Skip Navigation
Skip to contents

PHRP : Osong Public Health and Research Perspectives


Author index

Page Path
HOME > Articles and issues > Author index
Yoo-Hoon Lee 1 Article
Application of the Microagglutination Test for Serologic Diagnosis of Human Brucellosis
Sang-Hee Park, Yoo-Hoon Lee, Hyuk Chu, Seon-Do Hwang, Kyu-Jam Hwang, Hee-Yeol Choi, Mi-Yeoun Park
Osong Public Health Res Perspect. 2012;3(1):19-23.   Published online December 31, 2011
  • 3,046 View
  • 19 Download
  • 15 Crossref
AbstractAbstract PDF
Brucellosis is one of the most common zoonoses in the world, and occurs mainly in farmers, slaughterhouse workers, and veterinarians via direct or indirect contact with infected animals or their products. The clinical symptoms of human brucellosis are nonspecific, such as fever, headache, chills, and sweating. Diagnosis and treatment of brucellosis requires laboratory tests. Although the serum tube agglutination test (SAT) is the standardized gold method, it is laborious, time consuming, and requires a number of reagents. A microagglutination test (MAT) variant of the SAT or enzyme-linked immunosorbent assay (ELISA) is recommended for serological diagnoses. For the simple and rapid diagnosis of brucellosis, the MAT was standardized using samples for the SAT to define positive and negative categories, and we then compared the sensitivity and specificity of the MAT and ELISA.
Thirty SAT-positive sera and 60 SAT-negative sera were used in this study. Antibody titers of ≥1:160 were considered positive readings in both the SAT and MAT. Brucella abortus antigens and Brucella-positive control antiserum were used in the SAT and MAT. ELISAs of IgM and IgG were performed according to the manufacturers’ instructions.
The titers of the MAT differed according to antigen concentration. The optimal concentration of B abortus antigen was determined to compare the sensitivity and specificity between the MAT and SAT. The sensitivity and specificity of the MAT were 93.3% and 96.7%, respectively, for IgG with reference to ELISA, and 96.7% and 98.3%, respectively, for IgM.
The optimal concentration of antigen for the MAT was 1:10. The MAT is less time consuming and requires less antigen and serum than the SAT. The results of the MAT showed good agreement with those of ELISA. The results of this study suggest that the MAT could be useful for diagnosis of brucellosis.


Citations to this article as recorded by  
  • The Development of Diagnostic and Vaccine Strategies for Early Detection and Control of Human Brucellosis, Particularly in Endemic Areas
    Ayman Elbehiry, Musaad Aldubaib, Eman Marzouk, Adil Abalkhail, Abdulaziz M. Almuzaini, Mohammed Rawway, Ali Alghamdi, Abdullah Alqarni, Mohammed Aldawsari, Abdelmaged Draz
    Vaccines.2023; 11(3): 654.     CrossRef
  • New insights into the genetic predisposition of brucellosis and its effect on the gut and vaginal microbiota in goats
    Ahmed M. Sallam, Ibrahim Abou-souliman, Henry Reyer, Klaus Wimmers, Alaa Emara Rabee
    Scientific Reports.2023;[Epub]     CrossRef
  • Bovine brucellosis – a comprehensive review
    Sandip Kumar Khurana, Anju Sehrawat, Ruchi Tiwari, Minakshi Prasad, Baldev Gulati, Muhammad Zubair Shabbir, Rajesh Chhabra, Kumaragurubaran Karthik, Shailesh Kumar Patel, Mamta Pathak, Mohd. Iqbal Yatoo, Vivek Kumar Gupta, Kuldeep Dhama, Ranjit Sah, Wanpe
    Veterinary Quarterly.2021; 41(1): 61.     CrossRef
  • Microbiological Laboratory Diagnosis of Human Brucellosis: An Overview
    Giovanni Di Bonaventura, Silvia Angeletti, Andrea Ianni, Tommasangelo Petitti, Giovanni Gherardi
    Pathogens.2021; 10(12): 1623.     CrossRef
  • The spatiotemporal distribution of human brucellosis in mainland China from 2007-2016
    Peifeng Liang, Yuan Zhao, Jianhua Zhao, Dongfeng Pan, Zhongqin Guo
    BMC Infectious Diseases.2020;[Epub]     CrossRef
  • Brucellar spondylodiscitis: A case series with focus on histopathological features
    Soumaya Rammeh, Emna Romdhane, Hend Riahi, Meriem Ksentini, Mouna Chelli Bouaziz, Rahma Ayadi, Aida Berriche, Yosra Chebbi, Mohamed Fethi Ladeb
    Journal of Clinical Neuroscience.2020; 78: 360.     CrossRef
  • A case report of neurobrucellosis mimicking Guillain–Barré syndrome
    Parastoo Paydarnia, Soroush Moradi, Arman Habibi, Ladan Abbasian, Mojdeh Ghabaee
    Neurology, Psychiatry and Brain Research.2019; 31: 27.     CrossRef
  • A compact laser diode based photoacoustic spectral response technique to differentiate Brucellosis infected goat tissue from normal tissues
    A. Gorey, S. Shukla, J.G. Prasad, S. Verma, A. Sharma, S. Vasudevan
    Journal of Instrumentation.2019; 14(05): P05017.     CrossRef
  • Laboratory Diagnosis of Human Brucellosis
    Pablo Yagupsky, Pilar Morata, Juan D. Colmenero
    Clinical Microbiology Reviews.2019;[Epub]     CrossRef
  • Real-time PCR assays for diagnosing brucellar spondylitis using formalin-fixed paraffin-embedded tissues
    Man Li, Xingang Zhou, Jingjing Li, Lei Sun, Xiangmei Chen, Peng Wang
    Medicine.2018; 97(9): e0062.     CrossRef
  • Incidence of human brucellosis in the Kilimanjaro Region of Tanzania in the periods 2007–2008 and 2012–2014
    Manuela Carugati, Holly M Biggs, Michael J Maze, Robyn A Stoddard, Shama Cash-Goldwasser, Julian T Hertz, Jo E B Halliday, Wilbrod Saganda, Bingileki F Lwezaula, Rudovick R Kazwala, Sarah Cleaveland, Venance P Maro, Matthew P Rubach, John A Crump
    Transactions of The Royal Society of Tropical Medi.2018; 112(3): 136.     CrossRef
  • Seroreactivity and Risk Factors Associated with Human Brucellosis among Cattle Slaughterhouse Workers in South Korea
    Dilaram Acharya, Seon Do Hwang, Ji-Hyuk Park
    International Journal of Environmental Research an.2018; 15(11): 2396.     CrossRef
  • Outbreak of human brucellosis in Southern Brazil and historical review of data from 2009 to 2018
    Tamilly Silva Lemos, Juliana Clelia Cequinel, Tania Portela Costa, Amanda Boni Navarro, Andressa Sprada, Flávia Kazumi Shibata, Regina Gondolfo, Felipe Francisco Tuon, Stephen Baker
    PLOS Neglected Tropical Diseases.2018; 12(9): e0006770.     CrossRef
  • Brucellosis in Immunocompromised Hosts
    KA Al-Anazi, AM Al-Jasser
    Archives of Organ Transplantation.2016; 1(1): 001.     CrossRef
  • Evaluation and Selection of Multilocus Variable-Number Tandem-Repeat Analysis Primers for Genotyping Brucella abortus Biovar 1 Isolated from Human Patients
    Subok Lee, Kyu-Jam Hwang, Mi-Yeoun Park, Seon-Do Hwang, Hee-Youl Chai, Hyuk Chu, Sang-Hee Park
    Osong Public Health and Research Perspectives.2013; 4(5): 265.     CrossRef

PHRP : Osong Public Health and Research Perspectives