- Molecular characteristics of drug-susceptible Mycobacterium tuberculosis clinical isolates based on treatment duration
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Eon-Min Ko, Jinsoo Min, Hyungjun Kim, Ji-A Jeong, Sungkyoung Lee, Seonghan Kim
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Osong Public Health Res Perspect. 2024;15(5):385-394. Published online September 30, 2024
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DOI: https://doi.org/10.24171/j.phrp.2024.0101
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 Graphical Abstract
 Abstract
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 - Objectives
In this study, we performed comparative genomic and transcriptomic analysis of clinical isolates of Mycobacterium tuberculosis collected from patients with drug-susceptible tuberculosis (DS-TB). The clinical isolates were categorized based on treatment duration: standard 6 months or >6 months.
Methods
Study participants were recruited from a 2016 to 2018 tuberculosis cohort, and clinical M. tuberculosis isolates were collected from the sputum of patients with tuberculosis. We analyzed the genome and transcriptome of the isolated M. tuberculosis.
Results
Genomic analysis revealed a specific non-synonymous single-nucleotide polymorphism in pe_pgrs9 and ppe34, exclusive to the group treated for >6 months. Transcriptomic analysis revealed increased expression of various virulence-associated protein family genes and decreased expression of ribosomal protein genes and ppe38 genes in the group treated for >6 months.
Conclusion
The identified genetic variation and gene expression patterns may influence treatment outcomes by modulating host immune responses, increasing virulence, and potentially contributing to persister cell formation in M. tuberculosis. This study provides insights into the genetic and transcriptomic factors associated with prolonged DS-TB treatment. However, our study identified molecular characteristics using a small sample size, and further detailed studies are warranted.
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Citations
Citations to this article as recorded by  -
RNA extraction and RNA-sequencing method for transcriptomic analysis of
Mycobacterium tuberculosis
Morgan R. Hiebert, Meenu K. Sharma, Alwyn Go, Christine Bonner, Vanessa Laminman, Morag Graham, Hafid Soualhine
BioTechniques.2025; 77(1): 23. CrossRef
- Prevalent Multidrug-resistant Nonvaccine Serotypes in Pneumococcal Carriage of Healthy Korean Children Associated with the Low Coverage of the Seven-valent Pneumococcal Conjugate Vaccine
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Sungkyoung Lee, Ji-Hye Kim, Seong-Han Kim, Misun Park, Songmee Bae
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Osong Public Health Res Perspect. 2013;4(6):316-322. Published online December 31, 2013
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DOI: https://doi.org/10.1016/j.phrp.2013.10.004
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4,111
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Abstract
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- Objectives
Our previous longitudinal multicenter-based carriage study showed that the average carriage rate of Streptococcus pneumoniae was 16.8% in 582 healthy children attending kindergarten or elementary school in Seoul, Korea. We assessed serotype-specific prevalence and antimicrobial resistance among colonizing pneumococcal isolates from young children in the era of low use of the seven-valent pneumococcal conjugate vaccine (PCV7).
Methods
Serotypes were determined by an agglutination test with specific antisera or by a multiplex polymerase chain reaction (PCR) assay. An antimicrobial susceptibility test was performed with broth microdilution in Korean 96-well panels from Dade-MicroScan (Sacramento, CA, USA).
Results
Pneumococcal colonization patterns were dynamic and longterm persistent carriage was rare, which indicated a sequential turnover of pneumococcal strains. Of the 369 pneumococci (except for 23 killed isolates), 129 (34.9%) isolates were PCV7 vaccine serotypes (VTs); 213 (57.8%) isolates were nonvaccine serotypes (NVTs); and the remaining 27 (7.2%) isolates were nontypable (NT). The highest rates of multidrug resistance (MDR) were observed in VTs (86.0%; 111/129 isolates) and NVTs (70.0%; 149/213 isolates).
Conclusion
This study overall showed the frequent carriage of VTs and NVTs with MDR in healthy children attending kindergarten or elementary school. Efforts should be directed toward reducing the extensive prescription of antibiotics and using new broader vaccines to reduce the expansion of MDR strains of NVTs in our community.
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Citations
Citations to this article as recorded by - Characterization of Pneumococcal Colonization Dynamics and Antimicrobial Resistance Using Shotgun Metagenomic Sequencing in Intensively Sampled South African Infants
Rendani I. Manenzhe, Felix S. Dube, Meredith Wright, Katie Lennard, Stephanie Mounaud, Stephanie W. Lo, Heather J. Zar, William C. Nierman, Mark P. Nicol, Clinton Moodley
Frontiers in Public Health.2020;[Epub] CrossRef - Encouraging rational antibiotic use in childhood pneumonia: a focus on Vietnam and the Western Pacific Region
Nguyen T. K. Phuong, Tran T. Hoang, Pham H. Van, Lolyta Tu, Stephen M. Graham, Ben J. Marais
Pneumonia.2017;[Epub] CrossRef - Burden of bacterial upper respiratory tract pathogens in school children of Nepal
Sangita Thapa, Shishir Gokhale, Annavarapu Laxminarasimha Sharma, Lokendra Bahadur Sapkota, Shamshul Ansari, Rajendra Gautam, Sony Shrestha, Puja Neopane
BMJ Open Respiratory Research.2017; 4(1): e000203. CrossRef - Bacterial Density, Serotype Distribution and Antibiotic Resistance of Pneumococcal Strains from the Nasopharynx of Peruvian Children Before and After Pneumococcal Conjugate Vaccine 7
Christiane R. Hanke, Carlos G. Grijalva, Sopio Chochua, Mathias W. Pletz, Claudia Hornberg, Kathryn M. Edwards, Marie R. Griffin, Hector Verastegui, Ana I. Gil, Claudio F. Lanata, Keith P. Klugman, Jorge E. Vidal
Pediatric Infectious Disease Journal.2016; 35(4): 432. CrossRef
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