- Possibility of CTX-M-14 Gene Transfer from Shigella sonnei to a Commensal Escherichia coli Strain of the Gastroenteritis Microbiome
-
Seung-Hak Cho, Soon Young Han, Yeon-Ho Kang
-
Osong Public Health Res Perspect. 2014;5(3):156-160. Published online June 30, 2014
-
DOI: https://doi.org/10.1016/j.phrp.2014.04.007
-
-
2,159
View
-
13
Download
-
3
Citations
-
 Abstract
 PDF
- Objectives
To investigated whether the CTX-M-14 gene could be transferred from a clinical Shigella sonnei strain to commensal Escherichia coli strain in the gastroenteritis microbiome.
Methods
E. coli strains were isolated from 30 stool samples of S. sonnei infected students in a gastroenteritis outbreak in 2004 and were characterized by antibiotic resistance analysis, in vitro conjugation and in vivo transfer of CTX-M-14 gene and molecular assays.
Results
One strain of Escherichia coli that had high levels of resistance to cefotaxime was isolated from a patient infected with S. sonnei. Isoelectric focusing showed that the E. coli and S. sonnei strains produced a β-lactamase with an isoelectric point of 8.1. Moreover, polymerase chain reaction analysis indicated that both strains possessed the same DNA sequences for CTX-M-14. The results of in vitro and in vivo conjugation showed that the efficiency of CTX-M-14 transfer from S. sonnei to E. coli was similar to CTX-M-14 transfer between E. coli strains.
Conclusion
The data suggest that the acquisition of the extended-spectrum β-lactamases gene by pathogenic bacteria in the human intestinal tract to commensal microbiome bacteria can cause serious infectious diseases.
-
Citations
Citations to this article as recorded by  - Antibiotics in avian care and husbandry-status and alternative antimicrobials
Adam Lepczyński, Agnieszka Herosimczyk, Mateusz Bucław, Michalina Adaszyńska-Skwirzyńska
Physical Sciences Reviews.2023;[Epub] CrossRef - Healthy broilers disseminate antibiotic resistance in response to tetracycline input in feed concentrates
S. Sreejith, Shamna Shajahan, P.R. Prathiush, V.M. Anjana, Arathy Viswanathan, Vishnu Chandran, G.S. Ajith Kumar, R. Jayachandran, Jyothis Mathew, E.K. Radhakrishnan
Microbial Pathogenesis.2020; 149: 104562. CrossRef - Molecular Characterization of Resistance Genes in MDR-ESKAPE Pathogens
Masoumeh Navidinia, Mehdi Goudarzi, Samira Molaei Rameshe, Zahra Farajollahi, Pedram Ebadi Asl, Saeed Zaka khosravi, Mohammad Reza Mounesi
Journal of Pure and Applied Microbiology.2017; 11(2): 779. CrossRef
|
