- Possibility of CTX-M-14 Gene Transfer from Shigella sonnei to a Commensal Escherichia coli Strain of the Gastroenteritis Microbiome
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Seung-Hak Cho, Soon Young Han, Yeon-Ho Kang
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Osong Public Health Res Perspect. 2014;5(3):156-160. Published online June 30, 2014
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DOI: https://doi.org/10.1016/j.phrp.2014.04.007
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- Objectives
To investigated whether the CTX-M-14 gene could be transferred from a clinical Shigella sonnei strain to commensal Escherichia coli strain in the gastroenteritis microbiome.
Methods
E. coli strains were isolated from 30 stool samples of S. sonnei infected students in a gastroenteritis outbreak in 2004 and were characterized by antibiotic resistance analysis, in vitro conjugation and in vivo transfer of CTX-M-14 gene and molecular assays.
Results
One strain of Escherichia coli that had high levels of resistance to cefotaxime was isolated from a patient infected with S. sonnei. Isoelectric focusing showed that the E. coli and S. sonnei strains produced a β-lactamase with an isoelectric point of 8.1. Moreover, polymerase chain reaction analysis indicated that both strains possessed the same DNA sequences for CTX-M-14. The results of in vitro and in vivo conjugation showed that the efficiency of CTX-M-14 transfer from S. sonnei to E. coli was similar to CTX-M-14 transfer between E. coli strains.
Conclusion
The data suggest that the acquisition of the extended-spectrum β-lactamases gene by pathogenic bacteria in the human intestinal tract to commensal microbiome bacteria can cause serious infectious diseases.
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Citations
Citations to this article as recorded by  - Healthy broilers disseminate antibiotic resistance in response to tetracycline input in feed concentrates
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Journal of Pure and Applied Microbiology.2017; 11(2): 779. CrossRef
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