment method superior to sterilization with antibiotics will be developed in the future.
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Citations
Citations to this article as recorded by 
- Photophysical properties of Radachlorin photosensitizer in solutions of different pH, viscosity and polarity
A.V. Belashov, A.A. Zhikhoreva, I.A. Gorbunova, M.E. Sasin, Sh.S. Shayakhmedov, I.V. Semenova Spectrochimica Acta Part A: Molecular and Biomolec.2024; 305: 123480. CrossRef - Applications of Antimicrobial Photodynamic Therapy in Aquaculture: Effect on Fish Pathogenic Bacteria
Edith Dube, Grace Emily Okuthe Fishes.2024; 9(3): 99. CrossRef - Understanding the biofilm development of Acinetobacter baumannii and novel strategies to combat infection
Naji Naseef Pathoor, Akshaya Viswanathan, Gulshan Wadhwa, Pitchaipillai Sankar Ganesh APMIS.2024; 132(5): 317. CrossRef - PDT-Induced Variations of Radachlorin Fluorescence Lifetime in Living Cells In Vitro
Andrey V. Belashov, Anna A. Zhikhoreva, Anna V. Salova, Tatiana N. Belyaeva, Ilia K. Litvinov, Elena S. Kornilova, Irina V. Semenova Photonics.2023; 10(11): 1262. CrossRef
- Epidemiological Characterization of Imported Systemic Mycoses Occurred in Korea
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Seung-Hak Cho, Young-Bin Yu, Je-Seop Park, Keun-Dol Yook, Young-Kwon Kim
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Osong Public Health Res Perspect. 2018;9(5):255-260. Published online October 31, 2018
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DOI: https://doi.org/10.24171/j.phrp.2018.9.5.07
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11,830
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Objectives
Imported systemic mycoses is a severe fungal infection that can cause diseases in healthy people. However, there is a serious lack of epidemiological data about imported systemic mycoses. Therefore, an epidemiological characterization of imported systemic mycoses in Korea was performed.
Methods
We collected health insurance data between 2008 and 2012 from the Health Insurance Corporation and analyzed the data to determine the prevalence and treatment management of imported systemic mycoses.
Results
The prevalence of imported systemic mycoses between 2008 and 2012 increased slowly by 0.49/100,000 to 0.53/100,000 persons. The prevalence of coccidioidomycosis increased from 0.28/100,000 in 2008 to 0.36/100,000 persons in 2012. A mean of 229.6 cases occurred each year. Children and the elderly showed higher prevalence than adults in the 20- to 59-year-old age group. The rate of infection according to region ranged from 0.18/100,000 persons in Ulsan, to 0.59/100,000 persons in Gyeonggi. The prevalence in females was higher than that in males. Inpatient treatment was 3.3% (38 cases), with 96.7% treated as outpatients. Hospitalizations cost 272.7 million won and outpatient treatments cost 111.7 million won. The treatment cost for coccidioidomycosis from 2008 to 2012 was 330.9 million won, with personal charges of 79.2 million won and insurance charges of 251.7 million won. Most of the expenses for the coccidioidomycosis treatment were for inpatient treatment.
Conclusion
The results in this study may be a useful resource for determining the changes in the trend of imported systemic mycoses.
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- Trends in the Incidence of Scrub Typhus: The Fastest Growing Vector-Borne Disease in Korea
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Mi Ae Jeong, Seung-Ki Youn, Young-Kwon Kim, Hyungmin Lee, Sun-Ja Kim, Aeree Sohn
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Osong Public Health Res Perspect. 2013;4(3):166-169. Published online June 30, 2013
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DOI: https://doi.org/10.1016/j.phrp.2013.04.007
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3,788
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- Scrub typhus, also called tsutsugamushi disease, is classified as a Group 3 disease in Korea according to the National Notifiable Diseases Surveillance Systems. It is an infectious disease transmitted to humans through the bite of mites that are infected with an intracellular parasite called Orientia tsutsugamushi (Family: Rickettsiaceae). This study aims to identify the demographic characteristics of the infected cases according to profession, region, gender, and onset period and provide a basic data for prevention and control of the disease in the infected patients. Between 2001 and 2010, 16,741 men (36.3%) and 29,373 women (63.7%) were reported to have been infected with scrub typhus, with men being 1.6 times less infected than women. When classified according to age, it was found that 4421 persons (9.6%) were under 40 years of age; 6601 (13.1%) in their 40s; 9714 (21.1%) in their 50s; 13,067 (28.3%) in 60s; 10,128 (22.0%) in their 70s; and 2723 (5.9%) aged 80 or more. The elderly (60 years or older) represented more than half of the infected cases. When the infections were classified according to region, it was found that the county residents had the major share of infection, with a total of 1583 infected cases (59.85).
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Pei-Ying Peng, Hui-Ying Duan, Lei Xu, Lin-Tao Zhang, Ji-Qin Sun, Ya Zu, Li-Juan Ma, Yan Sun, Ting-Liang Yan, Xian-Guo Guo Scientific Reports.2024;[Epub] CrossRef - Identification of Bacteria and Viruses Associated with Patients with Acute Febrile Illness in Khon Kaen Province, Thailand
Rungrat Jitvaropas, Vorthon Sawaswong, Yong Poovorawan, Nutthanun Auysawasdi, Viboonsak Vuthitanachot, Sirima Wongwairot, Wuttikon Rodkvamtook, Erica Lindroth, Sunchai Payungporn, Piyada Linsuwanon Viruses.2024; 16(4): 630. CrossRef - Scrub typhus seroprevalence from an eastern state of India: findings from the state-wide serosurvey
Debaprasad Parai, Matrujyoti Pattnaik, Jaya Singh Kshatri, Usha Kiran Rout, Annalisha Peter, Rashmi Ranjan Nanda, Subrat Kumar Sahoo, Asit Mansingh, Hari Ram Choudhary, Girish Chandra Dash, Ira Praharaj, Debdutta Bhattacharya, Sanghamitra Pati Transactions of The Royal Society of Tropical Medi.2023; 117(1): 22. CrossRef - Epidemiological characteristics of cases with scrub typhus and their correlation with chigger mite occurrence (2019–2021): A focus on case occupation and activity locations
Se‐Jin Jeong, Jin‐Hwan Jeon, Kyung won Hwang Entomological Research.2023; 53(7): 247. CrossRef - Epidemiological Aspects of Tsutsugamushi Disease (Scrub Typhus) Outbreaks in Republic of Korea and Japan
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Kon Chu, Seon-Jae Ahn, Woo-Jin Lee, Jin-Sun Jun, Jung-Ah Lim, Keun-Hwa Jung, Kyung-Il Park, Jun-Sang Sunwoo, Han Sang Lee, Sang Kun Lee, Ki-Young Jung, Jangsup Moon, Soon-Tae Lee The American Journal of Tropical Medicine and Hygi.2017; 97(4): 1094. CrossRef - Generation of protective immunity against Orientia tsutsugamushi infection by immunization with a zinc oxide nanoparticle combined with ScaA antigen
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