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Sobhan Ghafourian 5 Articles
Corrigendum
Corrigendum to “Traditional and Modern Cell Culture in Virus Diagnosis”[Osong Public Health Res Perspect 2016;7(2):77–82]
Ali Hematian, Nourkhoda Sadeghifard, Reza Mohebi, Morovat Taherikalani, Abbas Nasrolahi, Mansour Amraei, Sobhan Ghafourian
Osong Public Health Res Perspect. 2020;11(4):266-266.   Published online August 31, 2020
577
Review Article
Traditional and Modern Cell Culture in Virus Diagnosis
Ali Hematian, Nourkhoda Sadeghifard, Reza Mohebi, Morovat Taherikalani, Abbas Nasrolahi, Mansour Amraei, Sobhan Ghafourian
Osong Public Health Res Perspect. 2016;7(2):77-82.   Published online April 30, 2016
Cell cultures are developed from tissue samples and then disaggregated by mechanical, chemical, and enzymatic methods to extract cells suitable for isolation of viruses. With the recent advances in technology, cell culture is considered a gold standard for virus isolation. This paper reviews the evolution of cell culture methods and demonstrates why cell culture is a preferred method for identification...
Original Article
Anti-biofilm Properties of Peganum harmala against Candida albicans
Elham Aboualigalehdari, Nourkhoda Sadeghifard, Morovat Taherikalani, Zaynab Zargoush, Zahra Tahmasebi, Behzad Badakhsh, Arman Rostamzad, Sobhan Ghafourian, Iraj Pakzad
Osong Public Health Res Perspect. 2016;7(2):116-118.   Published online April 30, 2016
Objectives Vaginitis still remains as a health issue in women. It is notable that Candida albicans producing biofilm is considered a microorganism responsible for vaginitis with hard to treat. Also, Peganum harmala was applied as an anti fungal in treatment for many infections in Iran. Therefore, this study goal to investigate the role of P. harmala in inhibition of biofilm formation...
Cloning, Expression, and Purification of Hyperthermophile α-Amylase from Pyrococcus woesei
Amir Ghasemi, Sobhan Ghafourian, Sedighe Vafaei, Reza Mohebi, Maryam Farzi, Morovat Taherikalani, Nourkhoda Sadeghifard
Osong Public Health Res Perspect. 2015;6(6):336-340.   Published online December 31, 2015
Objectives In an attempt α-amylase gene from Pyrococcus woesei was amplified and cloned into a pTYB2 vector to generate the recombinant plasmid pTY- α-amylase. Methods Escherichia coli BL21 used as a host and protein expression was applied using IPTG. SDS-PAGE assay demonstrated the 100 kDa protein. Amylolytic activity of proteins produced by transformed E. coli cells was detected by zymography, and the rate of...
High Prevalence of AmpC β-Lactamases in Clinical Isolates of Escherichia coli in Ilam, Iran
Abbas Maleki, Afra Khosravi, Sobhan Ghafourian, Iraj Pakzad, Shiva Hosseini, Rashid Ramazanzadeh, Nourkhoda Sadeghifard
Osong Public Health Res Perspect. 2015;6(3):201-204.   Published online June 30, 2015
Objectives Widespread use of β-lactam antibiotics could cause resistance to this group of antibiotics in pathogenic bacteria through the production of the enzyme β-lactamases. The aim of this study is to determine the molecular detection of AmpC β-lactamases among clinical Escherichia coli isolated from Ilam hospitals in Ilam, Iran. Methods One hundred and twelve clinical isolates of E. coli were collected from hospitalized...

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